Myxa_00070 Myxa_00070   Myxa_00090 Myxa_00090

Myxa_00080 : CDS information

close this sectionLocation

Organism
StrainSga15
Entry nameMyxalamid
Contig
Start / Stop / Direction41,067 / 35,479 / - [in whole cluster]
41,067 / 35,479 / - [in contig]
Locationcomplement(35479..41067) [in whole cluster]
complement(35479..41067) [in contig]
TypeCDS
Length5,589 bp (1,862 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)MxaE
Gene
Gene (GenBank)mxaE
EC number
Keyword
Note
Note (GenBank)
  • PKS; contains KS, AT, DH, KR, and ACP regions
Reference
ACC
PmId
[11182319] Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15. (Chem Biol. , 2001)
comment
myxalamid生合成gene clusterの報告。
MxaE: PKS domains (KS, AT, DH, KR, ACP)

mxaEはmodule 2をコード。
DH domainは典型的なcore regionを持つが、産物の組み立てには不要なのでinactiveであると想定される。
mxaCでコードされるmodule 5は必要なDH domainを欠いており、このためにMxaEのDH domainが使用されるかもしれない。

close this sectionPKS/NRPS Module

2 methylmalonyl-CoA
not conserved YASHS(Y->V)
KS36..411
AT562..868
dh923..1092
KR1453..1639
ACP1737..1807

close this sectionSequence

selected fasta
>polyketide synthase [MxaE]
MTSFAEKIAQYSPKRLALLAMDLKSRLDAVEGTRSEPVAIIGMGCRFPGGGNDPESYWNL
LCNGVDAVTEVPSSRWTREEMERMDPEALEKLGARWGAFINKVDQFDADFFGISPREAHR
MDPQQRILLEVAWEALERAGQDMTQLAGSRTGVFVGIYSDDYALLQVGNPSARDSSSVTG
ALNCVVPGRLSYLLDFQGPCLAVDTACSSSLVALHLATQSLRNQECSMALACGVNLILSP
LSSSRVSRAQALAPDGRCKTFDARANGFVRGEGCGVVVLKRLSDAIAAGDPILALVRGSA
VNQDGKSAGLTAPNVLAQQALIRQALQSANLEPSEIDCVEAHGTGTSLGDPIEMEALHEV
YGSGRSAKQALVVGAAKTNIGHLESAAGIAGIIKMVLSMQHGAVPPIAHFQRLNPRIDFA
GESITIPTALHPWPAREERKRRGAISSFGISGTNAHVILEEAPAPQTASASRTTGAHLLP
LSARSPSALQELAQKYAEYLVSSPEASLREVCYTAAQRRTHHEYRLAVAGESAAAVAEKL
RELASGAPVPAVNGLDERRKVVFVFPGQGSQWLGMGRQLIDQEPVFRETLERIDEAMRPH
VTWRLIDVLRAPSESSRLQEIDVVQPVLFAMAVALSALWRSWGIEPDAVVGHSMGEVAAA
HVAGALSLEDAAAVICLRSRLLKRISGQGAMLATELSLEDARKALAGREDRVAIAVSNSP
TSTVLSGDAAALEEIRVSLEARNVFCRWVKVDVASHSPQVDPLRADLLSVLSAVRPRPST
VPIVSTVTAATCDGSGFDASYWVRNLRDPVLFSSAVLDLVKGGHTVFMEMSPHPILLPAV
ERCMQHAGREGVTIPSLRREEAERTVMLESVGALYRTAYPVEWKRLFPEGGRMAPLPTYP
WQHKRFWLDVVAAPVLSAAEQVTSLKGRPVSVAHGVDGEIFELELSGTSLPWLSAHRLGG
VAVVPASALVELGLSAAAEALGAGARELSDVEFERALVLTEADRRLVQVHLSPASGGQHV
FHIHSRAVGGTSPGAGWVRHCRGQIRAANTPSGSPEVLDAVRSRCAQHVQGAAVYEQLER
CNVQYEAPLRTLGEAWRRPGEALGAVALSPEVIQESARYQLHPALLDAGLQTLALALAAE
SGEAVLFMPLSIGSLECVQGRADVKWAHVSIAAATSPEDRMGTLELLDGEGRRVAVARGV
RLRRVAAERLLEVLGEARSQEWFHDVTWEPRPAVTAQPGPADWLVFLDRGGWGTALVEEI
GRQGQPCVTVTAGETFQRQDARRFVVNPKRPEDMERLLRELPALPAGHEGRAVYLWGLDA
VLDEQTGTPESSVAALHLVKALMGSPARARLWVVTRGAQVTGVGTERVSLAQAPLWGMGR
SVSLEQPGVWGGLIDLAPERVAEETAAVLREISAFGGDGEDQIALREKRSLVPRIARGRV
NAPAEPLRLRPDGAYLVTGGLGGLGLKVARWLVARGARHLVLLGRSGASGAGDAASARRR
EGIESLRALGASVTTLAVDVADREKLAALLREAAATLPPLRGVIHAAALLTESNLENMDL
AAMTAMMRPKVLGSWVLHEVTREAELDFFVMFSSTSTLWGASGLAHYAAGNQFLEALAHH
RRAQGLPATTIHWGTWDEIGGERADNERGYARFGLNPMSSEPALEAMGQVLRAGVSHKTI
ASVNWSVLKPIWEARRRRPFLQHMGESLPTPGATASSRARLLTELQALPPARRFDTLVQR
IQSEVGRILGFAASDLPSTERGFFQMGMTSQMSVELRNALQRGLEKELPASLAFDHPTVI
ALAKRLASSVTAVEIPLPTVAVAQESRPQAAQAEIGDLAERLSQVTELSDEEVERLIAQK
LS
selected fasta
>polyketide synthase [MxaE]
ATGACGAGTTTCGCAGAGAAGATCGCGCAGTACTCCCCCAAGCGGCTTGCGCTCCTCGCA
ATGGATCTCAAGTCCCGGCTCGATGCGGTGGAGGGCACACGATCGGAGCCGGTAGCGATC
ATCGGAATGGGGTGTCGCTTCCCCGGGGGGGGGAACGATCCGGAGTCGTACTGGAACCTT
CTGTGCAACGGCGTGGATGCTGTCACCGAAGTGCCCTCCTCGCGGTGGACCCGGGAGGAG
ATGGAGCGGATGGACCCCGAGGCCCTGGAGAAGCTGGGGGCGCGGTGGGGGGCCTTCATC
AACAAGGTGGACCAGTTCGACGCGGATTTTTTCGGGATCTCGCCACGCGAGGCGCACCGG
ATGGATCCGCAGCAGCGCATCCTGCTTGAGGTCGCGTGGGAGGCGCTGGAGCGCGCTGGG
CAGGACATGACGCAGCTGGCGGGCAGCCGCACCGGCGTGTTCGTGGGGATTTACAGCGAC
GACTACGCGTTGCTGCAGGTGGGTAATCCCTCCGCTCGGGATTCGAGCAGCGTGACGGGG
GCGCTCAACTGCGTGGTGCCGGGGCGCTTGTCCTATCTCCTGGATTTCCAGGGGCCCTGC
CTGGCCGTGGACACCGCGTGCTCGTCGTCCCTCGTAGCGCTGCATCTGGCCACCCAGAGC
CTCCGCAACCAGGAGTGCTCGATGGCGCTGGCATGCGGCGTCAACCTCATCCTCTCTCCG
CTCTCGTCGAGCCGCGTTTCGCGGGCTCAGGCGCTCGCGCCGGATGGCCGGTGCAAGACC
TTCGATGCGCGCGCCAATGGTTTCGTGCGAGGAGAGGGCTGCGGCGTCGTCGTCCTCAAG
CGGTTGTCCGATGCGATCGCGGCGGGGGACCCCATCCTGGCGCTCGTGCGGGGCTCGGCC
GTCAATCAGGATGGCAAGTCGGCGGGGCTGACCGCGCCCAACGTGCTTGCCCAGCAGGCG
CTCATCCGCCAGGCGCTGCAGAGCGCGAACCTCGAGCCCTCCGAGATCGACTGTGTCGAG
GCGCACGGGACGGGAACCTCGCTGGGGGATCCCATTGAGATGGAGGCGCTCCATGAGGTC
TACGGGAGCGGACGCTCCGCGAAGCAAGCCCTCGTCGTGGGTGCGGCGAAGACCAACATT
GGCCACTTGGAGTCCGCCGCGGGAATCGCGGGCATCATCAAGATGGTCCTCTCCATGCAG
CACGGGGCCGTCCCACCGATCGCGCATTTCCAGCGGCTCAACCCACGGATCGACTTCGCC
GGTGAGTCCATCACCATCCCCACGGCGCTCCATCCCTGGCCTGCCCGGGAAGAGCGGAAG
CGCCGAGGGGCGATCAGCTCGTTCGGCATCAGTGGGACGAACGCGCATGTGATTCTCGAG
GAGGCGCCCGCGCCTCAGACGGCGTCGGCTTCGCGCACCACGGGGGCGCACCTGCTGCCG
TTGTCGGCACGCTCGCCCTCCGCGCTCCAGGAGTTGGCCCAGAAGTATGCGGAGTACCTC
GTGTCGTCGCCGGAGGCGTCCCTCCGGGAGGTTTGCTACACGGCGGCGCAGCGGCGGACA
CACCATGAGTACCGCTTGGCCGTCGCGGGCGAGTCCGCGGCCGCCGTCGCCGAGAAGCTC
CGGGAGCTTGCCTCGGGAGCCCCTGTTCCGGCTGTGAATGGCTTGGACGAGCGGCGCAAG
GTGGTCTTCGTGTTTCCCGGCCAGGGCTCCCAGTGGCTCGGCATGGGGCGGCAGCTCATC
GATCAGGAGCCCGTCTTCCGCGAGACTCTCGAGCGCATCGACGAGGCCATGCGGCCTCAC
GTCACGTGGCGGCTGATCGACGTGCTCCGTGCGCCGTCCGAGTCGTCTCGGCTCCAAGAG
ATCGACGTCGTGCAGCCGGTGCTGTTCGCCATGGCCGTGGCGCTCTCGGCACTCTGGCGC
TCGTGGGGCATCGAGCCGGACGCGGTGGTCGGGCACAGCATGGGCGAGGTGGCTGCGGCA
CACGTGGCGGGCGCCCTCAGCCTGGAGGATGCGGCGGCCGTCATTTGCCTCCGGAGTCGG
CTGCTCAAGCGCATCAGCGGACAGGGCGCGATGTTGGCCACGGAGTTGTCCTTGGAGGAC
GCTCGGAAGGCCCTGGCGGGGCGCGAAGATCGCGTCGCGATTGCGGTGAGCAACAGCCCG
ACCTCGACGGTGTTGTCCGGGGATGCCGCGGCACTCGAGGAGATCCGCGTCTCGCTCGAG
GCGCGCAATGTGTTCTGCCGCTGGGTGAAGGTCGACGTGGCCTCGCACAGCCCGCAGGTC
GATCCTTTGCGTGCGGATCTGCTCTCCGTGCTCTCGGCGGTGCGGCCCCGGCCTTCCACC
GTGCCCATCGTTTCGACCGTGACCGCCGCTACCTGCGACGGGTCGGGCTTCGATGCTTCG
TACTGGGTTCGCAACCTGCGCGATCCGGTGCTGTTCTCCTCGGCCGTGCTCGATCTGGTC
AAGGGGGGGCACACCGTCTTCATGGAGATGAGCCCGCATCCAATCCTGTTGCCCGCCGTC
GAGCGGTGCATGCAGCACGCGGGTCGCGAGGGGGTGACGATTCCCTCTCTGCGCCGTGAG
GAGGCCGAACGGACCGTGATGCTGGAGTCCGTTGGCGCGCTGTACCGCACGGCTTACCCC
GTGGAGTGGAAGCGGCTCTTCCCGGAAGGCGGGCGGATGGCGCCGCTGCCGACGTATCCC
TGGCAGCACAAGCGGTTCTGGCTCGATGTGGTGGCAGCGCCCGTTCTTTCCGCCGCCGAG
CAGGTGACGTCCCTGAAGGGGCGGCCGGTGAGCGTCGCGCACGGGGTCGATGGCGAGATC
TTCGAACTGGAGTTGAGCGGTACCTCTCTGCCGTGGCTCAGTGCGCACCGGTTGGGCGGC
GTCGCCGTGGTGCCGGCTTCGGCCTTGGTCGAACTGGGGTTGAGCGCCGCGGCCGAGGCG
CTGGGAGCAGGAGCTCGGGAGCTCTCGGACGTGGAGTTCGAGCGCGCGTTGGTGTTGACG
GAGGCAGATCGGCGCCTCGTGCAAGTACACCTCTCTCCGGCTTCCGGTGGGCAGCACGTG
TTCCACATCCACAGCCGTGCGGTGGGTGGCACGTCGCCCGGAGCGGGGTGGGTACGGCAT
TGCCGCGGTCAGATCCGGGCGGCGAACACGCCCTCGGGATCCCCAGAGGTCCTTGATGCG
GTGCGCTCGCGCTGCGCGCAGCACGTACAAGGGGCGGCTGTTTACGAGCAACTCGAACGC
TGCAACGTTCAGTACGAGGCTCCGTTGCGAACGCTCGGCGAAGCCTGGAGGCGGCCAGGC
GAGGCGCTCGGGGCCGTTGCGCTGAGCCCAGAAGTGATTCAGGAGTCGGCGCGCTATCAA
CTCCACCCGGCCCTTCTGGACGCGGGGTTGCAGACCTTGGCGCTCGCGCTCGCGGCGGAG
TCGGGTGAGGCCGTGTTGTTCATGCCCTTGAGCATCGGGTCGCTCGAGTGCGTTCAGGGC
CGTGCCGACGTCAAGTGGGCCCACGTGTCCATTGCTGCCGCCACCAGCCCGGAAGACCGG
ATGGGCACGCTGGAGTTGCTGGATGGAGAGGGGCGCCGGGTGGCTGTGGCGCGCGGTGTG
CGGTTGCGGCGCGTGGCGGCCGAGCGTCTCCTCGAGGTGCTGGGCGAAGCGCGTTCGCAA
GAGTGGTTCCATGACGTGACGTGGGAGCCCAGGCCTGCTGTCACGGCCCAGCCAGGGCCT
GCGGATTGGCTCGTGTTCCTCGATCGGGGCGGGTGGGGCACCGCGCTTGTGGAAGAGATT
GGCCGTCAGGGCCAACCGTGCGTCACGGTGACGGCGGGGGAGACCTTCCAGCGGCAGGAC
GCGCGGCGCTTCGTGGTGAATCCGAAGCGGCCGGAGGACATGGAGCGTCTGCTGCGCGAG
TTGCCAGCACTGCCCGCCGGGCATGAAGGCCGCGCCGTCTACCTGTGGGGCCTCGATGCG
GTGCTCGACGAGCAGACGGGCACGCCGGAGTCCTCCGTGGCCGCGCTGCATCTGGTGAAG
GCCCTCATGGGCTCACCGGCGCGCGCGCGGCTCTGGGTGGTGACGCGGGGCGCTCAAGTC
ACTGGCGTGGGGACCGAGCGCGTGTCGCTGGCTCAGGCGCCGCTCTGGGGCATGGGTCGC
AGCGTGTCCCTCGAGCAGCCAGGGGTGTGGGGTGGCCTGATTGATCTGGCCCCGGAGAGA
GTCGCCGAGGAGACCGCTGCGGTGCTTCGCGAGATCTCCGCGTTTGGCGGGGATGGTGAG
GATCAGATCGCGCTTCGCGAGAAGCGTTCACTGGTGCCTCGCATCGCTCGCGGGCGGGTA
AATGCGCCAGCGGAGCCGCTGCGCCTGCGTCCGGATGGGGCCTACCTCGTCACGGGCGGG
CTGGGCGGGCTGGGCCTGAAGGTGGCGCGGTGGCTGGTGGCGCGGGGTGCGCGGCACCTC
GTGCTGCTGGGCCGGAGTGGAGCCTCCGGCGCAGGGGATGCGGCTTCGGCGCGGCGCCGC
GAGGGGATCGAATCGCTCCGGGCGCTCGGCGCGTCCGTCACCACGCTGGCCGTCGACGTG
GCGGATCGCGAGAAGCTGGCGGCGCTCCTGCGCGAGGCGGCTGCGACGCTTCCTCCGTTG
CGAGGGGTGATTCATGCCGCAGCGCTTCTGACCGAGAGCAACTTGGAGAACATGGATCTC
GCGGCGATGACCGCGATGATGCGCCCCAAGGTGCTTGGCAGTTGGGTGCTTCACGAGGTG
ACGCGCGAGGCCGAGCTCGACTTCTTCGTGATGTTCTCGTCGACGTCGACCCTGTGGGGT
GCCTCGGGGTTGGCGCACTACGCAGCGGGCAACCAGTTCCTCGAGGCCCTGGCGCACCAT
CGCCGGGCCCAGGGGCTGCCTGCGACCACCATTCACTGGGGGACGTGGGACGAGATCGGC
GGGGAGCGTGCGGACAACGAGCGGGGTTACGCGCGTTTCGGGCTGAATCCCATGTCTTCG
GAGCCGGCGCTCGAGGCGATGGGGCAGGTGCTGCGGGCGGGAGTGAGTCACAAGACCATT
GCGTCCGTGAACTGGTCCGTGCTGAAGCCCATCTGGGAGGCACGGCGGCGGCGGCCCTTC
TTGCAGCACATGGGCGAGTCCTTGCCGACACCGGGGGCGACGGCGTCCTCGCGGGCGCGA
CTGCTCACGGAACTCCAGGCGCTTCCTCCTGCCCGTCGCTTCGACACGCTGGTGCAACGC
ATTCAGAGCGAGGTAGGACGGATCCTAGGATTCGCTGCTTCTGATCTGCCCTCCACGGAG
CGGGGGTTCTTCCAGATGGGAATGACTTCACAGATGTCCGTGGAACTGCGCAATGCGCTG
CAACGGGGGCTCGAGAAGGAGCTGCCCGCGAGCCTCGCGTTCGATCACCCCACCGTCATC
GCGTTGGCGAAGCGTCTGGCTTCGAGTGTCACCGCGGTGGAAATCCCACTGCCTACGGTG
GCAGTGGCTCAAGAGTCGCGGCCGCAGGCCGCGCAGGCGGAAATCGGTGATCTCGCCGAG
CGCCTGTCGCAGGTTACAGAACTGTCCGACGAAGAAGTCGAGCGGTTGATTGCACAAAAG
CTGAGCTGA
[2] KS36..411
[2] AT562..868
[2] methylmalonyl-CoA not conserved YASHS(Y->V)753..757
[2] dh923..1092
[2] KR1453..1639
[2] ACP1737..1807
[2] KS106..1233
[2] AT1684..2604
[2] methylmalonyl-CoA not conserved YASHS(Y->V)2257..2271
[2] dh2767..3276
[2] KR4357..4917
[2] ACP5209..5421

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[557-685] 4.59999999999996e-78 G3DSA:3.40.366.10 [753-870] 4.59999999999996e-78 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1453-1626] 1e-56 PF00106
PF00106   adh_short
IPR009081 Acyl carrier protein-like (Domain)
[1730-1844] 2.19999900980708e-14 SSF47336
SSF47336   ACP_like
[1737-1807] PS50075
PS50075   ACP_DOMAIN
[1739-1805] 1e-07 PF00550
PF00550   PP-binding
[1735-1809] 9.8e-12 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[36-285] 5.69999999999998e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[293-411] 1.70000000000001e-43 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[562-868] 1.60000000000002e-97 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[560-862] 2.2999842048857e-70 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[687-752] 5.99999044089058e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[36-295] 8.80000000000003e-100 G3DSA:3.40.47.10 [297-464] 9.29999999999998e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[28-464] 1.19999063421924e-100 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1454-1656] 1.99999999999999e-39 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[198-214] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[564-861] 5.60004908290974e-124 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1738-1810] 4.00000300869883e-21 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[923-1092] 6.10002656149838e-34 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[38-464] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1453-1639] 1.99999636034521e-58 SM00822
SM00822   PKS_KR
SignalP
[1-29] 0.317 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Myxa_00070 Myxa_00070   Myxa_00090 Myxa_00090
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