FR008_00090 FR008_00090   FR008_00110 FR008_00110

FR008_00100 : CDS information

close this sectionLocation

Organism
StrainFR-008
Entry nameFR-008
Contig
Start / Stop / Direction17,334 / 17,528 / + [in whole cluster]
17,334 / 17,528 / + [in contig]
Location17334..17528 [in whole cluster]
17334..17528 [in contig]
TypeCDS
Length195 bp (64 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)FscFE
Gene
Gene (GenBank)fscFE
EC number
Keyword
Note
Note (GenBank)
  • putative ferredoxin
Reference
ACC
PmId
[14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003)
comment
complete FR-008 gene clusterの報告。
fscFE: ferredoxin
@electron transfer in P450 system

FscP and FscFEはP450 monooxygenase systemを表す。
おそらくC18-methyl分枝→carboxyl基への酸化を担う。
Related Reference
ACC
P18324
PmId
[1846297] Ferredoxins from two sulfonylurea herbicide monooxygenase systems in Streptomyces griseolus. (Biochemistry. , 1991)
comment
16th(P18324) 57%, 6e-11
Streptomyces griseolus_suaB
Ferredoxin-1
metal-ion binding site保存あり

sulfonylurea herbicideが誘発されたStreptomyces griseolusの可溶性タンパク質フラクションより、Fd-1 と Fd-2の二種類のフェレドキシンを精製し、どちらも、aerobic mixture of NADPH、spinach ferredoxin:NADP oxidoreductase、精製したcytochrome P-450SU1とherbicide基質にsulfonylurea monooxygenase活性を復元できることが示されている。また、cytochromes P-450, P-450SU1 (CYP105A1) と P-450SU2 (CYP105B1)は、それぞれFd-1 と Fd-2の近傍に位置していることが示されている。

close this sectionSequence

selected fasta
>putative ferredoxin [FscFE]
MRVTVDSEQCVGAGQCVLNAPEVFDQDDDGVVVLLRAEPDERDHEAVRTAGDLCPSASVV
LQED
selected fasta
>putative ferredoxin [FscFE]
ATGCGTGTCACCGTCGACAGCGAGCAGTGCGTAGGAGCGGGCCAGTGCGTCCTGAACGCG
CCGGAGGTCTTCGACCAGGACGACGACGGCGTCGTGGTCCTGCTCCGCGCGGAGCCCGAC
GAGCGGGACCACGAGGCGGTCCGCACGGCGGGCGACCTGTGCCCGTCGGCCTCGGTCGTC
CTCCAGGAGGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001080 3Fe-4S ferredoxin (Family)
[5-16] 8.8e-06 PR00352 [16-26] 8.8e-06 PR00352 [47-59] 8.8e-06 PR00352
PR00352   3FE4SFRDOXIN
IPR010693 Ferredoxin soy-like (Family)
[1-60] 1.1e-13 PF06902
PF06902   DUF1271
IPR017896 4Fe-4S ferredoxin-type, iron-sulpur binding domain (Domain)
[1-29] 7.634 PS51379
PS51379   4FE4S_FER_2
SignalP No significant hit
TMHMM No significant hit
FR008_00090 FR008_00090   FR008_00110 FR008_00110
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