Nanch_00140 Nanch_00140   Nanch_00160 Nanch_00160

Nanch_00150 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameNanchangmycin
Contig
Start / Stop / Direction66,881 / 67,798 / + [in whole cluster]
66,881 / 67,798 / + [in contig]
Location66881..67798 [in whole cluster]
66881..67798 [in contig]
TypeCDS
Length918 bp (305 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose O-methyltransferase
Product (GenBank)NanM
Gene
Gene (GenBank)nanM
EC number2.1.1.-
Keyword
  • 4-O-methyl-L-rhodinose
Note
Note (GenBank)
  • putative methyltransferase
Reference
ACC
PmId
[12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
comment
Nanchangmycin生合成のためのcomplete gene clusterの報告。
（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）

配列解析からの機能予測。
NanM: Methyltransferase

Nanchangmycinのsugar moiety(4-O-methyl-L-rhodinose)の生合成において、NanMはdTDP-L-rhodinoseの4-O-methylationを触媒し、4-O-methyl-L-rhodinoseを生成するようだ。
Related Reference
ACC
Q2I754
PmId
[16632249] Biosynthesis of the terpene phenalinolactone in Streptomyces sp. Tu6071: analysis of the gene cluster and generation of derivatives. (Chem Biol. , 2006)
comment
2nd(Q2I754) 62%, 1e-111
Streptomyces sp. Tu6071_plaM1
PlaM1

PlaM1は、putative rhodinosyl-4-O-methyltransferase_NanMと高い相同性あり。
PlaM1 inactive mutantでは、wildの主要代謝産物phenalinolactones A and Dが両方とも産生されず、新しい代謝産物を産生する。それはphenalinolactones chromophoreの典型的なUV/VIS spectrumを示すが、HPLC/electrospray ionization (ESI)-MSによって解析したところ、methyl基がないことがわかった。さらに1H-NMR spectroscopyからその位置を同定した。
以上からPlaM1がL-amicetosyl-4''-O-methyltransferaseであることが実証された。

close this sectionSequence

selected fasta
>putative NDP-hexose O-methyltransferase [NanM]
MAQGFQASLQWERINELWVTEEASADLTGFKSDRRNFNIALWDPTTNGIRYLRALVYELA
TRLSDDDWSKIEKVRNRDVGDPVTVRYEGRTVCLDYLQAALELGFIEKELDLGGARVLEI
GAGYGRTCHAMLSNYDLASYTIVDLKNTLGLSRAYLREVLDEKQFSKMRFVQVEDIDTGL
GPDGFDLCVNVHSFTEMTPDTVKAYLRLIDERCGAFFVKNPVGKFRDKSMDGHQKGEEAV
RLAMQTGPLRQVLDIHDSQAVAAAVPAFIEAYQPGEGWTCAANTRGMPWSYFWQALYTKT
GDDLR
selected fasta
>putative NDP-hexose O-methyltransferase [NanM]
ATGGCTCAGGGTTTTCAGGCCAGCCTGCAGTGGGAACGCATCAATGAACTCTGGGTTACC
GAGGAGGCGTCGGCTGATCTGACCGGCTTCAAGTCGGACCGCCGGAATTTCAATATCGCC
CTGTGGGATCCGACGACCAACGGAATCAGGTATCTGAGGGCTCTCGTCTACGAGTTGGCC
ACGCGGCTGAGCGACGACGACTGGTCGAAGATCGAGAAAGTCCGCAACCGTGACGTGGGA
GATCCGGTCACCGTCCGATACGAGGGCAGGACCGTCTGTCTGGACTATCTCCAGGCGGCG
CTCGAGCTCGGCTTCATCGAGAAGGAACTGGACCTGGGCGGCGCCCGCGTCCTGGAGATC
GGCGCCGGGTACGGCCGTACGTGCCATGCGATGCTCTCCAACTACGACCTGGCCTCCTAC
ACCATCGTCGACCTGAAGAACACCCTCGGGCTGAGCAGGGCGTATCTTCGCGAGGTCCTG
GACGAGAAGCAGTTCTCCAAGATGAGGTTCGTCCAGGTGGAGGACATCGACACGGGGCTG
GGCCCGGACGGCTTCGACCTGTGCGTCAACGTGCACTCCTTCACGGAGATGACCCCGGAC
ACCGTCAAGGCGTATCTGCGCCTGATCGACGAGAGGTGCGGGGCGTTCTTCGTGAAGAAC
CCGGTCGGAAAGTTCCGGGACAAGAGCATGGACGGCCACCAGAAGGGCGAGGAGGCCGTG
CGGCTGGCCATGCAGACCGGGCCGCTGCGGCAGGTGCTCGACATCCATGACAGCCAGGCC
GTGGCGGCCGCCGTGCCGGCCTTCATCGAGGCCTATCAGCCCGGCGAGGGATGGACGTGC
GCCGCCAACACCCGCGGCATGCCCTGGAGTTACTTCTGGCAGGCCCTCTACACGAAGACC
GGCGACGACCTTCGATAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003333 Mycolic acid cyclopropane synthase (Family)
[113-211] 9.5e-05 PF02353
PF02353   CMAS
SignalP
[1-25] 0.103 Signal
Eukaryota   
TMHMM No significant hit
Nanch_00140 Nanch_00140   Nanch_00160 Nanch_00160
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