Crypto_00020 Crypto_00020   Crypto_00040 Crypto_00040

Crypto_00030 : CDS information

close this sectionLocation

Organism
StrainATCC 53789
Entry nameCryptophycin 1
Contig
Start / Stop / Direction29,220 / 35,051 / + [in whole cluster]
29,220 / 35,051 / + [in contig]
Location29220..35051 [in whole cluster]
29220..35051 [in contig]
TypeCDS
Length5,832 bp (1,943 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)CrpC
Gene
Gene (GenBank)crpC
EC number
Keyword
Note
Note (GenBank)
  • non-ribosomal peptide synthetase; C-A-OM-PCP-E
Reference
ACC
PmId
[17240975] Biosynthetic characterization and chemoenzymatic assembly of the cryptophycins. Potent anticancer agents from cyanobionts. (ACS Chem Biol. , 2006)
[17240971] Cryptophycin anticancer drugs revisited. (ACS Chem Biol. , 2006)
comment
[PMID: 17240975](2006)
comment in: [PMID: 17240971](2006)

cryptophycin生合成遺伝子を同定した論文。
Supplementary Table 1.
CrpC: NRPS

cryptophycin1のaglycon形成には2つのPKSと2つのNRPSが関与。
NRPS(crpC-D)は、unitB(3-chloro-O-methyl-D-tyrosine)、unitC(methyl-beta-alanine)、unitD(branch chain alpha-hydroxy acids)の形成を担う。

取り込み実験から、unit BはL-tyrosineに由来し、Crp NRPSはO-methyl-D- and -L-tyrosine（すなわちL-3-([4-O-CH3]-phenyl)-alanine)の取り込みを触媒することが示された。

crpCはNRPSであるmodule 4をコードする。
domain構成は、C-A-OM-PCP-E
OM=proposed O-metyltransferase domain
E=epimerase(L-tyrosine→D-tyrosineへの変換を触媒すると予測される)

close this sectionPKS/NRPS Module

4 L-tyrosine
C60..356
A555..947
OM990..1265
PCP1389..1459
E1472..1937

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [CrpC]
MNLIEFLQDISIKGWQVWSEGERLCYDAPQEESTALVLAQLKQYKTEILQLLRDRPDILN
VYPLSYGQRALWFLWQLAPESHAYNVSFVARICSTVDITAMQKAFEKLIERHPILRTNYP
KLGSESIQQVNNFQELNFLQIDASAWSEDELKGKVIESHQQYFDLERGPVMRVRWFTRSK
KEHVLLLTIHHIACDAWSLDMLIQELPQLYQVQWADFKTPLSPLKHSYQDYVRWQRNILQ
ETEGERLWNYWQQKLTGDLPALNLATSRQRPPIKTYNGASHHFKLSDKLTKQLKELALNS
GATLYMMLLATFQVFLYRYTGQEDILVGSPTSGRSQAKFASILGYFVDPVVMRANLSGNP
SFKDFLAQVRQTVLEALAHQDYPFALLVEKLQPHRDPSRSPIFQASFSLLQFQKSQDIQK
LFVNQIETYVDWGELKIKPYEIPQQEGQLDLGLEMVEGSSSVFGVFKYNTDLFDESTIER
MAGHFQNLLSAIVENPQQKVSESPLLSEVERHQLLVEWNDTAREYPSKCIHQLFEEQVER
TPDAVAVVFENQQLTYQQLNQKANQLAHHLLSLRVEPEVLVGIYVERSFEMVVGLLGILK
AGGAYVPLDPNYPQERLSYMLADSGVEVLLAQKSLLESLPSHTAQVVCLDSDWGVIEQHS
QENLDVGVCSDNLAYVIYTSGSTGVPKGVGIEHFSLCNLIQAQKNLFYLEPNSRVLQFAS
ISFDASVSEIFIALTSGAMLILAIASELIPGSDLKQILQERCVTHVTLPPSALAVLATDE
FPALGQIIVAGEACNLELANQWSVGRRLFNGYGPTESTIGAAVAQISHGSEKVTIGRPIA
NTQIYILDKHLEPVPISVSGELYIGGYGLARGYLNRPELTLEKFIPNPFNSRSKLYKTGD
LARYLPDGNIEFLGRLDNQVKIRGFRIELGEIESVLSTHPQVQQVAVTEREDTPGHKRLV
AYLVPLQSKEKDPQPQTGIELWPSVAEFYVYDELLYYAMTNDHRRNQSYQVAINQMVKDK
VVVEIGTGKDAIIARFCAEAGAKKVYAIERDEQTSKLASACVQELGLSEQIQIIHGDATT
ANLPEEVDVCVSEIVGPIGGSEGAAVIINNARRFLKSDGVMIPQRSVTQIIAVTLPDELL
NQPQFTKVSGYYTQKIFEQVGYPFDLRVCIKGLNQVNWLSNRGVFEDLDFSKLVSTESTH
QIKLTIEKSGRLDGFSVGLNLHTIEGECIDILENEHCWLPVYLPVFEPGIYVNEGDIIEA
VCTRTLCENNLNPDYTLRGRLLKKTGKEIEFEYISYHWKQLFKQNPFYQRLFAENNLEDY
TINKSKPERKVLSSNELRSYLKSKLPEYMLPSSFVILDTLPLTPNGKVDRKALSAPDGEV
SREDEYVAPRTEIEQILTNIWQELLLKEQVSIHDNFFEIGGDSILGIQVVSRAKNLGIQI
TPKQIFQNQTIAKLALVANTTVTVSANQGIVTGVAPLTPIQQWFFAQNSQEAHHYNQSVL
LQIPNHLQTELIETALKKLLEHHDALRLRFTSVASEYKQINHGFDDPVAFTVVDLSSTPV
IEQPQALSQIATEYQASLNLSEGPLMQVVMFNLGSEVDARLLIIIHHLAVDGVSWRILLS
DLETIYQQLIAQQSIQLNAKTTAFIDWAEKLKNYAQSEKIKQELDYWLNQPWSETTPLPL
DSAHTQAEKTVDSAINYRVKLSPEETRALLGSVNSAYNTQINDILLSALVVSLAEWTGDS
KVLIDLEGHGREELFSDVDLSRTIGWFTSLFPVLLRLPDDKQPAEVIKSIKEQLREIPHR
GIGFGILRYLCEDTTVTQKLQTIPTPEISFNYLGQFDQIQSETGWKFAPESTGDNHSSKQ
TRHHLLEINSLVVEGELQIDWTYSSNFHTHDTVKNLTQSYIQAIKSIIEHCQSENGFGYT
ASDFPDAQLNQLELDELLKNLGK
selected fasta
>non-ribosomal peptide synthetase [CrpC]
ATGAATTTAATCGAATTTTTACAAGATATTTCAATAAAAGGTTGGCAAGTGTGGAGTGAG
GGCGAACGGCTTTGTTACGATGCTCCTCAAGAAGAATCAACTGCTTTAGTATTAGCTCAA
CTGAAACAATACAAAACCGAAATATTACAATTGCTGCGCGATCGCCCAGATATTTTAAAC
GTCTATCCCCTTTCTTACGGTCAACGGGCACTTTGGTTTTTGTGGCAATTAGCACCAGAA
AGTCATGCTTATAACGTATCATTTGTCGCTCGTATTTGCTCAACAGTAGATATTACAGCA
ATGCAAAAGGCATTTGAGAAACTAATAGAACGGCATCCGATATTGCGTACAAATTATCCC
AAACTCGGATCTGAGTCAATCCAACAGGTAAATAATTTTCAGGAATTAAATTTCTTGCAA
ATTGATGCTTCTGCTTGGAGTGAAGATGAACTGAAAGGGAAAGTGATTGAGAGCCATCAA
CAATATTTTGACCTCGAACGAGGGCCTGTTATGCGAGTTCGGTGGTTTACTCGTTCCAAG
AAAGAACATGTCCTGTTGCTAACCATACACCACATTGCTTGCGATGCCTGGTCTTTAGAT
ATGTTAATACAGGAGTTGCCACAACTCTACCAAGTACAATGGGCTGATTTTAAGACACCC
CTTTCTCCTCTAAAGCATTCTTACCAAGATTACGTTCGTTGGCAGAGGAATATTTTGCAG
GAGACTGAAGGGGAAAGACTCTGGAACTACTGGCAGCAAAAACTGACAGGCGATTTGCCA
GCACTAAACCTAGCAACCTCAAGACAGCGACCACCGATAAAAACTTATAATGGTGCTTCC
CATCACTTCAAATTATCTGACAAGCTCACTAAGCAACTCAAGGAGCTGGCTTTAAACTCG
GGAGCAACACTTTACATGATGCTCTTAGCAACTTTTCAGGTGTTTTTGTATCGTTACACA
GGCCAGGAGGATATTTTAGTCGGTTCTCCCACCTCAGGTAGGAGTCAAGCTAAGTTTGCT
TCGATCTTGGGCTACTTTGTTGATCCTGTTGTTATGCGAGCAAATTTATCGGGAAATCCC
AGTTTCAAAGATTTCCTCGCCCAAGTACGCCAAACCGTATTGGAAGCACTTGCTCATCAA
GATTACCCATTTGCTCTATTGGTAGAAAAGCTACAGCCACACCGCGACCCCAGTCGTTCG
CCGATTTTTCAGGCTTCTTTTTCTCTACTGCAGTTCCAAAAATCTCAAGATATACAGAAG
TTGTTTGTAAATCAAATAGAAACCTATGTTGATTGGGGAGAATTGAAGATAAAACCTTAT
GAAATACCTCAACAGGAAGGTCAGTTAGATTTAGGTTTAGAAATGGTGGAGGGGAGTTCA
TCTGTTTTTGGGGTTTTTAAGTACAACACTGACTTGTTTGATGAGTCAACCATTGAGCGC
ATGGCTGGTCATTTCCAGAATTTATTGTCAGCGATTGTGGAAAATCCTCAACAAAAGGTA
AGTGAATCACCCCTATTGAGTGAAGTAGAACGGCATCAGTTGTTGGTGGAGTGGAATGAT
ACAGCAAGGGAATATCCCAGTAAATGTATCCATCAATTGTTTGAAGAGCAGGTAGAGAGA
ACACCGGATGCAGTCGCGGTGGTATTTGAGAATCAGCAGTTAACCTACCAACAATTAAAT
CAAAAAGCCAACCAACTAGCACATCACCTGCTTTCCTTGCGAGTCGAACCAGAGGTGCTG
GTAGGGATTTACGTGGAGCGTTCTTTTGAAATGGTGGTAGGGCTCTTGGGGATACTCAAG
GCTGGTGGGGCTTATGTACCCCTTGACCCCAATTATCCCCAAGAACGGTTGAGTTATATG
TTGGCGGATTCAGGTGTTGAGGTGTTGTTGGCTCAAAAGTCCCTACTGGAATCTTTGCCG
TCACATACTGCACAGGTGGTTTGTTTGGATAGTGATTGGGGAGTGATTGAGCAACACAGT
CAGGAGAATCTTGATGTTGGGGTATGTTCAGATAATTTGGCTTATGTGATTTATACTTCT
GGTTCTACTGGAGTTCCCAAGGGGGTTGGGATTGAACATTTTTCTTTGTGCAACCTTATT
CAAGCACAGAAAAATTTATTTTATCTAGAACCAAATAGTCGTGTTCTTCAATTTGCTTCT
ATAAGTTTTGATGCTTCAGTTTCGGAAATATTTATCGCTTTGACTTCTGGAGCAATGCTA
ATTTTGGCTATAGCTTCTGAGTTAATCCCGGGTTCGGATTTAAAGCAAATTTTACAAGAA
AGATGTGTAACTCATGTTACGTTACCTCCCTCTGCCCTGGCAGTACTAGCTACTGATGAA
TTTCCAGCCTTGGGTCAGATAATTGTGGCAGGGGAAGCTTGTAATTTAGAATTGGCCAAT
CAGTGGTCTGTTGGTCGTCGTTTGTTTAATGGTTATGGGCCTACTGAGTCTACTATTGGT
GCTGCAGTGGCTCAAATCAGCCATGGTAGCGAGAAAGTTACTATCGGTCGCCCCATTGCA
AACACCCAAATCTATATCTTAGATAAGCACTTAGAACCAGTACCCATAAGTGTATCCGGA
GAATTATACATCGGAGGTTATGGCTTAGCCAGAGGTTACCTCAACCGACCGGAATTAACT
TTAGAGAAATTCATCCCTAACCCCTTCAATAGCAGATCAAAACTATATAAAACTGGAGAT
TTAGCTCGATACTTACCAGATGGTAATATTGAGTTTCTTGGTCGTCTTGATAACCAGGTG
AAAATACGTGGTTTCCGCATAGAACTAGGAGAAATAGAATCAGTTCTGAGTACCCATCCT
CAAGTACAGCAAGTCGCAGTCACCGAAAGGGAAGATACTCCGGGTCACAAACGGTTGGTA
GCATATTTAGTTCCTCTTCAGTCAAAAGAAAAAGATCCCCAGCCCCAGACAGGAATTGAA
CTTTGGCCCTCAGTAGCAGAATTCTACGTTTATGATGAGCTCTTGTACTATGCGATGACC
AATGATCATCGTCGAAACCAGAGTTACCAAGTCGCAATTAATCAAATGGTTAAAGATAAA
GTAGTTGTTGAAATTGGCACGGGCAAGGATGCAATTATAGCCAGATTTTGTGCAGAAGCA
GGTGCTAAGAAAGTCTACGCAATTGAGAGAGACGAGCAAACCAGTAAGTTAGCTTCAGCT
TGCGTGCAAGAGTTGGGGTTATCAGAACAAATTCAAATCATACATGGAGACGCTACTACA
GCCAACTTACCAGAAGAAGTTGATGTATGTGTTTCTGAAATTGTGGGACCCATTGGTGGA
TCTGAAGGAGCAGCAGTAATTATCAACAACGCCAGAAGATTTCTCAAATCAGATGGTGTA
ATGATTCCCCAAAGAAGTGTGACTCAAATTATTGCAGTAACTCTTCCTGATGAATTACTA
AATCAACCCCAATTTACAAAAGTTTCAGGTTATTATACCCAGAAGATATTTGAGCAAGTT
GGATATCCTTTTGATTTACGAGTATGTATTAAAGGATTAAATCAAGTAAACTGGTTGTCC
AATCGAGGAGTTTTTGAGGATTTAGACTTTAGCAAGCTTGTTAGCACAGAATCTACTCAC
CAAATTAAATTAACTATTGAAAAATCAGGAAGATTAGATGGTTTTTCAGTGGGATTAAAC
TTACACACAATTGAAGGAGAATGTATAGATATTTTAGAAAATGAACATTGCTGGTTACCA
GTTTATCTTCCAGTGTTTGAACCAGGAATTTATGTAAATGAAGGAGATATCATCGAAGCT
GTTTGTACGAGAACGCTTTGTGAAAATAATTTGAATCCAGATTATACTCTTCGAGGTCGT
CTTTTAAAAAAAACTGGAAAAGAGATTGAGTTTGAATACATCTCATATCATTGGAAACAA
TTGTTTAAGCAAAACCCTTTTTATCAACGATTGTTTGCTGAAAATAATCTAGAAGATTAC
ACAATTAATAAGTCAAAGCCTGAACGTAAGGTACTGAGTAGCAATGAATTGCGCTCCTAC
CTAAAGTCTAAATTGCCAGAATATATGCTCCCCTCTAGCTTTGTCATCTTAGACACCCTA
CCGTTGACACCCAACGGTAAAGTAGACCGCAAAGCCCTAAGTGCACCTGATGGGGAAGTT
AGCCGAGAGGATGAATATGTCGCACCACGCACAGAAATAGAACAAATCTTAACCAACATT
TGGCAAGAACTGCTCCTTAAAGAACAAGTCAGCATCCATGACAACTTCTTTGAAATTGGT
GGCGACTCCATCCTTGGTATTCAAGTAGTTTCTCGTGCCAAAAACTTAGGAATACAAATC
ACTCCCAAACAAATATTCCAAAATCAAACCATCGCCAAACTAGCATTAGTAGCCAATACA
ACAGTTACTGTCAGTGCTAACCAAGGTATAGTTACTGGAGTTGCACCCCTAACACCAATT
CAACAGTGGTTCTTTGCACAAAATAGCCAAGAAGCACACCATTACAACCAATCAGTTTTA
TTGCAGATTCCCAATCATCTGCAAACTGAATTAATCGAAACAGCCTTGAAAAAATTATTA
GAGCATCACGATGCTCTGCGTTTACGATTCACATCAGTTGCATCTGAGTACAAACAAATA
AACCATGGCTTTGATGATCCCGTAGCATTTACTGTAGTTGATTTATCATCAACTCCTGTC
ATTGAACAACCACAAGCTTTATCACAGATCGCCACGGAATATCAAGCAAGTTTAAACCTC
TCAGAGGGACCTTTAATGCAAGTGGTGATGTTTAACTTAGGTAGTGAAGTTGATGCCCGT
TTACTGATTATTATTCATCACCTAGCAGTAGATGGTGTGAGTTGGCGAATTTTACTATCA
GACTTAGAAACAATCTATCAACAACTAATCGCTCAACAATCAATACAGCTAAATGCGAAA
ACAACAGCATTTATTGATTGGGCAGAGAAATTGAAAAATTATGCACAATCAGAAAAAATC
AAACAAGAGTTAGACTATTGGCTCAACCAACCTTGGTCAGAAACAACACCACTACCATTA
GATTCTGCTCACACTCAAGCAGAAAAAACAGTTGATAGTGCGATTAATTATAGAGTGAAA
TTGAGTCCAGAAGAAACCCGCGCTTTGCTGGGGTCAGTAAACTCAGCTTATAACACACAA
ATTAACGATATCCTCCTCAGTGCATTAGTAGTTTCCTTGGCAGAGTGGACGGGAGATTCA
AAAGTACTAATTGACCTAGAAGGACATGGCAGAGAAGAACTATTTTCAGATGTAGACTTA
TCAAGAACAATAGGTTGGTTTACCAGTTTATTCCCAGTATTATTGCGATTACCAGACGAT
AAACAACCAGCAGAAGTTATCAAGTCAATTAAAGAACAATTACGAGAGATTCCCCATCGT
GGTATTGGCTTTGGTATATTGCGTTACTTGTGTGAAGATACTACTGTAACCCAAAAACTA
CAGACAATTCCTACTCCAGAAATTAGTTTTAACTACCTAGGACAATTTGACCAAATACAA
TCGGAAACGGGTTGGAAATTTGCGCCAGAATCTACTGGAGATAATCATAGTTCAAAGCAA
ACTCGTCACCATCTATTAGAGATTAATAGTCTGGTGGTAGAAGGTGAATTACAAATTGAT
TGGACTTATAGTAGTAATTTTCATACTCATGATACAGTAAAAAATTTGACACAAAGCTAT
ATTCAAGCAATTAAGTCAATAATAGAACATTGCCAGTCAGAAAATGGTTTTGGATATACA
GCTAGTGATTTCCCAGATGCACAGTTAAATCAATTAGAACTTGATGAGTTATTAAAAAAC
TTGGGCAAATAG
[4] C60..356
[4] A555..947
[4] L-tyrosine724..820
[4] OM990..1265
[4] PCP1389..1459
[4] E1472..1937
[4] C178..1068
[4] A1663..2841
[4] L-tyrosine2170..2460
[4] OM2968..3795
[4] PCP4165..4377
[4] E4414..5811

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[555-947] 6.20000000000009e-119 PF00501
PF00501   AMP-binding
IPR001242 Condensation domain (Domain)
[60-356] 3.70000000000004e-72 PF00668 [1472-1775] 1.69999999999998e-69 PF00668
PF00668   Condensation
IPR006162 Phosphopantetheine attachment site (PTM)
[1418-1433] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1397-1455] 7.59999999999999e-10 PF00550
PF00550   PP-binding
[1384-1457] 2.6e-25 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1386-1455] 1.29999924468179e-16 SSF47336
SSF47336   ACP_like
[1389-1459] PS50075
PS50075   ACP_DOMAIN
IPR010060 Non-ribosomal peptide synthase (Domain)
[1784-1937] 8.60000000000004e-49 TIGR01720
TIGR01720   NRPS-para261
IPR010071 Amino acid adenylation domain (Domain)
[555-947] TIGR01733
TIGR01733   AA-adenyl-dom
IPR020845 AMP-binding, conserved site (Conserved_site)
[676-687] PS00455
PS00455   AMP_BINDING
IPR025799 Protein arginine N-methyltransferase (Family)
[990-1265] 1.5e-11 PF05185
PF05185   PRMT5
SignalP No significant hit
TMHMM No significant hit
Crypto_00020 Crypto_00020   Crypto_00040 Crypto_00040
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