Borre_00210 Borre_00210   Borre_00230 Borre_00230

Borre_00220 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction52,113 / 53,477 / + [in whole cluster]
52,113 / 53,477 / + [in contig]
Location52113..53477 [in whole cluster]
52113..53477 [in contig]
TypeCDS
Length1,365 bp (454 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative aminotransferase
Product (GenBank)putative aminotransferase
Gene
Gene (GenBank)borJ
EC number2.6.1.-
Keyword
  • nitrile
Note
Note (GenBank)
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
[15186422] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: insights into nitrile formation. (Mol Microbiol. , 2004)
comment
[PMID: 15112998](2004)
Borrelidin生合成gene clusterのクローニング。
borJ: nitrile biosynthesis

---
[PMID: 15186422](2004)
borJはaminotransferaseをコードする。
C12-nitrile部分形成に関与。

borJのinsertional inactivationでborrelidin非産生、C12でnitrile基→carboxyl基になった12-desnitrile-12-carboxyl-borrelidinを蓄積。この産物は、borrelidin starter unit合成不全であるS. parvulus mutant(borE-, borI+, borJ+)においてborrelidinに変換できないshunt metabolite。

また、Streptomyces albus J1074においてはborIとborJを共に発現したときに12-desnitrile-12-methyl-borrelidin→borrelidinへの変換が可能で、dehydrogenaseをコードすると信じられているborKが同時に発現されると、より効率的であった。
Related Reference
ACC
P53555
PmId
[15880481] Removing a bottleneck in the Bacillus subtilis biotin pathway: bioA utilizes lysine rather than S-adenosylmethionine as the amino donor in the KAPA-to-DAPA reaction. (Biotechnol Bioeng. , 2005)
comment
102nd(P53555) 39%, 9e-90
B.subtilis_bioK(bioA)
L-Lysine-8-amino-7-oxononanoate aminotransferase(EC 2.6.1.-)

L-lysine + 8-amino-7-oxononanoate = 2-Aminoadipate 6-semialdehyde + 7,8-diaminononanoate.
B.subtilisではamino donorとしてS-adenosylmethionineの代わりにL-lysineのみを使用する。

close this sectionSequence

selected fasta
>putative aminotransferase [putative aminotransferase]
MEKTDVDRLRTLDREHMWYPWTPMTEWMARDQLVVERAEGCWLIDADGKRYLDGRSSMGM
NLHGHGRSEIVEALVAQARKAGETTLYRVSHPAAVELAARLASMAPAGLQRVFFAESGST
AVETALKAAYAYWVAKGEPQRSTFVSMEGGYHGETLGTVSLRGTNGEQVDMIRKTYEPLL
FPSLSFHQPHCYRCPVGQSSDSDCGLECTDSLENLLTREKGRIAAVIVEPRVQALAGVIT
APEGHLAKVAEITRRHGVLLIVDEVLTGWARTGPTFSCEAEGVTPDLMTVGKALTGGYLP
LSATLATEEIFGAFRESVFLSGSTYSGYALGAAVALASLDLFEKEDVPARAKALADVLTT
ALEPFRALTHVGDVRQLGLIAGVELVADRETRAPYPPQERVVDRICTLARDNGVLVNAVP
GDVITMLPSPSMSPDDLRFLTGTLYTAVREVTEE
selected fasta
>putative aminotransferase [putative aminotransferase]
GTGGAGAAGACCGACGTCGACCGGCTGCGCACACTCGACCGAGAGCACATGTGGTACCCG
TGGACGCCGATGACCGAGTGGATGGCCCGTGATCAGCTCGTCGTCGAACGCGCCGAAGGC
TGCTGGCTGATCGACGCAGACGGTAAGCGCTACCTCGACGGCCGCTCGTCGATGGGCATG
AACCTGCACGGCCACGGCCGCAGCGAGATAGTCGAGGCCCTGGTCGCCCAGGCGCGCAAG
GCCGGTGAGACCACGCTCTACCGCGTCTCGCACCCGGCGGCGGTGGAACTCGCCGCCCGC
CTGGCATCGATGGCGCCGGCCGGGCTCCAGCGCGTCTTCTTCGCCGAGTCCGGATCGACC
GCGGTGGAGACGGCTCTCAAGGCTGCCTACGCCTACTGGGTCGCGAAGGGCGAACCGCAG
CGATCCACCTTCGTGTCCATGGAGGGCGGTTACCACGGCGAGACCCTAGGCACGGTCAGC
CTGCGCGGCACCAACGGCGAACAGGTCGACATGATCCGCAAGACCTACGAGCCACTGTTG
TTCCCCTCCCTCTCCTTCCACCAGCCCCACTGCTACCGGTGTCCCGTCGGCCAGTCGTCG
GACAGCGACTGCGGGCTGGAGTGCACCGATTCGCTGGAGAACCTCCTCACCCGGGAGAAG
GGCCGGATCGCCGCGGTCATCGTCGAGCCGCGGGTCCAGGCCCTCGCCGGAGTGATCACC
GCCCCGGAGGGACACCTCGCGAAGGTCGCGGAGATCACCCGCAGGCACGGAGTGCTCCTC
ATCGTCGACGAGGTCCTCACCGGCTGGGCCCGCACCGGCCCGACGTTCTCCTGCGAGGCC
GAGGGCGTCACACCGGATCTGATGACGGTGGGCAAGGCGCTGACCGGCGGATATCTGCCG
CTGTCGGCCACCTTGGCCACGGAGGAGATCTTCGGAGCCTTCCGTGAGAGCGTCTTCCTC
AGCGGCAGCACCTACTCCGGATACGCGCTCGGGGCGGCCGTCGCCCTGGCCAGCCTCGAC
CTGTTCGAGAAGGAGGACGTACCGGCCCGGGCCAAGGCGCTCGCCGACGTGCTCACCACC
GCACTGGAACCCTTCCGCGCGCTCACCCACGTCGGTGACGTCCGGCAGCTCGGCCTCATC
GCCGGCGTCGAGCTGGTGGCCGACCGGGAGACCCGCGCCCCCTACCCGCCCCAGGAGCGC
GTCGTCGATCGCATCTGCACCCTGGCCAGGGACAACGGCGTGCTGGTCAACGCGGTCCCC
GGGGACGTGATCACCATGCTGCCCTCACCGTCGATGAGCCCCGACGACCTGCGCTTCCTC
ACCGGCACCCTGTACACGGCCGTCCGAGAGGTGACCGAAGAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005814 Aminotransferase class-III (Family)
[35-386] 2.50000000000001e-93 PF00202
PF00202   Aminotran_3
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[69-345] 7.99999999999993e-86 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[7-67] 5.49999999999995e-42 G3DSA:3.90.1150.10 [347-453] 5.49999999999995e-42 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[5-451] 8.00002659346736e-123 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Borre_00210 Borre_00210   Borre_00230 Borre_00230
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