Nysta_00070 Nysta_00070   Nysta_00090 Nysta_00090

Nysta_00080 : CDS information

close this sectionLocation

Organism
StrainATCC 11455 (=NBRC 15452)
Entry nameNystatin
Contig
Start / Stop / Direction57,095 / 58,279 / + [in whole cluster]
57,095 / 58,279 / + [in contig]
Location57095..58279 [in whole cluster]
57095..58279 [in contig]
TypeCDS
Length1,185 bp (394 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)NysL
Gene
Gene (GenBank)nysL
EC number
Keyword
  • C-10 hydroxylate
Note
Note (GenBank)
  • P450 monooxygenase
Reference
ACC
PmId
[10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000)
[16597951] Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei. (Appl Environ Microbiol. , 2006)
comment
[PMID: 10873841](2000)
Nystatin(nys) biosynthetic gene clusterの報告。
nysL: P450 monooxygenase
@hydroxylation at C-10

---
[PMID: 16597951](2006)
nysL geneが遺伝子置換によって不活化されてできたmutantは、10-deoxynystatinを産生(抗真菌作用は保たれている)。E.coliでnysLを発現させて10-deoxynystatinとの酵素活性測定。この結果から、NysLはposition C-10において10-deoxynystatinのpost-polyketide synthase modificationを担うhydroxylaseであるとわかった。

close this sectionSequence

selected fasta
>cytochrome P450 [NysL]
MSTPTAPPSLKAEVPPVLRLSPLLRELQSRAPVCKVRTPAGDEGWLVTRHTELKQLLHDD
RLARAHADPANAPRYVHNPFLDLLVVDDFDLARTLHAEMRSLFTPQFSARRVMDLTPRVE
ALAEGVLAHFVAQGPPADLHNDFSLPFSLSVLCALIGVPAEEQGKLIAALTKLGELDDPA
RVQEGQDELFGLLSGLARRKRITPEDDVISRLCLKVPSDERIGPIASGLLFAGLDSVASH
IDLGTVLFIQHPDQLAAALADEKLMRGAVEEILRSAKAGGSVLPRYATADVPIGDVTIRA
GDLVLLDFTLVNFDRTVFDEPELFDIRRAPNPHLTFGHGMWHCIGAPLARVNLRTAYTLL
FTRLPGLRLVRPVEELRVLSGQLSAGLTELPVTW
selected fasta
>cytochrome P450 [NysL]
ATGAGCACACCGACCGCACCGCCCTCCCTGAAAGCGGAGGTGCCGCCCGTCCTGCGCCTG
AGCCCGCTGCTGCGCGAACTCCAGTCCCGCGCCCCCGTCTGCAAGGTCCGCACCCCCGCC
GGCGACGAGGGCTGGCTGGTGACCCGGCACACCGAACTCAAGCAGCTGCTGCACGACGAC
CGGCTGGCCCGCGCCCACGCCGACCCGGCCAACGCCCCGCGTTATGTGCACAACCCGTTC
CTGGACCTGCTCGTCGTCGACGACTTCGACCTGGCCCGCACGCTGCACGCCGAGATGCGC
TCCTTGTTCACCCCGCAGTTCTCGGCCCGCCGCGTCATGGACCTGACGCCGAGGGTGGAA
GCCCTCGCCGAGGGGGTACTGGCCCACTTCGTCGCCCAGGGACCGCCCGCCGACCTGCAC
AACGACTTCTCGCTGCCGTTCTCCCTGTCGGTGCTGTGCGCGCTCATCGGCGTCCCGGCC
GAGGAACAGGGGAAGCTGATCGCCGCCCTCACCAAACTGGGCGAACTCGACGACCCGGCA
CGCGTCCAGGAAGGCCAGGACGAGCTGTTCGGCCTGCTGTCCGGCCTGGCACGCCGCAAG
CGCATCACACCCGAGGACGACGTCATCTCCCGGCTCTGCCTGAAGGTGCCCTCCGACGAG
CGCATCGGCCCGATCGCCTCCGGTCTGCTCTTCGCCGGCCTGGACAGCGTCGCCAGCCAC
ATCGACCTGGGCACGGTGCTGTTCATCCAGCACCCGGACCAGCTCGCCGCGGCCCTGGCC
GACGAGAAGCTGATGCGCGGCGCCGTCGAGGAGATCCTGCGGTCCGCCAAGGCCGGCGGT
TCGGTGCTCCCGCGGTACGCGACCGCCGATGTACCGATCGGCGACGTGACCATCAGGGCC
GGCGACCTGGTGCTGCTGGACTTCACCCTGGTGAACTTCGACCGCACGGTCTTCGACGAG
CCGGAGCTCTTCGACATCCGGCGCGCCCCCAACCCGCACCTGACGTTCGGCCACGGCATG
TGGCACTGCATCGGCGCGCCGCTGGCCCGGGTCAATCTGCGCACCGCCTACACCCTGCTG
TTCACCCGCCTGCCCGGCCTGCGGCTGGTGCGCCCGGTCGAGGAACTGCGGGTGCTGTCG
GGGCAGTTGTCGGCCGGCCTGACGGAGCTGCCCGTCACCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[11-394] 2.7000136937899e-71 SSF48264
SSF48264   Cytochrome_P450
[19-394] 1.10000000000001e-80 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[260-362] 2.3e-14 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[138-154] 5.00000909915354e-34 PR00359 [155-170] 5.00000909915354e-34 PR00359 [191-213] 5.00000909915354e-34 PR00359 [267-278] 5.00000909915354e-34 PR00359 [285-312] 5.00000909915354e-34 PR00359 [313-328] 5.00000909915354e-34 PR00359 [334-343] 5.00000909915354e-34 PR00359 [343-354] 5.00000909915354e-34 PR00359
PR00359   BP450
SignalP No significant hit
TMHMM No significant hit
Nysta_00070 Nysta_00070   Nysta_00090 Nysta_00090
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