Nysta_00020 Nysta_00020   Nysta_00040 Nysta_00040

Nysta_00030 : CDS information

close this sectionLocation

Organism
StrainATCC 11455 (=NBRC 15452)
Entry nameNystatin
Contig
Start / Stop / Direction4,416 / 2,662 / - [in whole cluster]
4,416 / 2,662 / - [in contig]
Locationcomplement(2662..4416) [in whole cluster]
complement(2662..4416) [in contig]
TypeCDS
Length1,755 bp (584 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
ProductABC transporter ATP-binding/permease protein
Product (GenBank)NysH
Gene
Gene (GenBank)nysH
EC number
Keyword
Note
Note (GenBank)
  • putative transporter (ABC family)
Reference
ACC
PmId
[10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000)
[16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005)
comment
[PMID: 10873841](2000)
Nystatin(nys) biosynthetic gene clusterの報告。
nysH: ABC transporter
@efflux of nystatin

NysH and NysGはABC familyのtransporterに高い類似性あり。
ゆえにnystatinのATP-dependent effluxに関与しているのかも。

---
[PMID: 16251298](2005)
nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10- deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。

close this sectionSequence

selected fasta
>ABC transporter ATP-binding/permease protein [NysH]
MLLRLLRAQLRPYAWATAALVALQLVQILGTLLLPTLGAALIDQGVVRGDGGRITELGVV
MGVVALVQIAAALGAAALAARTATAMGRDLRSALFRRILDFSAREIGRFGTPSLLTRSVN
DVQQVQNLAQTGFGIVVCAPLMCLGSVLLALRQDVPLALLLVALVLVVAVCFGLLLARMG
TLYARMQLTLDRLGRLLREAITGVRVVRSFVRDDHERARFAQTNDAFLVVSRRVGRLIAT
MLPVVLLLMNGFTVALLWTGSHRIDAGRMPIGSLSALLSYLSLILMSVVMLAFVFLSVPR
ARVCAGRIAEVLDTGSSVAPPAAPQPVRGPAGRIELCAAGYRYPGAEEPVLRDVDLTVEP
GERIAVLGSTGSGKTTLLNLVLRLADATEGAVRVGGTDVRELTAATLAAAVGFVPQRPYL
FSGTVASNLRFGRPDATDEELWEALRVAQAADFVARMPDGLDAEITQGGGNVSGGQRQRL
SLARALLRRPEIYLFDDCFSALDQATDAALRTALVPYTAGATVITVAQRISAGRDADRIV
VLDRGRVVAQGTHDVLLRTSPTYREIALSQLTEEEAAHGLAGRP
selected fasta
>ABC transporter ATP-binding/permease protein [NysH]
GTGCTGTTGAGACTTCTGCGGGCACAGCTGCGCCCCTACGCCTGGGCCACCGCCGCTCTC
GTCGCCCTTCAACTCGTCCAGATCCTGGGCACTTTGCTGCTGCCGACGCTGGGCGCCGCA
CTTATCGACCAGGGGGTGGTGCGCGGCGACGGCGGGCGCATCACCGAGCTGGGTGTGGTG
ATGGGCGTGGTGGCGCTGGTGCAGATCGCCGCCGCGCTCGGTGCGGCCGCGCTGGCCGCC
CGGACCGCGACGGCGATGGGCCGCGATCTGCGGTCCGCGCTCTTCCGCCGCATCCTGGAC
TTCTCCGCCCGCGAGATCGGCCGGTTCGGCACCCCCTCCCTGCTGACCCGGTCGGTGAAC
GACGTGCAGCAGGTGCAGAACCTCGCCCAGACCGGCTTCGGCATCGTCGTCTGCGCGCCG
CTGATGTGCCTGGGCAGCGTGCTGCTGGCGCTGCGCCAGGACGTTCCGCTGGCACTGCTG
CTGGTGGCGCTGGTGCTGGTGGTGGCGGTCTGCTTCGGCCTGCTGCTGGCCCGGATGGGC
ACCCTGTACGCGCGGATGCAGCTGACCCTGGACCGGCTGGGGCGGCTGCTGCGCGAGGCG
ATCACCGGGGTGCGGGTGGTCCGGTCCTTCGTCCGCGACGACCACGAGCGCGCGCGGTTC
GCGCAGACCAACGACGCGTTCCTCGTGGTGTCGCGGCGGGTGGGTCGGCTGATCGCGACG
ATGCTGCCGGTCGTGCTGCTGCTGATGAACGGTTTCACCGTAGCCCTGCTGTGGACCGGC
TCCCACCGGATCGACGCGGGCCGGATGCCGATCGGCTCGCTCAGCGCCCTCCTGAGCTAT
CTCTCGCTGATCCTGATGTCCGTCGTGATGCTCGCCTTCGTGTTCCTGTCGGTGCCGCGG
GCCCGGGTCTGTGCCGGCCGGATCGCGGAGGTCCTGGACACCGGGTCGAGCGTGGCGCCG
CCGGCCGCGCCGCAGCCGGTGCGCGGGCCCGCCGGCCGGATCGAACTGTGCGCGGCCGGG
TACCGCTACCCGGGCGCCGAGGAGCCCGTGCTGCGGGACGTCGACCTGACCGTCGAGCCG
GGCGAGCGGATCGCCGTGCTCGGCAGCACCGGCAGCGGCAAGACGACCCTGTTGAACTTG
GTGCTGCGGCTGGCCGACGCCACCGAGGGCGCGGTGCGGGTCGGCGGCACCGACGTCCGC
GAGCTGACCGCGGCCACGCTCGCCGCCGCCGTCGGGTTCGTGCCGCAGCGGCCGTACCTG
TTCTCCGGCACCGTGGCGAGCAATCTGCGCTTCGGGCGGCCGGACGCCACCGACGAGGAG
CTGTGGGAGGCGCTGCGGGTCGCCCAGGCGGCCGACTTCGTGGCGCGGATGCCGGACGGC
CTCGACGCGGAGATCACCCAGGGCGGCGGCAATGTCTCCGGCGGCCAGCGCCAACGCCTG
TCCCTCGCCCGGGCGTTGCTGCGCCGGCCGGAGATCTATCTCTTCGACGACTGTTTCTCG
GCGCTCGACCAGGCCACCGATGCGGCGCTGCGGACCGCGCTGGTGCCGTACACCGCGGGC
GCCACGGTGATCACCGTGGCGCAGCGGATCTCCGCCGGCCGCGACGCGGACCGGATCGTG
GTGCTGGACCGCGGGCGCGTGGTCGCCCAGGGCACCCACGACGTGCTGCTGCGCACCAGC
CCGACCTACCGCGAGATCGCGCTCTCGCAGCTGACCGAGGAGGAAGCCGCCCATGGCCTC
GCCGGACGACCTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[25-288] 1.1e-28 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[375-499] 2.2e-19 PF00005
PF00005   ABC_tran
[334-569] PS50893
PS50893   ABC_TRANSPORTER_2
IPR003593 AAA+ ATPase domain (Domain)
[360-546] 2.10000267834031e-17 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[6-313] 3.99998544139406e-57 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[472-486] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[18-300] PS50929
PS50929   ABC_TM1F
SignalP
[1-40] 0.811 Signal
Eukaryota   
TMHMM
[20-42] Transmembrane (i-o) [57-79] Transmembrane (o-i) [132-151] Transmembrane (i-o) [155-177] Transmembrane (o-i) [234-256] Transmembrane (i-o) [276-298] Transmembrane (o-i)
Transmembrane 6   
Nysta_00020 Nysta_00020   Nysta_00040 Nysta_00040
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