Monen_00010 Monen_00010   Monen_00030 Monen_00030

Monen_00020 : CDS information

close this sectionLocation

Organism
StrainATCC 15413
Entry nameMonensin
Contig
Start / Stop / Direction2,140 / 1,220 / - [in whole cluster]
2,140 / 1,220 / - [in contig]
Locationcomplement(1220..2140) [in whole cluster]
complement(1220..2140) [in contig]
TypeCDS
Length921 bp (306 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative dihydrodipicolinate synthase
Product (GenBank)dihidro-dipicolinate synthase
Gene
Gene (GenBank)dapA
EC number4.2.1.52
Keyword
Note
Note (GenBank)
  • similar to dihidro-dipicolinate synthase
Reference
ACC
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
comment
monensin生合成 gene clusterの解析論文。

DapA: Dihydrodipicolinate synthase

clusterの両脇に見られた一次代謝関連酵素のひとつ。
本文中に詳細記載なし。
Related Reference
ACC
P0A6L2
PmId
[1463470] ( , )
[15158272] ( , )
comment
Blast 164th, id32%, 3e-33
E.coli_dapA
4-hydroxy-tetrahydrodipicolinate synthase(EC 4.3.3.7)

diaminopimelic acidを制限した状態にするとそれに応答してdapAの発現が増えることを確認している。
また、構造解析の結果から活性部位を明らかにしている。

close this sectionSequence

selected fasta
>putative dihydrodipicolinate synthase [dihidro-dipicolinate synthase]
MTLASSLEPTTEPLFNGLYVPLVTPFTDDLRLAPEALARLADEALSAGASGLVALGTTAE
AATLTAEERETVIRVCSAACRAHGAPLIVGVGTNDTATAITALRELAARGDVAAALVPAP
PYIRPGEAGTLAHFAALAEHGGLPLVVYDIPYRTGQTLGAGTITALGRLPEVVGIKHATG
SIDPTTMELLDSPLPGFAVLGGDDIVLSPLVAAGAHGGIVASANLRTADYAEMIALWRRG
SAAPARALGADLARLSAALFTEPNPTVIKGVLHAQNRIPSPAVRMPLLAASADSVRRAAP
LAASRK
selected fasta
>putative dihydrodipicolinate synthase [dihidro-dipicolinate synthase]
ATGACCCTCGCATCGAGCCTCGAACCGACCACCGAACCGCTCTTCAACGGCCTGTACGTC
CCCCTGGTAACTCCGTTCACCGACGATCTGCGCCTGGCCCCCGAGGCGCTGGCCCGACTC
GCCGACGAGGCGCTGTCGGCCGGCGCCTCCGGGCTCGTCGCCCTCGGTACGACCGCCGAG
GCCGCCACACTGACCGCCGAGGAGAGAGAGACGGTGATCCGCGTCTGCTCGGCCGCGTGC
CGGGCACACGGGGCACCGCTCATCGTCGGGGTGGGCACCAACGACACCGCCACCGCCATC
ACGGCCCTGCGAGAGCTGGCGGCCCGGGGTGACGTCGCCGCCGCGCTGGTCCCCGCGCCG
CCCTACATCCGCCCCGGGGAAGCGGGGACCCTGGCTCATTTCGCCGCGCTGGCCGAGCAC
GGCGGCCTCCCCCTGGTCGTGTACGACATCCCTTACCGCACCGGGCAGACGCTCGGCGCG
GGCACGATCACCGCACTCGGCCGCCTGCCGGAGGTCGTCGGCATCAAGCACGCGACCGGC
TCGATCGACCCGACCACCATGGAGTTGCTCGACTCGCCCCTGCCCGGCTTCGCCGTGCTC
GGCGGTGACGACATCGTCCTCTCGCCGCTGGTCGCTGCGGGCGCCCACGGCGGAATCGTG
GCGTCGGCCAATCTGCGTACCGCCGACTACGCCGAGATGATCGCGCTGTGGCGACGCGGC
TCCGCCGCACCCGCCCGCGCTCTCGGAGCCGACCTCGCCCGCCTTTCCGCCGCCCTCTTC
ACCGAACCGAACCCGACGGTGATCAAGGGAGTGCTGCACGCCCAGAACCGCATTCCCAGC
CCGGCCGTCCGCATGCCACTGCTGGCCGCCTCCGCCGATTCGGTCCGCCGGGCGGCACCC
CTGGCCGCGAGCCGGAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002220 Dihydrodipicolinate synthetase-like (Family)
[12-304] 5.10003242202156e-90 PIRSF001365
PIRSF001365   DHDPS
[48-69] 4.60000044330862e-20 PR00146 [85-103] 4.60000044330862e-20 PR00146 [118-134] 4.60000044330862e-20 PR00146 [143-160] 4.60000044330862e-20 PR00146
PR00146   DHPICSNTHASE
[15-293] 1.80000000000002e-55 PF00701
PF00701   DHDPS
IPR013785 Aldolase-type TIM barrel (Domain)
[14-298] 7.5e-74 G3DSA:3.20.20.70
G3DSA:3.20.20.70   Aldolase_TIM
IPR020625 Dihydrodipicolinate synthetase, active site (Active_site)
[148-178] PS00666
PS00666   DHDPS_2
SignalP No significant hit
TMHMM No significant hit
Monen_00010 Monen_00010   Monen_00030 Monen_00030
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