Nanch_00050 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	6,106 / 4,916 / - [in whole cluster] 6,106 / 4,916 / - [in contig]
Location	complement(4916..6106) [in whole cluster] complement(4916..6106) [in contig]
Type	CDS
Length	1,191 bp (396 aa)

Annotation

Category	5.1 general function
Product	putative two-component system histidine kinase
Product (GenBank)	NanT5
Gene
Gene (GenBank)	nanT5
EC number
Keyword
Note
Note (GenBank)	putative two-component sensor histidine kinase
Reference	ACC Q7WTF8 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003) comment Nanchangmycin生合成のためのcomplete gene clusterの報告。（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）配列解析からの機能予測 NanT5: Two-component sensor histidine kinase nanR1 and nanR2の下流に明らかなtwo-component signal transduction systemがある。これはNanT5: putative sensor histidine kinaseとNanT3: putative response regulator proteinから成る。両者はnitrate/nitrite pathway regulatorsのメンバーにとても似ている。
Related Reference	ACC Q9RIM1 PmId [16272382] Identification of a novel two-component system SenS/SenR modulating the production of the catalase-peroxidase CpeB and the haem-binding protein HbpS in Streptomyces reticuli. (Microbiology. , 2005) comment blast search id32.32%, 2e-05 Streptomyces reticuli_senS Histidine autokinase SenSは、N末に5つの膜貫通domainsが予測されるsensor histidine kinaseである。 SenRは、C末 DNA-binding motifのあるresponse regulatorである。新規のtwo-component system SenS/SenRは、furS-cpeB operon と hbpS geneの発現をnegativeに調節する。SenS/SenRの存在は、S. reticuliにおけるhaemin と redox-cycling compound plumbaginへの抵抗性をかなり拡大する。
Family/Domain	FD IPR003594 comment [IPR003594] ATPase-like, ATP-binding domain (Domain)

Sequence

>putative two-component system histidine kinase [NanT5]
MSISSLSPGIDPPASARLPGSAGPWAREYVAFNRRLALYGRAGVMSACAVLGVAAMPMGE
MAPAAVVAAAVLAWSWIHLRLATTSAMRQRPLLALDLTVMTALCLSQRFTIPEAQTTHAG
TWILVSVSFTAVAYQLMQPPLTGILATLWLCCADVVGTALHPNLEWSGVLRSVIWVVVNT
ALARAVVRLVFSESRAADEAADRAARARQQGEAAEARCAAEREHLAALHDTACATLLIAS
APWASMRAETLRAQAARDLLRLRAEQPVAGEVDLAAELLDEIAAHPLRVINRFDVELGTT
WRPIAAALRGGLGEALRNVARYAGVDAVRVTAERVDHVIVVTIADSGVGFDPEHIPGHRI
GIKRSIHGRMWNVGGRATVESRPGHGTTVRLEWPRG

>putative two-component system histidine kinase [NanT5]
ATGTCGATTAGCTCACTGTCGCCCGGTATCGATCCGCCGGCCTCGGCGCGCCTTCCCGGC
TCGGCCGGCCCCTGGGCGCGCGAATACGTCGCGTTCAACAGGCGGTTGGCGCTCTACGGC
CGCGCCGGGGTGATGAGCGCCTGCGCCGTGCTGGGCGTGGCGGCCATGCCGATGGGCGAG
ATGGCGCCCGCGGCGGTGGTCGCGGCGGCCGTACTCGCCTGGAGCTGGATCCATCTGCGG
CTCGCCACCACCTCGGCGATGCGGCAGAGGCCGCTGCTCGCCCTGGATCTGACGGTCATG
ACGGCGCTGTGTCTGAGCCAGCGGTTCACGATCCCCGAGGCGCAGACCACGCATGCCGGC
ACCTGGATCCTGGTGTCCGTCAGCTTCACGGCCGTGGCGTACCAGCTGATGCAGCCGCCG
CTCACCGGGATTCTGGCCACGTTATGGCTGTGCTGCGCGGATGTGGTGGGCACCGCGTTG
CACCCGAACCTCGAGTGGTCAGGTGTGCTGCGCAGTGTCATCTGGGTGGTGGTGAACACC
GCCCTGGCCCGGGCCGTGGTCCGGCTGGTGTTCAGCGAGAGCCGGGCGGCCGACGAGGCC
GCCGACCGGGCCGCGCGGGCGCGGCAGCAGGGGGAGGCGGCGGAGGCCCGCTGCGCCGCG
GAACGGGAACACCTGGCCGCGCTCCACGACACCGCCTGCGCGACCCTGCTGATCGCGTCG
GCACCATGGGCGTCGATGCGGGCGGAGACGCTGCGCGCCCAGGCCGCCAGAGATCTCCTC
CGGCTGCGCGCCGAGCAGCCGGTGGCGGGGGAGGTGGACCTGGCCGCCGAACTGCTGGAC
GAGATCGCCGCGCATCCCCTCCGCGTCATCAACAGGTTCGACGTGGAACTGGGCACGACC
TGGCGCCCCATCGCGGCAGCCCTGCGCGGCGGCCTCGGCGAGGCGCTGCGCAACGTGGCC
CGGTACGCTGGCGTCGATGCCGTACGGGTCACGGCGGAGCGCGTGGACCATGTCATCGTC
GTGACCATCGCCGACAGCGGGGTGGGGTTCGACCCGGAGCACATTCCAGGCCACCGGATC
GGGATCAAGCGTTCGATCCACGGCCGGATGTGGAACGTGGGCGGCCGCGCCACCGTCGAG
TCCCGGCCCGGCCACGGTACGACGGTGCGGCTGGAGTGGCCGCGTGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003594 ATPase-like, ATP-binding domain (Domain) G3DSA:3.30.565.10 ATP_bd_ATPase SM00387 HATPase_c PF02518 HATPase_c SSF55874 ATP_bd_ATPase
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Nanch_00040 Nanch_00060