Borre_00220 Borre_00220   Borre_00240 Borre_00240

Borre_00230 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction53,474 / 54,466 / + [in whole cluster]
53,474 / 54,466 / + [in contig]
Location53474..54466 [in whole cluster]
53474..54466 [in contig]
TypeCDS
Length993 bp (330 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative oxidoreductase
Product (GenBank)putative oxidoreductase
Gene
Gene (GenBank)borK
EC number
Keyword
  • trans-1,2-CPDA
  • nitrile
Note
Note (GenBank)
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
[15186422] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: insights into nitrile formation. (Mol Microbiol. , 2004)
comment
[PMID: 15112998](2004)
Borrelidin生合成gene clusterのクローニング。
borK: unknown
closest similar to Alcohol dehydrogenase

BorKのinactivationでborrelidin産生はほとんどなくなる。
そのmutantへのtrans-1,2-CPDA添加でborrelidin産生は回復。
よって、BorKはtrans-1,2-CPDA生合成に必須。

BorK or BorMが、2-oxohept-3-ene-1,7-dioate→2-oxohepta-1,7-dioateへの還元を担うかもしれない。

---
[PMID: 15186422](2004)
BorKのinactivationでborrelidin産生減少。
Streptomyces albus J1074においてはborIとborJを共に発現したときに12-desnitrile-12-methyl-borrelidin→borrelidinへの変換が可能で、さらにborKが同時に発現されると、より効率的であった。
C12-nitrile部分の形成に関与か。
Family/Domain
FD
IPR013154, IPR013149
comment
[IPR013154] Alcohol dehydrogenase GroES-like (Domain)
[IPR013149] Alcohol dehydrogenase, C-terminal (Domain)

function; oxidoreductase activity

close this sectionSequence

selected fasta
>putative oxidoreductase [putative oxidoreductase]
MKGLMRAAVIRAWGGPERLTLDRVERPSPPPGWIAVRVEACALNHLDIHVRNGLPGVRLE
LPHVSGGDVVGVVEQATDEAGERLLGSRVLLDPMIGRGILGEHYWGGLAEYVVAPAHNAL
PVPDQDADPARYAALPISYGTAQRMLFSRARLRPGESVLLFGATGGVGVACAQLALRAGA
RIIACSGSPAKLARLRRLGVIDTIDTGTEDVRRRVRELTDGGADLVVDYQGKDTWPVSLR
SARAGGRIVTCGATTGYEATTDLRYVWSRQLDILGSNAWHRDDLHTLVDLVATDALEPVV
HADFPLSRAPEAVAELEERRAFGKVVIRTA
selected fasta
>putative oxidoreductase [putative oxidoreductase]
GTGAAAGGGCTGATGCGGGCGGCCGTCATCCGTGCCTGGGGCGGCCCCGAGCGGCTGACC
CTGGACCGGGTCGAACGGCCGTCACCCCCGCCCGGATGGATCGCCGTACGCGTCGAGGCC
TGCGCCCTGAACCACCTCGACATCCACGTGCGCAACGGGCTTCCGGGCGTACGGCTGGAA
CTCCCGCACGTCTCCGGCGGCGACGTCGTCGGCGTCGTCGAGCAGGCCACCGACGAGGCG
GGGGAGAGACTGCTCGGCAGCCGTGTGCTGCTCGACCCGATGATCGGGCGCGGCATCCTC
GGCGAGCACTACTGGGGCGGGCTCGCCGAGTACGTCGTCGCACCCGCCCACAACGCGCTC
CCCGTCCCCGATCAGGACGCGGACCCGGCACGCTACGCCGCACTGCCCATCTCCTACGGC
ACGGCCCAGCGCATGCTCTTCAGCCGCGCCCGGCTGCGTCCCGGCGAGAGCGTGCTGCTG
TTCGGCGCGACCGGCGGCGTCGGCGTCGCCTGCGCCCAGCTCGCCCTGCGTGCCGGGGCC
CGGATCATCGCCTGCTCCGGATCACCGGCCAAGCTCGCCCGGCTGCGCCGACTCGGCGTG
ATCGACACGATCGACACCGGCACCGAGGACGTACGGCGCAGGGTCCGCGAACTCACGGAC
GGCGGTGCCGACCTGGTCGTCGACTACCAGGGCAAGGACACCTGGCCCGTCTCCCTGCGC
TCGGCGCGCGCCGGCGGCCGCATCGTCACCTGCGGCGCGACCACCGGGTACGAGGCGACG
ACCGACCTGCGCTACGTGTGGTCGCGTCAGCTGGACATCCTCGGCTCCAACGCGTGGCAC
CGCGACGATCTGCACACGCTGGTCGACCTGGTGGCCACCGACGCCCTGGAACCGGTGGTG
CACGCCGACTTCCCACTCTCCCGCGCCCCCGAGGCGGTCGCCGAACTGGAGGAGCGCCGG
GCGTTCGGGAAGGTCGTGATCCGCACGGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR011032 GroES-like (Domain)
[5-162] 3.80000697093588e-39 SSF50129
SSF50129   GroES_like
IPR013149 Alcohol dehydrogenase, C-terminal (Domain)
[166-292] 6.40000000000001e-30 PF00107
PF00107   ADH_zinc_N
IPR013154 Alcohol dehydrogenase GroES-like (Domain)
[31-96] 9.7e-09 PF08240
PF08240   ADH_N
IPR016040 NAD(P)-binding domain (Domain)
[161-276] 1.2e-32 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Borre_00220 Borre_00220   Borre_00240 Borre_00240
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