Nanch_00050 Nanch_00050   Nanch_00070 Nanch_00070

Nanch_00060 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameNanchangmycin
Contig
Start / Stop / Direction7,493 / 6,768 / - [in whole cluster]
7,493 / 6,768 / - [in contig]
Locationcomplement(6768..7493) [in whole cluster]
complement(6768..7493) [in contig]
TypeCDS
Length726 bp (241 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)NanR1
Gene
Gene (GenBank)nanR1
EC number
Keyword
  • SARP family
Note
Note (GenBank)
  • putative regulatory protein
Reference
ACC
PmId
[12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
comment
Nanchangmycin生合成のためのcomplete gene clusterの報告。
（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）

配列解析からの機能予測
NanR1: Regulatory protein

nanR1 and nanR2はPKS loading moduleの上流にあり、Streptomyces antibiotic regulatory protein(SARP) familyに属するtranscriptional activatorsに似ている。

SARP familyのタンパクはN末に、E.coli activator OmpRのC末にあるDNA binding domainに似たAA motifsを持っている。このmotifsは、NanR1 and NanR2にもとてもはっきりと見られる。

転写を活性化するため、Streptomycesの抗生物質生合成遺伝子の上流にある特異的配列とSARPsは相互作用すると信じられている。特徴的なSARP binding sitesは、nanT3, nanT5, and nanR2の上流にみられる。
Related Reference
ACC
Q846W3
NITE
Monen_00280
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
comment
5th(Q846W3) 53%, 5e-58
Streptomyces cinnamonensis_monRI
activator protein

MonRI は、SARP familyのpathway-specific activatorsに相同性あり。
monRI をpromoter下に入れて過剰発現させるとmonensin産生が増えることを確認している。

close this sectionSequence

selected fasta
>putative transcriptional regulator [NanR1]
MGPLRVVDEKGGSPLRAQKLAAVLAVLLIRSDQVVMAEQLTREIWGELPPRRDTASIYVC
ISRLRKFLSRPGQQNPIITRPGGYMLRRASSECDFDVFQQLVADGRKCVRYGEPAQAALC
FEQALALWRGPAFGNVQTGPIVAGFLKRLDEMRIECAEMSAEAHLELGRHRELVGRLRSL
VAEFPLRETFYRQLMLALYRSDQKSEALGIYESARRVLDRELGLEPGRKLRELRHTVLAS
T
selected fasta
>putative transcriptional regulator [NanR1]
TTGGGACCTCTCCGTGTAGTCGACGAAAAGGGCGGTTCACCGCTCAGAGCGCAGAAACTC
GCTGCCGTTCTCGCCGTCCTGCTGATCCGCTCGGATCAGGTTGTGATGGCGGAACAGCTC
ACCAGAGAGATCTGGGGCGAGCTGCCGCCGCGCCGCGACACCGCGAGCATCTACGTCTGC
ATTTCGCGGCTTCGCAAGTTCCTGAGCCGGCCGGGCCAGCAGAATCCCATCATCACCCGC
CCCGGTGGATACATGCTGCGCAGAGCGTCCAGCGAATGCGATTTCGATGTCTTCCAGCAG
CTGGTCGCGGACGGCCGGAAGTGCGTCAGATACGGGGAGCCGGCGCAGGCGGCCCTCTGT
TTCGAGCAGGCCCTGGCGCTGTGGCGGGGCCCGGCGTTCGGCAACGTCCAGACCGGGCCG
ATCGTGGCCGGCTTTCTGAAGCGGCTGGACGAGATGCGCATCGAATGTGCGGAAATGTCG
GCCGAGGCGCATCTTGAACTCGGCCGCCACCGGGAACTCGTCGGCCGACTACGTTCCCTG
GTCGCCGAGTTCCCGCTCCGGGAGACGTTCTATCGGCAGCTGATGCTGGCCCTCTACCGC
TCCGACCAGAAGTCAGAGGCGCTTGGCATATACGAGTCCGCCCGGCGTGTTCTCGACAGG
GAACTGGGGCTCGAACCCGGCCGGAAGCTCAGGGAGCTCCGGCACACGGTGCTCGCCTCG
ACCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[12-86] 5.20000089003336e-14 SM00862
SM00862   Trans_reg_C
[18-86] 6.60000000000002e-13 PF00486
PF00486   Trans_reg_C
IPR005158 Bacterial transcriptional activator domain (Domain)
[93-238] 6.39998633403535e-52 SM01043
SM01043   BTAD
[93-238] 8.60000000000004e-51 PF03704
PF03704   BTAD
IPR011990 Tetratricopeptide-like helical (Domain)
[99-216] 8.3e-07 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[21-87] 4.6e-10 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[1-87] 2.00000075217456e-12 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP
[1-37] 0.967 Signal
Eukaryota   
[1-37] 0.336 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Nanch_00050 Nanch_00050   Nanch_00070 Nanch_00070
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