Borre_00160 : CDS information

Location

Organism	Streptomyces parvulus
Strain	Tü4055
Entry name	Borrelidin
Contig	AJ580915
Start / Stop / Direction	18,875 / 23,590 / + [in whole cluster] 18,875 / 23,590 / + [in contig]
Location	18875..23590 [in whole cluster] 18875..23590 [in contig]
Type	CDS
Length	4,716 bp (1,571 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	borrelidin polyketide synthase, type I
Gene
Gene (GenBank)	borA2
EC number
Keyword
Note
Note (GenBank)	Module 1 (b-ketoacyl-ACP-synthase, acyltransferase, ketoreductase, acylcarrier protein)
Reference	ACC Q70I01 PmId [15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004) [14651102] Evidence from engineered gene fusions for the repeated use of a module in a modular polyketide synthase. (Chem Commun (Camb). , 2003) [25188840] In vitro analysis of carboxyacyl substrate tolerance in the loading and first extension modules of borrelidin polyketide synthase. (Biochemistry. , 2014) [26501439] Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid. (ACS Synth Biol. , 2016) [26976746] Alteration of Polyketide Stereochemistry from anti to syn by a Ketoreductase Domain Exchange in a Type I Modular Polyketide Synthase Subunit. (Biochemistry. , 2016) comment [PMID: 15112998](2004) Borrelidin生合成gene clusterのクローニング。 borA2: PKS module 1(KS-ATa-KR-ACP) --- [PMID: 14651102](2003) 同じ人たちの先行する論文。 PKSの反復利用について報告している。 --- [PMID: 25188840](2014) 異種性発現、精製して得たBorA1(loading module)とBorA2(最初の伸長module)を用いて、それぞれの基質特異性を確認している。 BorA1はカルボキシ部分を含む基質を要求するが、BorA2は広い基質寛容を示した。 --- [PMID: 26501439](2016) borrelidin PKSのmodule 1を土台として、アジピン酸を産生できるキメラPKSを作製している。 --- [PMID: 26976746](2016) ドナーKR domainとしてBor KR1を使用。

PKS/NRPS Module

malonyl-CoA

KS	34..410

AT	558..864

KR	1138..1314

ACP	1419..1489

Sequence

>polyketide synthase [borrelidin polyketide synthase, type I]
MAHEDKLRHLLKRVSAELDDTQRRVREMEESEREPIAIVGMSCRLPGGVNSPGEFWSLLE
AGTDAVSEFPRDRGWDVENLYDPDPDAPGRSYVREGGFLDGAGQFDAAFFGISPREALAM
DPQQRLLLECSWEAIERSRIDPKTLHGSRTGVFAGSNWQDYNTLLLNAEERSQSYLATGA
SGSVLSGRVSYTLGMEGPAITVNTACSSSLVAVHLAARSLRAGECDLALAGAVTVMSTPQ
LPVAFSRQRGLAPDGRSKAFAVSADGMGFGEGVGVLVLERLSVARRNGHRVLAVVRGSAV
NQDGASNGLTAPNGPSQQRVIRAALASAGLGPADVDVVEAHGTGTRLGDPIEAQALLATY
GRGRDAERPLWLGSVKSNIGHAQAAAGVAGVIKMVLAMEKGRLPRTLHVDEPSGEVDWDS
GAVRLLTEARDWPSEEGRLRRAGVSSFGISGTNAHVIIEEAPEEGEEPESDAGGVVPWVL
SARTEGALQAQAVQLSEFVGESSPVDVGWSLVSTRAAFEHRAVVVGRGRDELVRGLSEVA
QGRGVRGVASSASGGLAFVFAGQGSQRLGMGRGLYERFPVFAEAFDEVCGRVGPGVREVV
FGSDAGELDRTVWAQAGLFALEVALFRLLESWGVRPGCLIGHSVGELSAACVAGLWSLED
ACRVVAARARLMQALPAGGVMVAVRAEAGELAGFLGEDVVIASVNAPGQVVIAGPEGGVE
RVVAACGARSRRLAVSHAFHSPLVEPMLGEFRRVVESVAFGVPSLRVVSNVTGAWVDPEE
WGTPEYWVRQVREPVRFADGVATLLDAGVRTFVELGPAGALTSMVSHCADATATSVTAVP
TLRPDHDESRTVLSAAASLYVQGHPVDWAPLFPRARTVDLPTYPFQHQHYWLDVPPLFTA
SSAAQDGGWRYRIHWRRLGTRDSGDRLSGRWLLLVPESDGTEPWVEGAEKMLAERGCEVV
HVPIAATADRDAMVGAVRESVEDGRVDGVLSLLALDGRPHPDAAAVPTGLVATAQVVQVS
DELGIGPLWVATRQAVSVDGADEADGAGRTRKADDPADVAQAAVWGLGRVAALEKPRLWG
GLVDLPARADERMRDLVAQALTAPDAEDQLAVRADGIAVRRLVRSAASAPADDWQPSGTV
LVTGGTGGVGANVARWLVTQDIQHLLLVSRRGPDAPGAAELLAELSASGTSVTIEPCDVT
DADAVRRLIGAVPAERPLSTVVHAAGVLDDCLIDALTPQRLAAALEVKAKGALNLHEAAG
EAHLVLFSSLAGTTGTKGQGNYAAANAYLDALAERRRADGLPATSVAWGAWQGAGMVADA
AVAHRTRRYGLPLMSPDRAVATLRQVMAEPVATQVVADVDWQRFVADFTAVRPSRLLADL
PEVRSLGEQRKDGPGGQGEEDGLASKLAALPEADRRRAVLDLVEELVLGVLGHETRAAIG
PDSSFHAIGFDSLTAVELRNLLTVRLGMKLPATLVYDHPTLSSLADHLHEQLVIDGTPMT
DTAADLLAELDALAARLAAVGLEPEARARIGRRLKDMQTACEPRSESSRDLKSASRTEVL
DFLTNELGISR

>polyketide synthase [borrelidin polyketide synthase, type I]
ATGGCACATGAAGACAAACTGCGCCACCTCCTCAAGCGTGTCAGTGCTGAACTCGACGAC
ACCCAGCGCCGGGTGCGTGAGATGGAGGAGAGCGAGCGCGAGCCGATCGCGATCGTGGGG
ATGAGCTGCCGTCTGCCCGGCGGGGTGAACAGCCCGGGGGAGTTCTGGTCGCTGCTGGAG
GCCGGGACGGACGCCGTCTCGGAGTTCCCGCGGGACCGTGGCTGGGATGTGGAGAACCTC
TACGACCCGGACCCGGACGCCCCCGGGCGGTCGTACGTCCGCGAGGGCGGATTCCTGGAC
GGGGCCGGACAGTTCGACGCCGCCTTCTTCGGAATCTCGCCCCGTGAGGCGCTGGCGATG
GATCCGCAGCAGCGGCTGCTGCTGGAGTGCTCGTGGGAGGCGATCGAGCGGTCGCGGATC
GACCCGAAGACCCTGCACGGCAGCCGGACCGGCGTCTTCGCGGGCTCCAACTGGCAGGAC
TACAACACCCTGTTGCTGAACGCCGAGGAGCGCTCCCAGAGCTACCTGGCCACCGGCGCC
TCCGGAAGCGTGCTGTCCGGGCGCGTCTCGTACACGCTGGGCATGGAAGGGCCCGCGATC
ACCGTGAACACGGCGTGCTCGTCCTCTCTGGTCGCCGTCCACCTGGCGGCCCGTTCCCTG
CGGGCGGGGGAGTGCGACCTCGCCCTGGCCGGCGCCGTCACGGTCATGTCCACACCGCAG
CTTCCGGTCGCCTTCTCCCGGCAGCGCGGACTCGCCCCTGACGGTCGCTCGAAAGCCTTC
GCGGTTTCGGCCGACGGCATGGGCTTCGGCGAGGGGGTGGGCGTGCTTGTGCTGGAGCGG
TTGTCGGTGGCGCGGCGGAACGGTCATCGGGTGTTGGCGGTGGTGCGGGGTTCGGCGGTG
AACCAGGACGGTGCGTCGAACGGTCTGACGGCGCCGAACGGTCCGTCGCAGCAGCGGGTG
ATACGTGCGGCGTTGGCGAGTGCCGGGCTGGGTCCGGCCGATGTGGATGTGGTGGAGGCG
CACGGTACGGGGACGCGGTTGGGTGATCCGATCGAGGCGCAGGCGTTGCTGGCGACGTAC
GGGCGGGGCCGGGACGCGGAGCGTCCGTTGTGGCTGGGGTCGGTGAAGTCGAACATCGGT
CATGCGCAGGCTGCTGCCGGTGTCGCCGGTGTCATCAAGATGGTGCTGGCCATGGAGAAG
GGCCGTCTCCCTCGGACGCTGCATGTGGATGAGCCGTCGGGTGAGGTGGACTGGGACTCG
GGTGCGGTGCGGCTGCTGACCGAGGCGCGGGACTGGCCGTCGGAGGAAGGTCGTCTGCGG
CGGGCCGGTGTGTCGTCGTTCGGGATCTCAGGCACCAACGCGCACGTGATCATCGAGGAA
GCACCGGAAGAGGGGGAGGAACCGGAGTCCGACGCGGGTGGTGTGGTGCCGTGGGTGCTC
TCCGCGCGGACGGAAGGGGCACTGCAAGCACAGGCGGTGCAACTGAGCGAGTTCGTCGGC
GAGTCGAGTCCGGTGGATGTGGGTTGGTCGTTGGTTTCGACGCGTGCGGCGTTCGAGCAT
CGGGCCGTGGTGGTGGGGCGCGGGCGGGACGAGTTGGTGCGGGGCTTGTCCGAGGTCGCG
CAGGGTCGGGGCGTGAGGGGTGTCGCGTCTTCGGCGTCGGGTGGTCTCGCGTTTGTTTTT
GCTGGTCAGGGCAGTCAGCGGTTGGGGATGGGGCGGGGGTTGTATGAGCGGTTCCCGGTG
TTTGCCGAGGCGTTCGACGAGGTGTGTGGGCGGGTCGGTCCGGGGGTGCGGGAGGTTGTT
TTCGGTTCGGATGCGGGTGAGTTGGACCGGACGGTGTGGGCGCAGGCGGGGTTGTTCGCG
TTGGAGGTGGCGCTGTTTCGGTTGTTGGAGTCCTGGGGTGTGCGGCCGGGTTGTCTGATC
GGGCATTCGGTCGGTGAGTTGTCGGCGGCGTGTGTGGCGGGGTTGTGGTCGTTGGAGGAT
GCGTGTCGGGTCGTGGCTGCCCGGGCGCGGTTGATGCAGGCGTTGCCGGCGGGTGGGGTG
ATGGTCGCGGTTCGGGCCGAGGCGGGGGAGCTGGCCGGTTTCCTCGGTGAGGACGTGGTG
ATCGCGTCGGTGAACGCGCCGGGGCAGGTGGTGATCGCTGGTCCTGAGGGGGGTGTGGAG
CGTGTGGTGGCTGCTTGTGGGGCGCGGTCGCGTCGTCTGGCGGTCTCGCATGCTTTTCAT
TCGCCTTTGGTGGAGCCGATGCTTGGGGAGTTCCGTCGGGTTGTGGAGTCGGTGGCGTTC
GGTGTGCCGTCGTTGCGGGTGGTTTCCAATGTCACGGGTGCGTGGGTGGATCCGGAGGAG
TGGGGGACGCCGGAGTACTGGGTGCGTCAGGTCCGTGAGCCGGTGCGTTTCGCCGACGGG
GTCGCCACGTTGCTCGACGCGGGTGTGAGGACGTTCGTCGAGCTGGGTCCCGCCGGGGCG
CTCACTTCGATGGTCAGCCACTGCGCGGACGCCACCGCCACTTCGGTGACGGCTGTACCT
ACCTTGCGCCCCGATCACGATGAGTCGCGGACCGTGTTGAGTGCCGCAGCGTCCTTGTAC
GTCCAGGGTCACCCGGTCGACTGGGCCCCGCTGTTCCCGCGGGCCCGCACGGTGGACCTG
CCCACCTACCCCTTCCAGCACCAGCACTACTGGCTCGACGTACCTCCTCTGTTCACCGCC
TCCTCGGCGGCCCAGGACGGTGGCTGGCGATACCGCATCCACTGGCGGCGGCTCGGCACG
AGGGACTCCGGGGACCGGCTCTCCGGCCGCTGGTTGCTGCTGGTGCCCGAGTCGGACGGG
ACGGAGCCCTGGGTGGAGGGGGCCGAGAAGATGCTGGCCGAGCGCGGGTGCGAAGTCGTC
CACGTGCCGATCGCGGCGACGGCCGACCGGGACGCGATGGTCGGAGCCGTGCGTGAGAGC
GTCGAGGACGGTCGGGTCGACGGTGTGCTCAGCCTGCTGGCGCTCGACGGCCGCCCGCAC
CCCGATGCGGCTGCGGTGCCGACAGGGTTGGTCGCCACGGCGCAGGTTGTGCAGGTCAGT
GACGAGCTGGGCATCGGCCCGCTGTGGGTCGCCACCCGACAGGCGGTCTCCGTCGACGGG
GCCGATGAGGCTGACGGGGCCGGTAGGACCAGGAAGGCCGACGACCCCGCCGATGTCGCG
CAGGCCGCTGTGTGGGGGCTCGGCCGGGTCGCCGCGCTGGAGAAGCCTCGGTTGTGGGGC
GGCCTCGTCGACCTGCCCGCACGTGCCGACGAACGGATGCGGGACCTGGTGGCTCAGGCC
CTCACCGCTCCCGACGCCGAGGACCAACTTGCCGTGCGGGCCGACGGCATCGCCGTTCGC
CGACTGGTACGCTCCGCCGCGTCGGCCCCGGCCGACGACTGGCAGCCGAGCGGCACCGTG
CTGGTCACCGGCGGCACCGGAGGCGTCGGAGCCAACGTGGCGCGTTGGCTGGTCACCCAG
GACATCCAGCACCTGTTGCTGGTCAGCCGGCGCGGCCCGGACGCCCCCGGAGCCGCTGAG
CTGCTGGCCGAACTCAGCGCCTCAGGAACGTCCGTGACCATCGAGCCCTGCGACGTCACC
GACGCGGACGCGGTACGGCGCCTGATCGGCGCCGTACCGGCCGAACGGCCGCTGAGCACG
GTCGTCCACGCCGCGGGCGTACTGGACGACTGCTTGATCGACGCCCTGACCCCGCAGCGC
CTCGCCGCCGCACTGGAGGTCAAGGCCAAGGGCGCACTGAACCTCCACGAGGCGGCCGGG
GAAGCCCACTTGGTGCTCTTCTCCTCGCTGGCCGGAACAACCGGAACCAAGGGACAGGGC
AACTACGCCGCCGCAAACGCCTATCTCGACGCTCTGGCCGAACGGCGGCGTGCTGACGGC
CTGCCCGCCACTTCGGTCGCCTGGGGCGCCTGGCAGGGCGCGGGCATGGTGGCCGACGCC
GCCGTAGCCCACCGCACGCGCCGTTATGGCCTCCCGCTCATGAGCCCCGACCGCGCCGTC
GCCACCCTGCGGCAGGTCATGGCCGAGCCGGTGGCCACGCAGGTGGTGGCGGACGTCGAC
TGGCAGCGATTCGTCGCCGACTTCACCGCGGTGCGCCCCAGCCGCCTCCTCGCCGACCTG
CCGGAAGTGCGCTCCCTGGGCGAGCAGCGAAAGGACGGCCCGGGCGGTCAGGGCGAGGAG
GACGGCTTGGCCAGCAAGCTGGCAGCCCTGCCCGAAGCCGACCGCCGACGAGCCGTGCTG
GACCTCGTGGAGGAACTCGTCCTCGGGGTTCTGGGCCACGAGACGCGCGCGGCGATCGGC
CCGGACAGTTCCTTCCACGCCATCGGCTTCGACTCGCTCACCGCCGTCGAACTGCGCAAC
CTGCTGACCGTACGCCTCGGGATGAAGCTGCCCGCGACCCTCGTCTACGATCACCCGACC
CTGTCGTCGCTGGCCGACCACCTGCACGAGCAACTGGTTATCGACGGCACCCCCATGACG
GACACCGCGGCCGACCTGCTCGCCGAACTCGACGCACTCGCGGCGAGACTCGCCGCCGTC
GGGCTGGAACCGGAGGCGCGCGCCCGCATCGGACGCAGGCTCAAGGACATGCAGACCGCC
TGCGAACCCAGGTCGGAGTCCTCACGCGACCTGAAGTCCGCCTCACGCACCGAAGTGCTC
GACTTCCTCACCAACGAACTCGGCATCTCCCGCTGA

[1] KS	34..410

[1] AT	558..864

[1] malonyl-CoA	737..741

[1] KR	1138..1314

[1] ACP	1419..1489

[1] KS	100..1230

[1] AT	1672..2592

[1] malonyl-CoA	2209..2223

[1] KR	3412..3942

[1] ACP	4255..4467

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like PS50075 ACP_DOMAIN G3DSA:1.10.1200.10 ACP_like PF00550 PP-binding IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR015083 Polyketide synthase, docking (Domain) PF08990 Docking IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR
SignalP	Eukaryota
TMHMM	No significant hit

Borre_00150 Borre_00170