FR008_00080 FR008_00080   FR008_00100 FR008_00100

FR008_00090 : CDS information

close this sectionLocation

Organism
StrainFR-008
Entry nameFR-008
Contig
Start / Stop / Direction16,106 / 17,287 / + [in whole cluster]
16,106 / 17,287 / + [in contig]
Location16106..17287 [in whole cluster]
16106..17287 [in contig]
TypeCDS
Length1,182 bp (393 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)FscP
Gene
Gene (GenBank)fscP
EC number
Keyword
  • C-18 carboxy group formation
Note
Note (GenBank)
  • putative cytochrome P450 monooxygenase
Reference
ACC
PmId
[14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003)
[19139241] Tailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis. (Appl Environ Microbiol. , 2009)
comment
[PMID: 14652074](2003)
complete FR-008 gene clusterの報告。
fscP: cytochrome P450 monooxygenase
@formation of carboxyl group at C-18

---
[PMID: 19139241](2009)
fscP deletion mutantがcarboxyl基を持たないFR-008 derivativesを産生することを確認。その産物は毒性が低い。
また、fscP deletion mutantはfscP-fscFEを持ったplasmidで相補され、普通のFR-008/candicidin産生をwildレベルまで回復する。

close this sectionSequence

selected fasta
>cytochrome P450 [FscP]
MTTSPGPTVVDFPRRTPREPLPLSQYAEHRKQNGLVQTHLPNGRPIWLVTRHEDVRAVLT
HPRISANPDNEGFPNVGETMGVPKQEQIPGWFVGLDSPEHDRFRKVLIPEFTVRRVRELR
PAIERTVDERIDAMLAGGNTADLVNDFALPVPSLVISALLGVPSADRDFFESRTRTLVAI
RTSTDEERAEATRQLLRYINRLIVIKKKWRGEDLISRLLSTGKLSDEELSGVLLLLLIAG
HETTANNIGLGVVTLLSHREWIGDDRLVEELLRLHSVADMVALRVAVDDVEIAGQTIRKG
EGIVPLLASANHDTEAFGCPHAFNPERTERRHVAFGYGVHQCLGQNLVRVEMEIAYRKLF
ERIPELRLAVPEDQLAYKYDGILFGLHELPVRW
selected fasta
>cytochrome P450 [FscP]
ATGACGACCAGCCCCGGCCCGACGGTGGTCGACTTCCCACGGCGCACGCCCCGGGAACCG
CTGCCGCTGTCCCAGTACGCCGAACACCGGAAGCAGAACGGCCTGGTCCAGACGCACCTG
CCGAACGGCCGGCCGATCTGGCTGGTGACCCGGCACGAGGACGTGCGCGCGGTCCTCACC
CATCCCCGGATCAGCGCCAACCCCGACAACGAGGGCTTCCCCAACGTCGGCGAGACGATG
GGGGTGCCCAAGCAGGAGCAGATCCCCGGCTGGTTCGTCGGTCTGGACTCCCCCGAGCAC
GACCGTTTCCGCAAGGTGCTGATCCCCGAGTTCACCGTGCGCCGCGTCCGTGAGCTGCGG
CCGGCGATCGAACGGACCGTGGACGAGCGGATCGACGCGATGCTGGCCGGCGGGAACACC
GCGGACCTGGTGAACGACTTCGCGCTGCCCGTGCCGTCCCTGGTCATCTCCGCGCTGCTG
GGGGTGCCGTCGGCGGACCGGGACTTCTTCGAGTCCCGCACCCGCACGCTGGTCGCCATC
CGCACCTCGACCGACGAGGAGCGCGCCGAGGCCACCCGGCAGCTCCTGCGGTACATCAAC
CGGCTCATCGTCATCAAGAAGAAGTGGCGCGGCGAGGACCTCATCAGCCGCCTGCTGTCG
ACCGGCAAGCTCTCGGACGAGGAGCTGTCGGGCGTCCTGCTGCTCCTGCTGATCGCGGGC
CACGAGACCACGGCGAACAACATCGGGCTCGGCGTCGTCACGCTGCTCTCGCACCGGGAG
TGGATCGGCGACGACCGGCTCGTGGAGGAGCTGCTGCGCCTGCACTCGGTGGCCGACATG
GTGGCGCTGCGCGTCGCCGTGGACGACGTGGAGATCGCCGGCCAGACCATCCGCAAGGGC
GAGGGCATCGTGCCGCTGCTCGCCTCCGCCAACCACGACACCGAGGCGTTCGGCTGCCCG
CACGCCTTCAACCCGGAGCGCACCGAGCGCCGTCACGTGGCGTTCGGCTACGGCGTCCAC
CAGTGCCTGGGCCAGAACCTGGTCCGGGTCGAGATGGAGATCGCCTACCGCAAGCTCTTC
GAGCGCATCCCGGAGCTGCGGCTGGCCGTGCCCGAGGACCAGCTCGCCTACAAGTACGAC
GGGATCCTGTTCGGGCTGCACGAGCTGCCGGTGCGCTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[239-256] 6.7e-08 PR00385 [266-277] 6.7e-08 PR00385 [333-342] 6.7e-08 PR00385 [342-353] 6.7e-08 PR00385
PR00385   P450
[26-260] 3.7e-05 PF00067 [267-362] 7.5e-19 PF00067
PF00067   p450
[13-393] 3.6e-88 SSF48264
SSF48264   Cytochrome P450
[17-393] 7.8e-96 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
IPR002397 Cytochrome P450, B-class (Family)
[95-106] 7.5e-44 PR00359 [142-158] 7.5e-44 PR00359 [159-174] 7.5e-44 PR00359 [197-219] 7.5e-44 PR00359 [284-311] 7.5e-44 PR00359 [312-327] 7.5e-44 PR00359 [333-342] 7.5e-44 PR00359 [342-353] 7.5e-44 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[335-344] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
FR008_00080 FR008_00080   FR008_00100 FR008_00100
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