Nanch_00160 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	67,823 / 68,863 / + [in whole cluster] 67,823 / 68,863 / + [in contig]
Location	67823..68863 [in whole cluster] 67823..68863 [in contig]
Type	CDS
Length	1,041 bp (346 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	NanG4
Gene
Gene (GenBank)	nanG4
EC number
Keyword	4-O-methyl-L-rhodinose
Note
Note (GenBank)	NDP-D-glucose-4,6-dehydratase; NDP-D-glucose-4-epimerase; NDP-D-glucose-4-reductase
Reference	ACC Q7WTE7 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003) comment Nanchangmycin生合成のためのcomplete gene clusterの報告。（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）配列解析からの機能予測。 NanG4: NDP-D-glucose-4,6-dehydratase, NDP-D-glucose-4-epimerase, NDP-D-glucose-4-reductase 4-O-methyl-L-rhodinose生合成経路を提唱しているFig. 4では、 dTDP-4-keto-2,6-dideoxy-D-Glu ↓dehydratase dTDP-D-cinerulose ↓epimerase dTDP-L-cinerulose ↓reductase dTDP-L-rhodinose のtrifunctionが割り当てられている。
Related Reference	ACC Q9L4S7 NITE Urd_00230 PmId [11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000) comment 5th(Q9L4S7) 47%, 5e-52 Streptomyces fradiae_urdZ3 NDP-hexose 4-ketoreductase UrdZ3 [Urd_00230]putative NDP-hexose C-4 ketoreductase urdZ3, urdQ and urdZ1をそれぞれ不活化するとL-rhodinoseを産生できない株となり、主にurdamycinone B (D-olivoseあり、L-rhodinoseなし)を蓄積。各genesで相補され、urdamycin A産生回復。よってこれらのgenesはNDP-L-rhodinose生合成にのみ関与する。 urdamycin deoxysugar(NDP-D-olivose, NDP-L-rhodinose)生合成経路のFigあり。 UrdZ3 = NDP-L-cinerulose → NDP-L-rhodinose この機能的割り当てを確認するにはさらなる研究が必要。 urdR不活化株で蓄積するurdamycin MはD-rhodinoseを含むので、 UrdZ3 = NDP-D-cinerulose → NDP-D-rhodinose の還元もできるようだ。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment 9th(O33708) 46%, 4e-48 Streptomyces peucetius_dnmV thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase [Adria_00200]NDP-hexose C-4 ketoreductase --- [PMID: 9209071](1997) dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 --- [PMID: 10631513](1999) 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597](1998) abstract S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase [NanG4]
MAASPVPPPRGDEALAGTPVLVLGGSGYLGRHICSAFGAAGAQVVPVSRGARGGVDGDGC
RSVRLDLTAAGPDELARLCAGTGARVLVNASGAVWGGGERQMAEANTELVGRLAGAVARL
PGRPRLIHLGSAYEYGPARPGTAIAEDWPPAPTTVYGRTKLSGSQAVLRAAAELGVAGTV
LRVSVACGPGAPVSSLAGAVAAHLAAGRDELRLAPLRDHRDLVDVRDVADAVVAAAVAPV
AAVTGTVVNIGSGQAVPVRRLVDLMIALSGRPVRVIEDPALRRTRSDAAWQRLDIGRARR
LLGWAPRRTLRESLRDLLAAVGAPQPAAVRAATAIGPRNSHGKDSR

>putative NDP-hexose C-4 ketoreductase [NanG4]
ATGGCCGCCTCTCCGGTGCCCCCGCCCCGCGGTGACGAAGCGCTGGCCGGCACGCCGGTC
CTGGTCCTGGGCGGCTCCGGCTACCTGGGGCGGCACATATGCTCGGCGTTCGGTGCCGCG
GGCGCCCAGGTGGTGCCGGTCTCGCGCGGCGCGCGCGGCGGCGTGGACGGTGACGGCTGC
CGCTCGGTGCGCCTGGATCTGACCGCGGCCGGGCCCGATGAGCTGGCCCGGCTGTGCGCC
GGTACAGGGGCGCGGGTGCTGGTGAACGCCTCGGGCGCGGTCTGGGGCGGTGGCGAACGG
CAGATGGCCGAGGCCAACACCGAGCTGGTCGGCCGGCTGGCCGGGGCCGTCGCCCGATTG
CCGGGCCGGCCGCGGCTGATTCACCTGGGCAGCGCCTACGAGTACGGCCCGGCCCGCCCG
GGGACCGCGATCGCGGAGGACTGGCCGCCCGCCCCGACCACCGTCTACGGCCGCACCAAG
CTGAGCGGCTCGCAGGCCGTCCTTCGGGCCGCCGCCGAGCTCGGTGTCGCCGGAACCGTG
CTGCGGGTCTCCGTCGCCTGCGGCCCGGGCGCCCCGGTGAGCAGCCTCGCCGGGGCGGTG
GCCGCGCACTTGGCGGCCGGCCGCGACGAGTTGCGGCTCGCGCCCCTGCGCGACCACCGC
GACCTGGTGGACGTCCGGGACGTGGCCGACGCGGTGGTCGCGGCGGCCGTGGCACCGGTC
GCCGCCGTCACCGGCACAGTCGTCAACATCGGCAGCGGCCAGGCGGTGCCCGTGCGCCGG
CTGGTCGATCTGATGATCGCCCTCAGCGGGCGCCCGGTGCGTGTCATCGAGGACCCCGCG
CTGCGCCGGACGCGTTCCGACGCGGCCTGGCAGCGACTGGACATCGGACGTGCGCGGCGC
CTGCTGGGCTGGGCGCCGCGCCGAACCCTGCGGGAGTCACTGCGCGATCTGCTCGCCGCC
GTCGGCGCGCCGCAGCCCGCGGCGGTACGAGCGGCGACAGCGATCGGACCCCGGAACAGT
CATGGGAAGGACAGCAGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Nanch_00150 Nanch_00170