Monen_00220 : CDS information

Location

Organism	Streptomyces cinnamonensis
Strain	ATCC 15413
Entry name	Monensin
Contig	AF440781
Start / Stop / Direction	76,541 / 75,051 / - [in whole cluster] 76,541 / 75,051 / - [in contig]
Location	complement(75051..76541) [in whole cluster] complement(75051..76541) [in contig]
Type	CDS
Length	1,491 bp (496 aa)

Annotation

Category	3.3 modification reduction
Product	epoxidase
Product (GenBank)	monensin epoxidase
Gene	monCI
Gene (GenBank)	monC1
EC number
Keyword
Note
Note (GenBank)	FAD dependent monooxygenase; MonC1
Reference	ACC Q846W9 PmId [12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003) [16217816] Accumulation of an E,E,E-triene by the monensin-producing polyketide synthase when oxidative cyclization is blocked. (Angew Chem Int Ed Engl. , 2005) comment [PMID: 12940979](2003) monensin生合成 gene clusterの解析論文。 MonCI: Epoxidase monCIのS. coelicolorにおける異種性発現で、linaloolをepoxidizeする能力が10-20倍に増加することを確認している。 --- [PMID: 16217816](2005) monCI を削除されたmutantは、E-, E-, E-立体配置を持ったtriene shunt metaboliteを蓄積した。

Sequence

>epoxidase [monensin epoxidase]
MTTTRPAHAVVLGASMAGTLAAHVLARHVDAVTVVERDALPEEPQHRKGVPQARHAHLLW
SNGARLIEEMLPGTTDRLLAAGARRLGFPEDLVTLTGQGWQHRFPATQFALVASRPLLDL
TVRQQALGADNITVRQRTEAVELTGSGGGSGGRVTGVVVRDLDSGRQEQLEADLVIDATG
RGSRLKQWLAALGVPALEEDVVDAGVAYATRLFKAPPGATTHFPAVNIAADDRVREPGRF
GVVYPIEGGRWLATLSCTRGAQLPTHEDEFIPFAENLNHPILADLLRDAEPLTPVFGSRS
GANRRLYPERLEQWPDGLLVIGDSLTAFNPIYGHGMSSAARCATTIDREFERSVQEGTGS
ARAGTRALQKAIGAAVDDPWILAATKDIDYVNCRVSATDPRLIGVDTEQRLRFAEAITAA
SIRSPKASEIVTDVMSLNAPQAELGSNRFLMAMRADERLPELTAPPFLPEELAVVGLDAA
TISPTPTPTPTAAVRS

>epoxidase [monensin epoxidase]
GTGACCACCACCCGACCCGCACATGCCGTCGTCCTGGGTGCCAGCATGGCAGGCACCCTG
GCGGCCCACGTCCTGGCCCGGCACGTCGACGCCGTCACCGTCGTGGAACGTGACGCGCTG
CCCGAGGAGCCCCAGCACCGCAAGGGGGTCCCGCAGGCCCGCCACGCGCACCTCCTGTGG
TCCAACGGCGCCCGCCTCATCGAGGAGATGCTGCCCGGCACCACCGACCGCCTCCTCGCG
GCCGGCGCCCGCCGCCTCGGCTTCCCCGAGGACCTGGTGACGCTGACCGGTCAGGGGTGG
CAGCACCGCTTCCCCGCCACCCAGTTCGCGCTGGTCGCCAGCCGCCCGCTGCTGGACTTG
ACGGTACGTCAGCAGGCCTTGGGAGCCGACAACATCACCGTCCGGCAGCGCACCGAGGCC
GTCGAGCTGACGGGAAGTGGTGGTGGCAGTGGCGGCCGGGTCACCGGTGTCGTCGTCCGC
GACCTCGACAGCGGTCGGCAGGAGCAGCTGGAGGCCGACCTGGTGATCGACGCCACCGGG
CGCGGCTCCCGCCTCAAGCAGTGGCTCGCCGCCCTCGGCGTGCCGGCACTGGAGGAAGAC
GTCGTGGACGCGGGCGTCGCCTACGCCACCCGGCTCTTCAAGGCCCCGCCGGGCGCTACG
ACGCACTTCCCCGCCGTGAACATCGCCGCCGACGACCGGGTGCGCGAACCCGGCCGCTTC
GGCGTGGTCTACCCCATCGAGGGCGGCCGCTGGCTCGCGACGCTCTCCTGCACGCGGGGC
GCGCAACTGCCCACACACGAGGACGAGTTCATCCCCTTCGCCGAGAACCTGAACCACCCG
ATCCTCGCCGACCTGCTCCGGGACGCCGAACCACTCACCCCCGTCTTCGGTTCCCGCTCC
GGCGCCAACCGCCGCCTCTATCCGGAGCGCCTCGAACAGTGGCCCGACGGTCTGCTCGTC
ATCGGCGACTCGCTGACCGCTTTCAACCCGATCTACGGCCACGGCATGAGCTCGGCCGCG
CGCTGCGCCACCACGATCGACCGCGAGTTCGAGCGGAGCGTGCAGGAGGGTACGGGGTCC
GCCCGCGCCGGCACCCGCGCCCTGCAGAAGGCGATCGGCGCGGCCGTGGACGACCCGTGG
ATCCTCGCCGCGACCAAGGACATCGACTACGTCAACTGCCGTGTCTCGGCGACGGATCCG
CGGCTCATCGGTGTGGACACCGAGCAGCGTCTGCGGTTCGCCGAGGCCATCACCGCGGCG
TCGATCCGTTCCCCGAAGGCGTCCGAGATCGTCACGGACGTGATGAGCCTCAACGCGCCG
CAGGCCGAACTGGGCTCCAACCGTTTCCTGATGGCGATGCGCGCCGACGAACGCCTCCCG
GAGCTGACCGCTCCCCCGTTCCTTCCGGAGGAGCTGGCCGTGGTGGGTCTGGACGCGGCG
ACGATCTCGCCCACGCCTACACCTACACCTACGGCGGCTGTACGGTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003953 FAD binding domain (Domain) PF00890 FAD_binding_2
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Monen_00210 Monen_00230