FR008_00140 : CDS information

Location

Organism	Streptomyces sp.
Strain	FR-008
Entry name	FR-008
Contig	AY310323
Start / Stop / Direction	26,333 / 27,340 / + [in whole cluster] 26,333 / 27,340 / + [in contig]
Location	26333..27340 [in whole cluster] 26333..27340 [in contig]
Type	CDS
Length	1,008 bp (335 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter ATP-binding protein
Product (GenBank)	FscTI
Gene
Gene (GenBank)	fscTI
EC number
Keyword
Note
Note (GenBank)	putative ABC transporter
Reference	ACC Q6W5Q2 PmId [14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003) comment complete FR-008 gene clusterの報告。 fscTI: ABC transporter @efflux of FR-008 fscTI and fscTIIの推定産物は、S. coelicolor A3(2)のATP-dependent ABC transporter superfamilyに属するタンパクに似ており、たぶんFR-008の排出に関与している。
Related Reference	ACC Q9F5K4 NITE Avil_00350 PmId [11181344] An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. (Antimicrob Agents Chemother. , 2001) comment 18th(Q9F5K4) 51%, 3e-74 Streptomyces viridochromogenes_aviABC1 Putative ATP binding protein S. lividans TK66にてaviABC1, aviABC2, aviRa, aviRb発現。 --> avilamycin resistance (avilamycin A inhibited poly(U)-directed polyphenylalanine synthesis)
Related Reference	ACC Q53716 PmId [8326867] Streptomyces antibioticus contains at least three oleandomycin-resistance determinants, one of which shows similarity with proteins of the ABC-transporter superfamily. (Mol Microbiol. , 1993) comment 134th(Q53716) 50%, 2e-68 Streptomyces antibioticus_oleC-ORF4 ATP-binding protein S. antibioticsでoleandomycinに抵抗性を示す遺伝子を単離。 OleC plasmidでクローニングされたORFが5つあり、 Orf4 (NBD), Orf5(TMD)がABC transorterとして働いているのではないかと推論。

Sequence

>putative drug resistance ABC transporter ATP-binding protein [FscTI]
MNGLHLPPAPLPAPGVSAAGSGDLMIEAEGVSKAYGTVHALDSVSLAVPRGTVLGLLGHN
GAGKTTLVDILTTALPPTSGRARVAGYDVADKPVEIRRRIGLTGQFASVDAQLSGYDNLV
LIARLLGAGRRAARVRARELLELFRLTEVADRRASSYSGGLRRRLDLAVSLVGAPEVLFL
DEPTTGLDPSSRINLWEIVEGLVEQGTTVLLTTQYLEEADRLADRIAVLSAGRVVAAGTA
PELKATVGRRTVTLTLETGEDVSAARTALRGAGFAPVDGEARTVVVPIDATREIADIIRS
LDQAGVEASELNFGEPTLDDVYLTLAEQAAGRTRA

>putative drug resistance ABC transporter ATP-binding protein [FscTI]
ATGAACGGGCTCCACCTGCCCCCCGCCCCCTTGCCCGCCCCGGGCGTGAGCGCCGCCGGT
TCCGGCGATCTGATGATCGAGGCGGAGGGCGTCAGCAAGGCATACGGCACGGTCCACGCC
CTGGACTCGGTCAGCCTCGCCGTCCCCCGGGGCACCGTGCTGGGACTCCTCGGCCACAAC
GGCGCCGGGAAAACCACCCTGGTCGACATCCTCACCACGGCGCTGCCGCCCACCTCGGGG
CGGGCGAGGGTCGCCGGGTACGACGTGGCCGACAAGCCGGTCGAGATCCGCCGGCGCATC
GGGCTCACCGGGCAGTTCGCCTCGGTGGACGCGCAGCTCAGCGGGTACGACAACCTCGTC
CTGATCGCCCGTCTGCTGGGCGCCGGGCGCCGGGCGGCCCGCGTGCGCGCGAGGGAGCTG
CTGGAGCTGTTCCGGCTGACGGAGGTCGCGGACCGGCGTGCGAGCAGCTACTCGGGCGGG
CTGCGGCGGCGCCTGGACCTGGCCGTGTCGCTGGTCGGCGCGCCGGAGGTGCTCTTCCTC
GACGAGCCCACCACCGGCCTGGACCCCTCCAGCCGCATCAACCTGTGGGAGATCGTCGAG
GGGCTGGTCGAGCAGGGCACCACGGTGCTGCTGACCACGCAGTACCTGGAGGAGGCCGAC
CGGCTGGCGGACCGGATCGCGGTGCTCTCCGCGGGCCGGGTCGTCGCGGCGGGCACGGCG
CCCGAGCTGAAGGCCACCGTGGGGCGCCGCACGGTGACGCTCACCCTGGAGACCGGCGAG
GACGTGAGCGCGGCGCGCACGGCGCTGCGCGGGGCGGGCTTCGCCCCGGTCGACGGCGAG
GCCCGCACCGTGGTCGTCCCGATCGACGCCACACGGGAGATCGCCGACATCATCAGGAGC
CTGGACCAGGCCGGCGTCGAGGCGAGCGAGCTGAACTTCGGCGAACCCACGCTCGACGAC
GTGTACCTGACCCTCGCCGAGCAGGCCGCCGGCCGGACCCGGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003439 ABC transporter-like (Domain) PF00005 ABC_tran PS50893 ABC_TRANSPORTER_2 IPR003593 AAA+ ATPase domain (Domain) SM00382 ATPases associated with a variety of cellula IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family) TIGR01188 drrA: daunorubicin resistance ABC transporter, ATP
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

FR008_00130 FR008_00150