Myxa_00020 : CDS information

Location

Organism	Stigmatella aurantiaca
Strain	Sga15
Entry name	Myxalamid
Contig	AF319998
Start / Stop / Direction	1,363 / 2,895 / + [in whole cluster] 1,363 / 2,895 / + [in contig]
Location	1363..2895 [in whole cluster] 1363..2895 [in contig]
Type	CDS
Length	1,533 bp (510 aa)

Annotation

Category	5.1 general function
Product	putative histidine ammonia-lyase putative histidase
Product (GenBank)	putative histidine ammonium lyase
Gene	hutH
Gene (GenBank)
EC number	4.3.1.3
Keyword
Note
Note (GenBank)	ORF2
Reference	ACC Q93TX3 PmId [11182319] Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15. (Chem Biol. , 2001) comment myxalamid生合成gene clusterの配列解析報告。 ORF2: histidine ammonia-lyase ORF2のmutantを作成することができなかった。これはORF2をコードする遺伝子が成長のために不可欠で、myxalamid生合成に関与していないことを示す。
Related Reference	ACC P10944 PmId [2454913] ( , ) comment Blast 93rd, id46%, 1e-125 Bacillus subtilis_hutH Histidine ammonia-lyase(EC 4.3.1.3) クローニングと活性の確認が行われている。

Sequence

>putative histidine ammonia-lyase [putative histidine ammonium lyase]
MSRPRLNIDGDTLKLEEILQVARHTVTVELAPAAAARVKAARDLVDRVAAGDTPSYGINT
GFGTLAEVRIDKKDLRELQRNLILSHAAGVGSPLPLPEARVLLLLRCNVLAKGYSGIRPE
TLALALEMLNRDVVPVVPERGSVGASGDLAPLAHLALVFIGEGEAFYKGERLPAAQALER
AGLKPVVLEAKEGLALVNGTQAMCAVGTLLQLRAEMLADLADLAGAMTLEGLLGSHKPFI
PEIQDVRAHEGQKACAAHLRELLADSALVESHVNCSKVQDPYSLRCMPQVHGAAREGLSF
ARRILEVEINSATDNPLVFVETERIVSGGNFHGQPVSLALDVAAMALTQLSAISERRVEQ
LVNPALSGLPPFLAKNSGLNSGFMIAQVTSAALVAESRVLSHPASVDSIPSSAGREDHVS
MGMTAALKGRQVADFTRSCLAIELLVAAQALDYRQPTRAGKGPQAAYELIRSKIPTMEKD
RELHRDIAAVSALIDSGELLNAVRTATRGQ

>putative histidine ammonia-lyase [putative histidine ammonium lyase]
ATGTCCCGCCCCCGCTTGAACATTGATGGAGACACTTTGAAGCTGGAGGAAATCCTCCAG
GTGGCCCGCCACACCGTCACCGTGGAGTTGGCCCCCGCGGCCGCTGCCCGCGTGAAGGCC
GCCCGGGATCTGGTGGACCGCGTGGCCGCCGGGGACACCCCCTCCTATGGCATCAACACC
GGCTTCGGAACGCTGGCCGAGGTCCGCATCGACAAGAAGGACCTGCGCGAGTTGCAGCGC
AACCTCATCCTCTCGCACGCCGCCGGGGTAGGCTCGCCGCTTCCGCTGCCCGAGGCCCGG
GTGCTGCTCTTGCTGCGTTGCAACGTGCTGGCCAAGGGCTATTCGGGCATCCGTCCGGAG
ACGCTGGCGCTCGCGCTGGAGATGCTCAACCGCGACGTGGTGCCCGTGGTGCCCGAGCGC
GGCAGCGTGGGGGCCTCGGGGGACCTGGCACCGCTGGCGCATCTGGCGCTGGTCTTCATC
GGCGAGGGCGAGGCGTTCTACAAAGGAGAGCGGCTCCCGGCGGCCCAGGCGCTGGAGCGC
GCCGGGCTGAAGCCGGTGGTGCTGGAGGCCAAGGAGGGGCTGGCGCTCGTCAACGGCACC
CAGGCCATGTGCGCGGTGGGCACCCTGCTCCAACTGCGCGCCGAGATGCTGGCGGACTTG
GCGGACCTGGCCGGGGCGATGACCCTGGAGGGGCTGCTGGGCAGCCACAAGCCCTTCATT
CCGGAGATCCAGGACGTGCGCGCCCACGAAGGCCAGAAGGCCTGCGCCGCCCACTTGCGC
GAGTTGCTGGCGGACAGCGCACTGGTGGAGAGCCACGTGAACTGCAGCAAGGTCCAGGAC
CCCTACAGCCTGCGCTGCATGCCCCAAGTGCACGGCGCGGCGCGCGAGGGGCTGTCCTTT
GCGCGGCGCATCCTGGAAGTGGAAATCAACAGCGCTACGGACAACCCCCTGGTGTTCGTG
GAGACGGAGCGCATCGTCTCGGGCGGCAACTTCCATGGCCAGCCGGTGTCCCTGGCGCTG
GACGTGGCGGCCATGGCGCTCACGCAACTGTCGGCCATCAGCGAGCGCCGCGTGGAGCAA
CTCGTCAACCCAGCGCTGTCGGGGCTGCCGCCGTTCCTTGCGAAGAATTCGGGGCTCAAC
TCGGGCTTCATGATTGCCCAGGTGACGAGCGCCGCGCTGGTGGCCGAATCCCGCGTGCTC
AGCCACCCCGCCTCGGTGGACTCCATCCCCTCCTCCGCAGGGCGAGAGGACCACGTCTCC
ATGGGCATGACAGCGGCGCTCAAGGGCCGTCAGGTGGCCGACTTCACCCGCTCGTGCCTC
GCCATCGAACTGCTGGTGGCGGCCCAGGCACTGGACTACCGCCAGCCCACCCGGGCGGGA
AAAGGCCCGCAGGCGGCGTACGAGCTCATCCGGAGCAAGATTCCCACCATGGAAAAGGAC
CGGGAGCTGCACCGGGATATCGCAGCCGTGAGCGCGCTCATCGACTCAGGTGAGCTGCTG
AACGCTGTGCGCACCGCGACGCGCGGACAGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001106 Aromatic amino acid lyase (Family) PF00221 Lyase_aromatic IPR005921 Histidine ammonia-lyase (Family) TIGR01225 HutH IPR008948 L-Aspartase-like (Domain) SSF48557 L-Aspartase-like IPR022313 Phenylalanine/histidine ammonia-lyases, active site (Active_site) PS00488 PAL_HISTIDASE IPR024083 L-Aspartase-like, N-terminal (Domain) G3DSA:1.10.275.10 G3DSA:1.10.275.10
SignalP	No significant hit
TMHMM	No significant hit

Myxa_00010 Myxa_00030