FR008_00100 FR008_00100   FR008_00120 FR008_00120

FR008_00110 : CDS information

close this sectionLocation

Organism
StrainFR-008
Entry nameFR-008
Contig
Start / Stop / Direction17,556 / 18,413 / + [in whole cluster]
17,556 / 18,413 / + [in contig]
Location17556..18413 [in whole cluster]
17556..18413 [in contig]
TypeCDS
Length858 bp (285 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Producttype II thioesterase
Product (GenBank)FscTE
Gene
Gene (GenBank)fscTE
EC number
Keyword
  • type II thioesterase
Note
Note (GenBank)
  • type II thioesterase
Reference
ACC
PmId
[14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003)
[18836004] Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008. (Appl Environ Microbiol. , 2008)
comment
[PMID: 14652074](2003)
complete FR-008 gene clusterの報告。
fscTE: type II thioesterase
@removal of aberrant intermediates

---
[PMID: 18836004](2008)
fscTEはputative type II thioesterase (TEII)をコードする。
fscTE deletionでFR-008/candicidin産生は約90%減。
type I thioesteraseを不活化させたとき、FscTEは成熟伸長したpolyketideの放出を代償することができなかった。

FscTE and TylOのHydrolytic activitiesは、
propionyl-S-N-acetylcysteamine > methylmalonyl-S-N-acetylcysteamine
acetyl-S-N-acetylcysteamine > malonyl-S-N-acetylcysteamine

したがって、TEII はacyl carrier proteins(ACP)に結合する伸長できない残基を選択的に除去することにより、効果的なpolyketide生合成を維持することが結論付けられる。

close this sectionSequence

selected fasta
>type II thioesterase [FscTE]
MPRPARPSHPRTRRRRNTDRRQNHRRDSPVTTADDTAARWLRRYHPAEADAVRLVCFPHA
GGSASFYHPVSARFAPGAEVVSLQYPGRQDRRKEPCVPDLGTLADLITEQLLPLDERPTV
FFGHSMGAALAFETAWRLEQKGAGPRTVIASGRRGPSTTRAERVHTRDDDGIVAEMKRLN
GTAAGVLGDEEILRMALPALRGDYRAIETYTCPPDRRLACGLTVLTGEDDPLTTVEEAER
WRDHTTGPFRLRVFTGGHFFLTQHLDAVNTEIAQALHPDRAAPAA
selected fasta
>type II thioesterase [FscTE]
GTGCCCCGTCCGGCCCGGCCCTCGCACCCGCGGACCCGTCGCCGGCGGAACACCGACCGG
CGCCAGAACCATCGAAGGGACTCCCCCGTGACCACCGCTGACGACACCGCGGCCCGCTGG
CTGCGGCGGTACCACCCGGCCGAGGCCGACGCGGTACGGCTGGTGTGCTTCCCGCACGCC
GGCGGCTCCGCCAGCTTCTACCACCCGGTCTCGGCGCGGTTCGCGCCGGGCGCCGAGGTC
GTCTCGCTCCAGTACCCGGGCCGGCAGGACCGCCGCAAGGAGCCCTGTGTCCCGGACCTC
GGCACGCTGGCCGACCTGATCACCGAGCAGCTGCTCCCGCTGGACGAGCGGCCCACCGTC
TTCTTCGGGCACAGCATGGGGGCCGCGCTCGCCTTCGAGACGGCGTGGCGGCTGGAGCAG
AAGGGCGCCGGTCCCCGCACCGTCATCGCCTCCGGACGGCGCGGCCCCTCCACCACCCGC
GCCGAACGGGTCCACACGAGGGACGACGACGGGATCGTCGCGGAGATGAAGCGGCTGAAC
GGCACGGCGGCCGGTGTCCTCGGCGACGAGGAGATCCTCCGCATGGCGCTGCCCGCGCTG
CGCGGCGACTACCGCGCCATCGAGACCTACACCTGCCCTCCGGACCGCCGGCTGGCCTGC
GGGCTGACCGTGCTGACGGGCGAGGACGACCCGCTGACCACCGTCGAGGAGGCGGAGCGG
TGGCGCGACCACACCACCGGGCCGTTCCGGCTGCGGGTCTTCACGGGCGGGCACTTCTTC
CTCACCCAGCACCTCGACGCGGTCAACACGGAGATCGCCCAGGCCCTCCACCCCGACCGG
GCCGCCCCGGCCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[53-276] 1.40000000000001e-62 PF00975
PF00975   Thioesterase
SignalP No significant hit
TMHMM No significant hit
FR008_00100 FR008_00100   FR008_00120 FR008_00120
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