Nysta_00190 : CDS information

Location

Organism	Streptomyces noursei
Strain	ATCC 11455 (=NBRC 15452)
Entry name	Nystatin
Contig	AF263912
Start / Stop / Direction	117,033 / 119,816 / + [in whole cluster] 117,033 / 119,816 / + [in contig]
Location	117033..119816 [in whole cluster] 117033..119816 [in contig]
Type	CDS
Length	2,784 bp (927 aa)

Annotation

Category	4.1 transcriptional regulator
Product	LuxR family transcriptional regulator
Product (GenBank)	NysRIII
Gene
Gene (GenBank)	nysRIII
EC number
Keyword
Note
Note (GenBank)	transcriptional regulator
Reference	ACC Q9L4V9 PmId [10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000) [14973031] In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis. (J Bacteriol. , 2004) comment [PMID: 10873841](2000) Nystatin(nys) biosynthetic gene clusterの報告。 nysRIII: transcriptional activator @regulation of nystatin production nysRI, nysRII and nysRIIIは、Streptomyces sp. SA-COOのcholesterol oxidase-cytochrome P450 operonの近くにあるtranscriptional regulatorsに相同である。 --- [PMID: 14973031](2004) regulatory genes候補の不活化mutantにおいて、nystatin gene cluster内にあるputative promoter regionsの調節下に置いたxylE reporter gene活性を測定。不活化mutantのCross-complementation experiments。 nysRIII mutantは、nysRII自身で98%まで、nysRIVで87%まで相補できる。 nysRI, II, IIIがないとnysRIV promoterには作用がない。 NysRI, II, III IVは効率的なnystatin産生のために不可欠である。

Sequence

>LuxR family transcriptional regulator [NysRIII]
MLLERENELARIRAALDAAEAGDSSLLLINGPLGSGRSALLRRIPELAGDGTRVLRASAA
WRERDFPFGIARQLFDHLLSGAGGAGPAERTAGAEHFSRLMDTGDRPTGTGPALEVSQAV
LQGAQALLADASAERRLLILVDDLQWADGPSLRWLAHLTRRLHGLRALLVCTLADGDHRG
RYPLVREVAGAAHTVLRLAPLSRDATRVLLAGPQGRPPQDALVRAVYEASRGNPLFLTAF
RSALRATGRPPGGDHFGAVRELSPTVLRDRLAGHLRIQPQPVREVAVAVAALGDHSDPVL
LAQLAGVDEIGFAGARRALVDAGLLARGRDVRFVHGVVRDAVDSLLTLDERERSHDDAAD
LLYRCGRPAEQVAGHLLAVVHPGRPWSEAVLRSAAHNALRAGRPADAARYLRRALLHHRT
QDGCRARILVDLATAERALDPDACVRHVSQAVALLDTSRDRAAAVLRIPPSLLAAPSPSA
VELVRQAAAGLDEPGQRDEEGADELALRLEAWLRHSGHENPVELASSVARLRRMGARPPV
DSVAERELVAVLLSAGALSGRLSAAEIADTGNRILEREPATAAHAHTPLPLVMLSLFVAE
SVQGVASWLASEQHTRRRYATGADDVLLTAERAFVLVTQGRPAAAREHVERALVMDAGDW
SEPAVMMFAAVAFELRDPALSERILERIRDRRPAGLALTATGQMLQAAVDVHFGRGRDAL
DTLLACGRRLETVGWRNSALLPWRPYAIGLHQRLGETDAALQLAEDELRWAREWGATTNL
GRALRLKGWLLQDEGLDLLRESVEILRASSYATELARTLVVLGRRLPGGPEAEAVLREAA
GIAAACGVPWLAERAELGLGSAIVPPVATLTPSERRVASLVSRGLTNQAIATELGVSSRA
VEKHLTSAYRKLGVSGRRELVNALPGR

>LuxR family transcriptional regulator [NysRIII]
ATGCTTCTTGAGCGGGAGAACGAACTGGCCCGGATCCGGGCCGCCCTGGACGCCGCGGAA
GCGGGCGACTCCTCGCTCCTCCTGATCAACGGTCCCCTCGGCAGCGGGCGTTCGGCGCTG
CTGCGCCGGATACCGGAGCTGGCCGGCGACGGCACCCGCGTCCTGCGGGCCAGCGCCGCC
TGGCGGGAACGCGACTTCCCCTTCGGGATCGCCCGCCAACTCTTCGACCACCTGCTGTCC
GGGGCGGGCGGCGCAGGGCCGGCCGAACGCACCGCCGGGGCAGAGCACTTCAGCCGACTG
ATGGACACCGGCGACCGCCCTACCGGGACCGGCCCCGCCCTGGAGGTCTCCCAGGCAGTG
CTCCAGGGCGCCCAGGCGCTGCTCGCCGACGCGTCCGCGGAGCGGCGCCTGCTGATCCTG
GTCGACGACCTCCAGTGGGCCGACGGCCCGTCCCTGCGCTGGCTGGCCCACCTCACCCGG
CGGCTGCACGGCCTGCGGGCGCTGCTGGTGTGCACGCTGGCCGACGGCGACCACCGGGGC
AGGTACCCCCTGGTCCGGGAGGTCGCCGGCGCCGCGCACACCGTCCTGCGCCTGGCGCCG
CTGTCCCGGGACGCCACCCGCGTCCTGCTCGCCGGGCCCCAGGGCCGGCCGCCGCAGGAC
GCACTGGTGCGCGCCGTGTACGAGGCGTCCAGGGGCAACCCGCTGTTCCTGACCGCCTTC
CGGAGCGCTCTGCGCGCCACCGGAAGGCCGCCCGGCGGCGACCACTTCGGCGCCGTCCGG
GAGCTGAGCCCGACGGTGCTGCGCGATCGGCTCGCGGGCCATCTGCGGATCCAGCCGCAG
CCGGTGCGCGAGGTCGCGGTGGCGGTGGCCGCGCTGGGCGACCACAGCGATCCGGTGCTG
CTCGCCCAGCTCGCCGGGGTCGATGAAATCGGTTTCGCCGGTGCCCGCCGCGCGCTGGTG
GACGCCGGCCTGTTGGCCCGGGGACGGGACGTCCGCTTCGTCCACGGCGTCGTCCGCGAT
GCGGTGGACTCCCTGCTCACCCTCGACGAGCGGGAACGCTCGCACGACGACGCCGCCGAT
CTGCTGTACCGCTGCGGGCGGCCGGCCGAGCAGGTCGCCGGCCATCTGCTGGCCGTGGTC
CACCCGGGCCGGCCCTGGTCGGAGGCGGTCCTGCGCTCCGCGGCCCACAACGCGCTGCGC
GCCGGCCGGCCCGCCGACGCGGCCCGGTACCTGCGCCGCGCCCTGCTGCACCACCGCACC
CAGGACGGCTGCCGCGCCCGCATCCTGGTCGATCTGGCCACCGCCGAGCGCGCCCTCGAC
CCCGATGCCTGTGTACGCCACGTCAGCCAGGCGGTCGCGCTGCTGGACACCTCGCGGGAT
CGGGCCGCCGCCGTGTTGCGCATCCCGCCGTCCCTGCTCGCCGCCCCCAGCCCGTCCGCC
GTCGAGTTGGTGCGGCAGGCCGCCGCCGGGCTCGACGAACCGGGGCAGCGGGACGAGGAG
GGAGCCGACGAACTCGCCCTGCGCCTGGAGGCGTGGCTGCGGCACTCCGGCCACGAGAAC
CCCGTCGAGCTGGCGTCCTCGGTGGCGCGGCTGCGGCGCATGGGGGCACGGCCGCCGGTG
GACAGCGTCGCCGAACGCGAGTTGGTCGCCGTGCTGTTGAGTGCGGGCGCGCTCAGCGGC
CGGCTCAGCGCCGCGGAGATCGCCGACACCGGCAACCGCATCCTCGAACGTGAGCCGGCC
ACCGCCGCCCATGCCCACACCCCGCTGCCGCTGGTGATGCTCTCGCTGTTCGTCGCCGAG
TCCGTGCAGGGCGTGGCCTCCTGGCTGGCCAGCGAACAGCACACCCGGCGCCGGTACGCG
ACCGGCGCGGACGATGTGCTGCTGACCGCCGAGCGGGCCTTCGTCCTGGTGACGCAGGGC
CGCCCGGCCGCCGCGCGGGAGCACGTCGAACGCGCCCTGGTCATGGACGCCGGCGACTGG
TCGGAACCCGCCGTCATGATGTTCGCCGCGGTCGCCTTCGAACTGCGCGACCCGGCCTTG
AGCGAACGCATCCTGGAACGGATCCGCGACCGCCGGCCGGCCGGACTGGCGCTCACCGCC
ACCGGTCAGATGCTCCAGGCCGCCGTCGACGTGCACTTCGGCCGGGGGCGGGACGCCCTG
GACACGCTGCTGGCCTGTGGCCGACGCCTGGAGACCGTGGGATGGCGCAATTCCGCACTG
CTGCCCTGGCGTCCGTATGCAATCGGGCTGCACCAGCGGCTCGGCGAGACCGATGCCGCG
CTGCAACTCGCCGAGGACGAGCTGAGGTGGGCCCGGGAGTGGGGTGCGACGACGAACCTG
GGCCGGGCCCTGCGCCTGAAGGGCTGGCTGTTGCAGGACGAGGGACTGGATCTGCTGCGT
GAGAGCGTCGAGATCCTCCGCGCTTCGTCCTACGCGACGGAACTGGCGCGCACCCTCGTC
GTCCTCGGGCGTCGGCTGCCGGGTGGGCCGGAGGCCGAGGCGGTGTTGCGGGAGGCCGCG
GGGATCGCCGCCGCCTGTGGTGTTCCCTGGCTCGCCGAACGGGCCGAACTCGGTCTGGGC
AGTGCCATCGTGCCGCCGGTCGCCACCCTGACCCCCAGCGAGCGGCGAGTGGCGTCGCTG
GTGAGCCGGGGCCTGACCAACCAGGCCATCGCGACCGAACTCGGTGTGAGCTCCCGGGCG
GTGGAGAAGCACCTCACCAGCGCCTATCGCAAGCTCGGCGTCTCCGGCCGCCGCGAGTTG
GTGAATGCGCTCCCGGGTCGTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) PR00038 HTHLUXR SM00421 HTH_LUXR PS50043 HTH_LUXR_2 PF00196 GerE IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

Nysta_00180 Nysta_00200