Myxa_00010 : CDS information

Location

Organism	Stigmatella aurantiaca
Strain	Sga15
Entry name	Myxalamid
Contig	AF319998
Start / Stop / Direction	1,306 / 2 / - [in whole cluster] 1,306 / 2 / - [in contig]
Location	complement(2..1306) [in whole cluster] complement(2..1306) [in contig]
Type	CDS
Length	1,305 bp (434 aa)

Annotation

Category	5.1 general function
Product	putative aspartokinase putative aspartate kinase
Product (GenBank)	putative aspartokinase
Gene
Gene (GenBank)
EC number	2.7.2.4
Keyword
Note
Note (GenBank)	ORF1
Reference	ACC Q93TX4 PmId [11182319] Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15. (Chem Biol. , 2001) comment myxalamid生合成gene clusterの配列解析報告。 ORF1: aspartokinase Neisseria meningitidis(AE002499)へのid55%からaspartokinaseとしている。
Related Reference	ACC Q76E53 PmId [15277749] Characterization of the L-lysine biosynthetic pathway in the obligate methylotroph Methylophilus methylotrophus. (Biosci Biotechnol Biochem. , 2004) comment 117th(Q76E53) 58%, 1e-126 Methylophilus methylotrophus_ask Aspartokinase(EC 2.7.2.4) Methylophilus methylotrophusのask遺伝子を大腸菌で発現させ酵素活性を確認している。

Sequence

>putative aspartokinase [putative aspartokinase]
MRPIGSARQAGRGTSALALIVQKYGGTSVGDTERIKNVAKRCIAAQKAGHDVVVVVSAMS
GETNRLLKLVSQITERPNEREQDVVVATGEQVSIGLVALAIQTQKRKAVSFLGHQVRITT
DSTFSKARIKSIDAERIVDALKKKNIVVVAGFQGQDEHGNVTTLGRGGSDTTAVALAAAL
KADACEIYTDVDGVYTTDPNMVPAARKLERIAYEEMLELASVGAKVLQIRSVEFAMKYKV
PLWVKSSFTDDPGTLVCEEDAAMEDVLVSGIAYDKNEAKIAIRGVPDVPGVAAKIFGALD
EQSIVVDLIVQNVSKDGRTDVTFTVGKADLTKAKDVVKKIAKSVKAEGVETDDQVSKVSI
VGVGMRNHSGVAAKMFTVLAGAGVNVQMISTSEIKVSCVIHSNYTELAVRSLHTAFGLDK
PEAGAVSEVVSLKG

>putative aspartokinase [putative aspartokinase]
ATGCGCCCAATAGGGTCCGCGCGTCAAGCAGGAAGGGGCACAAGCGCCTTGGCACTCATC
GTCCAGAAGTATGGTGGCACCTCGGTCGGCGACACCGAGCGCATCAAGAACGTGGCGAAG
CGTTGCATCGCGGCCCAGAAGGCCGGGCATGACGTGGTGGTGGTGGTCTCCGCGATGTCC
GGAGAGACCAACCGGCTGCTTAAGCTCGTGTCCCAGATCACCGAGCGGCCCAACGAGCGC
GAGCAGGATGTGGTGGTGGCCACCGGGGAGCAGGTCTCCATCGGGCTCGTGGCCCTGGCC
ATCCAGACGCAGAAGCGCAAGGCGGTGAGCTTCCTGGGACACCAGGTCCGCATCACCACC
GACAGCACCTTCTCCAAGGCCCGTATCAAGAGCATCGACGCGGAACGCATCGTGGATGCG
CTGAAGAAGAAGAACATCGTCGTCGTGGCGGGCTTCCAGGGCCAGGACGAGCATGGCAAC
GTCACCACGTTGGGTCGCGGCGGATCGGACACCACGGCGGTGGCCCTGGCTGCCGCGCTC
AAGGCGGATGCCTGCGAGATCTACACGGATGTGGACGGTGTCTACACCACCGATCCCAAC
ATGGTCCCCGCGGCGCGCAAGCTCGAGCGCATCGCCTACGAGGAGATGTTGGAGCTGGCG
AGCGTGGGCGCCAAGGTGCTGCAGATCCGTTCGGTTGAGTTCGCGATGAAGTACAAGGTG
CCGCTCTGGGTGAAGTCCTCGTTCACGGACGATCCCGGCACGCTGGTGTGTGAGGAGGAC
GCTGCGATGGAAGACGTGCTGGTCAGTGGAATCGCCTACGACAAGAATGAGGCGAAGATC
GCCATCCGGGGCGTGCCGGACGTTCCCGGGGTGGCGGCGAAGATCTTCGGCGCGCTCGAT
GAGCAGAGCATCGTCGTGGATCTCATCGTCCAGAACGTCTCCAAGGATGGTCGCACGGAT
GTGACCTTCACCGTGGGCAAGGCGGACCTCACCAAGGCCAAGGACGTGGTGAAGAAGATC
GCCAAGTCCGTCAAGGCCGAGGGGGTAGAGACCGATGACCAGGTTTCCAAGGTGTCCATC
GTCGGCGTGGGCATGCGCAACCACTCGGGTGTGGCGGCGAAAATGTTCACGGTGCTCGCG
GGCGCGGGCGTCAACGTGCAGATGATCTCCACCTCGGAAATCAAAGTGTCCTGCGTCATC
CACTCCAACTACACGGAGCTGGCGGTCCGCTCGCTGCACACCGCGTTTGGCCTGGACAAG
CCCGAGGCGGGGGCTGTCTCCGAGGTTGTCTCCCTCAAGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001048 Aspartate/glutamate/uridylate kinase (Domain) PF00696 AA_kinase SSF53633 Aa_kinase G3DSA:3.40.1160.10 Aa_kinase IPR001057 Glutamate/acetylglutamate kinase (Family) PR00474 GLU5KINASE IPR001341 Aspartate kinase domain (Domain) TIGR00657 Asp_kinases IPR002912 ACT domain (Domain) PF01842 ACT IPR005260 Aspartate kinase, monofunctional class (Family) PIRSF000726 Asp_kin TIGR00656 Asp_kin_monofn IPR018042 Aspartate kinase, conserved site (Conserved_site) PS00324 ASPARTOKINASE
SignalP	No significant hit
TMHMM	No significant hit

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