Myxa_00100 : CDS information

Location

Organism	Stigmatella aurantiaca
Strain	Sga15
Entry name	Myxalamid
Contig	AF319998
Start / Stop / Direction	48,435 / 49,295 / + [in whole cluster] 48,435 / 49,295 / + [in contig]
Location	48435..49295 [in whole cluster] 48435..49295 [in contig]
Type	CDS
Length	861 bp (286 aa)

Annotation

Category	5.1 general function
Product	putative hydrolase
Product (GenBank)	putative acetyl transferase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF3
Reference	ACC Q93TW5 PmId [11182319] Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15. (Chem Biol. , 2001) comment myxalamid生合成gene clusterの配列解析報告。 ORF3: acetyl-transferase ORF3はacetyl-transferasesに類似性あり。 mxaFと分かれて転写され、tandem promotorがmxaFとの間の264bp gap内にある。 myxalamid生合成における役割は不明。
Related Reference	ACC P00632 PmId [3003031] Cloning and expression of Acinetobacter calcoaceticus catBCDE genes in Pseudomonas putida and Escherichia coli. (J Bacteriol. , 1986) [1158871] Beta-ketoadipate enol-lactone hydrolases I and II from Acinetobacter calcoaceticus. (J Biol Chem. , 1975) [670169] Homologous amino acid sequences in enzymes mediating sequential metabolic reactions. (J Biol Chem. , 1978) comment 375th(P00632) 30%, 6e-08 Acinetobacter sp._catD 3-oxoadipate enol-lactonase 2(EC 3.1.1.24) PMID:9811637よりAcinetobacter calcoaceticus→Acinetobacter sp.に変更(Acinetobacter sp. strain ADP1 is synonymously called Acinetobacter sp. strain BD413 and was formerly classified as Acinetobacter calcoaceticus ADP1.) CATALYTIC ACTIVITY: 3-oxoadipate enol-lactone + H2O = 3-oxoadipate. PATHWAY: Catabolism of catechol to succinate and acetyl-CoA in the beta-ketoadipate pathway; fourth step.

Sequence

>putative hydrolase [putative acetyl transferase]
MRKWLVAFWIGATVALTGCSDEPGPLDDPPVLVDHWLTASDGMKLHYLELEGEGTPVVLL
HGFLGTASGTWVAPGFAQALAAAGHRVILLDQRGHGESDKPLEPSAYGEQMVTDVIEMLD
DLKINQAHVGGYSMGGEMTAMLMRRVPERFITAHLGGAGIVETDEALRAAAAALDPKGTD
PEEPRILALFEEATANLPPPDPRVIAAMTEAWAVWGPRPVDLTVIDFPVLTVNGEFDNPY
SKTLRMTRELSQFENVVVPRRSHLTTLIDPLYLQSLVAFIDTHDPR

>putative hydrolase [putative acetyl transferase]
ATGCGGAAGTGGCTCGTAGCGTTCTGGATAGGTGCAACCGTGGCACTCACCGGCTGTTCT
GACGAGCCGGGCCCTTTAGATGATCCACCCGTCCTTGTCGATCACTGGTTGACCGCCAGC
GATGGGATGAAGTTGCACTATCTGGAGCTTGAAGGAGAAGGCACACCCGTCGTGCTTCTG
CACGGATTCTTGGGAACAGCGAGCGGAACCTGGGTCGCTCCAGGCTTCGCACAAGCCCTG
GCGGCGGCAGGGCACCGGGTCATTCTCCTCGACCAACGCGGCCACGGTGAGAGTGACAAA
CCCCTCGAGCCTTCGGCCTACGGGGAGCAAATGGTCACCGATGTCATCGAAATGCTGGAC
GATTTGAAAATCAACCAAGCCCACGTGGGTGGCTACTCCATGGGCGGAGAAATGACAGCC
ATGCTGATGAGACGGGTGCCGGAGAGATTCATCACCGCCCATCTCGGGGGGGCGGGCATT
GTGGAAACCGACGAGGCGCTCAGGGCCGCGGCCGCAGCATTGGATCCAAAAGGGACGGAT
CCCGAAGAGCCTCGGATCCTGGCCCTCTTTGAAGAGGCCACGGCGAACCTGCCCCCACCG
GATCCCCGTGTGATCGCAGCCATGACCGAGGCCTGGGCGGTCTGGGGGCCCCGGCCCGTC
GATCTGACCGTCATCGACTTTCCCGTACTGACCGTGAACGGCGAATTCGACAATCCCTAC
TCGAAGACCCTGCGGATGACGCGCGAATTAAGCCAATTCGAGAATGTCGTCGTACCGCGC
CGCTCACATCTCACAACCCTCATCGATCCGCTCTATCTTCAGAGCCTCGTGGCATTCATC
GACACCCACGATCCCCGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Myxa_00090 Myxa_00110