Amph_00130 Amph_00130   Amph_00150 Amph_00150

Amph_00140 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction65,775 / 64,324 / - [in whole cluster]
65,775 / 64,324 / - [in contig]
Locationcomplement(64324..65775) [in whole cluster]
complement(64324..65775) [in contig]
TypeCDS
Length1,452 bp (483 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductGDP-mycosamine glycosyltransferase
Product (GenBank)AmphDI
Gene
Gene (GenBank)amphDI
EC number
Keyword
  • mycosamine
Note
Note (GenBank)
  • probably glycosylates amphotericin aglycone with mycosamine
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
[18798210] The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI. (Chembiochem. , 2008)
[20189107] Redesign of polyene macrolide glycosylation: engineered biosynthesis of 19-(O)-perosaminyl-amphoteronolide B. (Chem Biol. , 2010)
[23324745] In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin. (Mol Biosyst. , 2013)
comment
[PMID: 11451671](2001)
Amphotericin生合成gene clusterの報告。
amphDI: Glycosyl transferase

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[PMID: 18798210](2008)
polyene glycosyltransferases AmphDI and NysDIの過剰発現、精製、in vitro characterization.

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[PMID: 20189107](2010)
S. aminophilusはpolyeneの糖としては珍しいperosamineを含むperimycinを産生する。
S. aminophilus perDII perosamine synthaseをS. nodosus ΔamphDII mutantに導入すると19-O-perosaminyl-amphoteronolide Bが得られたが、その収量はとても少なかった。

これはAmphDI の糖基質特異性に関連すると考えられたことから、AmphDI のputative 8-deoxyamphoteronolide-binding N-terminal domainが、PerDI のputative GDP-perosamine-binding C-terminal domainと融合されたhybrid GTsが構築された。hybrid GTsがperDII と共にΔamphDII mutantで発現されると、19-O-perosaminyl-amphoteronolide Bが効率的に産生された。

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[PMID: 23324745](2013)
abstract:
putative glycosyltransferase(GT)をコードするfscMI のmutant株で、amphDI, nysDI, pimKなどの相同なGT genesは、candicidin aglycone → candicidin/FR-008-III への変換を触媒できる。

また、PKSのDH11 domainの不活化でpolyketide骨格が変化してもmycosamineを持つ新規polyene派生体が導かれ、これらpolyene GTsの基質特異性がゆるいことが確認された。

close this sectionSequence

selected fasta
>GDP-mycosamine glycosyltransferase [AmphDI]
MGGREACGPARSATFRRVLSMGAHRRPILFVSYAESGLLNPLLVLAEELSRRGVEDLWFA
TDEKARDQIESASADSELQFASLGDTVSQMSAVTWDDETYAEVTQRSRFKAHRAVIRHSF
APETRVEKYRALEKAVEEIQPALMVIESMCQFGYELAITKGIPFVLGVPFLPSNVLTSHV
PFAKSYTPSGFPVPHSGLPGKMSLAQRVENELFRVRTLGMFMTKEIREIVEEDNRVRGEL
GISPEARQMMARIDHAEQVLCYSVAELDYPFPMHEKVRLVGTLVPPLPQAPDDEGLSDWL
TEQKSVVFMGFGTITRLTREQVASLVEVARRLEGEGHQVLWKLPSEQQHLLPPAEELPAN
LRIESWVPSQLDVLAHPNVKVFFTHAGGNGYHEGLYFGKPLVVRPLWVDCDDQAVRGQDF
GVSLTVDRPETVDTDDVLDKITRVLNESSFTERAEYYAGLLKAAGGRTAAADLLLGLPVL
AND
selected fasta
>GDP-mycosamine glycosyltransferase [AmphDI]
GTGGGTGGACGCGAGGCGTGCGGGCCTGCGCGGTCCGCCACTTTCCGGAGGGTGTTGTCC
ATGGGCGCGCACAGGCGGCCGATCCTGTTCGTCAGTTATGCCGAAAGCGGTCTTCTCAAT
CCGCTGCTCGTACTGGCCGAGGAATTGTCCCGGCGTGGTGTGGAGGATCTGTGGTTCGCC
ACGGACGAGAAGGCGCGGGACCAGATCGAGTCGGCGTCGGCGGACAGTGAGCTGCAGTTC
GCCTCGCTCGGTGACACCGTGTCGCAGATGTCGGCGGTCACCTGGGACGACGAGACCTAT
GCCGAGGTGACGCAGCGCTCCCGCTTCAAGGCGCACCGCGCGGTCATCCGGCACTCCTTC
GCGCCCGAGACCCGGGTGGAGAAGTACCGGGCGCTGGAGAAGGCCGTCGAGGAGATCCAG
CCCGCCCTGATGGTGATCGAGAGCATGTGCCAGTTCGGGTACGAGCTGGCGATCACCAAG
GGGATCCCGTTCGTGCTCGGGGTGCCGTTCCTGCCGAGCAATGTACTGACCTCGCATGTG
CCGTTCGCCAAGTCGTACACGCCGTCCGGGTTCCCGGTGCCGCACTCGGGCCTGCCCGGC
AAGATGTCGCTCGCCCAGCGGGTGGAGAACGAGCTGTTCCGGGTGCGCACGCTCGGGATG
TTCATGACGAAGGAGATCCGGGAGATCGTCGAGGAGGACAACCGGGTCCGCGGCGAGCTG
GGCATCTCCCCCGAGGCGCGGCAGATGATGGCGCGCATCGACCACGCCGAGCAGGTGCTG
TGCTACTCGGTGGCGGAGCTGGACTACCCGTTCCCGATGCACGAGAAGGTACGGCTGGTG
GGCACGCTGGTGCCGCCGCTGCCGCAGGCGCCGGACGACGAGGGCCTCTCGGACTGGCTG
ACGGAGCAGAAGTCCGTGGTCTTCATGGGCTTCGGCACCATCACCCGGCTCACCCGGGAG
CAGGTCGCCTCGCTGGTGGAGGTGGCCCGCCGCCTCGAGGGCGAGGGCCACCAGGTGCTG
TGGAAGCTGCCGAGCGAGCAGCAGCATCTGCTGCCCCCGGCCGAGGAACTGCCCGCGAAC
CTGCGGATCGAGAGCTGGGTGCCCTCACAGCTGGACGTGCTGGCGCACCCGAACGTCAAG
GTGTTCTTCACGCACGCCGGCGGCAACGGCTACCACGAGGGCCTGTACTTCGGAAAGCCT
CTGGTGGTACGGCCGTTGTGGGTGGACTGCGACGACCAGGCCGTGCGCGGCCAGGACTTC
GGCGTGAGCCTGACCGTCGACCGTCCCGAGACCGTGGACACGGACGACGTCCTCGACAAG
ATCACCCGCGTGCTGAACGAGTCCTCCTTCACCGAACGCGCCGAGTACTACGCCGGGTTG
CTGAAGGCCGCGGGTGGTCGTACCGCCGCCGCGGACCTGCTGCTCGGCCTCCCCGTCCTG
GCAAACGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family)
[109-453] 8.59999999999999e-29 PF00201
PF00201   UDPGT
SignalP
[1-46] 0.531 Signal
Eukaryota   
[1-33] 0.338 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Amph_00130 Amph_00130   Amph_00150 Amph_00150
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