Crypto_00050 : CDS information

Location

Organism	Nostoc sp.
Strain	ATCC 53789
Entry name	Cryptophycin 1
Contig	EF159954
Start / Stop / Direction	45,180 / 46,532 / + [in whole cluster] 45,180 / 46,532 / + [in contig]
Location	45180..46532 [in whole cluster] 45180..46532 [in contig]
Type	CDS
Length	1,353 bp (450 aa)

Annotation

Category	3.3 modification reduction
Product	epoxidase cytochrome P450
Product (GenBank)	CrpE
Gene
Gene (GenBank)	crpE
EC number
Keyword
Note
Note (GenBank)	CYP450 responsible for epoxidation of cryptophycins
Reference	ACC A4D937 PmId [17240975] Biosynthetic characterization and chemoenzymatic assembly of the cryptophycins. Potent anticancer agents from cyanobionts. (ACS Chem Biol. , 2006) [17240971] Cryptophycin anticancer drugs revisited. (ACS Chem Biol. , 2006) [18366166] Analysis of the cryptophycin P450 epoxidase reveals substrate tolerance and cooperativity. (J Am Chem Soc. , 2008) comment [PMID: 17240975](2006) comment in: [PMID: 17240971](2006) cryptophycin生合成遺伝子を同定した論文。 Supplementary Table 1. CrpE: cytochrome P450 epoxidase crpEはP450 oxygenaseをコードし、いくつかのcryptophycins(1, 2, 52)で見られるepoxideの形成を担う。 --- [PMID: 18366166](2008) chemoenzymatic synthesisによって生み出された構造的に関連したcryptophycin analoguesと共に調査され、CrpEは特異的にcyclic cryptophycin analoguesのC2' and C3' の間にbeta-epoxideを設置することがわかった。kcat/Km値測定、binding analysisもしている。

Sequence

>epoxidase [CrpE]
MINTAKSSLLPGPTTPSWWNLLQWLNNPCEFLEECRARYGDTFTFKAIGFEPLVLISNPK
DIKEIFDKHKYFDSGKAKANDLAGFFLGNNSVTLLDGSSHKRQRKLLMPAFHGQNISNYG
ELICHATKQVTSNWQPGQRLIIYKEVKEITLRAMLTVLLGSDKTERYQQLKLIVNQIVST
ITNPFASSSLFFNVFRRDWGSWSAWGNLLRCQRQIANIISAEIKERRENCNNYNNDILSM
LMAARDENGGKMTDEELQDELMTLIFSGYETTSAAITWAYYWIHYLPEIRAKLLQELDEL
GDNPDPTEISKLPYLNAVCAETLRIYPVGLTTFPRIVKSPIEIGGHQFEVGTCLYPCIYL
IHHREELYPNSKQFKPERFLDNKFLNYEYFPFGGGNRTCIGMAFAQFKMKLVLANILRNW
QLELVGKPPLKPVRDIFSIYPQGGLKMVVL

>epoxidase [CrpE]
ATGATTAATACTGCTAAATCCTCATTACTTCCTGGTCCCACTACACCATCTTGGTGGAAC
TTATTGCAATGGCTTAATAATCCTTGTGAATTTTTGGAAGAGTGTCGAGCACGCTATGGA
GACACTTTTACCTTCAAAGCTATTGGTTTTGAACCTTTAGTACTTATTAGTAATCCTAAG
GATATAAAAGAAATTTTTGATAAACACAAGTATTTTGACAGTGGAAAAGCTAAAGCTAAC
GATTTAGCAGGATTTTTTTTAGGCAACAATTCCGTCACCTTGCTTGATGGAAGTAGTCAT
AAACGACAGCGTAAACTACTGATGCCTGCTTTTCATGGTCAAAATATATCTAACTATGGA
GAACTAATATGCCATGCAACGAAGCAGGTTACTTCTAATTGGCAACCTGGTCAAAGATTG
ATTATTTACAAGGAAGTCAAAGAAATTACGCTGCGAGCGATGTTAACGGTTTTACTGGGT
TCAGATAAAACGGAACGTTATCAACAACTCAAATTGATAGTTAATCAAATAGTATCCACT
ATAACTAATCCCTTTGCTTCTAGCTCTCTTTTCTTCAATGTGTTTAGAAGAGACTGGGGT
TCTTGGAGTGCCTGGGGTAATCTTTTACGTTGCCAACGTCAGATTGCAAATATCATTTCT
GCAGAAATCAAAGAACGTAGAGAAAATTGTAACAATTACAACAATGATATCCTCAGTATG
CTGATGGCAGCACGAGATGAAAATGGAGGAAAAATGACAGATGAGGAGTTGCAAGATGAG
TTAATGACACTTATCTTTTCTGGATATGAAACTACATCTGCAGCAATAACATGGGCATAT
TATTGGATTCATTACTTACCAGAGATAAGAGCCAAGTTATTGCAAGAATTAGATGAGTTA
GGAGATAATCCAGACCCAACGGAAATAAGCAAATTACCTTATCTCAATGCAGTTTGTGCT
GAAACCTTGAGAATATATCCAGTTGGTCTAACTACTTTTCCTCGAATTGTAAAATCGCCA
ATAGAAATTGGAGGTCATCAATTTGAGGTAGGAACTTGTCTTTATCCATGTATTTATCTA
ATTCACCACCGGGAAGAACTATATCCTAACTCTAAACAGTTTAAGCCAGAACGTTTTCTA
GATAATAAATTTTTAAATTATGAGTATTTCCCTTTCGGTGGCGGTAACCGAACTTGCATT
GGTATGGCATTTGCTCAGTTTAAAATGAAGTTAGTATTGGCTAATATTTTGCGGAATTGG
CAATTGGAATTGGTAGGCAAACCTCCTTTAAAACCAGTACGAGATATTTTCTCAATTTAT
CCTCAAGGTGGATTAAAAATGGTTGTATTGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 PR00385 P450 PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 IPR002401 Cytochrome P450, E-class, group I (Family) PR00463 EP450I IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	Eukaryota
TMHMM	No significant hit

Crypto_00040 Crypto_00060