Oligo_00030 Oligo_00030   Oligo_00050 Oligo_00050

Oligo_00040 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameOligomycin
Contig
Start / Stop / Direction3,213 / 3,971 / + [in whole cluster]
3,213 / 3,971 / + [in contig]
Location3213..3971 [in whole cluster]
3213..3971 [in contig]
TypeCDS
Length759 bp (252 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative type II thioesterase
Product (GenBank)thioesterase
Gene
Gene (GenBank)olmC
EC number
Keyword
  • type II thioesterase
Note
Note (GenBank)
Reference
ACC
PmId
[11572948] Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. (Proc Natl Acad Sci U S A. , 2001)
comment
S. avermitilisのゲノムの99%をカバーしたシークエンス解析の報告。
そこで見つかった二次代謝に関するclusterについて述べている。

このORFについての記載は特にされていない。
figにそれらしきORFはあるがコメントなし。
Related Reference
ACC
Q7BUF9
NITE
Rifam_00540
PmId
[19103602] Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. (J Biol Chem. , 2009)
comment
26th, 56%, 6e-71
Amycolatopsis mediterranei_rifR
[Rifam_00540]type II thioesterase

RifRは、acyl-CoAsよりacyl carrier domainのphosphopantetheinyl armからのacyl unitsをhydrolysisをする(Steady-state kinetics)。
helical lidの運動が、RifR active siteへの基質のアクセスを調節する(立体構造解析)。
RifRはCoA基質よりACP基質への反応を好む。これはtype II thioesteraseがcarrier domainsから異常unitsを除くようなhousekeeping functionsをすると考えられていることに一致する。

close this sectionSequence

selected fasta
>putative type II thioesterase [thioesterase]
MSANRVDGNLWVRQFHPAPTAATKLVCLPHAGGSASYFLPVSKALAPGVDVLAIQYPGRQ
DRRHEKCIDNIAELADAVTAVLEPLADRPLVLFGHSMGATLAFEVALRLERKGIVPLALF
ASGRRAPSRHRDESVHLRGDDEIIAELKTLSGTRSEVLGDEEILRMVLPAIRSDYKAAET
YRYAGGARLTAPIHAHFGVEDPRVSLDEAQAWADHTTGHFELRSHPGGHFYLNDQAPRVI
EEIARVSSSVAS
selected fasta
>putative type II thioesterase [thioesterase]
GTGAGTGCCAACCGCGTTGACGGGAACCTGTGGGTCCGCCAGTTCCACCCGGCGCCGACC
GCGGCCACAAAGCTCGTCTGCCTCCCACACGCTGGTGGATCCGCCTCGTACTTCCTGCCC
GTTTCGAAGGCGCTGGCTCCCGGCGTCGACGTACTCGCCATCCAGTACCCGGGGCGGCAG
GACCGGCGCCACGAGAAGTGCATCGACAACATCGCCGAGCTGGCCGACGCCGTGACGGCC
GTACTGGAGCCCCTGGCCGACCGGCCGCTGGTGCTGTTCGGCCACAGCATGGGGGCCACC
CTCGCGTTCGAGGTGGCCCTGCGGCTGGAGCGGAAGGGCATCGTGCCCCTCGCGCTGTTC
GCCTCGGGCCGGCGGGCTCCTTCGCGCCACCGTGACGAGAGCGTGCATCTGCGCGGCGAC
GACGAGATCATCGCCGAGCTGAAGACGCTGAGCGGGACGCGGTCCGAGGTGCTCGGGGAC
GAGGAGATCCTGCGCATGGTGCTGCCCGCCATCCGCAGCGACTACAAGGCGGCCGAGACC
TATCGGTACGCCGGCGGGGCGCGGCTCACGGCGCCGATCCACGCGCATTTCGGGGTGGAG
GATCCGCGGGTCAGCCTCGATGAGGCGCAGGCCTGGGCGGACCACACGACCGGTCACTTC
GAGCTCCGGAGCCATCCGGGCGGCCACTTCTACCTCAATGACCAGGCTCCACGCGTCATC
GAGGAGATCGCCCGGGTCAGTTCGTCCGTCGCTTCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[24-244] 3.09999999999998e-56 PF00975
PF00975   Thioesterase
SignalP No significant hit
TMHMM No significant hit
Oligo_00030 Oligo_00030   Oligo_00050 Oligo_00050
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