Amph_00140 Amph_00140   Amph_00160 Amph_00160

Amph_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction66,081 / 70,319 / + [in whole cluster]
66,081 / 70,319 / + [in contig]
Location66081..70319 [in whole cluster]
66081..70319 [in contig]
TypeCDS
Length4,239 bp (1,412 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)AmphA
Gene
Gene (GenBank)amphA
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase multienzyme polypeptide housing loading module
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
comment
Amphotericin生合成gene clusterの報告。
amphA: PKS protein, loading module

LMのdomain構造はKS(S)-AT-DH-ACP.
KS domainのactive siteはcysteine→serineに置換されている。
acetyl starter unitsを生成するため、KS(S) domainがmalonyl-ACP decarboxylaseとして働くかもしれない。

close this sectionPKS/NRPS Module

0 acetyl-CoA
KS8..380
AT574..888
dh931..1096
ACP1255..1329

close this sectionSequence

selected fasta
>polyketide synthase [AmphA]
MTIGANDDPVVVVGMACRFPGGVEGPEDLWELVRDGRDATGPFPGDRGWDLAALTGDGPD
HSVTHRGGFLAAAADFDAGFFGMSPREAVSTDPQQRLVLETSWEALEHAGIDPHTLRGTR
TGVFVGTNGQDYATVTNASREDLTGHALTGLSPSIASGRLAYFLGLEGPAVTLDTASSSS
LVALHYALRSLRSGECTTALAGGVTVMSTPVGFIAYTRQGGLAADGRCKVFSDDADGTTW
AEGAGMIVLERLSTARAAGHRVLAVLRGSAVNQDGASDGLTAPSGPAQERLVREALADAG
LGPADIDLVEAHGTGTRLGDPIEARALLATYGQDRDGGQPLRLGSLKSNIGHAQAAAGIG
GLIKAVQALRHGLMPETLNLSTPTRHVDWSAGAVELLTEALPWPGTGRPRRAAVSSFGIS
GTNAHVIVEEAPTTDPAAAVPAGPAHRDVASAADSAARPAALAGEPADTSAPAAVDAGPA
DRPVTPAARLAALVPAADAVAWPVSGASPEALDAQVERLTSFVRDHPGADPLDIGHSLAT
GRAALRHRAVLVPSGDGVVEIARGEAAPRTTAVLFSGQGSQRLGMGRELAARFPVFAKAL
DTVLAALDPQLERPVRSVMWGEDPAELDRTGWTQPALFAFEVALYRLAESFGLRPDAVGG
HSVGEIAAAHIAGVLSLEDAARLVAARATLMQALPEGGAMSAVEASEDEVLPLLDGDVSL
AAVNGPTAVVVSGAEDAVERVSAHFAAQGRRTSRLAVSHAFHSPLMEPMLDAFRDVVAGL
TFHEPTLPVMSNLTGELAGAEIATPEYWVRHVRGTVRFADGVTALREHGTDLLVELGPGS
VLTALARTVLGPDTPGAPVDVVPTLRKDQPEERALTAALGRLHVLGATVDWSALYTGTGA
RRTDLPTYAFQHARYWPAPGRPGTGTAGGGHPLLGPAVELADGGTVSGATLSVATHPWLA
DHVVAGRVLLPAAVLVELAVRAGDDTGCDVLHELALVEAPVLEAGDTLDLQVRVGSADEA
GRRTLTVHSRPGNSPAEPWTQRAGGLLGTAPRTAAAPDTSFAVAWPPPGAEPLDLGDHYE
RLVDDGFDLGPAFRGLRTAWRHDGAFLAEVELPAGTTDDPGAYGVHPALLDAARHAALTT
TGTLPVAWHGVRLHAVGATALRVRIHSADDGALTLTAADVTGAPVFTAEAVVVRQLTEQE
RTAPRPLTRAWHQDTATPRRTRPVAAAPGAAAEPSASSAPDSFAAEAAALAPAERERRLI
GLVRTQAAAVLGHQGPDAVGPRAVFKELGFDSLAGVELSDRLTALTGLRLPATLVFNFPT
PELAARRIGELLVVSGSSPQGSCDDELTRFEAVVQTLSADDPGRQAVADRLDALVASLRR
NSAPQENFSDEDIESVSVDRLLDIIDEEFEIS
selected fasta
>polyketide synthase [AmphA]
ATGACGATCGGAGCCAACGACGATCCGGTAGTGGTCGTCGGAATGGCCTGCCGCTTCCCG
GGAGGCGTCGAGGGGCCCGAGGACCTGTGGGAGCTGGTCCGCGACGGCCGCGACGCCACC
GGGCCGTTCCCCGGCGACCGCGGCTGGGACCTGGCCGCCCTGACCGGCGACGGGCCGGAC
CACAGCGTGACCCACCGAGGCGGATTCCTCGCCGCGGCCGCCGACTTCGACGCCGGCTTC
TTCGGGATGTCGCCCCGCGAGGCCGTCTCCACCGACCCGCAGCAGCGGCTCGTCCTGGAG
ACCTCCTGGGAGGCCCTGGAACACGCCGGCATCGACCCGCACACCCTGCGGGGCACCCGC
ACCGGCGTCTTCGTCGGCACCAACGGCCAGGACTACGCGACCGTCACCAACGCCTCCCGC
GAGGACCTCACCGGGCACGCCCTCACCGGTCTGTCGCCGAGCATCGCCTCCGGGAGGCTC
GCCTACTTCCTCGGCCTCGAAGGGCCCGCCGTCACCCTCGACACGGCGTCCTCCTCGTCC
CTGGTCGCCCTCCACTACGCGCTGCGCTCGCTCAGGTCGGGGGAGTGCACCACCGCGCTG
GCCGGCGGCGTCACCGTGATGTCCACACCGGTCGGGTTCATCGCCTACACCCGGCAGGGC
GGACTCGCCGCCGACGGCCGCTGCAAGGTCTTCTCCGACGACGCCGACGGCACCACCTGG
GCGGAGGGCGCCGGCATGATCGTGCTGGAGCGCCTGTCCACCGCCCGCGCCGCCGGGCAC
CGGGTGCTCGCCGTGCTGCGCGGCTCCGCCGTCAACCAGGACGGCGCCTCCGACGGTCTC
ACCGCCCCCAGCGGACCGGCCCAGGAACGACTCGTCCGCGAGGCCCTCGCCGACGCCGGA
CTCGGACCCGCCGACATCGACCTCGTCGAGGCCCACGGCACCGGCACCCGGCTCGGCGAC
CCCATCGAGGCCCGGGCCCTGCTCGCCACCTACGGCCAGGACCGCGACGGCGGACAGCCG
CTGCGCCTCGGCTCCCTGAAGTCCAACATCGGGCACGCCCAGGCAGCCGCCGGCATCGGC
GGACTCATCAAGGCCGTCCAGGCGCTGCGCCACGGCCTGATGCCGGAGACCCTGAACCTC
TCCACGCCCACCCGGCACGTCGACTGGTCGGCCGGCGCCGTCGAACTCCTCACCGAGGCC
CTGCCCTGGCCCGGCACCGGCCGCCCGCGCCGGGCCGCCGTCTCCTCCTTCGGCATCAGT
GGCACCAACGCGCATGTCATCGTGGAGGAAGCCCCGACGACCGACCCCGCCGCCGCGGTC
CCCGCCGGGCCCGCCCACCGGGACGTGGCCTCGGCCGCCGACTCCGCCGCGCGGCCCGCT
GCCCTCGCCGGGGAGCCCGCCGACACCTCTGCTCCCGCCGCTGTCGACGCCGGCCCGGCC
GACCGCCCGGTCACCCCCGCCGCTCGGCTCGCCGCCCTCGTGCCCGCGGCCGACGCCGTC
GCGTGGCCGGTGTCCGGGGCCTCCCCGGAGGCTCTCGACGCGCAGGTCGAGCGGCTCACC
TCCTTCGTCCGGGACCACCCCGGCGCCGATCCGCTGGACATCGGTCACTCGCTGGCCACC
GGGCGGGCGGCGTTGCGGCACCGTGCGGTGCTGGTGCCGTCCGGTGACGGTGTCGTGGAG
ATCGCCCGCGGTGAGGCCGCCCCCCGCACCACCGCCGTCCTCTTCTCCGGACAGGGCTCC
CAGCGGCTCGGCATGGGCCGTGAACTCGCCGCCCGCTTCCCGGTCTTCGCGAAGGCCCTG
GACACCGTCCTGGCCGCCCTCGACCCCCAACTCGAGCGTCCGGTGCGGTCCGTGATGTGG
GGCGAGGACCCCGCCGAACTCGACCGCACCGGGTGGACCCAGCCCGCGCTGTTCGCCTTC
GAGGTCGCCCTGTACCGGCTCGCCGAGTCCTTCGGGCTGCGCCCCGACGCCGTCGGCGGC
CACTCCGTCGGCGAGATCGCCGCCGCGCACATCGCCGGAGTGCTCTCGCTGGAGGACGCC
GCGCGTCTGGTCGCCGCCCGCGCCACCCTGATGCAGGCCCTGCCCGAGGGCGGCGCCATG
TCCGCCGTCGAGGCCTCCGAGGACGAGGTGCTCCCGCTGCTCGACGGCGATGTCTCGCTC
GCCGCCGTCAACGGCCCCACCGCGGTCGTCGTCTCCGGCGCCGAGGACGCCGTGGAGCGC
GTCTCCGCCCACTTCGCTGCCCAAGGCCGCCGCACCAGCCGCCTCGCGGTCTCGCACGCC
TTCCACTCGCCGCTGATGGAGCCGATGCTCGACGCCTTCCGGGACGTCGTCGCCGGACTC
ACCTTCCATGAGCCGACGCTGCCGGTGATGTCCAACCTCACCGGTGAACTTGCCGGTGCC
GAGATCGCCACCCCCGAGTACTGGGTGCGGCATGTGCGCGGTACCGTCCGCTTCGCCGAC
GGCGTGACGGCCCTGCGGGAACACGGCACCGACCTGCTGGTCGAACTGGGCCCCGGCAGC
GTCCTGACCGCCCTCGCCCGCACCGTCCTCGGCCCGGACACCCCGGGCGCCCCTGTCGAC
GTGGTGCCCACCCTCCGCAAGGACCAGCCCGAGGAGAGGGCTCTCACCGCCGCGCTCGGC
CGGCTCCATGTCCTCGGCGCGACCGTCGACTGGTCCGCCCTCTACACCGGCACCGGAGCC
CGCCGCACCGACCTGCCGACGTACGCCTTCCAGCACGCGCGGTACTGGCCCGCCCCGGGC
CGGCCCGGCACCGGTACCGCGGGCGGCGGGCATCCGCTGCTCGGCCCGGCCGTGGAACTC
GCCGACGGCGGCACGGTGTCGGGCGCCACACTGTCCGTCGCCACCCACCCCTGGCTCGCC
GACCATGTCGTCGCCGGGCGCGTCCTGCTGCCCGCCGCCGTGCTCGTGGAACTCGCCGTA
CGCGCGGGTGACGACACCGGATGCGACGTCCTGCACGAACTCGCCCTCGTCGAGGCGCCG
GTCCTGGAGGCCGGCGACACCCTGGACCTCCAGGTCCGGGTCGGCTCCGCCGACGAGGCC
GGCCGGCGCACCCTCACCGTCCACTCCCGTCCCGGCAACTCCCCCGCCGAGCCCTGGACC
CAGCGGGCCGGCGGCCTGCTCGGCACCGCGCCCCGCACCGCGGCGGCCCCCGACACCTCC
TTCGCCGTCGCCTGGCCCCCGCCGGGCGCCGAACCCCTCGACCTCGGGGACCACTACGAG
CGGCTCGTCGACGACGGCTTCGACCTCGGCCCCGCCTTCCGCGGTCTGCGCACCGCCTGG
CGCCACGACGGCGCGTTCCTCGCCGAGGTCGAACTCCCGGCCGGCACCACCGACGACCCC
GGCGCCTACGGAGTGCACCCCGCGCTCCTCGACGCCGCCCGGCACGCCGCCCTCACCACC
ACCGGCACACTCCCGGTCGCCTGGCACGGGGTGCGGCTGCACGCCGTCGGCGCCACCGCC
CTGCGGGTGCGGATCCACTCCGCCGACGACGGTGCCCTGACCCTGACCGCCGCCGATGTC
ACCGGCGCCCCGGTGTTCACCGCCGAGGCCGTCGTCGTACGGCAGCTCACCGAGCAGGAG
CGCACCGCCCCCCGGCCGCTCACACGCGCCTGGCACCAGGACACCGCGACCCCGCGCCGC
ACCCGGCCCGTCGCCGCGGCCCCCGGCGCCGCCGCCGAGCCGTCCGCCTCCTCGGCGCCG
GACAGCTTCGCCGCCGAGGCCGCGGCCCTGGCCCCCGCCGAGCGTGAACGCCGGCTCATC
GGCCTGGTACGGACCCAGGCGGCGGCGGTCCTCGGCCATCAGGGCCCGGACGCGGTCGGA
CCCCGCGCGGTCTTCAAGGAGCTGGGCTTCGACTCGCTGGCCGGCGTGGAACTCAGTGAC
CGCCTCACCGCGCTCACCGGACTGCGGCTGCCGGCCACCCTCGTCTTCAACTTCCCCACC
CCCGAGCTCGCCGCCCGGCGTATCGGGGAACTCCTCGTCGTATCCGGCTCCTCACCGCAG
GGATCGTGCGACGACGAACTCACCAGGTTCGAGGCCGTCGTGCAGACCCTGTCGGCCGAC
GACCCCGGACGCCAGGCCGTCGCCGACCGCCTGGACGCACTCGTCGCCTCGCTCCGGCGG
AATTCCGCCCCGCAGGAGAACTTCTCCGACGAGGACATCGAATCGGTGTCGGTCGACAGA
CTGCTCGACATCATCGATGAAGAGTTCGAAATCTCCTAG
[0] KS8..380
[0] AT574..888
[0] acetyl-CoA759..763
[0] dh931..1096
[0] ACP1255..1329
[0] KS22..1140
[0] AT1720..2664
[0] acetyl-CoA2275..2289
[0] dh2791..3288
[0] ACP3763..3987

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[561-694] 8.10000000000001e-76 G3DSA:3.40.366.10 [759-877] 8.10000000000001e-76 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1287-1302] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1263-1326] 5.10000000000001e-08 PF00550
PF00550   PP-binding
[1255-1329] PS50075
PS50075   ACP_DOMAIN
[1251-1332] 9.59999914716171e-18 SSF47336
SSF47336   ACP_like
[1260-1330] 2.80000000000001e-16 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[8-255] 1.5e-88 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[263-380] 6.6999999999999e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[574-888] 1.10000000000001e-67 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[570-869] 1.90000694315261e-72 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[696-758] 1.20000117458134e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[8-265] 3.79999999999991e-87 G3DSA:3.40.47.10 [267-433] 1.9e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[7-377] 6.69996745106047e-94 SSF53901
SSF53901   Thiolase-like
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[574-869] 8.19994104549873e-124 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1260-1332] 2.39999798157265e-27 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[931-1096] 1.79999754022375e-64 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[10-433] SM00825
SM00825   PKS_KS
SignalP
[1-25] 0.105 Signal
Eukaryota   
TMHMM No significant hit
Amph_00140 Amph_00140   Amph_00160 Amph_00160
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