Nysta_00060 Nysta_00060   Nysta_00080 Nysta_00080

Nysta_00070 : CDS information

close this sectionLocation

Organism
StrainATCC 11455 (=NBRC 15452)
Entry nameNystatin
Contig
Start / Stop / Direction50,747 / 56,947 / + [in whole cluster]
50,747 / 56,947 / + [in contig]
Location50747..56947 [in whole cluster]
50747..56947 [in contig]
TypeCDS
Length6,201 bp (2,066 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)NysK
Gene
Gene (GenBank)nysK
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase/thioesterase; contains a C-terminal thioesterase domain
Reference
ACC
PmId
[10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000)
comment
Nystatin(nys) biosynthetic gene clusterの報告。
nysK: Type I PKS
@Nystatin PKS(modules 18+TE)

module18はintact DH domainを持つが、DH domainの活性はlast condensation stepに必要ではない。
TE domainは最後の伸長unitの縮合と、PKS complexからのmature nystatin polyketide chain放出に関与。

close this sectionPKS/NRPS Module

18 malonyl-CoA
KS34..407
AT567..878
dh929..1096
KR1416..1596
ACP1696..1766
TE1854..2058

close this sectionSequence

selected fasta
>polyketide synthase [NysK]
MPDEKKLVDYLKWVTKDLHQTRQRLQEVEAGRHEPVAIVGMACRFPGGVRSPEDLWELLS
AGRDGIGPFPADRGWDLAALAGDGPGRSATQEGGFLPDAAAFDPGFFDISPREALAMDPQ
QRLLLETAWEAVERSGIDPAGLRGSRTGVFVGTNGQDYAHLVLAAQDDMGGYAGNGLAAS
VLSGRLAFALGLEGPAVTLDTACSSSLVTLHLAAQAVRAGECGLALAGGVTVMTTSSSFA
GFSLQGGLAPDGRCKAFAEAADGTGWSEGIGLLLVERLSDAQRNGHPVLAVLRGSAVNQD
GASNGLSAPNGPSQQRVIRQALAGAGLVPGDVDAVEAHGTGTRLGDPIEAGALLATYGQD
RPADRPLWLGSVKSNLGHTQAAAGVAGVIKMVLALRHGVLPQTLHVDAPSSHVDWESGAV
RLLTAPVAWSEGDDRVRRAGVSSFGISGTNAHVILEQAPDQPEPTAEETAAAAPGGTAEE
RAAAPVAPRAVPWPVAARTAGALDAQLVRVRALTTAPGRTAADVGHALATARTPFEHRAL
LVHEGGAVTEVARGAVPTGDRGGLAVLFSGQGSQRPGMGRELHARYPVFAAAFDETVALL
DARLGTSLRDIVWDQDRTRLDDTRHTQPALFAVEVALYRLLASWGIRPDHVTGHSIGEIT
AAHVAGVLTLADACTLVAARATAMSELPPGGAMVALEATEDEVRPLLTDDLAIAAVNAPR
SVVVAGAEDAALAVRRHFDDLGRRTTRLPVSHAFHSPLMDPMLDAFRTALAPLTFAEPEI
PVVSNLTGLPATAEELATPHYWVCHVRQAVRFGDGVRALADRGVRTFLELGPDGVLSALV
RENLPEPGLVAVPVLRKERPEETTVLAALGTLWAHGADVDWDAVFAGTRTPQADPVELPT
YAFQRARYWPTLGARHGDPADLGQTAAAHPLLGAAVTLADADETVLTGRLALPSHPWLGD
HRSDGRITVPGVAFAELAVRAGDLSGTPHLARLDLPAPLTLGDGDTVTLQVRVGAPDPAG
HRPLTVHARLAATEDAPWTTCATGLLAPDAPEAPADPIGPADAGWPPRDARPVPVADLDA
AATAAGRHYGPHFQGLTGLWRRDGEVFAEVALPTATAADRAFGIHPALLATALRATAALD
DDHTAGHTPEPTGITGLALHATGATALRVRLTATGPDTVALAAADATGGAVLTADTVTLG
SPQDRPAPAPAGHTGQGGLFHLDWVPVDPGSRATGTRWAVVGDDELDLGYALHRADETVS
AYAASLGGAIGDSGLAPDVFLVPVVGGPDAGPDAVHAVTARALGLLQEWLNEPRLAGARL
VFVTRGAVAVPGETVTDPAGAAVWGLLRSAQTENPGSLLLVDLDDAFRSAGMLPHVLTLD
EQQLVVRDHAVRAARLARLPEPAAGTAPARAWDPDGTVLITGGTGGLGAALARHLVTVRG
ARHLLLAGRRGPEAPGAGELVAELTAQGADVRVAACDVGDRTALDALLATVPAAHPLTAV
VHTAGVLDDALIGSLTPDQLATVLRPKADAAWHLHDATRGLDLAGFVLYSSVSGVLGSPG
QGNYAAANAYLDALARHRADQGLPALSLAWGPWGRGSGMTASVSDADLERMARGGLPPLT
VEDGLALFDAAVGRPEPALVPSRINVAGLRDQQALPALWRDLVPRARRTAATADRSPVTV
RERLRHLDETGQEQLLIDLVVGYTAGLLGHPDPTAVDPERGFLELGFDSLVSVGLRNQLA
EILGLRLPSSIVFDSKSPVKLARWLHQELANGPQPGATGPAAADARPAVRSDDTLEGLFY
NAVRGGKLVEAMRMLKAVANTRPMFDTPAELEELSEPVTLADGPGRPRLIFVSAPGATGG
VHQYARIAAHFRGSRHVSALPLMGFAPGELLPATSEAAARIVAESVLMASEGEPFVMVGH
STGGSLAYLAAGVLEDTWDVRPEAVVLLDTASIRYNPGEGNDLDRTTRFYLADIDSPSVT
LNSARMSAMAHWFMAMTDIQAPAPTAPTLLVRAARALDGFRLDTSSVPADEVRDIDADHL
SLAKEHSALTAQAIEGWLAELPDPAA
selected fasta
>polyketide synthase [NysK]
ATGCCGGACGAAAAGAAGCTCGTCGACTATCTGAAGTGGGTCACGAAGGACCTCCACCAG
ACCCGCCAGCGCCTTCAGGAGGTGGAGGCGGGGCGCCACGAACCCGTGGCGATCGTCGGC
ATGGCCTGCCGCTTCCCCGGCGGTGTGCGCTCCCCGGAGGACCTGTGGGAGCTGCTGTCC
GCGGGCCGGGACGGCATCGGGCCGTTCCCCGCCGACCGCGGCTGGGACCTGGCGGCGCTG
GCCGGCGACGGGCCCGGTCGCAGCGCCACCCAGGAAGGCGGGTTCCTGCCCGACGCGGCC
GCCTTCGACCCGGGCTTCTTCGACATCTCCCCGCGCGAGGCGCTCGCCATGGACCCGCAG
CAGCGGCTGCTGCTGGAGACCGCCTGGGAGGCCGTCGAACGCTCCGGCATCGACCCGGCC
GGGCTGCGCGGCAGCCGCACCGGCGTTTTCGTCGGCACCAACGGCCAGGACTACGCGCAC
CTGGTCCTCGCCGCGCAGGACGACATGGGCGGCTACGCGGGCAACGGCCTGGCCGCCAGC
GTGCTCTCCGGCCGACTGGCCTTCGCGCTCGGCCTGGAAGGCCCGGCCGTCACCCTCGAC
ACCGCCTGCTCCTCGTCACTGGTGACCCTGCACCTGGCCGCACAGGCCGTGCGCGCCGGC
GAATGCGGCCTCGCCCTGGCCGGTGGCGTCACGGTCATGACGACCTCGTCGAGCTTCGCC
GGCTTCAGCCTCCAGGGCGGCCTGGCGCCGGACGGCCGCTGCAAGGCGTTCGCCGAGGCG
GCCGACGGCACCGGCTGGTCCGAGGGCATCGGCCTGCTTCTCGTCGAGCGGCTCTCCGAC
GCGCAGCGCAACGGCCACCCGGTGCTCGCCGTGCTGCGCGGCTCCGCCGTCAACCAGGAC
GGCGCGTCCAACGGCCTCAGCGCGCCCAACGGTCCGTCCCAGCAGCGGGTCATCCGCCAG
GCGCTGGCCGGCGCCGGACTCGTCCCCGGCGACGTGGACGCGGTCGAGGCGCACGGCACC
GGCACCCGGCTCGGCGACCCCATCGAGGCCGGTGCGCTGCTCGCCACCTACGGCCAGGAC
CGGCCCGCCGACCGGCCGTTGTGGCTCGGCTCGGTGAAGTCCAACCTCGGCCACACCCAG
GCCGCCGCGGGCGTCGCCGGCGTCATCAAGATGGTGCTGGCCCTGCGGCATGGCGTCCTC
CCGCAGACCCTGCACGTGGACGCGCCCTCCTCGCACGTCGACTGGGAGAGCGGCGCGGTG
CGGCTGCTCACCGCACCCGTCGCCTGGTCCGAGGGCGACGACCGGGTGCGCCGGGCCGGC
GTCTCGTCGTTCGGCATCAGCGGCACCAACGCCCACGTCATCCTCGAACAAGCCCCCGAT
CAGCCGGAACCGACCGCGGAAGAGACGGCTGCCGCGGCGCCCGGCGGCACCGCCGAGGAG
CGGGCCGCCGCTCCCGTCGCCCCGCGCGCCGTGCCGTGGCCGGTCGCGGCACGCACCGCC
GGCGCCCTCGACGCCCAACTGGTCCGGGTCCGCGCGCTGACCACCGCGCCCGGCCGCACC
GCCGCCGACGTCGGTCACGCGCTGGCCACCGCCCGTACCCCCTTCGAGCACCGGGCGCTG
CTGGTCCACGAGGGCGGCGCCGTCACCGAGGTGGCGCGCGGCGCCGTCCCCACCGGTGAC
CGGGGCGGGCTGGCCGTGCTGTTCTCCGGACAGGGCTCCCAACGGCCGGGCATGGGGCGC
GAACTCCACGCCCGCTACCCGGTCTTCGCCGCCGCCTTCGACGAGACCGTCGCCCTGCTC
GACGCCCGGCTCGGCACGTCGCTGCGCGACATCGTCTGGGACCAGGACCGCACCCGGCTC
GACGACACCCGCCACACCCAGCCCGCGCTGTTCGCCGTCGAGGTCGCGCTGTACCGCCTG
CTGGCCTCCTGGGGCATCCGGCCCGACCACGTCACCGGACACTCCATCGGCGAGATCACC
GCGGCGCACGTCGCCGGTGTGCTGACCCTCGCGGACGCCTGCACCCTGGTGGCCGCCCGC
GCCACCGCCATGAGCGAACTGCCGCCCGGCGGCGCCATGGTGGCGCTGGAGGCCACCGAG
GACGAGGTGCGTCCGCTGCTCACCGACGACCTCGCGATCGCCGCGGTCAACGCCCCCCGG
TCCGTGGTCGTCGCCGGCGCCGAGGACGCCGCCCTCGCCGTCCGCCGGCACTTCGACGAC
CTGGGCCGCCGGACCACCCGGCTCCCGGTCAGCCACGCCTTCCACTCGCCGCTCATGGAC
CCGATGCTCGACGCCTTCCGGACGGCCCTCGCCCCGCTGACCTTCGCCGAGCCGGAGATC
CCGGTCGTCTCCAACCTCACCGGCCTCCCGGCCACCGCCGAGGAACTCGCCACCCCGCAC
TACTGGGTGTGCCACGTCCGGCAGGCCGTCCGCTTCGGCGACGGCGTGCGCGCCCTCGCC
GACCGCGGCGTGCGGACCTTCCTCGAACTCGGCCCGGACGGCGTGCTGTCCGCCCTGGTC
CGGGAGAACCTCCCCGAGCCGGGCCTGGTCGCCGTGCCCGTGCTGCGCAAGGAGCGGCCC
GAGGAGACCACCGTGCTGGCCGCCCTGGGAACCCTGTGGGCGCACGGCGCGGACGTGGAC
TGGGACGCGGTGTTCGCCGGCACCCGCACCCCGCAGGCCGACCCCGTCGAGCTGCCGACG
TACGCCTTCCAACGCGCCCGCTACTGGCCCACCCTCGGCGCCCGCCACGGCGACCCGGCC
GACCTCGGGCAGACCGCCGCCGCCCACCCGCTGCTGGGCGCCGCCGTCACCCTCGCCGAC
GCCGACGAGACCGTGCTCACCGGCCGCCTCGCGCTGCCCTCCCACCCCTGGCTCGGCGAC
CACCGCAGCGACGGCCGGATCACCGTCCCCGGCGTCGCCTTCGCCGAACTCGCCGTCCGC
GCCGGCGACCTGAGCGGCACCCCGCACCTGGCGCGGCTCGACCTGCCGGCGCCGCTCACC
CTCGGCGACGGCGACACCGTCACCCTCCAGGTCCGGGTCGGCGCCCCCGACCCCGCGGGC
CACCGGCCGCTGACCGTCCACGCCCGCCTCGCAGCCACCGAGGACGCCCCCTGGACCACC
TGCGCGACCGGTCTGCTCGCCCCGGACGCCCCCGAAGCGCCCGCGGATCCGATCGGCCCG
GCCGACGCCGGGTGGCCGCCGCGGGACGCCCGCCCGGTGCCCGTCGCCGACCTCGACGCG
GCCGCCACCGCCGCAGGCCGCCACTACGGCCCCCATTTCCAGGGCCTGACCGGGCTCTGG
CGGCGCGACGGCGAGGTCTTCGCCGAGGTGGCCCTGCCCACCGCCACCGCCGCCGACCGC
GCCTTCGGCATCCACCCCGCGCTGCTGGCCACCGCGCTCCGCGCCACCGCCGCACTGGAC
GACGACCACACCGCCGGCCACACCCCCGAACCGACCGGCATCACCGGACTCGCCCTGCAC
GCCACCGGGGCCACCGCACTGCGGGTCCGGCTCACCGCGACCGGGCCCGACACCGTGGCC
CTCGCCGCCGCGGACGCCACGGGCGGCGCGGTCCTGACCGCCGACACCGTCACCCTCGGC
TCCCCGCAGGACCGCCCGGCTCCCGCACCGGCCGGCCACACCGGGCAGGGCGGCCTGTTC
CACCTCGACTGGGTGCCGGTCGACCCCGGCAGCCGAGCCACCGGCACCCGCTGGGCCGTC
GTCGGCGACGACGAACTCGACCTCGGCTACGCCCTGCACCGCGCCGACGAGACGGTCAGT
GCCTACGCGGCGTCGCTGGGCGGAGCCATCGGCGACAGCGGTCTGGCGCCCGACGTCTTC
CTCGTCCCCGTCGTCGGCGGCCCGGACGCCGGGCCCGACGCGGTGCACGCCGTCACCGCC
CGCGCCCTGGGGCTGCTCCAGGAGTGGCTGAACGAGCCGCGGTTGGCCGGCGCCCGCCTG
GTCTTCGTCACCCGCGGCGCCGTCGCGGTGCCCGGCGAGACCGTCACCGACCCGGCCGGC
GCCGCCGTCTGGGGCCTGCTGCGCTCCGCCCAGACCGAGAACCCGGGCAGTCTGCTGCTG
GTCGACCTCGACGACGCGTTCCGGTCCGCCGGGATGCTGCCGCACGTCCTCACCCTCGAC
GAACAGCAGCTCGTCGTCCGCGACCACGCGGTCCGCGCCGCCCGCCTGGCCCGGCTGCCG
GAGCCGGCCGCCGGCACCGCGCCGGCCCGCGCCTGGGACCCGGACGGCACCGTCCTGATC
ACCGGCGGCACCGGCGGCCTGGGCGCCGCGCTCGCCCGCCACCTGGTCACCGTCCGCGGC
GCCCGCCACCTGCTGCTCGCCGGCCGCCGCGGCCCCGAGGCGCCGGGCGCCGGCGAACTG
GTGGCGGAGCTGACCGCACAGGGCGCGGACGTGCGGGTGGCCGCCTGCGACGTCGGCGAC
CGCACCGCCCTCGACGCGCTCCTCGCCACGGTCCCCGCGGCGCACCCGCTGACCGCCGTC
GTGCACACCGCCGGCGTCCTGGACGACGCCCTGATCGGCTCGCTCACCCCCGACCAACTG
GCCACCGTGCTACGGCCCAAGGCCGACGCCGCCTGGCATCTGCACGACGCCACCCGCGGC
CTCGACCTGGCCGGCTTCGTCCTGTACTCCTCGGTCTCCGGCGTCCTGGGCAGCCCCGGC
CAGGGCAACTACGCCGCCGCCAACGCCTACCTCGACGCGCTCGCCCGGCACCGCGCCGAC
CAGGGCCTCCCGGCGCTCTCCCTCGCCTGGGGCCCCTGGGGTCGGGGCAGCGGCATGACC
GCGTCGGTCAGCGACGCCGACCTGGAGCGGATGGCGCGCGGCGGCCTGCCGCCGCTGACC
GTCGAGGACGGCCTGGCCCTGTTCGACGCCGCCGTCGGCCGCCCCGAGCCGGCCCTGGTG
CCCAGCCGCATCAACGTCGCCGGCCTGCGGGACCAGCAGGCACTGCCGGCACTCTGGCGC
GACCTGGTACCGCGGGCCCGCCGCACCGCGGCCACCGCCGACCGCTCCCCGGTCACGGTG
CGCGAGCGCCTCCGCCACCTCGACGAGACCGGCCAGGAGCAGCTGCTCATCGACCTCGTC
GTCGGCTACACCGCCGGCCTGCTCGGCCACCCCGACCCCACCGCCGTCGATCCCGAACGG
GGCTTCCTGGAGCTGGGCTTCGACTCCCTGGTCTCGGTCGGCCTGCGCAACCAGCTCGCC
GAGATCCTCGGCCTGCGCCTGCCCTCGTCCATCGTCTTCGACAGCAAGTCGCCGGTGAAG
CTGGCGCGTTGGCTGCACCAGGAACTCGCCAACGGCCCCCAGCCGGGCGCCACCGGCCCC
GCCGCCGCGGACGCCCGTCCCGCGGTGCGCTCCGACGACACCCTGGAGGGCCTGTTCTAC
AACGCGGTGCGCGGCGGCAAGCTCGTCGAGGCGATGCGGATGCTCAAGGCCGTCGCCAAC
ACCCGGCCGATGTTCGACACCCCCGCCGAGCTGGAGGAGCTCTCCGAGCCGGTGACGCTC
GCCGACGGCCCGGGCCGGCCCCGGCTGATCTTCGTCAGCGCCCCGGGCGCCACCGGCGGC
GTCCACCAGTACGCGCGCATCGCCGCGCACTTCCGCGGCAGCCGCCATGTCTCCGCGCTG
CCCCTGATGGGCTTCGCCCCCGGCGAGCTCCTCCCGGCCACCAGCGAGGCCGCGGCCCGT
ATCGTCGCCGAGAGCGTCCTGATGGCCAGCGAGGGCGAACCGTTCGTCATGGTCGGCCAC
TCCACCGGCGGCTCGCTGGCCTACCTCGCCGCCGGCGTCCTGGAGGACACCTGGGACGTC
CGGCCCGAAGCGGTGGTCCTCCTCGACACCGCGTCCATCCGCTACAACCCCGGCGAGGGC
AACGACCTGGACCGCACCACGAGGTTCTACCTGGCCGACATCGACTCGCCCTCGGTGACG
CTCAACAGCGCCCGGATGTCCGCCATGGCCCACTGGTTCATGGCGATGACCGACATCCAG
GCGCCCGCACCGACCGCCCCCACCCTCCTCGTGCGCGCCGCCCGGGCCCTCGACGGCTTC
CGGCTCGACACCTCGTCCGTCCCCGCCGACGAGGTCCGGGACATCGACGCCGACCACCTC
TCCCTCGCCAAGGAGCACTCGGCACTGACCGCGCAGGCCATCGAGGGATGGCTCGCGGAA
CTGCCGGACCCCGCGGCCTGA
[18] KS34..407
[18] AT567..878
[18] malonyl-CoA752..756
[18] dh929..1096
[18] KR1416..1596
[18] ACP1696..1766
[18] TE1854..2058
[18] KS100..1221
[18] AT1699..2634
[18] malonyl-CoA2254..2268
[18] dh2785..3288
[18] KR4246..4788
[18] ACP5086..5298
[18] TE5560..6174

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[1854-2058] 3.8e-14 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
[562-687] 9.60000000000004e-82 G3DSA:3.40.366.10 [752-868] 9.60000000000004e-82 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1416-1583] 2.70000000000002e-65 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
[1724-1739] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1696-1770] 6.60000000000002e-14 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1696-1766] PS50075
PS50075   ACP_DOMAIN
[1704-1765] 2.1e-08 PF00550
PF00550   PP-binding
[1688-1783] 5.69999708783953e-18 SSF47336
SSF47336   ACP_like
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[34-281] 3.99999999999998e-90 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[289-407] 2.70000000000002e-48 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[567-878] 1.29999999999998e-64 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-27] 4.00000000000001e-14 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[563-862] 2.99998750445706e-76 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[689-751] 3.00000067992871e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[36-293] 5.99999999999995e-88 G3DSA:3.40.47.10 [294-461] 1.09999999999999e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[26-460] 1.19999063421924e-99 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1416-1629] 1e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[194-210] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[567-859] 9.09992432541739e-123 SM00827
SM00827   PKS_AT
IPR020802 Polyketide synthase, thioesterase domain (Domain)
[1850-2058] 5.10003242202155e-88 SM00824
SM00824   PKS_TE
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1697-1769] 4.70000326671387e-24 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[929-1096] 3.99998544139406e-59 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[36-460] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1416-1596] 1.49999648196323e-60 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Nysta_00060 Nysta_00060   Nysta_00080 Nysta_00080
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