FR008_00030 : CDS information

Location

Organism	Streptomyces sp.
Strain	FR-008
Entry name	FR-008
Contig	AY310323
Start / Stop / Direction	3,818 / 3,150 / - [in whole cluster] 3,818 / 3,150 / - [in contig]
Location	complement(3150..3818) [in whole cluster] complement(3150..3818) [in contig]
Type	CDS
Length	669 bp (222 aa)

Annotation

Category	4.1 transcriptional regulator
Product	LuxR family transcriptional regulator
Product (GenBank)	FscRI
Gene
Gene (GenBank)	fscRI
EC number
Keyword
Note
Note (GenBank)	putative transcriptional activator
Reference	ACC Q6W5R3 PmId [14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003) [25575546] Production of the antibiotic FR-008/candicidin in Streptomyces sp. FR-008 is co-regulated by two regulators, FscRI and FscRIV, from different transcription factor families. (Microbiology. , 2015) comment [PMID: 14652074](2003) complete FR-008 gene clusterの報告。 fscRI: transcriptional regulator @regulation 4つのputative regulatory genes(fscRI, fscRII, fscRIII, and fscRIV)の推定産物はすべてtranscriptional regulatorsのLuxR familyに属しており、FR-008生合成の調節に関与しているかもしれない。そのようなtranscriptional regulatorsは大きなPKS genesの非常に長いmRNAsの安定性を維持するために働くかもしれない。 fscRII、fscRIIIと部分的なfscRIを含む部分を置換したmutantでは、いかなるFR-008-related compoundsも産生しなかったことから、putative regulatory activitiesが裏付けられた。 --- [PMID: 25575546](2015) fscRI と fscRIV のdeletion mutantを作成し、FR-008産生や関連する遺伝子の発現について調査。fscRI と fscRIV は相互制御性であることが示唆された。
Related Reference	ACC Q9L4V8 NITE Nysta_00200 PmId [14973031] In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis. (J Bacteriol. , 2004) comment BLAST id66% Streptomyces noursei_nysRIV [Nysta_00200]transcriptional regulator regulatory genes候補の不活化mutantにおいて、nystatin gene cluster内にあるputative promoter regionsの調節下に置いたxylE reporter gene活性を測定。不活化mutantのCross-complementation experiments。 nysRIVはnysRI, RII, RIII のmutantsを相補できるのだが、逆にそれらのregulatorsはnysRIV mutantを相補できないので、nysRIVは最も直接的にnystatin生合成遺伝子の発現を調節しているのだろう。

Sequence

>LuxR family transcriptional regulator [FscRI]
MDPAPAADPAGSPERAAVLADGFDRAGAYVACLDPSLTIQQVNQEFERRFGGSSSELCGS
RFCDLVHPSVQQPLMHQFARMLDGKRHRFATEVIAVDQERTASTLPLNALAVRGGRTPDV
AAILVVMNAAEEEAGDADVMAPRKKLLSEIDARILEGIAAGVSTIPLASRLYLSRQGVEY
HVTGLLRKLKVPNRAALVSRAYSMGVLKVGTWPPKVVEDFIK

>LuxR family transcriptional regulator [FscRI]
ATGGATCCCGCGCCGGCGGCCGACCCGGCCGGCTCGCCGGAACGGGCCGCCGTTCTCGCG
GACGGGTTCGACCGGGCCGGTGCCTATGTCGCCTGCCTCGACCCGTCTCTCACGATCCAG
CAGGTCAACCAGGAGTTCGAGCGCCGCTTCGGCGGCTCGTCCTCCGAACTCTGCGGCAGC
CGCTTCTGCGACCTCGTCCACCCGAGCGTCCAGCAGCCCCTCATGCACCAGTTCGCGCGG
ATGCTGGACGGCAAGCGGCACCGCTTCGCCACCGAGGTCATCGCGGTCGACCAGGAACGC
ACGGCGTCCACGCTGCCGCTGAACGCGCTCGCGGTACGCGGTGGCCGTACGCCCGACGTG
GCCGCGATCCTCGTGGTGATGAACGCCGCGGAGGAGGAGGCCGGGGACGCGGACGTCATG
GCGCCCCGCAAGAAGCTCCTCAGCGAGATCGACGCACGCATCCTGGAGGGCATAGCCGCC
GGGGTCTCGACCATCCCGCTCGCCTCCCGCCTCTACCTCAGCCGCCAGGGCGTGGAGTAC
CACGTCACCGGGCTGCTGCGGAAGCTCAAGGTGCCCAACCGTGCCGCTCTCGTCTCCCGC
GCCTACTCCATGGGCGTCCTCAAGGTCGGCACCTGGCCGCCGAAGGTCGTCGAGGACTTC
ATCAAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000014 PAS domain (Domain) TIGR00229 sensory_box: PAS domain S-box protein SM00091 PAS domain IPR000792 Transcription regulator LuxR, C-terminal (Domain) PF00196 GerE SM00421 helix_turn_helix, Lux Regulon IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 no description IPR013656 PAS fold-4 (Domain) PF08448 PAS_4 IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 C-terminal effector domain of the bipartite response regulators
SignalP	Eukaryota
TMHMM	No significant hit

FR008_00020 FR008_00040