Nanch_00310 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	105,025 / 103,451 / - [in whole cluster] 105,025 / 103,451 / - [in contig]
Location	complement(103451..105025) [in whole cluster] complement(103451..105025) [in contig]
Type	CDS
Length	1,575 bp (524 aa)

Annotation

Category	5.1 general function
Product	putative glycosyltransferase
Product (GenBank)	NanG6
Gene
Gene (GenBank)	nanG6
EC number
Keyword
Note
Note (GenBank)	putative glycosyltransferase
Reference	ACC Q7WTD2 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003) comment nanchangmycin生合成のためのcomplete gene clusterの報告。（complete clusterと言っているが、同定されたclusterのみでNanchangmycinが生合成できるかは確認されていない。） nanG6はglycosyltransferase homologsをコードするが、deoxysugar生合成に必要なその他のgenesとcluster形成しておらず、おそらく一次代謝に関係する。付近の配列解析から、nanG6がnanchangmycin gene clusterのright borderであると示唆されている。
Related Reference	ACC Q9RP57 PmId [10531250] Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113. (Infect Immun. , 1999) comment 63th(Q9RP57) 28%, 3e-19 E.coli_wbnE WbnE rfb locus (contains a cluster of nine genes) are involved in the O113 O-antigen biosynthesis.
Family/Domain	FD IPR001296 comment [IPR001296] Glycosyl transferase, group 1 (Domain)

Sequence

>putative glycosyltransferase [NanG6]
MLGAAPVSAVSLTGVGADIWALPRVVGTQYRAAMTRTAYIRHVIARDEKVTIRWGMAVQI
GFSARPLAPRRYADRAATPTAYRRASYVIYIPPSVMVGCDSACDIRKMGPGRPMRVLHII
SRLGAGGAEQQLRLLLRHLPARCDVLTLTAPGAAGHGIRADGVPVTHLPMGVRDPMGTAR
LARFIRGGGYDLVHTHLYRACVHGRVAARLAGVGAVVATEHSLGERHIDGRPLTVGTRAR
YLATERLGSATVAVSAAVADRLRGWGVANHRIHLVPNGLDARHFSFTPAARTAARARLGI
PAHAFVVGGVGRLVAAKRFDVALRAVAELPQARLLLVGDGPERTALRDLAAWLGVADRIR
LLGERDGAVAGTGDRPADIPGLLAAMDVLVAPSAEEAFGLAALEALAAGLPGFSRRLPGP
RRTAARRGPRRPPHRARHRRPGRRPARAGRRRPRTAPGARRCRALRHHPHRPPAGPAVRT
LRQLRARRLRSRPRRHPALAAERCSRGLTPRTRLKAALSAKELP

>putative glycosyltransferase [NanG6]
GTGCTGGGCGCGGCTCCCGTCTCGGCCGTGTCTCTGACGGGGGTAGGTGCGGACATCTGG
GCACTCCCGCGGGTCGTCGGGACGCAATATCGCGCGGCGATGACCCGGACGGCCTACATT
CGTCATGTCATCGCGCGAGATGAGAAGGTAACAATCCGCTGGGGAATGGCTGTGCAAATC
GGATTTTCGGCGCGCCCCCTCGCGCCTCGCCGATATGCCGATCGGGCCGCCACTCCGACG
GCCTATCGCCGCGCGTCTTACGTGATTTACATCCCGCCGTCTGTCATGGTCGGATGTGAT
TCAGCGTGCGATATACGGAAGATGGGGCCCGGTCGCCCGATGAGAGTGCTGCACATCATC
TCGCGCCTCGGCGCGGGTGGCGCCGAACAGCAACTGCGGCTGCTGCTGCGCCACCTGCCC
GCCCGTTGTGACGTCCTCACCCTGACCGCGCCCGGGGCCGCCGGCCACGGCATCCGGGCG
GACGGTGTGCCCGTCACCCATCTGCCGATGGGTGTCCGCGACCCGATGGGGACGGCCCGG
CTGGCGCGGTTCATCCGCGGTGGCGGCTACGACCTGGTGCACACCCATCTCTACCGGGCC
TGTGTCCACGGCCGGGTCGCGGCCCGGCTGGCGGGTGTCGGCGCCGTGGTCGCCACCGAG
CACAGCCTGGGTGAGCGCCATATCGACGGCCGCCCGCTCACCGTCGGCACCCGCGCCCGC
TATCTCGCCACCGAACGACTGGGCTCGGCGACCGTCGCCGTGTCGGCCGCCGTCGCCGAC
CGGCTGCGCGGCTGGGGCGTCGCCAACCACCGCATCCACCTGGTGCCCAACGGGCTGGAC
GCCCGCCACTTCAGCTTCACCCCGGCCGCCCGCACCGCCGCCCGGGCCCGTCTCGGCATC
CCCGCACACGCCTTCGTCGTCGGCGGGGTCGGCCGGCTGGTGGCGGCCAAGCGCTTCGAT
GTGGCGCTGCGGGCCGTCGCCGAACTGCCCCAGGCCCGGCTGTTGTTGGTCGGGGACGGA
CCCGAACGCACCGCGCTGCGCGACCTGGCCGCCTGGCTCGGGGTGGCCGACCGGATCCGG
CTGCTCGGCGAGCGCGACGGGGCGGTGGCCGGGACCGGGGACCGCCCCGCGGACATCCCC
GGGCTGCTTGCCGCCATGGATGTGCTCGTCGCGCCGTCCGCGGAGGAGGCGTTCGGGCTC
GCCGCCCTGGAAGCCCTGGCGGCCGGGCTCCCGGGGTTCTCACGGCGTCTGCCCGGCCCT
CGACGAACTGCCGCCCGGCGCGGCCCCCGGCGCCCGCCGCATCGGGCCCGGCACCGCCGC
CCTGGCCGACGCCCTGCGCGAGCGGGCCGCCGCCGGCCCCGTACGGCACCCGGTGCCCGC
CGCTGTCGAGCGCTACGACATCATCCGCACCGCCCGCCGGCTGGCCCTGCTGTACGAACG
CTCCGTCAGCTGCGGGCCCGACGGCTGCGCTCGCGGCCCCGCCGTCACCCTGCGCTGGCC
GCGGAGCGGTGCTCCCGCGGGCTGACCCCGCGCACCCGCTTGAAGGCGGCGCTCAGCGCG
AAGGAGCTGCCGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001296 Glycosyl transferase, family 1 (Domain) PF00534 Glycos_transf_1
SignalP	Eukaryota
TMHMM	No significant hit

Nanch_00300 Nanch_00320