Nysta_00010 Nysta_00010   Nysta_00030 Nysta_00030

Nysta_00020 : CDS information

close this sectionLocation

Organism
StrainATCC 11455 (=NBRC 15452)
Entry nameNystatin
Contig
Start / Stop / Direction2,684 / 867 / - [in whole cluster]
2,684 / 867 / - [in contig]
Locationcomplement(867..2684) [in whole cluster]
complement(867..2684) [in contig]
TypeCDS
Length1,818 bp (605 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
ProductABC transporter ATP-binding/permease protein
Product (GenBank)NysG
Gene
Gene (GenBank)nysG
EC number
Keyword
Note
Note (GenBank)
  • putative transporter (ABC family)
Reference
ACC
PmId
[10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000)
[16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005)
comment
[PMID: 10873841](2000)
Nystatin(nys) biosynthetic gene clusterの報告。
nysG: ABC transporter
@efflux of nystatin

NysH and NysGはABC familyのtransporterに高い類似性あり。
ゆえに nystatinのATP-dependent effluxに関与しているのかも。

---
[PMID: 16251298](2005)
nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10-deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。

close this sectionSequence

selected fasta
>ABC transporter ATP-binding/permease protein [NysG]
MASPDDLEEERTAPRPARRLVGLLRPHRRSVALAVSMGVGGIVLNAFGPLLLGRVTDLIA
DGVLGGVPGPAPGIDFAAIGRLLLVLLALYVVASLFMLAQGRLVASAVWRTIHELRRDAR
EKLTRLPLRHFDRQPAGELLSRTTNDIDNLQQTLQQTLAELITSIFSLLTMLVLMLVISP
SLAVVMLLSVPVSALIAARISKRAQPHYAAQWSANGTLNAHVEEVCTGHALIKGFDRRAA
AEERFDACNDAVYRAAAKAQFASGAMEPVMMFVANLGYVLVAVIGAWKVINGTLTLGDVQ
AFILYARQFSQPIVEIASVAGRLQSGIASAQRVFTLLDAPEQAPDPLRPGTPARAEGRVE
FTDVSFRYSPDTPLIENLSLTVEPGSTVAIVGPTGAGKTTLGNLLMRFYEPDSGRILLDG
TDTATMTRDDLRSRFGLVLQDTWLFGGTIAENIAYGAPGACRADIEEAARATCADRFIRT
LPQGYDTVLDDESGTVSAGEKQLLTVARAFLARPAVLVLDEATSSVDTRTEVLIQRAMNS
LRAGRTSFVIAHRLSTIRDADLIVVMDAGRIVEQGTHDQLLCAQGLYARLHAARTHTPTA
GAAAG
selected fasta
>ABC transporter ATP-binding/permease protein [NysG]
ATGGCCTCGCCGGACGACCTTGAGGAGGAGCGGACCGCGCCCCGGCCGGCACGTCGGCTC
GTCGGCCTGCTGCGTCCGCACCGCCGCTCCGTGGCACTCGCCGTGTCGATGGGCGTCGGC
GGGATCGTGCTCAACGCGTTCGGCCCGCTGCTGCTCGGCCGGGTCACCGATCTGATCGCC
GACGGCGTGCTCGGCGGCGTCCCGGGCCCGGCGCCCGGGATCGACTTCGCCGCGATCGGC
CGGCTGCTGCTGGTCCTGCTGGCGCTCTACGTCGTGGCGTCGCTGTTCATGCTGGCGCAG
GGCCGGCTGGTGGCGTCCGCGGTGTGGCGCACCATCCACGAACTGCGCCGCGACGCCCGG
GAGAAGCTGACCCGGCTGCCGCTGCGCCACTTCGACCGGCAGCCGGCCGGTGAGCTGCTC
AGCCGCACCACCAACGACATCGACAACCTCCAACAGACCCTCCAGCAGACCCTCGCCGAG
CTGATCACCTCGATCTTCTCGCTGTTGACGATGCTGGTGCTGATGCTGGTGATCTCGCCG
TCGCTGGCCGTGGTGATGCTGCTCAGCGTGCCGGTCTCGGCGTTGATCGCGGCCCGGATC
AGCAAGCGCGCGCAGCCCCACTACGCCGCGCAGTGGTCCGCGAACGGCACCCTCAACGCC
CATGTGGAGGAGGTGTGCACCGGTCATGCGCTGATCAAGGGCTTCGACCGGCGCGCGGCG
GCCGAGGAGCGCTTCGACGCCTGCAACGACGCGGTCTACCGGGCTGCGGCCAAGGCGCAG
TTCGCCTCCGGTGCCATGGAACCGGTCATGATGTTCGTCGCCAACCTCGGCTATGTGCTG
GTGGCCGTCATCGGCGCCTGGAAGGTCATAAACGGCACCCTGACCCTCGGTGACGTGCAG
GCGTTCATCCTCTACGCCCGCCAGTTCAGCCAGCCGATCGTGGAGATCGCCTCGGTGGCG
GGCCGGCTCCAGTCCGGCATCGCCTCCGCCCAGCGCGTCTTCACCCTGCTCGACGCCCCC
GAGCAGGCCCCGGACCCGCTCCGGCCCGGCACGCCCGCCCGCGCCGAGGGCCGCGTCGAG
TTCACCGACGTGTCCTTCCGCTACTCCCCCGACACGCCCCTCATCGAGAACCTCTCGCTG
ACCGTGGAACCGGGCAGCACCGTGGCGATCGTCGGCCCCACCGGCGCCGGCAAAACCACC
CTCGGCAACCTCCTGATGCGCTTCTACGAACCGGACTCCGGCCGCATCCTCCTGGACGGC
ACCGACACCGCGACGATGACCCGCGACGACCTGCGGTCCCGCTTCGGGCTGGTGCTCCAG
GACACCTGGCTGTTCGGCGGCACCATCGCGGAGAACATCGCGTACGGCGCACCCGGTGCC
TGCCGTGCGGACATCGAGGAGGCGGCCCGCGCGACCTGTGCCGACCGCTTCATCCGCACC
CTGCCGCAGGGCTACGACACCGTGCTGGACGACGAGTCGGGCACCGTCAGCGCCGGCGAG
AAGCAGCTGCTGACCGTGGCCCGGGCCTTCCTCGCCAGACCGGCCGTCCTCGTGCTGGAC
GAGGCCACCAGCTCCGTGGACACCCGGACCGAGGTCCTGATCCAACGCGCCATGAACTCC
CTGCGGGCGGGCCGGACCAGCTTCGTGATCGCGCACCGCCTGTCCACGATCCGCGACGCG
GACCTGATCGTCGTGATGGACGCCGGCCGCATCGTCGAGCAGGGCACCCACGATCAACTC
CTGTGCGCGCAGGGCCTGTACGCCCGGCTGCACGCAGCCCGCACCCACACTCCCACCGCG
GGCGCCGCGGCCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[34-312] 6.6e-46 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[359-593] 22.642 PS50893
PS50893   ABC_TRANSPORTER_2
[399-524] 1.4e-16 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[384-570] 4.1e-15 SM00382
SM00382   ATPases associated with a variety of cellula
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[14-340] 3.4e-80 SSF90123
SSF90123   ABC transporter transmembrane region
IPR017871 ABC transporter, conserved site (Conserved_site)
[496-510] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[32-325] 52.636 PS50929
PS50929   ABC_TM1F
SignalP
[1-1] Signal-anchor
Eukaryota   
[1-34] 0.419 Signal
Bacteria, Gram-positive   
TMHMM
[31-53] Transmembrane (i-o) [77-99] Transmembrane (o-i) [165-187] Transmembrane (i-o)
Transmembrane 3   
Nysta_00010 Nysta_00010   Nysta_00030 Nysta_00030
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