Nanch_00030 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	3,117 / 3,818 / + [in whole cluster] 3,117 / 3,818 / + [in contig]
Location	3117..3818 [in whole cluster] 3117..3818 [in contig]
Type	CDS
Length	702 bp (233 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative two-component system response regulator
Product (GenBank)	NanT3
Gene
Gene (GenBank)	nanT3
EC number
Keyword
Note
Note (GenBank)	putative two-component response regulator
Reference	ACC Q7WTG0 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003) comment Nanchangmycin生合成のためのcomplete gene clusterの報告。（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）配列解析からの機能予測 NanT3: Two-component response regulator nanR1 and nanR2の下流に明らかなtwo-component signal transduction systemがある。これはNanT5: putative sensor histidine kinaseとNanT3: putative response regulator proteinから成る。両者はnitrate/nitrite pathway regulatorsのメンバーにとても似ている。
Related Reference	ACC Q9RIM0 PmId [16272382] Identification of a novel two-component system SenS/SenR modulating the production of the catalase-peroxidase CpeB and the haem-binding protein HbpS in Streptomyces reticuli. (Microbiology. , 2005) comment 35th(Q9RIM0) 35%, 2e-14 Streptomyces reticuli_senR Response regulatory protein SenSは、N末に5つの膜貫通domainsが予測されるsensor histidine kinaseである。 SenRは、C末 DNA-binding motifのあるresponse regulatorである。新規のtwo-component system SenS/SenRは、furS-cpeB operon と hbpS geneの発現をnegativeに調節する。SenS/SenRの存在は、S. reticuliにおけるhaemin と redox-cycling compound plumbaginへの抵抗性をかなり拡大する。
Related Reference	ACC Q9RPE9 PmId [10464204] Identification of a two-component signal transduction system from Corynebacterium diphtheriae that activates gene expression in response to the presence of heme and hemoglobin. (J Bacteriol. , 1999) comment 110th(Q9RPE9) 32%, 8e-11 Corynebacterium diphtheriae_chrA Response regulator ChrA ChrSはsensor kinaseで、Hemeを感知するとChrAへシグナルを送る。（Pリレー）シグナルを受け取ったrespons regulatorであるChrAがhmuOプロモーターの転写活性を上げることを確認している。

Sequence

>putative two-component system response regulator [NanT3]
MSTRCTGSLTNGWVSVELGIPLSVVLVDDHPVVRAGISAWCAAADAPISVVAEGANVSVA
LHGPGRGADVVVMDLLLQNGRPAYDELQELVAQERKVVVYTMRDSQDAALTCMDLGSATY
ITKAEGQRHLVKAIRAAAEDIPYTPPSLAGAFGSDTRQSRPVLSVREVEVLVEWFQSESK
AVVAQSLGISERTVNTYLDRVRIKYANAGRPATTKAKLVARAVQDGLIALDEL

>putative two-component system response regulator [NanT3]
TTGTCTACTCGTTGTACAGGTTCTCTCACGAATGGGTGGGTGAGTGTGGAGTTAGGAATT
CCCCTCAGCGTCGTGTTGGTCGATGATCACCCCGTGGTGCGCGCGGGCATCTCGGCGTGG
TGTGCCGCCGCCGACGCACCGATCAGCGTGGTCGCCGAGGGGGCGAACGTGTCGGTGGCC
CTCCATGGTCCGGGCCGGGGGGCGGATGTCGTGGTCATGGATCTGTTGTTGCAGAACGGG
CGGCCCGCGTACGACGAGTTGCAGGAGCTCGTGGCCCAGGAGCGCAAGGTCGTCGTCTAC
ACCATGCGGGACAGCCAGGATGCGGCGTTGACCTGCATGGATCTGGGCTCGGCTACCTAC
ATCACCAAGGCGGAGGGGCAGCGGCACCTGGTCAAGGCGATCCGGGCCGCCGCCGAGGAC
ATCCCCTATACACCCCCGTCCCTGGCCGGCGCGTTCGGCAGCGACACCCGGCAGAGCCGC
CCCGTGCTGTCCGTACGGGAGGTGGAGGTGCTGGTGGAGTGGTTCCAGTCCGAGTCGAAG
GCGGTGGTCGCCCAGAGCCTGGGGATCTCGGAGCGCACGGTGAACACCTATCTGGACCGG
GTGCGCATCAAGTACGCGAACGCCGGCCGGCCGGCGACCACGAAGGCCAAGCTGGTGGCC
AGGGCGGTTCAGGACGGTCTGATAGCTCTGGACGAGCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) SM00421 HTH_LUXR PF00196 GerE IPR001356 Homeodomain (Domain) SM00389 HOX IPR001789 Signal transduction response regulator, receiver domain (Domain) PS50110 RESPONSE_REGULATORY SM00448 REC PF00072 Response_reg IPR011006 CheY-like superfamily (Domain) SSF52172 CheY_like IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

Nanch_00020 Nanch_00040