Nysta_00040 : CDS information

Location

Organism	Streptomyces noursei
Strain	ATCC 11455 (=NBRC 15452)
Entry name	Nystatin
Contig	AF263912
Start / Stop / Direction	4,714 / 5,748 / + [in whole cluster] 4,714 / 5,748 / + [in contig]
Location	4714..5748 [in whole cluster] 4714..5748 [in contig]
Type	CDS
Length	1,035 bp (344 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	GDP-mannose 4,6-dehydratase GDP-D-mannose dehydratase
Product (GenBank)	NysDIII
Gene	nysDIII gmd
Gene (GenBank)	nysDIII
EC number	4.2.1.47
Keyword	mycosamine
Note
Note (GenBank)	putative dGDP-mannose-4,6-dehydratase
Reference	ACC Q9L4X4 PmId [10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000) [17905880] Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455. (Appl Environ Microbiol. , 2007) comment [PMID: 10873841](2000) Nystatin(nys) biosynthetic gene clusterの報告。 nysDIII: GDP-mannose-4,6-dehydratase @mycosamine biosynthesis --- [PMID: 17905880](2007) NysDIIIがGDP-mannose→GDP-4-keto-6-deoxy-D-mannoseへ変換することを反応産物のHPLCで確認している。
Related Reference	ACC Q6T1X7 PmId [11096116] Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T. (J Biol Chem. , 2001) comment 21st(Q6T1X7) 59%, 1e-110 Aneurinibacillus thermoaerophilus_gmd GDP-mannose 4,6-dehydratase(EC 4.2.1.47) GDP-d-mannose dehydratase (Gmd) converted GDP-d-mannose to GDP-6-deoxy-d-lyxo-4-hexulose, with NADP+ as cofactor. Gmd and Rmd (GDP-6-deoxy-d-lyxo-4-hexulose reductase) required for biosynthesis of the nucleotide-activated precursor GDP-d-rhamnose.
Related Reference	ACC P93031 PmId [9050909] The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose-4,6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose. (Proc Natl Acad Sci U S A. , 1997) [12881408] Interaction of GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase with GDP-mannose-4,6-dehydratase stabilizes the enzyme activity for formation of GDP-fucose from GDP-mannose. (Glycobiology. , 2003) comment 120th(P93031) 51%, 5e-92 Arabidopsis thaliana_MUR1 GDP-mannose 4,6 dehydratase 2 -- [PMID: 9050909] The mur1 mutant is deficient in L-fucose. The recombinant protein (expressed in E. coli) exhibited GDP-D-mannose-4,6-dehydratase activity in vitro. -- [PMID: 12881408] yeastでAtFX/GER1(GDP-L-fucose synthase), MUR1 genesを発現させると、GDP-fucose合成。 MUR1のみで発現させると、GDP-Man 4,6-dehydratase活性確かめられず。 --> AtFX/GER1は、MUR1 proteinがactive formになることにも関与している、と推測。

Sequence

>GDP-mannose 4,6-dehydratase [NysDIII]
MSKRALITGITGQDGSYLAEHLLSQGYQVWGLIRGQANPRKSRVSRLASELDFIDGDLMD
QGSLVSAVDTVQPDEVYNLGAISFVPMSWQQAELVTEVNGMGVLRMLEAIRMVSGLSTSR
TVSPRGQIRFYQASSSEMFGKAAETPQRETTLFHPRSPYGAAKAYGHYITRNYRESFGMY
AVSGMLFNHESPRRGQEFVTRKISLAVARIKQGLQDKLALGNLDAVRDWGYAGDYVRAMH
LMLQQDAGDDYVIGTGQMHSVRDAVRIAFEHVGLNWEDYVVIDPDLVRPAEVEVLCADSA
KAQDRLGWKPDVDFPTLMRMMVDSDLAQVSRENQYGDVLLAANW

>GDP-mannose 4,6-dehydratase [NysDIII]
ATGTCCAAACGAGCGCTGATCACCGGAATCACCGGCCAGGACGGCTCCTATCTCGCGGAG
CACCTGCTGTCCCAGGGCTACCAGGTGTGGGGTCTGATCCGCGGCCAGGCCAATCCCCGC
AAGTCCCGGGTCAGCCGCCTCGCCTCCGAACTCGACTTCATCGACGGGGACCTGATGGAC
CAGGGCAGCCTGGTCTCCGCCGTCGACACCGTGCAGCCCGACGAGGTCTACAACCTCGGC
GCCATCTCGTTCGTGCCGATGTCCTGGCAGCAGGCCGAGCTGGTCACCGAGGTCAACGGC
ATGGGCGTGCTGCGCATGCTGGAAGCCATCCGCATGGTCAGCGGACTGTCCACCTCCCGC
ACGGTCAGCCCGCGCGGCCAGATCCGCTTCTACCAGGCGTCCAGCTCGGAGATGTTCGGC
AAGGCCGCCGAGACGCCGCAGCGCGAGACCACCCTCTTCCACCCGCGCAGCCCCTACGGC
GCGGCAAAGGCGTACGGGCACTACATCACCCGCAACTACCGCGAGTCCTTCGGCATGTAC
GCGGTCTCCGGCATGCTCTTCAACCACGAATCCCCGCGCCGCGGCCAGGAATTCGTCACC
CGCAAGATCAGCCTGGCGGTCGCCCGCATCAAGCAAGGCCTCCAGGACAAGCTGGCACTC
GGCAACCTCGACGCGGTGCGCGACTGGGGCTATGCCGGCGACTACGTCCGCGCCATGCAC
CTGATGCTCCAGCAGGACGCCGGCGACGACTACGTCATCGGCACCGGGCAGATGCACTCG
GTGCGCGACGCGGTTCGGATCGCGTTCGAACACGTCGGCCTGAACTGGGAGGACTACGTC
GTCATCGACCCCGACCTGGTGCGGCCCGCCGAGGTCGAGGTGCTGTGCGCCGACAGCGCC
AAGGCCCAGGACCGCCTCGGCTGGAAGCCGGACGTCGACTTCCCCACCCTCATGCGCATG
ATGGTCGATTCCGACCTGGCGCAGGTTTCCCGCGAAAACCAATACGGCGACGTGCTGCTC
GCCGCCAACTGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Nysta_00030 Nysta_00050