Monen_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 15413
Entry nameMonensin
Contig
Start / Stop / Direction72,051 / 74,993 / + [in whole cluster]
72,051 / 74,993 / + [in contig]
Location72051..74993 [in whole cluster]
72051..74993 [in contig]
TypeCDS
Length2,943 bp (980 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator
Product (GenBank)putative regulatory protein
Gene
Gene (GenBank)monH
EC number
Keyword
Note
Note (GenBank)
  • contains helix-turn-helix motif; similar to RapH; MonH
Reference
ACC
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
comment
monensin生合成 gene clusterの解析論文。

MonH: Transcriptional regulator

MonH はPKS clustersに見られるtranscriptional regulatorsのemerging familyに属する。
メンバーはN-terminal ATP-binding domainによって特徴付けられている。
Related Reference
ACC
Q9ZGI0
NITE
Pikro_00180
PmId
[11344155] Characterization and analysis of the PikD regulatory factor in the pikromycin biosynthetic pathway of Streptomyces venezuelae. (J Bacteriol. , 2001)
[18245260] Enhanced heterologous production of desosaminyl macrolides and their hydroxylated derivatives by overexpression of the pikD regulatory gene in Streptomyces venezuelae. (Appl Environ Microbiol. , 2008)
[26608164] Interspecies Complementation of the LuxR Family Pathway-Specific Regulator Involved in Macrolide Biosynthesis. (J Microbiol Biotechnol. , 2016)
comment
BLAST id35%
Streptomyces venezuelae_pikD
[Pikro_00180]transcriptional regulator

--
[PMID: 11344155](2001)
pikDは抗生物質産生に必須。pikD deletionによる抗生物質非産生は、plasmidによる相補で回復する。
PikDによって行使される調節のレベルを、pathway中間体の変換と、xylE reporter systemを使ったpromoter probingで見ている。
PikDが媒介する転写調節は、pikRII, pikAI, and desI の発現を調節するpromotersで起こるが、pikRI or pikCを調節するpromotersでは起こらない。

---
[PMID: 18245260](2008)
S.venezuelae ATCC 15439のPKS genes deletion mutantにtylosin PKS genesを導入した株と、さらにpikDの追加コピーを入れた株での産物解析。
pikD追加があるとdesosaminyl tylactone産生が増えるので、PikDはdesosamine生合成gene clusterの発現をupregulateすると示唆される。また、2つのhydroxylate型desosaminyl tylactoneが新たにこの株から検出され、P450 hydroxylaseをコードするpikCの発現がPikDによって増加したためであると考えられた。

RT-PCRによる遺伝子発現解析と、10-deoxymethynolide, narbonolide, and TL→対応するdesosaminyl macrolidesへの生物変換実験によっても、PikDがdes and pikC genesのpositive regulatorであることを確かめている。

[PMID: 11344155]との矛盾点はxylE assayでのせいであり、RT-PCRと生物変換実験を使った結果のほうが直接的な証拠であると言っている。

---
[PMID: 26608164](2016)
pikDの過剰発現はpikromycin産生を拡大し、pikD deletion mutantはrapHとfkbNで補完することができる。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator [putative regulatory protein]
MSGVERGVGSAGPVEQGDGLAGLVERAEALAALRGAFDGSPGTGGSLVVLSGAVGTGKTA
LLRAWADRIGADADALVLTATACRAERDLPLGVLEQLVRSPGLPPASAERALAWWDEEAS
ATPGKTDANGTSANGTDANGTGAGQTGAGQAGVGQTGVGGEPVLAASALRGLCEVLRDLL
AERPVVVAVDDAHHADAASLQCLLSVVRRLRSARLHVLFTEYAHQKAQNALLSSEFLHEP
ALRRIRLEPLSKAGVEALLARHLDERTAQDLTPVVHGMSAGHPLLVRALAEDHRAAGGAG
EAYGRAVLSFLYRHETPVTQVARAIAALGAHAGPGQVGRLLDVDAASVERAVRQLTVAEV
LHEGRLCHPAFAAAVLDGMPPEERRALHGRVADLLHEEGAPATEVAAHLVAADRSDAPWA
VPVFQEAAQLALDEDQVETGVDYLRAAHQRCRGAAQRAAVVGALADAEWRLDPAKVLRHL
PDPAAMAPQTDPAALAPHTDPAPTAAPTAAPTPTPIPTTPPLPTHLLWHGRVEEGLDAIG
TLTGPGPNPAGAPPMNPADLDTPWLWGAYLYPGHVKERLGSGALSPQRSTPPAVTPELQG
AGTLMNDLLHGGERDATEAAERALNRYRLGPRTIAVQTAALAALTYRDRPHRAAAWCDGL
VAQADERNSPTWRALFTAWRALLHLRQGDPAAAEQRAETALALLGSKGWGAAIGLPLAAA
VQAKAALGDVDGAAALLERPVPQAVFQTRTGLHYLAARGRYHLATGCHYAALCDFYACGT
RMSSWGVDLPALEPWRLGAAEAYLALGEGLLARQLVDGQLPLPTPDDGRTWGMTLRLRAA
TSPAPARAELLDEAVAVLRESGDTFELARAVADQAVAVREGGEAERARLLARKAELLARR
WGSAPAPATVPEPPERPGPATPDAELTSAERRVAELAAEGFTNREISRKLCVTVSTVEQH
LTRIYRKLDVRRLDLQAALG
selected fasta
>putative LuxR family transcriptional regulator [putative regulatory protein]
GTGAGCGGGGTGGAGCGGGGTGTGGGGTCGGCGGGCCCTGTGGAACAGGGTGACGGACTC
GCGGGCCTGGTCGAGCGGGCCGAGGCGCTGGCCGCTCTGCGGGGCGCCTTCGACGGCTCC
CCGGGCACCGGCGGCAGCCTCGTCGTGCTCAGCGGCGCGGTGGGCACCGGCAAGACCGCG
CTGCTACGGGCGTGGGCCGACCGCATCGGCGCCGATGCCGACGCCCTGGTCCTGACCGCC
ACCGCCTGCCGCGCCGAGCGCGACCTGCCGCTTGGCGTCCTGGAACAGCTGGTACGCAGC
CCCGGCCTGCCCCCGGCCAGCGCCGAGCGCGCGCTGGCGTGGTGGGACGAGGAGGCCTCG
GCCACCCCCGGAAAGACGGACGCGAACGGGACGAGTGCCAACGGGACGGACGCCAACGGG
ACGGGCGCGGGACAGACGGGCGCGGGGCAGGCGGGCGTGGGACAGACGGGCGTGGGCGGA
GAGCCCGTCCTGGCCGCCTCCGCCCTGCGAGGCCTGTGCGAGGTGCTGCGGGACCTGCTC
GCCGAGCGGCCCGTCGTGGTCGCCGTCGACGACGCGCACCATGCCGACGCGGCGTCGCTC
CAGTGCCTGCTCTCCGTGGTGCGCCGGCTGCGGTCGGCACGACTCCATGTGCTGTTCACC
GAGTACGCCCATCAGAAGGCGCAGAACGCCCTGCTGAGCAGCGAGTTCCTGCACGAGCCC
GCCCTGCGGCGGATCCGCCTGGAACCGCTGTCGAAGGCGGGCGTGGAGGCCTTGCTCGCC
CGGCACCTCGACGAGCGGACGGCACAAGACCTCACCCCCGTCGTCCACGGCATGAGCGCG
GGCCACCCGCTCCTCGTACGGGCGCTGGCCGAGGACCACCGTGCGGCGGGCGGCGCCGGG
GAGGCGTACGGTCGTGCCGTCCTCAGCTTTCTGTACCGGCACGAGACTCCGGTCACCCAA
GTCGCCCGCGCCATCGCTGCGTTGGGCGCGCACGCCGGACCCGGTCAGGTCGGGCGGCTG
CTCGATGTCGACGCGGCGTCCGTCGAGCGGGCCGTGCGGCAGCTGACCGTCGCGGAGGTG
CTGCACGAGGGCCGCCTGTGCCACCCGGCGTTCGCGGCGGCGGTCCTGGACGGCATGCCG
CCCGAGGAACGCCGCGCCCTGCACGGACGGGTCGCCGACCTCCTGCACGAGGAGGGGGCG
CCGGCCACCGAAGTGGCCGCCCACCTCGTCGCCGCCGACCGGTCCGACGCCCCGTGGGCG
GTACCCGTCTTCCAGGAAGCGGCCCAACTCGCCCTGGACGAGGACCAGGTGGAGACCGGC
GTCGACTATCTGCGCGCGGCCCACCAGCGGTGCCGGGGCGCCGCGCAGCGTGCCGCGGTC
GTCGGTGCGCTCGCCGACGCCGAGTGGCGGCTCGACCCAGCAAAGGTCCTGCGCCACCTG
CCCGACCCTGCAGCCATGGCCCCACAAACGGACCCTGCCGCCCTGGCCCCACACACGGAC
CCCGCACCCACAGCCGCACCCACAGCCGCCCCCACCCCCACCCCCATCCCGACCACCCCA
CCCCTCCCCACCCACCTGCTCTGGCACGGGCGGGTCGAGGAAGGCCTGGACGCCATCGGC
ACGCTCACCGGGCCCGGACCCAACCCGGCGGGTGCGCCGCCGATGAACCCCGCGGACCTG
GACACCCCATGGCTGTGGGGCGCCTACCTCTATCCCGGGCACGTCAAGGAGCGCCTGGGA
TCCGGCGCCCTGTCCCCGCAGCGCTCGACCCCGCCGGCGGTCACGCCGGAGCTCCAAGGC
GCGGGCACGCTGATGAACGACCTGCTGCACGGCGGCGAACGCGACGCCACCGAGGCCGCC
GAGCGCGCCCTCAACCGCTACCGGCTCGGCCCCCGCACCATCGCGGTCCAGACGGCCGCG
CTGGCCGCCCTCACCTACCGCGACCGGCCGCACCGCGCGGCCGCCTGGTGCGACGGCCTC
GTCGCCCAGGCCGACGAGCGCAACAGCCCCACCTGGCGGGCCCTGTTCACCGCGTGGCGT
GCCCTGCTCCACCTGCGGCAGGGCGACCCGGCCGCAGCGGAACAGCGCGCCGAAACCGCC
CTCGCCCTGCTCGGATCGAAGGGCTGGGGCGCCGCGATCGGCCTGCCGCTGGCAGCCGCC
GTACAGGCCAAGGCGGCCCTCGGCGATGTCGACGGGGCGGCGGCCCTCCTGGAACGGCCC
GTGCCCCAGGCGGTCTTCCAGACCCGCACCGGACTGCACTACCTGGCGGCCCGGGGCCGC
TATCACCTCGCCACCGGCTGCCACTACGCCGCACTGTGCGACTTCTACGCCTGCGGGACC
CGCATGAGCAGCTGGGGAGTGGACCTGCCCGCGCTGGAGCCGTGGCGCCTCGGCGCGGCG
GAAGCGTACCTGGCCCTCGGCGAAGGACTCCTGGCACGCCAACTCGTCGACGGCCAGCTG
CCGTTGCCCACGCCTGACGACGGCCGCACCTGGGGCATGACGTTGCGCCTGCGGGCGGCC
ACGTCCCCCGCGCCGGCCCGGGCCGAACTCCTCGACGAGGCCGTGGCGGTGCTCCGGGAG
AGCGGCGACACCTTCGAGCTGGCGCGGGCCGTCGCCGACCAGGCTGTTGCCGTACGCGAA
GGGGGCGAGGCGGAACGCGCCCGGCTGCTGGCCCGCAAGGCGGAGCTGCTGGCCCGGCGC
TGGGGCAGCGCCCCCGCGCCCGCCACCGTCCCCGAACCGCCGGAGCGGCCAGGACCGGCC
ACTCCGGACGCCGAACTGACCAGTGCGGAGCGGAGGGTGGCCGAGCTGGCCGCCGAAGGG
TTCACCAACCGGGAGATCTCCCGGAAGCTGTGCGTCACGGTCAGCACCGTGGAACAGCAC
CTGACCCGGATCTACCGGAAGCTCGACGTCAGGCGACTGGACCTCCAGGCAGCCCTCGGC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
 [926-940]  1e-08 PR00038 [940-956]  1e-08 PR00038 [956-968]  1e-08 PR00038
PR00038   HTHLUXR
 [923-979]  3.69999438558106e-18 SM00421
SM00421   HTH_LUXR
 [940-967]  PS00622
PS00622   HTH_LUXR_1
 [924-971]  9.8e-12 PF00196
PF00196   GerE
 [919-980]  PS50043
PS50043   HTH_LUXR_2
IPR003593 AAA+ ATPase domain (Domain)
 [44-251]  4.19999771339581e-06 SM00382
SM00382   AAA
IPR011990 Tetratricopeptide-like helical (Domain)
 [615-760]  2.2e-08 G3DSA:1.25.40.10 [761-936]  2.2e-08 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
 [937-972]  1.9e-15 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
 [904-971]  6.29999562859499e-15 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP
 [1-29]  0.183 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Page top