FR008_00150 : CDS information

Location

Organism	Streptomyces sp.
Strain	FR-008
Entry name	FR-008
Contig	AY310323
Start / Stop / Direction	27,561 / 28,280 / + [in whole cluster] 27,561 / 28,280 / + [in contig]
Location	27561..28280 [in whole cluster] 27561..28280 [in contig]
Type	CDS
Length	720 bp (239 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter permease protein
Product (GenBank)	FscTII
Gene
Gene (GenBank)	fscTII
EC number
Keyword
Note
Note (GenBank)	putative ABC transporter
Reference	ACC Q6W5Q1 PmId [14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003) comment complete FR-008 gene clusterの報告。 fscTII: ABC transporter @efflux of FR-008 fscTI and fscTIIの推定産物は、S. coelicolor A3(2)のATP-dependent ABC transporter superfamilyに属するタンパクに似ており、たぶんFR-008の排出に関与している。
Related Reference	ACC O86917 PmId [9696745] A silent ABC transporter isolated from Streptomyces rochei F20 induces multidrug resistance. (J Bacteriol. , 1998) comment 91st(O86917) 32%, 7e-17 Streptomyces rochei_ORF2 ORF2 protein Streptomyces rochei F20で、Msr-ORF1とMsr-ORF2は、ABC transporter superfamily のメンバーであるATP-binding proteinとtransmembrane proteinに各々似ていた。msrを破壊しても形質変化は認められず、行った実験条件では発現が検出されず、inducerの発見には至っていない。msrのプロモーター領域をexpressing promoterに入れ替えたプラスミドを作成し、Streptomyces lividans TK21に形質転換させると、薬剤や化学物質(doxorubicin, oleandomycin, erythromycin, tetracycline, spiramycin, streptothricin, tobramycin, novomycin, streptomycin, chloramphenicol, phenol, pentachlorophenol, tetrachlorohydroquinone, ethidium bromide)に抵抗性を示した。
Related Reference	ACC Q53717 PmId [8326867] Streptomyces antibioticus contains at least three oleandomycin-resistance determinants, one of which shows similarity with proteins of the ABC-transporter superfamily. (Mol Microbiol. , 1993) comment 139th(Q53717) 29%, 3e-15 Streptomyces antibioticus_oleC-ORF5 Membrane protein S. antibioticsでoleandomycinに抵抗性を示す遺伝子を単離。 OleC plasmidでクローニングされたORFが5つあり、 Orf4(NBD), Orf5(TMD)がABC transorterとして働いているのではないかと推論。 Orf5(TMD)については後にTopologyの解析もしてABC transporterのPermeaseであるといっている。

Sequence

>putative drug resistance ABC transporter permease protein [FscTII]
MVTDPGIVLFGLIQPVVILFVLTQVFSKMGMPPHFPDGVSYLDYVLPAVLVDNAAQSAMQ
SGVGLVEDQKNGIVARLRSLPVHPGALLAARSLVGLVRSAVQVAVIMALAMTVLGYSPNG
GAAELALSAGLTLFISFSLGWAFIAAGAWLRRAEPLQNLALIVIFPLMFASSAYVPVADL
PSWLGAVASVNPLTYAIDATRALALDVPGLDHAIPAVVICAVIAVVGMIAAVRGFRRPL

>putative drug resistance ABC transporter permease protein [FscTII]
ATGGTGACCGACCCGGGCATCGTGCTCTTCGGCCTGATCCAGCCGGTGGTCATCCTCTTC
GTGCTGACCCAGGTCTTCAGCAAGATGGGTATGCCGCCCCACTTCCCGGACGGCGTCAGC
TACCTCGACTACGTCCTGCCCGCCGTCCTGGTGGACAACGCCGCCCAGTCGGCGATGCAG
TCGGGCGTGGGCCTGGTGGAGGACCAGAAGAACGGCATCGTGGCCCGGCTGCGCTCCCTG
CCCGTCCACCCGGGCGCGCTGCTGGCCGCCCGCAGCCTGGTCGGCCTGGTGCGCAGCGCC
GTGCAGGTCGCGGTCATCATGGCGCTGGCGATGACCGTCCTCGGCTACTCCCCCAACGGC
GGTGCCGCCGAACTGGCCCTCTCCGCGGGGCTGACGCTCTTCATCAGCTTCTCGCTGGGC
TGGGCGTTCATCGCGGCCGGCGCCTGGCTGCGGCGGGCCGAGCCGCTGCAGAACCTCGCC
CTGATCGTGATCTTCCCGCTGATGTTCGCCTCCAGCGCCTACGTCCCGGTCGCGGACCTG
CCCTCCTGGCTGGGCGCGGTCGCGAGCGTCAACCCCCTGACGTACGCGATCGACGCGACC
CGGGCACTGGCGCTGGACGTGCCCGGCCTGGACCACGCGATCCCGGCGGTGGTGATCTGC
GCCGTGATCGCGGTGGTCGGCATGATCGCCGCCGTACGCGGCTTCCGCCGGCCTCTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000412 ABC-2 (Family) PS51012 ABC_TM2 IPR013525 ABC-2 type transporter (Domain) PF01061 ABC2_membrane IPR013526 ABC-2 transporter (Family) PR00164 ABC2TRNSPORT
SignalP	Bacteria, Gram-positive Eukaryota
TMHMM	Transmembrane 5

FR008_00140 FR008_00160