Megalo_00010 : CDS information

Location

Organism	Micromonospora megalomicea subsp. nigra
Strain	NRRL 3275 (=NBRC 14744)
Entry name	Megalomicin
Contig	AF263245
Start / Stop / Direction	144 / 1 / - [in whole cluster] 144 / 1 / - [in contig]
Location	complement(1..144) [in whole cluster] complement(1..144) [in contig]
Type	CDS
Length	144 bp (47 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase(fragment)
Product (GenBank)	TDP-4-keto-6-deoxyglucose-2,3-dehydratase
Gene	megBVI
Gene (GenBank)	megT
EC number
Keyword	megosamine mycarose
Note
Note (GenBank)
Reference	ACC Q9F841 PmId [10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000) [15870344] Production of the potent antibacterial polyketide erythromycin C in Escherichia coli. (Appl Environ Microbiol. , 2005) [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010) comment [PMID: 10972798](2000) megalomicin (meg) biosynthetic gene clusterの報告。 megT: 2,3-dehydratase megTはdeoxysugar生合成に関与すると提唱。 TDP-L-megosamineの前駆体であるdTDP-L-daunosamineの生成について述べられている[PMID: 10631513]を参照して割り当て。 dnmT, dnmU, dnmJ and dnmVがmeg clusterのmegT, megDIV, megDII and megDVに相当。 --- ■for mycarose biosynthesis [PMID: 15870344](2005) G-1-P → dTDP-L-mycaroseの変換に必要な7つの遺伝子(megDIV, BII, M, L, BVI, BIV, BIII)とmycarosyltransferase_megBV、C-6 hydroxylase_megFを含んだmycarose operon(pKOS452-53e)を構築し、E.coliで発現。LC/MS解析により、6-deoxyerythronolide Bから3-mycarosyl-erythronolide Bが得られることを確認。 --- ■for megosamine biosynthesis [PMID: 20418422](2010) 異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS analysis。 dTDP-4-keto-6-deoxy-D-glucoseからの、MegBVI, MegDII, MegDIIIによる3stepsが確立された。

Sequence

>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase(fragment) [TDP-4-keto-6-deoxyglucose-2,3-dehydratase]
MGDRVNGHATPESTQSAIRFLTRHGGPPTATDDVHDWLAHRAAEHRLE

>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase(fragment) [TDP-4-keto-6-deoxyglucose-2,3-dehydratase]
ATGGGGGATCGGGTCAACGGTCATGCGACTCCGGAATCCACACAATCGGCAATACGTTTC
CTGACTCGGCACGGCGGACCTCCCACCGCCACCGACGACGTCCACGACTGGTTGGCCCAC
CGCGCCGCCGAGCATCGGCTCGAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

first Megalo_00020