FR008_00050 : CDS information

Location

Organism	Streptomyces sp.
Strain	FR-008
Entry name	FR-008
Contig	AY310323
Start / Stop / Direction	10,254 / 7,210 / - [in whole cluster] 10,254 / 7,210 / - [in contig]
Location	complement(7210..10254) [in whole cluster] complement(7210..10254) [in contig]
Type	CDS
Length	3,045 bp (1,014 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative LuxR family transcriptional regulator
Product (GenBank)	FscRIII
Gene
Gene (GenBank)	fscRIII
EC number
Keyword
Note
Note (GenBank)	putative transcriptional regulator
Reference	ACC Q6W5R1 PmId [14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003) comment complete FR-008 gene clusterの報告。 fscRIII: transcriptional regulator @regulation 4つのputative regulatory genes(fscRI, fscRII, fscRIII, and fscRIV)の推定産物はすべてtranscriptional regulatorsのLuxR familyに属しており、FR-008生合成の調節に関与しているかもしれない。そのようなtranscriptional regulatorsは大きなPKS genesの非常に長いmRNAsの安定性を維持するために働くかもしれない。 fscRII、fscRIIIと部分的なfscRIを含む部分を置換したmutantでは、いかなるFR-008-related compoundsも産生しなかったことから、putative regulatory activitiesが裏付けられた。
Related Reference	ACC Q9L4W0 NITE Nysta_00180 PmId [14973031] In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis. (J Bacteriol. , 2004) comment 2nd(Q9L4W0) 36%, 1e-101 Streptomyces noursei_nysRII [Nysta_00180]transcriptional regulator regulatory genes候補の不活化mutantにおいて、nystatin gene cluster内にあるputative promoter regionsの調節下に置いたxylE reporter gene活性を測定。不活化mutantのCross-complementation experiments。 nysRII mutantは、nysRII自身で完全に、nysRIVで77%まで相補できる。 NysRIIはnysRI promoterからの効率的転写に必要。 nysRI, II, IIIがないとnysRIV promoterには作用がない。 NysRI, II, III IVは効率的なnystatin産生のために不可欠である。

Sequence

>putative LuxR family transcriptional regulator [FscRIII]
MYDLHMPPIELPLLERDRELAALSAVIGELGSGRPAVVTVTGEPGLGQNDLLRWAAAYAT
DAGLRVLSAHATPAEHEVRYGVVAQLLAEENRALAPRLFLTDEQPGGLPGLNGLLNAVRD
RPTLLVVRDTQWLDPASLRWLEALARRLPRAPVALLTSTTGTALTRPEWSVGTPLTGLAT
TIELALPPLTSGGTATAVLRAFGAPGDPAFTEALAEATRGIPAVVHDVLDRFARAGYSPR
ADRIDPLRALTAEVVGDHATRALSDLRDPAAVDALRALAVCGDLLDFPLVCTLAGPHSVS
ESRLRAALAASGLTTLRDGHPRVQDAVVRARVLEEMPAADRAELYARAAGLAQRVAADDQ
GIADLLLLARPVGDPWAVDTLRRGFTSALRGGRRDLAVAYLARALDEPLAAEDRARIEFQ
LASVEMVTAPTAAERRLGGLIRATRPGPGAGLRARATDLCLLGGDTRAARHALAGAIDSA
PAAPEPRRGPTGAPAVPPEAEPASTPRTHLVPHRPYAGTPPGATGPDGRRSAAPDLPGTP
RAHDAPRSPGPEAAGRPDTAGPDEDPAVAERHELTVLCRVASFLRHDDADLDVLPVPALP
DAVHNPAVAGVWAWEHGVAGVHREEVRRLARSAFVPAATGRPLLVVPRLLGCRALLLTDD
GDEAENHLGALLDESHRERAAISVAHILTVRAELHIRHGRPDAAARDLAAAQAELPLDRL
HPLFLPYWLALSMITDLQNGHTERARETAARPLPPLAHESATTAQLLFARGVLARTDDEP
HQAREHFRACGRWLLRHGCANPAVQPWRSLAAEAAHTLGDTEEAARLVHEEVRLARRWGA
AAPLGRAQLSLAVVTEENRVENLRAAVTTLSAASARTAYTRAVIELAAAEREENERRTAV
ALGPAATVLPPVAPPPGPHVPSAVARPPGPRPAVLTGRRTAKAPGHDAAVWHGLTAVERE
TAALAAQGLGNREIATELAVTTRAVELRLSGVYRKLRIRGREELRALVQEAEGS

>putative LuxR family transcriptional regulator [FscRIII]
ATGTATGATCTTCACATGCCGCCCATCGAGCTCCCGCTCCTGGAGCGCGACCGCGAACTG
GCCGCCCTCTCCGCCGTCATCGGCGAGCTCGGATCCGGCCGTCCCGCGGTGGTGACGGTG
ACCGGAGAACCGGGGCTCGGCCAGAACGATCTGCTGCGCTGGGCGGCCGCGTACGCCACC
GACGCCGGACTGCGCGTCCTCAGCGCCCACGCCACCCCCGCCGAACACGAGGTGCGGTAC
GGCGTGGTGGCCCAGCTCCTCGCGGAGGAGAACCGCGCACTCGCCCCGCGCCTCTTCCTC
ACCGACGAGCAGCCCGGCGGGCTGCCGGGCCTCAACGGCCTGCTCAACGCCGTCCGCGAC
CGGCCCACCCTGCTCGTGGTACGCGACACCCAGTGGCTCGACCCGGCCTCCCTGCGCTGG
CTGGAAGCCCTCGCCCGGCGGCTGCCCCGCGCCCCGGTCGCCCTGCTCACCAGCACCACC
GGCACCGCCCTGACCCGGCCCGAGTGGAGCGTCGGCACCCCCCTGACCGGACTGGCCACC
ACCATCGAGCTGGCGCTGCCGCCCCTCACCTCCGGCGGCACCGCCACCGCCGTCCTGCGG
GCCTTCGGCGCCCCGGGGGACCCCGCCTTCACCGAGGCCCTCGCCGAGGCGACCCGGGGC
ATCCCGGCCGTCGTCCACGACGTGCTGGACCGTTTCGCCCGCGCGGGGTACAGCCCCCGG
GCCGACCGGATCGACCCCCTGCGCGCCCTGACCGCGGAGGTCGTCGGCGACCACGCCACC
CGCGCCCTCAGCGACCTGCGCGACCCGGCCGCCGTCGACGCCCTGCGCGCCCTCGCCGTC
TGCGGCGACCTCCTCGACTTCCCCCTCGTCTGCACGCTCGCCGGACCGCACTCCGTCTCC
GAGTCCCGCCTGCGCGCCGCCCTGGCGGCCAGCGGCCTGACCACCCTGCGCGACGGCCAC
CCCCGGGTCCAGGACGCCGTCGTACGCGCCCGCGTCCTGGAGGAGATGCCCGCAGCGGAC
CGGGCCGAGCTGTACGCACGCGCCGCCGGGCTCGCCCAGCGCGTCGCCGCCGACGACCAG
GGCATAGCCGACCTGCTGCTGCTGGCCCGCCCCGTCGGCGACCCCTGGGCCGTCGACACC
CTGCGGCGGGGCTTCACCTCCGCGCTGCGCGGCGGCCGCCGCGACCTGGCCGTCGCCTAC
CTGGCGCGCGCCCTGGACGAACCACTGGCCGCCGAGGACCGCGCCCGGATCGAGTTCCAG
CTGGCCTCCGTGGAGATGGTCACCGCCCCCACGGCCGCCGAACGCCGGCTGGGCGGGCTC
ATCCGGGCCACCCGGCCCGGCCCCGGCGCCGGACTGCGGGCCCGCGCCACCGACCTGTGC
CTCCTCGGCGGAGACACCCGCGCGGCCCGCCACGCCCTCGCCGGAGCCATCGACTCGGCC
CCGGCCGCCCCCGAACCACGACGTGGCCCGACCGGCGCCCCGGCCGTGCCGCCCGAGGCG
GAGCCCGCCTCGACTCCCCGCACCCACCTCGTCCCTCACCGCCCCTACGCGGGCACCCCG
CCCGGCGCCACCGGGCCGGACGGTAGAAGATCCGCCGCGCCGGACCTTCCCGGCACCCCC
CGCGCCCACGACGCCCCCCGCTCTCCCGGCCCCGAGGCCGCAGGACGACCGGACACCGCC
GGGCCGGACGAGGACCCGGCCGTCGCCGAACGGCACGAACTGACCGTCCTGTGCCGCGTC
GCCTCCTTCCTCCGCCACGACGACGCCGACCTCGACGTCCTCCCCGTCCCCGCCCTGCCC
GACGCGGTGCACAACCCGGCCGTCGCGGGGGTCTGGGCCTGGGAACACGGCGTGGCCGGC
GTCCACCGCGAGGAAGTACGCAGACTCGCCCGCTCCGCCTTCGTCCCCGCCGCCACCGGC
CGCCCGCTCCTCGTCGTACCCCGGCTGCTCGGCTGCCGCGCCCTCCTGCTCACCGACGAC
GGCGACGAGGCGGAGAACCACCTCGGTGCCCTGCTCGACGAGTCCCACCGGGAGCGCGCG
GCGATCTCGGTCGCCCACATCCTCACCGTCCGCGCCGAGCTGCACATCCGCCACGGCCGC
CCCGACGCCGCCGCCCGTGACCTGGCCGCCGCCCAGGCGGAACTCCCGCTGGACCGCCTC
CACCCGCTCTTCCTCCCGTACTGGCTCGCCCTGAGCATGATCACCGATCTGCAGAACGGC
CACACCGAACGGGCCCGCGAGACCGCCGCCCGGCCGCTGCCGCCGCTCGCCCACGAGAGC
GCCACCACCGCCCAACTGCTCTTCGCCCGGGGGGTGCTGGCCCGCACGGACGACGAGCCC
CACCAGGCCAGGGAACACTTCCGCGCCTGCGGTCGCTGGCTCCTGCGGCACGGCTGCGCC
AACCCGGCCGTCCAGCCCTGGCGCTCCCTCGCCGCCGAGGCCGCCCACACCCTCGGCGAC
ACCGAGGAGGCCGCCCGGCTGGTCCACGAGGAAGTCCGCCTCGCCCGCCGCTGGGGCGCC
GCCGCCCCGCTCGGCCGCGCCCAGCTCAGCCTCGCCGTCGTCACCGAGGAGAACCGGGTC
GAAAACCTCCGTGCCGCCGTCACCACGCTCAGCGCGGCATCGGCCCGCACCGCCTACACC
CGGGCCGTCATCGAACTGGCCGCCGCCGAACGCGAGGAGAACGAGCGCCGGACCGCCGTC
GCCCTCGGCCCGGCCGCCACGGTGCTCCCGCCGGTCGCCCCACCCCCCGGTCCGCACGTC
CCCTCCGCGGTGGCCAGGCCCCCCGGCCCGCGCCCGGCGGTGCTGACCGGTCGGCGGACC
GCGAAGGCCCCGGGCCACGACGCGGCCGTCTGGCACGGCCTGACCGCCGTCGAACGGGAG
ACCGCGGCCCTCGCCGCCCAGGGCCTGGGCAACCGGGAGATCGCCACGGAACTCGCCGTG
ACCACGCGCGCCGTGGAGCTGCGCCTCAGCGGCGTCTACCGCAAACTCCGCATCCGCGGA
CGTGAGGAACTGCGCGCACTCGTACAAGAAGCGGAGGGCAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) SM00421 HTH_LUXR PF00196 GerE IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

FR008_00040 FR008_00060