Epoth_00070 Epoth_00070   Epoth_00090 Epoth_00090

Epoth_00080 : CDS information

close this sectionLocation

Organism
StrainSo ce90
Entry nameEpothilone
Contig
Start / Stop / Direction16,251 / 21,749 / + [in whole cluster]
16,251 / 21,749 / + [in contig]
Location16251..21749 [in whole cluster]
16251..21749 [in contig]
TypeCDS
Length5,499 bp (1,832 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase
Gene
Gene (GenBank)epoB
EC number
Keyword
Note
Note (GenBank)
  • EPOS B
Reference
ACC
PmId
[10662695] The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90. (Chem Biol. , 2000)
[11217802] Studies on the biosynthesis of epothilones: the biosynthetic origin of the carbon skeleton. (J Antibiot (Tokyo). , 2000)
comment
[PMID: 10662695](2000)
生合成cluster報告論文。
PKS(9modules)のmodule 1をコード。

Mod 1; KS, ATp, DH, KR, ACP

--
[PMID:11217802](2000)UniProt登録外
取り込み実験によりepothilone骨格の由来を調査。
Related Reference
ACC
Q9KIZ8
PmId
[10649995] Cloning and heterologous expression of the epothilone gene cluster. (Science. , 2000)
[10831849] Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum. (Gene. , 2000)
comment
3rd(Q9KIZ8) 99%, 0.0
Sorangium cellulosum (Polyangium cellulosum)_epoC
EpoC

Strain SMP44
Streptomyces coelicolorでepothilone biosynthetic gene clusterを発現してepothilone A and B産生を確認。

close this sectionPKS/NRPS Module

2 methylmalonyl-CoA
not conserved YASHS(Y->V)
KS8..384
AT538..854
DH903..1071
KR1441..1621
ACP1721..1790

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase]
MEEQESSAIAVIGMSGRFPGARDLDEFWRNLRDGTEAVQRFSEQELAASGVDPALVLDPS
YVRAGSVLEDVDRFDAAFFGISPREAELMDPQHRIFMECAWEALENAGYDPTAYEGSIGV
YAGANMSSYLTSNLHEHPAMMRWPGWFQTLIGNDKDYLATHVSYRLNLRGPSISVQTACS
TSLVAVHLACMSLLDRECDMALAGGITVRIPHRAGYVYAEGGIFSPDGHCRAFDAKANGT
IMGNGCGVVLLKPLDRALSDGDPVRAVILGSATNNDGARKIGFTAPSEVGQAQAIMEALA
LAGVEARSIQYIETHGTGTLLGDAIETAALRRVFGRDASARRSCAIGSVKTGIGHLESAA
GIAGLIKTVLALEHRQLPPSLNFESPNPSIDFASSPFYVNTSLKDWNTGSTPRRAGVSSF
GIGGTNAHVVLEEAPAAKLPAAAPARSAELFVVSAKSAAALDAAAARLRDHLQAHQGISL
GDVAFSLATTRSPMEHRLAMAAPSREALREGLDAAARGQTPPGAVRGRCSPGNVPKVVFV
FPGQGSQWVGMGRQLLAEEPVFHAALSACDRAIQAEAGWSLLAELAADEGSSQLERIDVV
QPVLFALAVAFAALWRSWGVAPDVVIGHSMGEVAAAHVAGALSLEDAVAIICRRSRLLRR
ISGQGEMAVTELSLAEAEAALRGYEDRVSVAVSNSPRSTVLSGEPAAIGEVLSSLNAKGV
FCRRVKVDVASHSPQVDPLREDLLAALGGLRPGAAAVPMRSTVTGAMVAGPELGANYWMN
NLRQPVRFAEVVQAQLQGGHGLFVEMSPHPILTTSVEEMRRAAQRAGAAVGSLRRGQDER
PAMLEALGTLWAQGYPVPWGRLFPAGGRRVPLPTYPWQRERYWIEAPAKSAAGDRRGVRA
GGHPLLGEMQTLSTQTSTRLWETTLDLKRLPWLGDHRVQGAVVFPGAAYLEMAISSGAEA
LGDGPLQITDVVLAEALAFAGDAAVLVQVVTTEQPSGRLQFQIASRAPGAGHASFRVHAR
GALLRVERTEVPAGLTLSAVRARLQASIPAAATYAELTEMGLQYGPAFQGIAELWRGEGE
ALGRVRLPDAAGSAAEYRLHPALLDACFQIVGSLFARSGEATPWVPVELGSLRLLQRPSG
ELWCHARVVNHGHQTPDRQGADFWVVDSSGAVVAEVCGLVAQRLPGGVRRREEDDWFLEL
EWEPAAVGTAKVNAGRWLLLGGGGGLGAALRAMLEAGGHAVVHAAENNTSAAGVRALLAK
AFDGQAPTAVVHLGSLDGGGELDPGLGAQGALDAPRSADVSPDALDPALVRGCDSVLWTV
QALAGMGFRDAPRLWLLTRGAQAVGAGDVSVTQAPLLGLGRVIAMEHADLRCARVDLDPA
RPEGELAALLAELLADDAEAEVALRGGERCVARIVRRQPETRPRGRIESCVPTDVTIRAD
STYLVTGGLGGLGLSVAGWLAERGAGHLVLVGRSGAASVEQRAAVAALEARGARVTVAKA
DVADRAQLERILREVTTSGMPLRGVVHAAGILDDGLLMQQTPARFRKVMAPKVQGALHLH
ALTREAPLSFFVLYASGVGLLGSPGQGNYAAANTFLDALAHHRRAQGLPALSVDWGLFAE
VGMAAAQEDRGARLVSRGMRSLTPDEGLSALARLLESGRAQVGVMPVNPRLWVELYPAAA
SSRMLSRLVTAHRASAGGPAGDGDLLRRLAAAEPSARSALLEPLLRAQISQVLRLPEGKI
EVDAPLTSLGMNSLMGLELRNRIEAMLGITVPATLLWTYPTVAALSGHLAREACEAAPVE
SPHTTADSAVEIEEMSQDDLTQLIAAKFKALT
selected fasta
>polyketide synthase [polyketide synthase]
ATGGAAGAACAAGAGTCCTCCGCTATCGCAGTCATCGGCATGTCGGGCCGTTTTCCGGGG
GCGCGGGATCTGGACGAATTCTGGAGGAACCTTCGAGACGGCACGGAGGCCGTGCAGCGC
TTCTCCGAGCAGGAGCTCGCGGCGTCCGGAGTCGACCCCGCGCTGGTGCTGGACCCGAGC
TACGTCCGGGCGGGCAGCGTGCTGGAAGACGTCGACCGGTTCGACGCTGCTTTCTTCGGC
ATCAGCCCGCGCGAGGCAGAGCTCATGGATCCGCAGCACCGGATCTTCATGGAATGCGCC
TGGGAGGCGCTGGAGAACGCCGGATACGACCCGACGGCTTACGAGGGCTCTATCGGCGTG
TACGCCGGCGCCAACATGAGCTCGTACTTGACGTCGAACCTCCACGAGCACCCAGCGATG
ATGCGGTGGCCCGGCTGGTTTCAGACGTTGATCGGCAACGACAAGGATTACCTCGCGACC
CACGTCTCCTACAGGCTGAATCTGAGAGGGCCGAGCATCTCCGTTCAAACTGCCTGCTCC
ACCTCGCTCGTGGCGGTTCACTTGGCGTGCATGAGCCTCCTGGACCGCGAGTGCGACATG
GCGCTGGCCGGCGGGATTACCGTCCGGATCCCCCATCGAGCCGGCTATGTATATGCTGAG
GGGGGCATCTTCTCTCCCGACGGCCATTGCCGGGCCTTCGACGCCAAGGCGAACGGCACG
ATCATGGGCAACGGCTGCGGCGTTGTCCTCCTGAAGCCGCTGGACCGGGCGCTCTCCGAT
GGTGATCCCGTCCGCGCGGTTATCCTTGGGTCTGCCACAAACAACGACGGAGCGAGGAAG
ATCGGGTTCACTGCGCCCAGTGAGGTGGGCCAGGCGCAAGCGATCATGGAGGCGCTGGCG
CTGGCAGGGGTCGAGGCCCGGTCCATCCAATACATCGAGACCCACGGGACCGGCACGCTG
CTCGGAGACGCCATCGAGACGGCGGCGCTGCGGCGGGTGTTCGGTCGCGACGCTTCGGCC
CGGAGGTCTTGCGCGATCGGCTCCGTGAAGACCGGCATCGGACACCTCGAATCGGCGGCT
GGCATCGCCGGTTTGATCAAGACGGTCTTGGCGCTGGAGCACCGGCAGCTGCCGCCCAGC
CTGAACTTCGAGTCTCCTAACCCATCGATCGATTTCGCGAGCAGCCCGTTCTACGTCAAT
ACCTCTCTTAAGGATTGGAATACCGGCTCGACTCCGCGGCGGGCCGGCGTCAGCTCGTTC
GGGATCGGCGGCACCAACGCCCATGTCGTGCTGGAGGAAGCGCCCGCGGCGAAGCTTCCA
GCCGCGGCGCCGGCGCGCTCTGCCGAGCTCTTCGTCGTCTCGGCCAAGAGCGCAGCGGCG
CTGGATGCCGCGGCGGCACGGCTACGAGATCATCTGCAGGCGCACCAGGGGATTTCGTTG
GGCGACGTCGCCTTCAGCCTGGCGACGACGCGCAGCCCCATGGAGCACCGGCTCGCGATG
GCGGCGCCGTCGCGCGAGGCGTTGCGAGAGGGGCTCGACGCAGCGGCGCGAGGCCAGACC
CCGCCGGGCGCCGTGCGTGGCCGCTGCTCCCCAGGCAACGTGCCGAAGGTGGTCTTCGTC
TTTCCCGGCCAGGGCTCTCAGTGGGTCGGCATGGGCCGGCAGCTCCTGGCTGAGGAACCC
GTCTTCCACGCGGCGCTTTCGGCGTGCGACCGGGCCATCCAGGCCGAAGCTGGTTGGTCG
CTGCTCGCGGAGCTCGCCGCCGACGAAGGGTCCTCCCAGCTCGAGCGCATCGACGTGGTG
CAGCCGGTGCTGTTCGCCCTCGCGGTGGCATTTGCGGCGCTGTGGCGGTCGTGGGGTGTC
GCGCCCGACGTCGTGATCGGCCACAGCATGGGCGAGGTAGCCGCCGCGCATGTGGCCGGG
GCGCTGTCGCTCGAGGATGCGGTGGCGATCATCTGCCGGCGCAGCCGGCTGCTCCGGCGC
ATCAGCGGTCAGGGCGAGATGGCGGTGACCGAGCTGTCGCTGGCCGAGGCCGAGGCGGCG
CTCCGAGGCTACGAGGATCGGGTGAGCGTGGCCGTGAGCAACAGCCCGCGCTCGACGGTG
CTCTCGGGCGAGCCGGCAGCGATCGGCGAGGTGCTGTCGTCCCTGAACGCGAAGGGGGTG
TTCTGCCGTCGGGTGAAGGTGGATGTCGCCAGCCACAGCCCGCAGGTCGACCCGCTGCGC
GAGGACCTCTTGGCAGCCCTGGGCGGGCTCCGGCCGGGTGCGGCTGCGGTGCCGATGCGC
TCGACGGTGACGGGCGCCATGGTAGCGGGCCCGGAGCTCGGAGCGAATTACTGGATGAAC
AACCTCAGGCAGCCAGTGCGCTTCGCCGAGGTAGTCCAGGCGCAGCTCCAAGGCGGCCAC
GGTCTGTTCGTGGAGATGAGCCCGCATCCGATCCTAACGACTTCGGTCGAGGAGATGCGG
CGCGCGGCCCAGCGGGCGGGCGCAGCGGTGGGCTCGCTGCGGCGGGGGCAGGACGAGCGC
CCGGCGATGCTGGAGGCGCTGGGCACGCTGTGGGCGCAGGGCTACCCTGTACCCTGGGGG
CGGCTGTTTCCCGCGGGGGGGCGGCGGGTACCGCTGCCGACCTATCCCTGGCAGCGCGAG
CGGTACTGGATCGAAGCGCCGGCCAAGAGCGCCGCGGGCGATCGCCGCGGCGTGCGTGCG
GGCGGTCACCCGCTCCTCGGTGAAATGCAGACCCTGTCAACCCAGACGAGCACGCGGCTG
TGGGAGACGACGCTGGATCTCAAGCGGCTGCCGTGGCTCGGCGACCACCGGGTGCAGGGA
GCGGTCGTGTTTCCGGGCGCGGCGTACCTGGAGATGGCGATTTCGTCGGGGGCCGAGGCT
TTGGGCGATGGCCCTTTGCAGATAACTGACGTGGTGCTCGCCGAGGCGCTGGCCTTCGCG
GGCGACGCGGCGGTGTTGGTCCAGGTGGTGACGACGGAGCAGCCGTCGGGGCGGCTGCAG
TTCCAGATCGCGAGCCGGGCGCCGGGCGCTGGCCACGCGTCCTTCCGGGTCCACGCTCGC
GGCGCGTTGCTCCGAGTGGAGCGCACCGAGGTCCCGGCTGGGCTTACGCTTTCCGCTGTG
CGCGCGCGGCTCCAGGCCAGCATACCCGCCGCGGCCACCTACGCGGAGCTGACCGAGATG
GGGCTGCAGTACGGCCCTGCCTTCCAGGGGATTGCTGAGCTATGGCGGGGTGAAGGCGAG
GCGCTGGGACGGGTACGCCTGCCCGACGCGGCCGGCTCGGCAGCGGAGTATCGGTTGCAT
CCTGCGCTGCTGGACGCGTGCTTCCAGATCGTCGGCAGCCTCTTCGCCCGCAGTGGCGAG
GCGACGCCGTGGGTGCCCGTGGAGTTGGGCTCGCTGCGGCTCTTGCAGCGGCCTTCGGGG
GAGCTGTGGTGCCATGCGCGCGTCGTGAACCATGGGCACCAAACCCCCGATCGGCAGGGC
GCCGACTTTTGGGTGGTCGACAGCTCGGGTGCAGTGGTCGCCGAAGTTTGCGGGCTCGTG
GCGCAGCGGCTTCCGGGAGGGGTGCGCCGGCGCGAAGAAGACGATTGGTTCCTGGAGCTC
GAGTGGGAACCCGCAGCGGTCGGCACAGCCAAGGTCAACGCGGGCCGGTGGCTGCTCCTC
GGCGGCGGCGGTGGGCTCGGCGCCGCGTTGCGCGCGATGCTGGAGGCCGGCGGCCATGCC
GTCGTGCATGCGGCAGAGAACAACACGAGCGCTGCCGGCGTACGCGCGCTCCTGGCAAAG
GCCTTTGACGGCCAGGCTCCGACGGCGGTGGTGCACCTCGGCAGCCTCGATGGGGGTGGC
GAGCTCGACCCAGGGCTCGGGGCGCAAGGCGCATTGGACGCGCCCCGGAGCGCCGACGTC
AGTCCCGATGCCCTCGATCCGGCGCTGGTACGTGGCTGCGACAGCGTGCTCTGGACCGTG
CAGGCCCTGGCCGGCATGGGCTTTCGAGACGCCCCGCGATTGTGGCTTTTGACCCGCGGC
GCACAGGCCGTCGGCGCCGGCGACGTCTCCGTGACACAGGCACCGCTGCTGGGGCTGGGC
CGCGTCATCGCCATGGAGCACGCGGATCTGCGCTGCGCTCGGGTCGACCTCGATCCAGCC
CGGCCCGAGGGGGAGCTCGCTGCCCTGCTGGCCGAGCTGCTGGCCGACGACGCCGAAGCG
GAAGTCGCGTTGCGCGGTGGCGAGCGATGCGTCGCTCGGATCGTCCGCCGGCAGCCCGAG
ACCCGGCCCCGGGGGAGGATCGAGAGCTGCGTTCCGACCGACGTCACCATCCGCGCGGAC
AGCACCTACCTTGTGACCGGCGGTCTGGGTGGGCTCGGTCTGAGCGTGGCCGGATGGCTG
GCCGAGCGCGGCGCTGGTCACCTGGTGCTGGTGGGCCGCTCCGGCGCGGCGAGCGTGGAG
CAACGGGCAGCCGTCGCGGCGCTCGAGGCCCGCGGCGCGCGCGTCACCGTGGCGAAGGCG
GATGTCGCCGATCGGGCGCAGCTCGAGCGGATCCTCCGCGAGGTTACCACGTCGGGGATG
CCGCTGCGGGGCGTCGTCCATGCGGCCGGCATCTTGGACGACGGGCTGCTGATGCAGCAG
ACTCCCGCGCGGTTTCGTAAGGTGATGGCGCCCAAGGTCCAGGGGGCCTTGCACCTGCAC
GCGTTGACGCGCGAAGCGCCGCTTTCCTTCTTCGTGCTGTACGCTTCGGGAGTAGGGCTC
TTGGGCTCGCCGGGCCAGGGCAACTACGCCGCGGCCAACACGTTCCTCGACGCTCTGGCG
CACCACCGGAGGGCGCAGGGGCTGCCAGCGTTGAGCGTCGACTGGGGCCTGTTCGCGGAG
GTGGGCATGGCGGCCGCGCAGGAAGATCGCGGCGCGCGGCTGGTCTCCCGCGGAATGCGG
AGCCTCACCCCCGACGAGGGGCTGTCCGCTCTGGCACGGCTGCTCGAAAGCGGCCGCGCT
CAGGTGGGGGTGATGCCGGTGAACCCGCGGCTGTGGGTGGAGCTCTACCCCGCGGCGGCG
TCTTCGCGAATGTTGTCGCGCCTGGTGACGGCGCATCGCGCGAGCGCCGGCGGGCCAGCC
GGGGACGGGGACCTGCTCCGCCGCCTCGCCGCTGCCGAGCCGAGCGCGCGGAGCGCGCTC
CTGGAGCCGCTCCTCCGCGCGCAGATCTCGCAGGTGCTGCGCCTCCCCGAGGGCAAGATC
GAGGTGGACGCCCCGCTCACGAGCCTGGGCATGAACTCGCTGATGGGGCTCGAGCTGCGC
AACCGCATCGAGGCCATGCTGGGCATCACCGTACCGGCAACGCTGTTGTGGACCTATCCC
ACGGTGGCGGCGCTGAGCGGGCATCTGGCGCGGGAGGCATGCGAAGCCGCTCCTGTGGAG
TCACCGCACACCACCGCCGACTCTGCCGTCGAGATCGAGGAGATGTCGCAGGACGATCTG
ACGCAGTTGATCGCAGCAAAATTCAAGGCGCTTACATGA
[2] KS8..384
[2] AT538..854
[2] methylmalonyl-CoA not conserved YASHS(Y->V)729..733
[2] DH903..1071
[2] KR1441..1621
[2] ACP1721..1790
[2] KS22..1152
[2] AT1612..2562
[2] methylmalonyl-CoA not conserved YASHS(Y->V)2185..2199
[2] DH2707..3213
[2] KR4321..4863
[2] ACP5161..5370

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[533-661] 5.69999999999998e-76 G3DSA:3.40.366.10 [729-846] 5.69999999999998e-76 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1748-1763] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1725-1789] 4.9e-13 PF00550
PF00550   PP-binding
[1721-1790] PS50075
PS50075   ACP_DOMAIN
[1714-1792] 4.70000326671387e-20 SSF47336
SSF47336   ACP_like
[1721-1792] 4.70000000000001e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[1441-1620] 2.30000000000004e-72 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[8-257] 1.69999999999998e-76 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[266-384] 1.09999999999999e-41 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[538-854] 7.90000000000016e-98 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[536-838] 2.89997807423186e-68 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[663-728] 2.19999900980708e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[8-268] 3.30000000000002e-79 G3DSA:3.40.47.10 [270-436] 3.80000000000003e-63 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[6-436] 5.6999970878395e-85 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1440-1657] 2.1e-46 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[170-186] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[540-837] 6.4999795054176e-115 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1722-1793] 1.10000150671642e-24 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[903-1071] 1.40000506907309e-52 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[9-436] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1441-1621] 3.1999989904635e-63 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Epoth_00070 Epoth_00070   Epoth_00090 Epoth_00090
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