Nanch_00120 Nanch_00120   Nanch_00140 Nanch_00140

Nanch_00130 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameNanchangmycin
Contig
Start / Stop / Direction60,305 / 65,302 / + [in whole cluster]
60,305 / 65,302 / + [in contig]
Location60305..65302 [in whole cluster]
60305..65302 [in contig]
TypeCDS
Length4,998 bp (1,665 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)NanA6
Gene
Gene (GenBank)nanA6
EC number
Keyword
Note
Note (GenBank)
  • PKS
Reference
ACC
PmId
[12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
comment
Nanchangmycin生合成のためのcomplete gene clusterの報告。
（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）

NanA6: PKS
Module 9(KS, ATa, KR, ACP)

module 9のKR domainは4つの保存されたactive site残基のうち2つが変更されているためinactive.

close this sectionPKS/NRPS Module

9 malonyl-CoA
KS35..411
AT564..883
kr1209..1386
ACP1510..1580

close this sectionSequence

selected fasta
>polyketide synthase [NanA6]
MSTTEEKLRQYLKRVTLDLGQAKQRLREAEERHQEPIAITAMACRYPGGVRSPEALWDLV
ATRTDAIGPFPTNRGWDLEGLFHPDPDHYGTSYVREGGFLHDAERFDASFFNISPREALA
MDPQQRVLLETAWELLERAHIDPHSLKGTLTGVYTGVSSQDYLSRIPRIPEGFEGYTATG
GLMSVVSGRVAYTLGLEGPAVTLDTACSASLVAMHLAGQALRQGECDLALAGGVTVFSTP
TAYVEFSRQRGFAPDARCKPFAAAADGTGFSEGVGLVLLERLSDAQRHGRRVLAVLRGSA
VNQDGASNGLSAPNDAAQERVIRQALDSARLTADQVDAVEAHGTGTTLGDPIEAQALLAT
YGKERSADRPLWLGSVKSNIGHTHAAAGVAGVIKMVMAMHHGRLPATLHVDEPTSHVDWD
TGTVRLLTEPVDWPRGDRPRRAGVSSFGISGTNAHVILEEAALPPAATGAERPGDRLTPW
VVSARGQAALHDQARRLLDATVDGDPEAVGWSLVASRAVFDQRAVITGRDTETLRAGLAA
LAAGEDHPALVRREAGVPASGSQVWLFSGQGSQRPGMGAELHERFPAFADTFDEICALID
PHLDHPLRDIVFATHPDHTDLLNHTTYTQAGLFAVQVALARLLEHCGLRPDTVIGHSIGE
ITAAHIAGVLSLQDACHLVANRATLLGKLPPGGAMTAIEATAEEITQTLTPYHGQVTIAA
LNAPTSTVISGPEELVAQLTRRWKERGRRTKTLTVSHAFHSPLMEPALNDFRHAIDHLTY
HQPTIPLISNLTGEPATQDIATPDYWVRHIRQPVHFHPAITHIAPHTAVFLEIGPDATLI
PATQNTLDTLDKQPAHPPQLIPTLTRKQPDTQALAHALARLHTLTPLNWHPWYTDQPTPT
TIDLPTYPFQRERYWLPDALADAPPPEADEEQVRFWNAVEAQDLPALSDTLGIGEEDGRR
SSLGAVLPTLSRWHQERHERATVSSWRYRVGWRHLPDLGPAAVAGPWLLVVPPKGADAWA
DACERALTADGGEVRRLVTDGRADVAELAASLRALYAEGPSPAGVLSLLPLDERPHEAFP
AVTGGVTGTHVLLRALLDAELDAPLWCATRGAVAVDDDEAPEAPAQAQVWGLGRVAALEH
PTAWGGLVDLPASVADLAPDLLCAVLAGRNGEDQVALRPAGAFGRRLLPAPLDAQAPAQE
RAWTPRDGVLVTGGVAGAAALVARWLAADGTKHIVLLAPDGPAAPGGAELVAELAELGAE
ATVVDGVPSEPTTRQELADRLAASGLRVRTVVHAGAPGDWAPLAELTPDELAEALSDAMG
GADRLAELCGLEPDDPVVVFSSIAAVWGGGGHGARAAADAYLDAWARRRQAAGGHVARLA
WGVWDGSEDPEAAERAERQGLLALHPTPALAALRRTLDHSGDGTDQGTVRAGDEGGDRSD
VHAVIADVHWDRFVPLFTMARASRLFDEIPAARRAWQAALDSSDDESSESLTALRDRLAA
QSPQARTGTLLALVRAHVAGALRYPAAESVDPEQPFKELGFDSLAAVEFRNRLRGAIGLT
LPATLVFDYPTPTALAGYLVSQVLPAEPADEPAAAHLDEIEATLAALDADDPRRAGLTHR
LRLLLWRYADGDDALEPREETGGDDLETASADEMFALIDREFGES
selected fasta
>polyketide synthase [NanA6]
GTGTCAACCACTGAAGAGAAACTGCGCCAGTACCTCAAGCGCGTCACCCTCGACCTCGGC
CAGGCCAAGCAACGCCTGCGGGAAGCGGAAGAACGCCACCAGGAACCCATCGCCATCACC
GCCATGGCCTGCCGCTACCCCGGCGGCGTCAGGTCACCGGAAGCCCTGTGGGACCTCGTC
GCCACCCGCACCGACGCCATCGGCCCTTTCCCGACCAACCGCGGCTGGGACCTGGAGGGC
CTGTTTCACCCGGACCCCGACCACTACGGCACCAGCTACGTCCGGGAAGGCGGCTTCCTC
CACGACGCCGAGCGGTTCGACGCCTCCTTCTTCAACATCAGCCCCCGCGAAGCCCTCGCC
ATGGACCCCCAGCAGCGAGTGCTCCTGGAGACCGCCTGGGAACTGCTGGAACGCGCCCAC
ATCGACCCCCACAGCCTCAAAGGCACCCTCACCGGCGTCTACACGGGGGTGTCGAGCCAG
GACTATCTGTCGCGGATACCGCGGATTCCCGAGGGTTTCGAGGGCTATACGGCCACGGGC
GGACTCATGAGCGTGGTGTCCGGCCGCGTGGCGTACACGCTGGGCCTGGAGGGACCGGCG
GTCACGCTGGACACCGCGTGCTCCGCGTCGCTGGTGGCGATGCACCTCGCCGGGCAGGCG
CTGCGGCAGGGCGAGTGCGACCTGGCCCTGGCCGGTGGTGTGACCGTGTTCAGCACGCCC
ACCGCGTATGTGGAGTTCTCCCGGCAGCGGGGGTTCGCACCGGACGCCCGCTGCAAGCCG
TTCGCCGCCGCAGCCGACGGCACCGGCTTCTCCGAGGGCGTGGGCCTGGTGCTGCTGGAG
CGGCTGTCGGACGCCCAGCGCCACGGGCGTCGTGTCCTCGCCGTGCTGCGTGGCTCGGCG
GTCAACCAGGACGGCGCGAGCAATGGGCTGTCCGCGCCCAACGACGCCGCGCAGGAGCGC
GTGATCCGGCAGGCGCTGGACAGCGCCCGGCTCACCGCCGACCAGGTGGACGCGGTCGAG
GCGCACGGCACCGGAACCACGCTCGGTGACCCCATCGAGGCGCAGGCGCTCCTTGCCACC
TACGGCAAGGAGCGTTCGGCGGACCGGCCGCTGTGGCTGGGGTCGGTGAAATCGAACATC
GGCCACACCCACGCGGCGGCGGGCGTGGCGGGCGTGATCAAGATGGTGATGGCCATGCAT
CACGGCCGGCTGCCCGCCACCCTGCACGTCGACGAGCCGACCTCCCATGTGGACTGGGAC
ACGGGCACGGTGCGACTGCTGACCGAGCCGGTCGACTGGCCGCGGGGGGACCGTCCGCGC
CGGGCAGGCGTGTCCTCGTTCGGCATCTCCGGTACCAACGCCCATGTGATTCTGGAGGAG
GCCGCCCTGCCGCCCGCCGCCACGGGCGCCGAGCGGCCGGGAGACCGGCTCACGCCGTGG
GTGGTGTCGGCGCGCGGCCAGGCCGCGCTGCACGACCAGGCCCGGCGGCTGCTCGACGCG
ACCGTTGACGGCGATCCCGAGGCAGTGGGCTGGTCGCTGGTCGCCTCGCGTGCGGTGTTC
GACCAGCGGGCGGTGATCACGGGCCGGGACACCGAAACGCTGCGGGCGGGTCTGGCCGCA
CTGGCGGCCGGGGAGGACCATCCGGCGCTGGTGCGACGTGAGGCGGGGGTACCGGCCTCG
GGGTCGCAGGTGTGGCTGTTCTCCGGGCAGGGGAGCCAGCGGCCGGGCATGGGCGCCGAA
CTCCATGAGCGTTTCCCCGCCTTCGCCGACACCTTCGACGAGATCTGCGCACTGATCGAC
CCCCACCTCGACCACCCCCTGCGCGACATCGTCTTCGCCACCCACCCCGACCACACCGAC
CTCCTGAACCACACCACCTACACCCAAGCCGGACTCTTCGCCGTACAAGTGGCACTCGCC
CGGCTCCTGGAACACTGCGGCCTACGACCCGACACCGTGATCGGCCACTCCATCGGCGAG
ATCACCGCCGCCCACATCGCCGGCGTCCTCTCCCTCCAAGACGCCTGCCACCTCGTCGCC
AACCGCGCCACCCTCCTCGGCAAACTCCCACCCGGCGGCGCCATGACCGCCATCGAAGCC
ACCGCCGAAGAAATCACCCAAACCCTCACCCCCTACCACGGCCAAGTCACCATCGCCGCC
CTCAACGCCCCCACCAGCACCGTCATCTCAGGACCCGAAGAACTCGTGGCCCAACTCACC
CGCAGATGGAAAGAACGCGGCCGCAGAACCAAAACACTCACCGTCAGCCACGCCTTCCAC
TCCCCCCTCATGGAACCCGCCCTCAACGACTTCCGCCACGCCATCGACCACCTGACCTAC
CACCAGCCCACCATCCCCCTCATCAGCAACCTCACCGGCGAACCCGCAACCCAAGACATC
GCCACCCCCGACTACTGGGTGCGCCACATCCGCCAACCCGTCCACTTCCACCCCGCCATC
ACCCACATCGCACCCCACACAGCCGTCTTCCTCGAAATCGGCCCCGACGCCACACTCATC
CCCGCCACCCAAAACACCCTCGACACCCTCGACAAACAACCCGCACACCCACCCCAACTG
ATCCCCACCCTCACCCGCAAACAACCCGACACCCAAGCCCTCGCCCACGCCCTCGCCCGC
CTCCACACCCTCACCCCCCTCAACTGGCACCCCTGGTACACCGACCAACCCACCCCCACC
ACCATCGACCTCCCCACCTACCCCTTCCAACGCGAGCGGTACTGGCTGCCCGATGCCCTC
GCGGACGCCCCGCCGCCGGAGGCGGACGAGGAGCAGGTCCGGTTCTGGAACGCGGTGGAG
GCGCAGGATCTCCCGGCTCTGTCCGACACGCTGGGCATCGGCGAGGAGGACGGGCGGCGC
TCGTCGCTCGGCGCGGTGCTGCCGACGCTGTCGCGCTGGCACCAGGAACGCCATGAGCGG
GCGACCGTGAGCTCCTGGCGCTATCGGGTGGGCTGGCGCCACCTGCCGGACCTCGGCCCG
GCGGCCGTGGCGGGGCCGTGGCTGCTGGTCGTGCCGCCAAAGGGCGCCGACGCGTGGGCC
GACGCCTGTGAGCGGGCCCTCACTGCGGACGGCGGCGAGGTGCGGCGGCTGGTGACGGAC
GGGCGGGCCGATGTGGCCGAGCTCGCCGCGTCGCTGCGGGCGCTGTACGCCGAGGGCCCG
TCCCCGGCGGGGGTGCTGTCCCTGCTGCCCCTCGACGAGCGTCCGCACGAGGCGTTCCCC
GCCGTGACCGGCGGTGTGACGGGCACCCACGTCCTGCTGCGGGCCCTGCTGGACGCCGAG
CTCGACGCGCCGCTGTGGTGCGCCACGCGGGGCGCGGTGGCGGTCGATGACGATGAAGCA
CCTGAGGCCCCGGCGCAGGCTCAGGTGTGGGGGCTGGGCCGGGTGGCCGCGCTGGAGCAT
CCGACGGCGTGGGGCGGGCTGGTCGACCTGCCGGCCTCGGTCGCGGACCTCGCTCCCGAT
CTGCTGTGCGCCGTGCTGGCGGGGCGGAACGGGGAGGACCAGGTGGCGCTGCGGCCGGCC
GGGGCCTTCGGGCGGCGGCTGCTGCCCGCTCCGCTGGATGCCCAGGCCCCGGCCCAGGAG
CGGGCGTGGACGCCCCGGGATGGCGTCCTCGTCACCGGCGGGGTCGCCGGAGCCGCGGCC
CTCGTGGCGCGGTGGCTGGCCGCGGACGGCACCAAGCACATCGTGCTGCTGGCGCCGGAC
GGCCCGGCCGCGCCCGGTGGTGCGGAACTGGTGGCCGAGCTGGCGGAGCTGGGCGCGGAG
GCGACGGTGGTGGACGGCGTGCCGTCCGAACCGACGACCCGCCAGGAGCTGGCCGACCGG
CTCGCGGCCTCGGGTCTGCGGGTCCGCACGGTCGTGCACGCCGGGGCGCCGGGCGACTGG
GCGCCGCTGGCAGAGCTCACCCCAGACGAGCTGGCCGAGGCGCTGTCCGACGCCATGGGG
GGAGCCGATCGGCTCGCGGAGCTGTGCGGCCTGGAGCCCGACGACCCGGTGGTGGTCTTC
TCGTCCATCGCCGCGGTCTGGGGCGGTGGCGGGCACGGTGCCCGGGCGGCGGCCGACGCG
TATCTCGACGCGTGGGCGCGGCGGCGCCAGGCGGCGGGCGGCCATGTCGCCCGGCTGGCG
TGGGGCGTGTGGGACGGCTCGGAAGACCCGGAGGCGGCCGAACGCGCCGAGCGTCAGGGG
CTTTTGGCCCTCCACCCGACGCCCGCGCTGGCCGCGCTGCGGCGGACGCTCGATCACAGC
GGTGACGGAACCGACCAAGGCACCGTCCGGGCCGGTGATGAGGGCGGCGACCGGAGTGAC
GTCCACGCGGTGATCGCGGATGTGCACTGGGACCGTTTCGTGCCCCTGTTCACCATGGCG
CGTGCCAGCCGCCTCTTCGACGAGATCCCGGCGGCCCGGCGGGCGTGGCAGGCGGCACTG
GACTCCTCGGACGACGAGAGTTCCGAGAGCCTGACGGCCCTGAGGGACCGGCTGGCGGCC
CAGTCGCCCCAGGCGCGCACCGGCACGCTGCTGGCCCTCGTCCGCGCCCATGTGGCCGGG
GCGCTGCGCTACCCGGCGGCGGAGTCGGTCGATCCGGAGCAGCCCTTCAAGGAGCTGGGT
TTCGACTCCCTGGCGGCCGTCGAGTTCCGGAACCGGCTACGGGGCGCCATCGGCCTGACG
CTGCCCGCCACCCTGGTCTTCGACTATCCGACGCCGACCGCGCTGGCCGGGTATCTGGTC
TCGCAGGTCCTGCCGGCCGAGCCGGCGGACGAACCCGCCGCCGCGCATCTCGACGAGATC
GAGGCGACGCTCGCCGCCCTGGACGCGGACGACCCGCGCCGCGCCGGGCTGACGCACCGA
CTGCGGTTGCTGCTGTGGCGGTACGCCGACGGGGACGATGCGCTCGAACCGCGCGAGGAG
ACGGGCGGGGACGACCTGGAAACGGCGTCCGCGGACGAGATGTTCGCCCTCATCGACCGC
GAGTTCGGGGAGTCCTGA
[9] KS35..411
[9] AT564..883
[9] malonyl-CoA757..761
[9] kr1209..1386
[9] ACP1510..1580
[9] KS103..1233
[9] AT1690..2649
[9] malonyl-CoA2269..2283
[9] kr3625..4158
[9] ACP4528..4740

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[563-689] 3.79999999999991e-75 G3DSA:3.40.366.10 [757-877] 3.79999999999991e-75 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR009081 Acyl carrier protein-like (Domain)
[1514-1579] 4.80000000000001e-13 PF00550
PF00550   PP-binding
[1510-1580] PS50075
PS50075   ACP_DOMAIN
[1503-1601] 2.39999798157265e-22 SSF47336
SSF47336   ACP_like
[1510-1583] 1.5e-22 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[1209-1386] 2.59999999999998e-36 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[35-285] 1.60000000000002e-90 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[293-411] 6.6999999999999e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[564-883] 7.30000000000007e-69 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[2-35] 2.39999798157265e-10 SSF101173
SSF101173   Polyketide_synth_docking
[3-28] 4.9e-10 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[562-865] 8.00002659346736e-67 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[691-756] 1.39999892049878e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[34-295] 9.49999999999983e-85 G3DSA:3.40.47.10 [297-461] 1.2e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[27-409] 1.50000965748778e-99 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1209-1387] 1.4e-14 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[198-214] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[566-868] 2.7000136937899e-126 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1511-1583] 8.1000073432326e-37 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[37-463] SM00825
SM00825   PKS_KS
SignalP No significant hit
TMHMM No significant hit
Nanch_00120 Nanch_00120   Nanch_00140 Nanch_00140
Page top