Borre_00160 Borre_00160   Borre_00180 Borre_00180

Borre_00170 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction23,686 / 34,188 / + [in whole cluster]
23,686 / 34,188 / + [in contig]
Location23686..34188 [in whole cluster]
23686..34188 [in contig]
TypeCDS
Length10,503 bp (3,500 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)borrelidin polyketide synthase, type I
Gene
Gene (GenBank)borA3
EC number
Keyword
Note
Note (GenBank)
  • Module 2 (b-ketoacyl-ACP-synthase, acyltransferase, dehydratase, ketoreductase, acylcarrier protein) and Module 3 (b-ketoacyl-ACP-synthase, acyltransferase, dehydratase, ketoreductase, acylcarrier protein)
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
[14651102] Evidence from engineered gene fusions for the repeated use of a module in a modular polyketide synthase. (Chem Commun (Camb). , 2003)
[21472957] Stereoselectivity of isolated dehydratase domains of the borrelidin polyketide synthase: implications for cis double bond formation. (Chembiochem. , 2011)
[26501439] Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid. (ACS Synth Biol. , 2016)
comment
[PMID: 15112998](2004)
Borrelidin生合成gene clusterのクローニング。
borA3: PKS modules 2 and 3(KS-ATa-DH-KR-ACP-KS-ATp-DH-KR-ACP)

---
[PMID: 14651102](2003)
同じ人たちの先行する論文。
PKSの反復利用について報告している。

---
[PMID: 21472957](2011)
Borrelidinはtrans(module 2)とcis(module 3)と両方の二重結合を持つ。

ACP結合中間体の代理基質としてSNAC thioesterを用いて、BorDH2とBorDH3のin vitroでの変換能力をみている。

BorDH2の代理基質として3-hydroxybutanoic acid SNAC thioesterを使ったとき、BorDH2は(3R)-だけで触媒してtrans産物を導いた。BorDH3ではどちらもほとんど変換できなかった。

BorDH3の代理基質として3-hydroxy-2-methylpentanoic acid SNAC thioesterを使ったとき、BorDH2とBorDH3は両方とも(2R3R)-をtrans産物に変換した。

この結果を受け、cis二重結合ができるメカニズムについていくつかの可能性を挙げている。

---
[PMID: 26501439](2016)
borrelidin PKSのmodule 1を土台として、アジピン酸を産生できるキメラPKSを作製している。module 2のDH domainが導入されている。

close this sectionPKS/NRPS Module

2 malonyl-CoA
3 methylmalonyl-CoA
KS34..410
AT560..866
DH913..1076
KR1356..1529
ACP1628..1698
KS1724..2100
AT2245..2555
DH2603..2760
KR3062..3242
ACP3336..3410

close this sectionSequence

selected fasta
>polyketide synthase [borrelidin polyketide synthase, type I]
MPNDEELLDYLKRTASNLQEARQRVHELEESEREPIAIVGMSCRLPGGVNSPEEFWSLLE
AGTDAVSEFPRDRGWDVERLYDPDPDAPGKSYVREGGFLDGAGRFDPAFFGISPREAVVM
DPQQRLLLECSWEAIERSRIDPKTLHGSRAGVFVGSNGQDYGTLLLRADDRSHAYLATGA
SASVLSGRISYTLGLEGPAVTISTACSSSLVALHLAARALRAGECELALAGGVTVMPTTR
LFEVFSRQRGLAGDGRCKAFAAGADGTGWGEGVGVLVLERLSVARRNGHRVLAVVRGSAV
NQDGASNGLTAPNGPSQQRVIRAALASARLAPEDVDAVEAHGTGTSLGDPIEAQALLATY
GRGRDAERPLWLGSVKSNIGHAQAAAGVAGVIKMVKAMQAGTLPRTLHVDEPSGEVDWDS
GAVRLLTEARDWPSEEGRLRRAGVSSFGISGTNAHVILEEPPAEDAVPEPEAGDVVPWVL
SARSAEALREQAARLASVAGGLNVVDVGWSLASTRAAFEHRAVVVGREREELLAGLFAVA
AGRPAANVVTGPVSSGRPAFVFAGQGSQRLGMGRGLYERFPVFAEAFDEVCGRVGPGVRE
VVFGSDAGELDRTVWAQAGLFALEVALFRLLESWGVRPGCLIGHSVGELSAACVAGLWSL
EDACRVVAARARLMQALPAGGVMVAVRAEAGELAGFLGEDVVIASVNAPGQVVIAGPEGG
VERVVAACGARSRRLAVSHAFHSPLVEPMLGEFRRVVESVAFGVPSLRVVSNVTGAWVDP
EEWGTPEYWVRQVREPVRFADGVATLLDAGVRTFVELGPAGTLTSMVSHCADATATSVTA
VPTLRPDHDESRTVLSAAASLYVQGHPVDWAPLFPRARTVDLPTYPFQHQHYWMMNTGSA
AEPAELGLGDARHPLLGSVVTVAGDDKVVFAGRLALRTHPWLADHTVLDAVLLPATAFLE
LAVRAGEEVSCPVVHDLTLHRPLVVPERGAVQVQMAVGAPEADGRREVRVYSRPDDDAEH
EWTLHAAGLLASAATAEPAVAAGAWPPPEAQAVDLDGFYAGLAEHGYHYGPLFQGVRAAW
RLGDDVLAEIVLPEAAGADAARYGMHPALLDAVLHAARLGAFRERSEEKYLPFAWEGVTL
RTRGATAVRARISRAGTDAIRLDVTDTADRPVLTAESLTLRPVSAGQLMAVPRDSLFRVD
WVSAPAANGPGLRLARAATVEAALAADADIVVVPCLDSEGPHQATYQALELLQRWLAADT
GTTTLALLTHRAVAVGDDVHDLHHAPLWGLVRTAQTEHPGCFRLVDSDDPDPTTDVLAAA
LATGEPQVAIRDGAVLAPRLTAASAPREPAEWDAEGTVLITGGSGALAGIVAQHLVARHG
VRRLVLASRSGRPAPGADLLDADVTAVSCDVSDRDAVAALLASVPDEHPLTAVVHTAGVL
DDGVLHALTTERIDTSFAAKVDGARHLHELTSHLDLTAFVLFSSASAVLGAAGQGNYAAA
NAYLDALAAHRRSNDLPAVSLAWGLWAEHEGMARGLGDAELTRISRIGVTALSAEDGMRL
FDAGCAGDQSQLVPMRVDTAALRARRDHLPAPMWSLVPERTRAARTQPAASLRDRLAELT
APERKRTVLNLVRNAVADTLGHNAADGVPPDQSLDAAGFDSLTAVEFRNRLSAVTDLRLP
ATLTYDHPTPAAIAEHILTRLTLLKETAAPAVGTAPVAAPTEDDAIVIVGMAGRFPGGVR
TPEGLWDLVHSGTDAISEWPTDRGWDVENLYDPDPDAVGKSYVRHGGFLHDVAGFDAGFF
GISPREALAMDPQQRLLLECSYEALERAGIDPATLRGSRSGVYAGVMYHEYASRLGATPA
GFEGTLGTGSSGSIASGRISYTFDLTGPAVTVDTACSTSLVGLHLAVQALRAGECELALA
GGVTVMHTPRPFVEFSRQRGLAADGRSKAFAASADGVAWAEGAGILVLERLSAARRNGHR
VLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRAALASAGLGPADVDVVEAHGTGTALGDP
IEAQALLATYGRGRDADRPLWLGSVKSNIGHTQAAAGVASVIKMVQAMQAGVLPRTLHVD
EPSGEVDWDSGAVRLLTEAREWPSGEGRVRRAGVSSFGISGTNAHVILEEPPAEDALPEP
EAGDVVPWVLSARSAEALREQAARLASVAGGLNVVDVGWSLASTRAAFEHRAVVVGGDRE
ELLGKLSSVSGVEVGVGVGAGGGVVLVFAGQGCQWVGMGRELLGSSLVFAESMRECAAAL
SPFVDFSVVDVLGSAGELGRVEVVQPALWAVMVSLARVWRSWGVPVAAVVGHSQGEIAAA
TVAGALSVGDAARVVALRSRLIAERLSGLGGMVSVALSRERVVSLIAGVPGVSVAAVNGS
SSTVVSGEAAGLERVLAACVSSGVRARRIDVDYASHSVQVELIREELLGVLDGIVPRSGE
IPFVSTVTGERIDTVELGAEYWYRNLRQTVEFQSVVEGLVAQGCRVFLESSPHPVLTVGI
EESADRVVALESLRRGEGGLRRLVDAAGEAWVRGVPIDWAGMLAGGRRVDLPTYPFQHQP
YWLDSPRHPAGDVTAVGLTEAGHAFVPAAVDLPDGQRVWTGRLSLPSYPWLADHQVLGQV
LLPGVVWVELALHAGHQAGCDSVDELTLQSPLVLGASDTVQVRVVVTETEEPGTRTVSMH
SRRDDGSWVTHAEGILGAGGPPPEPLPEWPPTGAMPLDVEGFYDELAAGGYHYGPQFRCL
RRAWRAGEDLVAEISLPEGTDVDAYGLHPGLFDAAVHSVACARTSAGAGDDGPRLPFAFS
DVRLFATGVTSLRVRIDPQNSSWQAWDESGLPVLTIGRLAGRPVDADQFAVRRAGHLFRV
ETRHEALAGPAPASWAVIGADPAGYAAALEATGAQVTTAADLAGLTSAPEAALFTLPGTK
DAGVTEEVPTAVREATAQVLEVLQDWLTDGRFDDARLVVVSREAEDGDLLHGTARGLLRA
AQAEHPDRITLVDLDAHPASLTALPGFALGPEPEVVVRAGDGRAPRLARAQAPTGAGSLG
TGTVLITGGTGTLGGLLARHLVETHGVTRLLLVSRRGPAADGADRLHAELTGHGAHVDIV
AADLGDRTSVAALLATVDADHPLSAVVHAAGALDDGVLGTRSADWLDPVLRPKADAAWHL
HELTAELPLTAFVMFSSAASVLGAAGQANYAAANGFLDALAAHRAARGLPGTSLAWGLWE
HRSELTRHTGSPSRSIAAVGALSTAEALAAFDAGLASGEPLAVPIRLESTSSEEVPPMLR
GLVRVRRRAATGTEPAASAGAAQEVRQLAELGADERQRRVQRIVLDTAAAVLGHDSHDAI
PLTRGFLELGFDSLTAVRLRNRLARRLGLRLPATVVFDHPSPAALAAHLVEHLVGTVDPT
AQAMEQLEALRRSVHAATPAGGLDRALVTQRLTALLDEMRHVDGPGGTEGPDGSGDDLEN
ATADEIYALIDNELGIGGTQ
selected fasta
>polyketide synthase [borrelidin polyketide synthase, type I]
ATGCCCAACGACGAAGAACTCCTCGACTACCTGAAGCGGACTGCCTCGAACCTCCAGGAG
GCGCGGCAGCGGGTGCACGAACTGGAGGAGAGCGAGCGCGAGCCGATCGCGATCGTGGGG
ATGAGCTGCCGTCTGCCCGGCGGGGTGAACAGCCCGGAAGAGTTCTGGTCGCTGCTGGAG
GCCGGGACGGACGCCGTCTCGGAGTTCCCGCGGGACCGTGGCTGGGACGTGGAGCGGCTG
TACGACCCGGACCCGGACGCCCCCGGCAAGTCGTACGTGCGGGAAGGCGGATTCCTCGAC
GGCGCGGGCCGGTTCGACCCCGCGTTCTTCGGTATCTCCCCGCGGGAGGCCGTGGTCATG
GATCCGCAGCAGCGGCTGCTGCTGGAGTGCTCGTGGGAGGCGATCGAGCGGTCGCGGATC
GACCCGAAGACCCTGCACGGCAGCCGCGCGGGCGTGTTCGTGGGCTCGAACGGCCAGGAC
TACGGGACGCTTCTCCTGCGTGCCGACGACCGCTCCCACGCCTACCTCGCCACGGGCGCC
TCCGCGAGCGTGCTCTCCGGCCGCATCTCCTACACGCTCGGACTGGAGGGCCCTGCGGTC
ACGATCAGTACGGCCTGCTCGTCCTCACTGGTCGCCCTCCACCTGGCGGCCCGCGCCCTG
CGGGCGGGGGAGTGCGAGCTGGCGCTCGCCGGCGGTGTGACGGTCATGCCGACGACCCGC
CTGTTCGAGGTCTTCTCCCGGCAGCGTGGCCTGGCCGGTGACGGCCGCTGCAAGGCCTTC
GCGGCCGGGGCCGACGGCACTGGCTGGGGCGAGGGCGTGGGCGTACTCGTCCTGGAGCGG
TTGTCGGTGGCGCGGCGGAACGGTCATCGGGTGTTGGCGGTGGTGCGGGGTTCGGCGGTG
AACCAGGACGGTGCGTCGAACGGTCTGACGGCGCCGAACGGTCCGTCGCAGCAGCGGGTG
ATCCGCGCGGCCTTGGCCAGTGCACGCCTGGCCCCCGAGGACGTGGACGCCGTAGAGGCA
CACGGCACGGGGACCTCCCTGGGCGACCCGATCGAGGCGCAGGCGTTGCTGGCGACGTAC
GGGCGGGGCCGGGACGCGGAGCGTCCGTTGTGGCTGGGGTCGGTGAAGTCGAACATCGGT
CACGCGCAGGCCGCTGCCGGTGTCGCCGGTGTCATCAAGATGGTCAAGGCGATGCAGGCG
GGCACGCTGCCCCGGACGCTGCATGTGGATGAGCCGTCGGGTGAGGTGGACTGGGACTCG
GGTGCGGTGCGGCTGCTGACCGAGGCGCGGGACTGGCCGTCGGAGGAAGGTCGTCTGCGG
CGGGCCGGTGTGTCGTCGTTCGGGATCTCCGGCACCAACGCGCACGTGATTCTCGAGGAG
CCGCCGGCGGAGGACGCGGTACCGGAGCCTGAAGCGGGTGATGTGGTGCCGTGGGTTCTT
TCGGCGCGGTCGGCTGAGGCGTTGCGGGAGCAGGCTGCCCGGCTGGCGTCGGTGGCTGGT
GGGTTGAACGTGGTGGATGTGGGCTGGTCGTTGGCTTCGACGCGTGCGGCGTTCGAGCAC
CGGGCCGTAGTGGTGGGGCGGGAGCGGGAAGAGCTGCTCGCGGGTCTGTTCGCTGTGGCT
GCGGGACGCCCGGCTGCGAACGTGGTGACGGGGCCCGTCAGCTCCGGTCGGCCCGCCTTT
GTTTTTGCTGGTCAGGGCAGTCAGCGGTTGGGGATGGGGCGGGGGTTGTATGAGCGGTTC
CCGGTGTTTGCCGAGGCGTTCGACGAGGTGTGTGGGCGGGTCGGTCCGGGGGTGCGGGAG
GTTGTTTTCGGTTCGGATGCGGGTGAGTTGGACCGGACGGTGTGGGCGCAGGCGGGGTTG
TTCGCGTTGGAGGTGGCGCTGTTTCGGTTGTTGGAGTCCTGGGGTGTGCGGCCGGGTTGT
CTGATCGGGCATTCGGTCGGTGAGTTGTCGGCGGCGTGTGTGGCGGGGTTGTGGTCGTTG
GAGGATGCGTGTCGGGTCGTGGCTGCCCGGGCGCGGTTGATGCAGGCGTTGCCGGCGGGT
GGGGTGATGGTCGCGGTTCGGGCCGAGGCGGGGGAGCTGGCCGGTTTCCTCGGTGAGGAC
GTGGTGATCGCGTCGGTGAACGCGCCGGGGCAGGTGGTGATCGCTGGTCCTGAGGGGGGT
GTGGAGCGTGTGGTGGCTGCTTGTGGGGCGCGGTCGCGTCGTCTGGCGGTCTCGCATGCT
TTTCATTCGCCTTTGGTGGAGCCGATGCTTGGGGAGTTCCGTCGGGTTGTGGAGTCGGTG
GCGTTCGGTGTGCCGTCGTTGCGGGTGGTTTCCAATGTCACGGGTGCGTGGGTGGATCCG
GAGGAGTGGGGGACGCCGGAGTACTGGGTGCGTCAGGTCCGTGAGCCGGTGCGTTTCGCC
GACGGGGTCGCCACGTTGCTCGACGCGGGTGTGAGGACGTTCGTCGAGCTGGGTCCCGCT
GGGACGCTCACTTCGATGGTCAGCCACTGCGCGGACGCCACCGCCACTTCGGTGACGGCT
GTACCTACCTTGCGCCCCGATCACGATGAGTCGCGGACCGTGTTGAGTGCCGCAGCGTCC
TTGTACGTCCAGGGTCACCCGGTCGACTGGGCCCCGCTGTTCCCGCGGGCCCGCACGGTG
GACCTGCCCACCTACCCCTTCCAGCACCAGCACTACTGGATGATGAACACCGGAAGTGCC
GCCGAGCCGGCGGAGCTGGGGCTCGGCGATGCCCGTCATCCGCTGCTCGGTTCCGTCGTC
ACCGTCGCGGGGGACGACAAGGTCGTCTTCGCCGGGCGGCTGGCGCTGCGCACACACCCC
TGGCTGGCCGACCACACCGTGCTCGACGCGGTCTTGCTGCCCGCTACGGCCTTCCTCGAA
CTGGCCGTGCGCGCCGGTGAGGAGGTGAGCTGTCCGGTCGTACACGACCTGACGCTGCAC
CGACCGCTGGTCGTACCCGAGCGGGGCGCCGTGCAGGTACAGATGGCTGTGGGCGCACCG
GAAGCCGATGGGCGACGTGAGGTCCGGGTGTACTCCCGCCCCGACGACGACGCGGAGCAC
GAGTGGACGCTGCACGCCGCTGGACTGCTGGCGTCGGCCGCCACGGCGGAGCCCGCCGTG
GCGGCCGGTGCCTGGCCGCCGCCGGAGGCGCAGGCCGTGGACCTCGACGGCTTCTACGCC
GGACTCGCCGAGCACGGCTACCACTACGGCCCGCTGTTCCAGGGCGTCCGGGCCGCGTGG
CGGCTGGGCGACGACGTTCTCGCCGAGATCGTGCTGCCCGAGGCGGCCGGCGCCGACGCC
GCCCGGTACGGCATGCATCCGGCCCTGCTCGACGCCGTCCTGCACGCGGCACGGCTGGGC
GCCTTCCGTGAGCGGTCGGAGGAGAAGTACCTGCCGTTCGCCTGGGAAGGCGTGACCCTG
CGTACCAGGGGAGCGACCGCCGTACGTGCTCGAATCTCCCGGGCCGGTACCGACGCCATC
CGGCTGGACGTCACCGACACCGCGGACCGGCCGGTCCTCACGGCCGAATCGCTCACGCTG
CGACCGGTCTCCGCCGGTCAGCTCATGGCCGTCCCGCGCGACTCACTGTTCCGGGTCGAC
TGGGTTTCCGCGCCCGCCGCGAACGGTCCCGGCCTGCGGCTGGCCCGTGCCGCCACCGTG
GAGGCGGCCCTCGCGGCGGACGCCGACATCGTGGTCGTGCCATGCCTCGACAGTGAGGGT
CCGCATCAGGCGACGTACCAGGCACTGGAGCTGCTACAGCGCTGGCTGGCCGCCGACACC
GGTACCACCACGCTCGCCCTGCTCACCCACCGTGCCGTGGCGGTCGGCGACGACGTCCAC
GACCTCCACCACGCGCCTCTGTGGGGCCTGGTCCGCACCGCCCAGACCGAACACCCCGGC
TGCTTCCGGCTCGTCGACTCGGACGACCCCGACCCGACGACGGACGTCCTGGCCGCGGCG
CTCGCCACCGGGGAACCCCAGGTCGCGATCCGTGACGGCGCCGTCCTGGCCCCGCGGCTG
ACCGCGGCCTCCGCGCCGCGGGAGCCGGCCGAGTGGGACGCCGAGGGAACAGTCCTCATC
ACCGGCGGATCGGGCGCCCTCGCAGGGATCGTGGCCCAGCACCTCGTCGCACGTCACGGC
GTACGCCGACTCGTCCTCGCGAGCCGCAGCGGCAGGCCCGCACCGGGGGCCGACCTGCTC
GACGCCGACGTCACGGCCGTGTCCTGCGACGTCTCCGACCGCGACGCCGTGGCCGCGCTG
CTCGCCTCCGTGCCGGACGAACACCCGCTCACCGCCGTCGTGCACACCGCAGGCGTACTG
GACGACGGCGTCCTGCACGCCCTCACGACCGAGCGCATCGACACCTCGTTCGCGGCGAAG
GTCGACGGCGCCCGTCATCTCCACGAACTCACCTCCCACCTGGATCTCACCGCGTTCGTG
CTGTTCTCCTCCGCGTCGGCCGTGCTGGGCGCCGCCGGACAGGGCAACTACGCCGCGGCC
AACGCCTACCTCGACGCGCTCGCCGCCCACCGTCGCAGCAACGACCTGCCCGCCGTGTCT
CTCGCGTGGGGGCTGTGGGCCGAGCACGAGGGCATGGCCCGCGGACTCGGTGACGCCGAG
CTGACGCGTATTTCCCGGATCGGCGTCACCGCGCTGAGCGCGGAGGACGGCATGCGGCTG
TTCGACGCCGGATGCGCCGGCGATCAGTCACAGCTCGTGCCGATGCGGGTGGACACCGCG
GCGCTGCGCGCACGGCGTGACCACCTTCCCGCACCGATGTGGAGCCTGGTCCCCGAGCGG
ACCCGAGCGGCACGTACACAGCCTGCCGCCTCGCTTCGGGACAGGCTCGCCGAACTGACC
GCCCCCGAACGCAAGCGCACGGTCCTCAACCTGGTGCGCAACGCGGTCGCCGACACACTC
GGCCACAACGCCGCCGACGGAGTACCGCCCGACCAGAGCCTCGACGCCGCCGGGTTCGAC
TCGCTCACCGCCGTCGAGTTCCGCAACCGGCTCTCCGCCGTCACCGACCTGCGCCTGCCC
GCCACCCTCACCTACGATCACCCCACCCCCGCGGCCATCGCCGAGCACATCCTGACCCGC
CTCACCCTGCTGAAGGAGACCGCCGCCCCGGCCGTCGGCACCGCCCCGGTTGCGGCGCCG
ACCGAAGACGATGCGATCGTCATCGTGGGCATGGCGGGCCGCTTCCCTGGCGGCGTGCGC
ACACCCGAAGGTCTTTGGGACCTCGTCCACTCCGGCACGGACGCCATCTCGGAGTGGCCC
ACCGACCGCGGCTGGGACGTGGAGAACCTCTACGACCCGGACCCCGACGCCGTCGGCAAG
TCCTACGTACGGCACGGCGGATTCCTGCACGACGTCGCCGGCTTCGACGCGGGCTTCTTC
GGGATCTCGCCGCGTGAGGCGCTGGCGATGGACCCGCAGCAGCGGCTCCTGCTGGAGTGC
TCGTACGAGGCCCTGGAGCGGGCGGGCATCGACCCGGCCACGCTCAGAGGCAGCCGGTCG
GGCGTGTACGCCGGAGTGATGTACCACGAGTACGCCTCCCGGCTGGGCGCCACGCCCGCA
GGCTTCGAAGGCACACTCGGCACCGGAAGCTCGGGCAGCATCGCCTCCGGGCGCATCTCC
TACACATTCGACCTCACCGGGCCCGCGGTCACCGTCGACACCGCATGTTCCACCTCCCTC
GTAGGCCTGCACCTGGCCGTGCAGGCTCTGCGGGCCGGTGAGTGCGAACTGGCCCTCGCC
GGCGGCGTCACCGTCATGCACACGCCGCGCCCCTTCGTCGAGTTCTCCCGCCAGCGCGGC
CTGGCCGCGGACGGCCGGAGCAAGGCCTTCGCGGCCTCCGCCGACGGGGTGGCCTGGGCC
GAAGGCGCCGGAATCCTCGTCCTGGAGCGGCTGTCGGCGGCGCGGCGGAACGGTCATCGG
GTGTTGGCGGTGGTGCGGGGTTCGGCGGTGAACCAGGACGGTGCGTCGAACGGTCTGACG
GCGCCGAACGGTCCGTCGCAGCAGCGGGTGATACGTGCGGCCTTGGCGAGTGCCGGGCTG
GGTCCGGCCGATGTGGATGTCGTCGAGGCCCACGGCACCGGCACGGCCCTCGGCGATCCG
ATCGAGGCGCAGGCGTTGCTGGCGACGTACGGGCGGGGGCGTGACGCGGATCGTCCGTTG
TGGCTGGGGTCGGTGAAGTCGAACATCGGTCACACGCAGGCGGCCGCGGGTGTGGCAAGC
GTGATCAAGATGGTGCAGGCGATGCAGGCGGGCGTGCTGCCGCGGACGCTGCATGTGGAC
GAGCCGTCGGGTGAGGTGGATTGGGACTCGGGTGCGGTGCGGCTGCTGACCGAGGCGCGC
GAGTGGCCGTCGGGGGAGGGGCGTGTGCGGCGGGCGGGTGTGTCGTCGTTCGGGATCTCC
GGGACGAACGCGCACGTGATCCTTGAGGAGCCGCCGGCGGAGGACGCGCTGCCGGAGCCT
GAAGCGGGTGATGTGGTGCCGTGGGTTCTTTCGGCGCGGTCGGCAGAGGCGTTGCGGGAG
CAGGCTGCCCGGCTGGCGTCGGTGGCTGGTGGGTTGAACGTGGTGGATGTGGGCTGGTCG
TTGGCTTCGACGCGTGCGGCGTTCGAGCACCGGGCCGTCGTCGTGGGAGGCGATCGGGAA
GAGCTCCTGGGGAAGCTTTCCTCGGTTTCGGGGGTCGAGGTCGGGGTCGGGGTCGGTGCC
GGTGGTGGTGTGGTGTTGGTGTTCGCCGGTCAGGGGTGTCAGTGGGTCGGTATGGGGCGG
GAGTTGCTGGGTTCCTCGCTGGTGTTCGCGGAGTCGATGCGGGAGTGCGCGGCGGCTCTG
TCGCCGTTTGTGGACTTTTCTGTGGTGGATGTTCTGGGTTCGGCTGGGGAGTTGGGTCGG
GTCGAGGTGGTTCAGCCTGCGTTGTGGGCGGTGATGGTGTCGCTGGCGCGGGTGTGGCGG
TCGTGGGGTGTTCCGGTTGCTGCGGTGGTGGGTCATTCGCAGGGTGAGATTGCCGCGGCG
ACGGTGGCGGGTGCGTTGAGTGTGGGTGATGCGGCGCGGGTGGTGGCGTTGCGGAGCCGT
TTGATCGCGGAGCGTCTGTCGGGGCTGGGTGGGATGGTTTCGGTGGCGTTGTCGCGTGAG
CGGGTGGTGTCGTTGATCGCGGGTGTGCCGGGTGTGTCGGTGGCGGCGGTGAACGGTTCT
TCGTCGACGGTGGTCTCGGGTGAGGCCGCGGGGCTGGAGAGGGTGCTGGCCGCGTGTGTG
TCGTCGGGGGTTCGGGCGCGTCGTATCGATGTGGATTACGCCTCGCATTCGGTGCAGGTG
GAGTTGATCCGTGAGGAGTTGTTGGGGGTTCTGGACGGGATCGTCCCGCGCTCGGGTGAG
ATTCCGTTCGTGTCCACGGTGACGGGTGAGCGGATCGACACTGTCGAGCTGGGGGCGGAG
TACTGGTACCGCAATCTCCGTCAGACAGTGGAATTCCAGTCGGTGGTGGAGGGTCTGGTC
GCTCAGGGGTGTCGGGTGTTCCTGGAGTCCAGTCCGCATCCGGTGTTGACGGTCGGCATC
GAGGAGTCCGCGGATCGGGTCGTGGCGTTGGAGTCGCTGCGTCGTGGCGAGGGTGGTCTG
CGGCGGTTGGTGGATGCGGCCGGTGAGGCGTGGGTGCGTGGGGTGCCGATCGACTGGGCG
GGGATGCTCGCCGGCGGCCGGCGGGTCGACCTGCCCACCTATCCCTTCCAACACCAGCCC
TACTGGCTCGACTCACCACGACACCCTGCCGGAGACGTGACCGCCGTCGGTCTCACAGAG
GCCGGTCACGCGTTCGTGCCGGCGGCGGTCGACCTGCCGGACGGGCAGCGGGTCTGGACG
GGACGACTGTCGCTTCCCTCCTACCCGTGGCTGGCCGATCATCAGGTGCTCGGGCAGGTG
CTGCTCCCCGGCGTGGTCTGGGTCGAACTCGCCCTGCACGCGGGGCACCAGGCCGGATGC
GACTCTGTCGATGAGCTCACCCTACAGTCGCCGCTCGTGCTCGGTGCGTCCGACACCGTA
CAGGTGAGGGTCGTCGTCACGGAGACCGAAGAGCCCGGCACCCGCACCGTGTCGATGCAC
TCGCGCCGTGACGACGGCAGCTGGGTGACTCACGCCGAGGGGATCCTCGGGGCGGGCGGG
CCGCCGCCGGAGCCGCTGCCGGAATGGCCGCCGACCGGCGCCATGCCCCTCGATGTCGAG
GGCTTCTACGACGAGCTCGCGGCGGGCGGCTACCACTACGGGCCTCAGTTCCGCTGCCTG
CGGCGCGCCTGGCGTGCCGGTGAGGATCTCGTCGCCGAGATCTCGCTGCCGGAGGGCACC
GACGTCGATGCGTACGGCCTGCACCCTGGACTCTTCGACGCGGCGGTGCACAGCGTGGCC
TGCGCCCGGACGAGCGCGGGGGCCGGCGATGACGGTCCCCGGCTGCCGTTCGCCTTCTCG
GACGTCCGGCTCTTCGCGACCGGGGTGACCTCGCTACGGGTCCGGATCGATCCGCAGAAC
TCCTCGTGGCAGGCGTGGGACGAATCCGGGCTGCCGGTCCTCACCATCGGGCGGCTCGCC
GGCCGGCCTGTCGACGCCGATCAGTTCGCCGTGCGGCGGGCGGGCCACCTCTTCCGCGTC
GAAACGCGGCACGAAGCCCTGGCCGGCCCGGCCCCCGCCTCCTGGGCGGTCATCGGAGCG
GACCCGGCCGGGTACGCCGCAGCCCTGGAGGCCACGGGCGCGCAGGTGACGACGGCTGCC
GACCTGGCCGGTCTCACATCGGCACCCGAAGCCGCCCTGTTCACGCTCCCCGGCACAAAG
GACGCGGGGGTCACCGAGGAGGTGCCGACCGCTGTCCGGGAGGCGACCGCTCAGGTGCTG
GAGGTGCTGCAGGACTGGCTCACCGACGGACGTTTCGACGATGCCCGACTGGTCGTCGTA
AGCCGCGAAGCGGAAGACGGCGATCTCCTCCACGGAACGGCGCGCGGACTGCTGCGCGCC
GCACAGGCCGAGCACCCGGACCGCATCACCCTTGTCGACCTCGATGCTCATCCCGCCTCG
CTCACGGCCCTTCCCGGTTTCGCCCTCGGTCCCGAACCGGAGGTCGTCGTACGCGCGGGA
GACGGCAGGGCACCGCGCCTGGCCCGGGCGCAGGCCCCCACCGGAGCGGGCTCACTGGGC
ACGGGCACGGTCCTGATCACCGGAGGCACGGGCACCCTCGGGGGACTGCTCGCCCGGCAC
CTGGTGGAGACGCACGGAGTCACCCGGCTGCTGCTGGTCAGCCGACGAGGACCGGCCGCC
GACGGCGCGGACCGGCTGCACGCCGAGCTCACCGGGCATGGCGCACACGTCGACATCGTG
GCGGCCGACCTCGGCGACCGCACGAGCGTGGCCGCGCTCCTCGCCACGGTCGACGCCGAC
CACCCCCTGTCGGCCGTCGTGCACGCCGCCGGAGCGCTGGACGACGGCGTGCTCGGCACC
CGGTCCGCCGACTGGCTCGACCCGGTCCTGCGCCCCAAGGCGGACGCCGCTTGGCACCTG
CACGAACTCACCGCCGAACTGCCTCTGACCGCCTTCGTCATGTTCTCCTCGGCCGCATCC
GTGCTCGGCGCGGCGGGACAGGCCAACTACGCCGCGGCCAACGGATTTCTGGACGCACTG
GCCGCCCATCGTGCCGCCCGGGGACTGCCCGGGACCTCGCTGGCCTGGGGGCTGTGGGAG
CACCGCAGCGAACTGACCCGGCACACGGGCTCCCCCTCCCGCAGCATCGCGGCCGTCGGC
GCTCTGTCCACCGCGGAGGCCCTTGCCGCCTTCGACGCCGGCCTGGCCTCCGGGGAGCCG
CTGGCAGTGCCGATCCGGCTGGAGTCGACATCCAGTGAGGAGGTACCGCCGATGCTGCGC
GGCCTGGTCCGCGTACGCCGCCGGGCCGCCACCGGCACGGAACCCGCGGCGAGCGCGGGC
GCCGCGCAGGAGGTCCGGCAGCTGGCCGAGTTGGGCGCCGACGAGCGACAGCGGCGCGTG
CAGCGGATCGTGCTCGACACCGCGGCGGCCGTCCTCGGCCATGACAGCCACGACGCCATC
CCCCTCACCCGGGGCTTCCTGGAGCTGGGGTTCGACTCCCTGACAGCGGTACGGCTGCGC
AACCGGCTCGCCCGCCGACTGGGGCTGCGCCTGCCGGCCACGGTGGTGTTCGACCACCCC
AGCCCGGCCGCCCTGGCCGCCCACCTGGTCGAGCATCTCGTGGGCACCGTCGACCCGACC
GCGCAGGCCATGGAGCAGCTGGAGGCTCTGCGCCGCAGCGTGCACGCAGCCACACCCGCC
GGTGGCCTGGACCGCGCCCTGGTGACCCAACGCCTGACGGCCCTGCTCGACGAAATGCGG
CACGTCGACGGCCCCGGCGGCACCGAAGGCCCCGACGGCTCCGGGGACGACCTGGAGAAC
GCGACAGCGGACGAGATCTACGCCCTCATCGACAACGAACTGGGCATCGGGGGTACGCAG
TGA
[2] KS34..410
[2] AT560..866
[2] malonyl-CoA739..743
[2] DH913..1076
[2] KR1356..1529
[2] ACP1628..1698
[3] KS1724..2100
[3] AT2245..2555
[3] methylmalonyl-CoA2433..2437
[3] DH2603..2760
[3] KR3062..3242
[3] ACP3336..3410
[2] KS100..1230
[2] AT1678..2598
[2] malonyl-CoA2215..2229
[2] DH2737..3228
[2] KR4066..4587
[2] ACP4882..5094
[3] KS5170..6300
[3] AT6733..7665
[3] methylmalonyl-CoA7297..7311
[3] DH7807..8280
[3] KR9184..9726
[3] ACP10006..10230

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[550-677] G3DSA:3.40.366.10 [739-857] G3DSA:3.40.366.10 [2243-2365] G3DSA:3.40.366.10 [2433-2546] G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[3368-3383] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1631-1697] 5.29999999999999e-11 PF00550 [3344-3409] 1.4e-13 PF00550
PF00550   PP-binding
[1621-1740] 4.90001027782405e-25 SSF47336 [3332-3427] 1.60000240695997e-22 SSF47336
SSF47336   ACP_like
[1626-1701] 4.30000000000003e-41 G3DSA:1.10.1200.10 [3338-3414] 4.30000000000003e-41 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1628-1698] PS50075 [3336-3410] PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
[1356-1528] 2.29999999999998e-56 PF08659 [3062-3240] 7.5e-59 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[34-283] 4.59999999999996e-98 PF00109 [1724-1974] 1.69999999999998e-92 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[292-410] 1.70000000000001e-48 PF02801 [1982-2100] 4.5e-49 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[560-866] 5.49999999999995e-62 PF00698 [2245-2555] 1.29999999999998e-86 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-27] 6.40000000000001e-09 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[557-849] 1.19999063421924e-74 SSF52151 [2243-2527] 5.20000089003336e-60 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[679-738] 1.09999909120787e-12 SSF55048 [2368-2432] 2.09999806691534e-15 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[36-296] G3DSA:3.40.47.10 [297-463] G3DSA:3.40.47.10 [1726-1986] G3DSA:3.40.47.10 [1987-2153] G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[26-408] 6.80004680300024e-107 SSF53901 [1716-2098] 7.6999848053369e-103 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1352-1545] 8.30000000000004e-62 G3DSA:3.40.50.720 [3062-3263] 3.29999999999997e-63 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[197-213] PS00606 [1887-1903] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[561-848] 1.09999184471405e-114 SM00827 [2247-2537] 8.99992502689852e-108 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1629-1701] 3.69999438558106e-28 SM00823 [3341-3413] 1.50000306971104e-31 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[913-1076] 2.39999798157263e-83 SM00826 [2603-2760] 5.89998809405682e-71 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[36-463] SM00825 [1726-2153] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1356-1529] 4.10001399699315e-61 SM00822 [3062-3242] 6.79998707392899e-55 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Borre_00160 Borre_00160   Borre_00180 Borre_00180
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