Monen_00090 Monen_00090   Monen_00110 Monen_00110

Monen_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 15413
Entry nameMonensin
Contig
Start / Stop / Direction9,542 / 8,643 / - [in whole cluster]
9,542 / 8,643 / - [in contig]
Locationcomplement(8643..9542) [in whole cluster]
complement(8643..9542) [in contig]
TypeCDS
Length900 bp (299 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productthioesterase
Product (GenBank)monensin cyclase
Gene
Gene (GenBank)monCII
EC number
Keyword
  • unusual type
Note
Note (GenBank)
  • monensin epoxide hydrolase; MonCII
Reference
ACC
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
[16897798] Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin. (Chembiochem. , 2006)
comment
[PMID: 12940979](2003)
monensin生合成 gene clusterの解析論文。

MonCII: Cyclase
monCII のTargeted gene replacementによってmonensin非産生。

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[PMID: 16897798](2006) abstract
epoxide hydrolaseであると考えられてきたが、alpha/beta-hydrolase structural familyに属する新しいthioesteraseである。

精製されたrecombinant MonCII は、monensin AのN-acetylcysteamine thioester derivativeを含むいくつかのthioester基質を加水分解する。
phenylmethanesulfonyl fluorideのような酵素のhallmark inhibitorとのincubationは、thioesterase活性の抑制と、MonCII のacylated formの蓄積を導く。

close this sectionSequence

selected fasta
>thioesterase [monensin cyclase]
MKNLRIPVSQTVSLNVRYRPADGPGAPGRPFLLLHGMLSNARMWDEVAARLAAAGHPAYA
VDHRGHGESDTPPDGYDNATVVTDLVAAVTALDLSGALVAGHSWGAHLALRLAAEHPDLV
AGLALIDGGWYEFDGPVMRAFWERTADVVRRAQQGTTSAADMRAYLRATHPDWSPTSIEA
RLADYRVGPDGLLIPRLTSTQVMSIVAGLQREAPADWYPKVTVPVRLLPLIPAIPQLSDQ
VRAWVAAAEAALEQVSVRWYPGSDHDLHAGAPDEIAADLLLLARSCEAMPGGKAGVRPA
selected fasta
>thioesterase [monensin cyclase]
GTGAAGAACCTCCGCATCCCCGTGTCGCAGACCGTCTCCCTCAATGTCAGGTACCGGCCC
GCGGACGGGCCGGGAGCCCCGGGCCGTCCGTTCCTGCTGCTGCACGGCATGCTGTCGAAC
GCCCGGATGTGGGACGAGGTCGCCGCCCGTCTCGCCGCCGCCGGACACCCGGCCTACGCC
GTCGACCACCGCGGCCACGGCGAGTCGGACACCCCGCCCGACGGGTACGACAACGCGACG
GTCGTCACCGACCTGGTGGCGGCCGTCACCGCCCTCGACCTGTCCGGCGCACTGGTGGCC
GGGCACTCCTGGGGCGCGCACCTCGCGCTGCGCCTGGCGGCCGAGCACCCGGACCTGGTG
GCCGGTCTCGCCCTGATCGACGGCGGCTGGTACGAGTTCGACGGACCGGTGATGCGCGCC
TTCTGGGAGCGCACCGCCGACGTGGTCCGGCGCGCCCAGCAGGGCACCACCAGCGCCGCC
GACATGCGCGCCTACCTGCGGGCCACCCATCCCGACTGGTCGCCCACGTCCATCGAGGCA
CGCCTGGCGGACTACCGCGTCGGGCCGGACGGCCTGCTCATCCCCCGCCTGACCTCGACA
CAGGTCATGTCGATCGTCGCCGGCCTGCAACGGGAGGCACCGGCCGACTGGTATCCCAAG
GTCACCGTGCCGGTGCGGCTGCTGCCCCTCATCCCCGCCATCCCCCAACTGTCCGACCAG
GTACGGGCCTGGGTGGCCGCCGCCGAGGCGGCCCTGGAGCAGGTGAGCGTGCGCTGGTAC
CCGGGCTCCGACCACGATCTGCACGCCGGCGCACCCGACGAGATCGCCGCCGACCTCCTG
CTGCTGGCCCGCTCGTGCGAGGCGATGCCCGGCGGCAAGGCGGGAGTTAGACCCGCTTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Monen_00090 Monen_00090   Monen_00110 Monen_00110
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