Borre_00240 : CDS information

Location

Organism	Streptomyces parvulus
Strain	Tü4055
Entry name	Borrelidin
Contig	AJ580915
Start / Stop / Direction	54,506 / 56,176 / + [in whole cluster] 54,506 / 56,176 / + [in contig]
Location	54506..56176 [in whole cluster] 54506..56176 [in contig]
Type	CDS
Length	1,671 bp (556 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	GH3 auxin-responsive promoter family protein
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)	borL
EC number
Keyword	trans-1,2-CPDA
Note
Note (GenBank)
Reference	ACC Q70HZ3 PmId [15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004) comment [PMID: 15112998](2004) Borrelidin生合成gene clusterのクローニング。 borL: starter unit biosynthesis closest similar to Putative auxin-regulated protein borL insertional inactivation mutantはborrelidin産生が消滅。 borrelidin starter unitであるtrans-1,2-CPDAを加えるとborrelidin産生は回復し、wildよりも多くなる。よって、BorLはtrans-1,2-CPDA生合成に必須である。 promoter下でborLを過剰発現すると、borrelidin生合成は4.3倍に増える。既知の細菌タンパクとの強い類似点は見られないが、S.coelicolorのIclR and GntR family putative regulatory bacterial proteinsと弱い相同性を示すのでtrans-1,2-CPDAの調節をしているのかもしれない。
Family/Domain	FD IPR004993 comment [IPR004993] GH3 auxin-responsive promoter (Family)

Sequence

>GH3 auxin-responsive promoter family protein [hypothetical protein]
MTGNTTSAAFLRRTQNALAMQRKICAQPEETAERVFSDILSVSRDTGFGREHGLAGVRTR
QEWRRAVPIRTYDELAPYVERQFSGERRVLTTDDPRAFLRTSGSTGRAKLVPTTDHWRRV
YRGPALYAQWGLYFEQIGTHRLTGDEVLDLSWEPGPIRHRLRGFPVYSITERPVSDDPDD
WNPPWRHARWFTRDAGAATMADLLYGKLLRLAAHDLRLIVSVNPSKIVLLAETLKENAER
LIQDLHDGHGTDRAARPDFLRRLTAAFDRTGGRPLLTDLWPGLRLLVCWNSASAALYGPW
LSRLATGVAALPFSTTGTEGIVTLPVDDHLSAGPLAVDQGHFEFVPWQDLDDGSPLPEDT
PTLGYDELELGADYRLVMSQANGLYRYDVGDVYRVVGAVGATPRLEFLGRAGFQSSFTGE
KLTESDVHTAVMRVLGSERTDHPHFSGIPVWDTPPHYLVAIEWADAHGTLNVQDTARRID
ATLQEVNVEYADKRRSGRLRPLQILPLVPGAFGQIAERRFRQGTAGAQIKHHWLQKDSAF
LDTLRDLDLVRARPGT

>GH3 auxin-responsive promoter family protein [hypothetical protein]
GTGACCGGCAACACCACATCCGCCGCCTTCCTGCGGCGGACACAGAACGCGCTCGCCATG
CAGCGCAAGATATGCGCCCAGCCCGAGGAGACCGCGGAGCGCGTGTTCTCCGACATCCTC
TCGGTGTCACGAGACACCGGCTTCGGCCGCGAACACGGCCTCGCCGGGGTCCGCACCCGC
CAGGAGTGGCGGCGTGCCGTGCCCATCCGCACCTACGACGAACTGGCCCCCTACGTCGAG
CGGCAGTTCTCCGGCGAACGCCGCGTGCTCACCACCGACGACCCCCGCGCCTTCCTGCGC
ACCTCGGGATCGACCGGCCGCGCGAAGCTGGTACCCACCACCGATCACTGGCGCCGTGTC
TACCGCGGACCGGCGCTGTACGCGCAGTGGGGGCTCTACTTCGAACAGATCGGCACGCAT
CGGCTCACCGGCGACGAGGTCCTCGACCTGTCCTGGGAGCCCGGCCCCATCCGGCACCGA
CTGCGCGGCTTCCCCGTCTACAGCATCACCGAGCGCCCCGTGTCGGACGACCCCGACGAC
TGGAACCCGCCGTGGCGTCACGCGAGGTGGTTCACCCGCGATGCCGGTGCCGCGACCATG
GCCGACCTGCTCTACGGCAAACTGCTGCGGCTGGCCGCCCACGACCTGAGACTGATCGTC
TCGGTGAACCCCTCCAAGATCGTCCTGCTCGCCGAGACACTGAAGGAGAACGCCGAACGC
CTGATCCAGGACCTGCACGACGGCCACGGCACGGACCGGGCAGCCCGCCCGGACTTCCTC
CGCCGCCTCACCGCCGCCTTCGACCGCACCGGAGGCCGTCCGCTGCTCACCGACCTGTGG
CCCGGCCTGCGTCTGCTCGTCTGCTGGAACTCCGCCTCCGCGGCGCTGTACGGGCCCTGG
CTGTCCCGGCTCGCGACCGGCGTGGCGGCACTGCCGTTCAGCACCACGGGCACCGAGGGA
ATCGTCACGCTGCCCGTCGACGACCACCTCTCGGCGGGGCCGCTCGCTGTCGACCAGGGG
CATTTCGAATTCGTTCCGTGGCAGGACCTGGACGACGGCAGCCCTCTGCCCGAGGACACC
CCCACCCTCGGCTATGACGAACTCGAACTCGGCGCCGACTACCGGCTCGTCATGAGCCAG
GCCAACGGGCTCTACCGCTACGACGTGGGCGACGTGTACCGCGTCGTCGGAGCGGTCGGC
GCCACGCCACGGCTGGAGTTTCTGGGACGCGCGGGATTCCAGTCCTCCTTCACCGGCGAG
AAGCTCACCGAATCCGATGTGCACACCGCCGTGATGCGGGTCCTCGGCAGCGAACGCACC
GACCACCCGCACTTCTCCGGCATCCCGGTCTGGGACACCCCGCCCCACTACCTCGTCGCC
ATCGAATGGGCTGACGCCCACGGCACGTTGAACGTGCAGGACACAGCCCGCCGCATCGAC
GCGACTCTCCAGGAAGTCAACGTGGAATACGCCGACAAGCGCCGCAGCGGACGACTGCGG
CCCCTGCAGATCCTGCCCCTGGTGCCCGGCGCTTTCGGCCAGATCGCCGAACGAAGGTTC
CGCCAGGGCACCGCGGGAGCCCAGATCAAACACCACTGGCTGCAGAAGGACTCGGCGTTC
CTCGACACGCTGCGCGACCTCGACCTCGTCCGCGCCCGCCCGGGGACGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004993 GH3 auxin-responsive promoter (Family) PF03321 GH3 IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING
SignalP	No significant hit
TMHMM	No significant hit

Borre_00230 Borre_00250