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Monen_00340 : CDS information

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Organism
StrainATCC 15413
Entry nameMonensin
Contig
Start / Stop / Direction102,924 / 103,450 / + [in whole cluster]
102,924 / 103,450 / + [in contig]
Location102924..103450 [in whole cluster]
102924..103450 [in contig]
TypeCDS
Length527 bp (175 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative amidinotransferase(fragment)
Product (GenBank)glycine amidinotransferase
Gene
Gene (GenBank)amtA
EC number
Keyword
Note
Note (GenBank)
  • AmtA
Reference
ACC
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
comment
monensin生合成 gene clusterの解析論文。

ORF34: L-arginine:lysine amidinotransferase
論文には詳細記載なし。
Related Reference
ACC
P50441
PmId
[8313955] The amino acid sequences of human and pig L-arginine:glycine amidinotransferase. (FEBS Lett. , 1994)
comment
27th(P50441) 44%, 2e-35, N末部分hit
Pig_GATM(AGAT)
Glycine amidinotransferase(EC 2.1.4.1)
synonym: L-arginine:glycine amidinotransferase)

ブタ肝臓のミトコンドリアからL-arginine:glycine amidinotransferaseのタンパク分離、AA配列解析。
タンパク分離の際に活性測定を利用している。

この配列の一部を利用してヒト酵素のcDNA cloneを分離、シークエンス。
bacterial L-arginine:inosamine phosphate amidinotransferases_strB1, strB2とalignment見ている。
ACC
P08078
PmId
[3118332] Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity. (Nucleic Acids Res. , 1987)
[9922132] Crystal structure of L-arginine:inosamine-phosphate amidinotransferase StrB1 from Streptomyces griseus: an enzyme involved in streptomycin biosynthesis. (Biochemistry. , 1998)
comment
55th(P08078) 45%, 5e-32, N末部分hit
Streptomyces griseus_strB1(strB)
Inosamine-phosphate amidinotransferase 1(EC 2.1.4.2)
synonym: Aminocyclitol amidinotransferase

---
[PMID:3118332](1987)
streptomycin生合成gene clusterでmajor resistance gene_aphDの周りにある3genesの同定。
aphDの下流にあるstrBはaminocyclitol amidinotransferase (ADT)をコードする。
機能的なstrR geneがないとADT活性なし。

---
[PMID:9922132](1998) abstract
Streptomyces griseus由来L-Arginine:inosamine-phosphate amidinotransferase StrB1の構造解析。

close this sectionSequence

selected fasta
>putative amidinotransferase(fragment) [glycine amidinotransferase]
MSPVNSHNEWDPLEEIIVGRLEGATIPSSHPVVACNIPTWAARLQGLAAGFEYPQRLIEP
AQQELDQFIALLQSLDVTVRRPAAVDHKHRFGTPDWQSRGFCNSCPRDSMLVVGDEIIET
PMAWPCRCFETHSYRELLKDYFRRGARWTAAPRPQLTEALYEKDFRPPEEGERCA-
selected fasta
>putative amidinotransferase(fragment) [glycine amidinotransferase]
ATGTCACCCGTCAATTCGCACAACGAGTGGGATCCGCTGGAGGAGATCATCGTCGGGCGG
CTGGAGGGCGCGACCATTCCCTCCAGCCATCCGGTCGTGGCGTGCAACATCCCGACCTGG
GCGGCACGGCTGCAGGGTCTCGCCGCCGGGTTCGAGTATCCGCAGCGGCTGATCGAGCCG
GCGCAGCAGGAGCTCGACCAGTTCATCGCTCTCCTGCAATCCCTCGACGTCACAGTGAGA
CGGCCGGCGGCCGTCGACCACAAGCACCGCTTCGGGACCCCCGACTGGCAGTCGCGCGGC
TTCTGCAATTCCTGTCCGCGGGACAGCATGCTCGTCGTCGGCGACGAGATCATCGAGACC
CCGATGGCGTGGCCGTGCCGCTGTTTCGAGACGCACTCGTACCGCGAACTCCTCAAGGAC
TACTTCCGGCGCGGCGCGCGCTGGACGGCGGCGCCGCGCCCCCAGCTCACCGAGGCCCTG
TACGAGAAGGACTTCCGCCCTCCCGAGGAGGGCGAACGATGCGCTAC

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-41] 0.052 Signal
Eukaryota   
[1-34] 0.067 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
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