Monen_00330 : CDS information

Location

Organism	Streptomyces cinnamonensis
Strain	ATCC 15413
Entry name	Monensin
Contig	AF440781
Start / Stop / Direction	102,581 / 101,490 / - [in whole cluster] 102,581 / 101,490 / - [in contig]
Location	complement(101490..102581) [in whole cluster] complement(101490..102581) [in contig]
Type	CDS
Length	1,092 bp (363 aa)

Annotation

Category	4.3 transport
Product	putative small-conductance mechanosensitive channel
Product (GenBank)	unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	SCN_33
Reference	ACC Q846V8 PmId [12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003) comment monensin生合成 gene clusterの解析論文。 ORF33: Unknown 論文には詳細記載なし。 Fig. 2だとORF32に相当。
Related Reference	ACC P0C0S1 PmId [12015316] Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis. (J Biol Chem. , 2002) [10202137] Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. (EMBO J. , 1999) [12446901] Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. (Science. , 2002) [12080120] Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. (Biophys J. , 2002) comment 189th(P0C0S1) 29%, 9e-23 Escherichia coli (strain K12)_mscS Small-conductance mechanosensitive channel 大腸菌のSmall-conductance mechanosensitive channelの精製、リポソームでの構造、電動、開閉機構について。その他にも結晶構造解析の論文も含め多数、関連論文あり。

Sequence

>putative small-conductance mechanosensitive channel [unknown]
MTRALTLHDWIVAGIAVVAGVVAGLLLRALLRWLGERASKTRWSGDDVIVDALRTLVPCA
AITAGLAAAAGALPLTPRTGRNVTMTLTALLILAATLTAARIVTGLVKAVAQSRSGVAGS
ATIFVNITRVVVLAMGFLIVLQTLGISIAPLLTALGVGGLAVALALQDTLANLFAGVHIL
AAKTVQPGDYIQLSSGEEGYVVDINWRNTTVRQLSNNLVIIPNAKLAGTNMTNYSRPEQE
LSIMVQVGVSYDSDLEQVEKVTTEVVDEVMAEITGAVPDHEAAIRFHTFGDSRISFTVIL
GVGEFSDQYRIKHEFIKRLHQRYRAEGIRVPAPVRTVRVQQGELPPPLGIPHQRDTSTQA
RLH

>putative small-conductance mechanosensitive channel [unknown]
ATGACCCGTGCCCTCACACTGCACGACTGGATCGTGGCCGGTATCGCGGTCGTCGCCGGC
GTCGTCGCCGGGCTGCTGCTCCGCGCACTTCTGAGGTGGCTCGGCGAACGGGCGAGCAAA
ACCCGGTGGAGCGGTGACGACGTCATCGTCGACGCCCTGCGCACCCTCGTGCCCTGCGCG
GCCATCACCGCCGGGCTCGCCGCCGCGGCGGGCGCGCTGCCGCTCACGCCGCGCACCGGA
CGCAACGTCACCATGACGCTGACCGCGCTGCTCATCCTGGCCGCCACGCTCACGGCGGCC
CGGATCGTCACCGGCCTGGTGAAGGCCGTGGCACAGTCCCGCTCCGGAGTGGCCGGATCG
GCCACCATCTTCGTGAACATCACCCGCGTCGTGGTGCTCGCGATGGGCTTCCTCATCGTC
CTTCAGACCCTCGGCATCTCCATCGCACCGCTGCTCACCGCCCTCGGCGTGGGAGGTCTC
GCGGTCGCCCTCGCACTGCAGGACACGCTCGCCAATCTCTTCGCGGGCGTGCACATCCTC
GCCGCGAAGACCGTGCAGCCCGGCGACTACATCCAGCTCAGCAGCGGGGAGGAGGGCTAC
GTCGTCGACATCAACTGGCGCAACACCACGGTCCGCCAGCTCTCCAACAACCTCGTCATC
ATCCCCAACGCGAAGCTCGCCGGCACCAACATGACCAACTACAGCCGCCCCGAGCAGGAG
CTGTCGATCATGGTGCAGGTCGGGGTCAGCTACGACAGCGACCTGGAGCAGGTCGAGAAG
GTCACGACCGAGGTGGTGGACGAGGTGATGGCCGAGATCACCGGCGCGGTCCCCGACCAC
GAGGCGGCGATCCGCTTCCACACCTTCGGCGACTCGCGGATCAGCTTCACGGTGATCCTC
GGCGTCGGCGAGTTCAGCGACCAGTACCGCATCAAGCACGAGTTCATCAAGCGCCTGCAC
CAGCGTTACCGCGCCGAGGGCATCCGGGTCCCCGCCCCCGTGCGCACGGTCAGGGTGCAG
CAGGGCGAGCTGCCGCCGCCGCTCGGCATCCCGCACCAGCGCGACACCTCCACCCAGGCC
CGGCTGCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006685 Mechanosensitive ion channel MscS (Family) PF00924 MS_channel IPR010920 Like-Sm (LSM) domain (Domain) SSF50182 Sm_like_riboprot IPR011014 Mechanosensitive ion channel MscS, transmembrane-2 (Domain) SSF82861 MscS_transmembr IPR011066 Mechanosensitive ion channel MscS, C-terminal (Domain) SSF82689 MscC_channel_C
SignalP	Eukaryota
TMHMM	Transmembrane 5

Monen_00320 Monen_00340