FR008_00070 FR008_00070   FR008_00090 FR008_00090

FR008_00080 : CDS information

close this sectionLocation

Organism
StrainFR-008
Entry nameFR-008
Contig
Start / Stop / Direction14,953 / 16,011 / + [in whole cluster]
14,953 / 16,011 / + [in contig]
Location14953..16011 [in whole cluster]
14953..16011 [in contig]
TypeCDS
Length1,059 bp (352 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductGDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase
Product (GenBank)FscMII
Gene
Gene (GenBank)fscMII
EC number2.6.1.-
Keyword
  • mycosamine
Note
Note (GenBank)
  • putative aminotransferase responsible for mycosamine biosynthesis
Reference
ACC
PmId
[14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003)
comment
complete FR-008 gene clusterの報告。
fscMII: GDP-ketosugar aminotransferase
@mycosamine biosynthesis

fscMII破壊mutantではsugar moietyを持たないFR-008 aglyconesが主に産生された。
そして低レベルではあるが、3-keto-6-deoxy-D-mannoseをsugar moietyとするものも得られた。
sugar moietyを持たないものが主なのはmycosamine glycosylTFの基質特異性が厳しいため。
Related Reference
ACC
Q93NX0
NITE
Amph_00130
PmId
[16079135] Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups. (J Biol Chem. , 2005)
comment
3rd(Q93NX0) 82%, 1e-172
Streptomyces nodosus_amphDII
[Amph_00130]sugar aminotransferase

amphDII geneがStreptomyces nodosusで削除されたとき、amphotericin aglyconeにC3でamino基を持たない(代わりにOH基を持つ)neutral deoxyhexoseの付着を招いた。

close this sectionSequence

selected fasta
>GDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase [FscMII]
MAFTHPVSRPALDGRELEYVSDAVSGGWISSQGPYVRRFEEAFAEWNGVAHGVACSSGTA
ALTLALRALNIGPGDEVIVPEFTMVASAWAVTYTGATPVFVDCGDDLNIDVTRIEEKITP
RTRAVMPVHVYGRRCDMDAVMDLALQYNLRVVEDSAEAHGVRPVGDIACFSLFANKIITA
GEGGVCLTDDPRLAEQLAHLRAMAFTRDHSFLHKKLAYNYRMTAMQGAVALAQTERLDEI
LATRREIEARYDAGLKDLPGITLMPARDVLWMYDLRAERREELRAHLDARGIETRLFFKP
MSRQPGYLDPVWPTLNAHRFSEDGLYLPTHTGLTAADQEYITGAVRDFYRAG
selected fasta
>GDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase [FscMII]
ATGGCCTTCACCCATCCCGTCTCCCGGCCCGCTCTGGACGGCCGGGAACTGGAGTACGTC
TCCGACGCCGTCTCCGGCGGCTGGATCTCCTCCCAGGGACCGTACGTCCGGCGTTTCGAG
GAAGCCTTCGCCGAGTGGAACGGCGTGGCGCACGGGGTGGCCTGCTCCTCCGGCACCGCC
GCCCTCACCCTGGCGCTGCGCGCGCTGAACATCGGCCCGGGTGACGAGGTGATCGTGCCG
GAGTTCACGATGGTCGCCTCCGCGTGGGCGGTGACCTACACCGGCGCCACCCCGGTCTTC
GTGGACTGCGGCGACGACCTGAACATCGACGTCACCCGCATCGAGGAGAAGATCACTCCG
CGCACCCGGGCGGTCATGCCGGTGCACGTCTACGGCCGGCGCTGCGACATGGACGCCGTG
ATGGACCTCGCCCTGCAGTACAACCTGCGGGTGGTCGAGGACTCGGCGGAGGCGCACGGG
GTCCGCCCGGTCGGCGACATCGCCTGCTTCTCGCTCTTCGCCAACAAGATCATCACGGCC
GGCGAGGGAGGCGTCTGCCTCACCGACGATCCCCGGCTCGCCGAACAGCTGGCCCATCTG
CGGGCGATGGCCTTCACCCGCGACCACAGCTTCCTGCACAAGAAACTGGCCTACAACTAC
CGGATGACGGCCATGCAGGGCGCCGTCGCCCTGGCCCAGACCGAGCGGCTCGACGAGATC
CTCGCCACCCGCCGGGAGATCGAGGCCCGCTACGACGCGGGGCTCAAGGACCTCCCCGGC
ATCACCCTGATGCCTGCCCGCGACGTCCTGTGGATGTACGACCTGCGTGCCGAGCGCCGC
GAGGAACTCCGCGCCCACCTGGACGCACGCGGCATCGAGACCCGGCTGTTCTTCAAGCCG
ATGAGCCGCCAGCCCGGCTACCTCGACCCGGTGTGGCCGACGCTCAACGCCCACCGGTTC
AGCGAGGACGGCCTGTACCTGCCCACCCACACCGGGCTGACCGCCGCCGACCAGGAGTAC
ATCACCGGCGCCGTCCGGGACTTCTACCGCGCGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[1-352] 2.2999842048857e-100 PIRSF000390
PIRSF000390   PLP_StrS
[12-346] 2.09999999999996e-104 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[6-234] 2.20000000000002e-78 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[237-350] 2.6e-22 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[2-349] 4.50001022902825e-102 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
FR008_00070 FR008_00070   FR008_00090 FR008_00090
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