Oligo_00040 Oligo_00040   Oligo_00060 Oligo_00060

Oligo_00050 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameOligomycin
Contig
Start / Stop / Direction4,643 / 7,438 / + [in whole cluster]
4,643 / 7,438 / + [in contig]
Location4643..7438 [in whole cluster]
4643..7438 [in contig]
TypeCDS
Length2,796 bp (931 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator
Product (GenBank)luxR-family transcriptional regulatory protein
Gene
Gene (GenBank)olmRI
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11572948] Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. (Proc Natl Acad Sci U S A. , 2001)
comment
S. avermitilisのゲノムの99%をカバーしたシークエンス解析の報告。
そこで見つかった二次代謝に関するclusterについて述べている。

このORFについての記載は特にされていない。
figにそれらしきORFはあるがコメントなし。
Related Reference
ACC
Q9S0N8
NITE
Aver_00010
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[19148632] Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2009)
[20012992] The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. (Mol Genet Genomics. , 2010)
comment
8th, id38%, 1e-122
Streptomyces avermitilis_aveR
LuxR-family transcriptional regulator
avermectinにはpositiveに、oligomycinにはnegativeに作用するregulator.

--
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。
AveR: positive regulator

aveR mutantsの表現型、DNA segmentsを導入することによるその相補特性、helix-turn-helix motifを持つタンパクへの配列類似から、AveRがpotential regulatory functionを持つことが示される。

---
[PMID: 19148632](2009)abstract
aveR 不活化mutantは、avermectin中間体をいかなるavermectin派生体にも変換できない。よってAveRは、avermectinの生合成遺伝子と修飾遺伝子の両方の発現を調節するpositive regulatorであることが示唆された。
また、多量のaveRはavermectinの完全な損失をもたらすことから、aveRには最高許容限界濃度がある。

---
[PMID: 20012992](2010)abstract
aveR deletion mutantはavermectin産生を消失し、oligomycin産生が増える。この表現型はaveR geneのsingle copyによって相補された。

AveRのC末HTH domainの除去はavermectin生合成を消失。
ave clusterとolm clusterの両方のpredicted promoter regionsがAveRのtarget sitesであり、AveRのDNA-binding activityはHTH domainに依存的である。
aveの構造遺伝子の転写はAveRに依存的であるが、olmの構造遺伝子とputative pathway-specific regulatory genesの転写はaveR mutantsで増える。

avermectin PKS_AVES1をコードするaveA1の転写がAveRによって活性化される。
aveRの過剰発現でavermectin産生増加。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator [luxR-family transcriptional regulatory protein]
MLIGRGQEIDRLRAAYSSVRSTGRSRAVLVESAAGCGKSEILEDLVRHVESLGLIALRAT
AIAEERNTPLSVLRSLVNGAPFPDDLVRRFHTMSDAAARMISTEGRDKARRDAAWYELMR
TFCTEVRALAQRAPLVVAVDDIHHGDADSLRQLLHLARHCRNVPVLLTFTQSLAGGPADR
TFGAELLRQPNLEHIRLPRFCRQDIADMLAEQGRADAGQGTVERYFAVSGGNPLLLRALL
EDARLEPDDTVDAQDVGPRAGQAYGRAVLACLDRGGEKAHEVAAGLAVLGGAFTHELLSR
LLDISAAALTENLRALRAAGIVVGTSFQHPAAKAAVLDSLEPSHRLRLHQRSAALLHASG
APAALAAEHLHEAGQADGAWAVGVLQQAAEQALLADEARRAVSYLELAHAASADPRGRTE
IKIRLGSVTRRVSAADAERHVDDALRGFGAGLLTPSDTGGLAALLLAHGRLEQARSVLEQ
GRRPGDGDADRTAARRQLAEIRDSVLNRGAWCAGTRAAWHENPAGRPSTGRAGTGLRAWL
SGSHAALLAQRIAGTEGLLEITHLTDATFDLVLNAVWTLLALGAVERALHWCDTFLREAK
RRDAPGWEAVFAAVRAEGALRGGNLAEAERDARRCFAVLPERHRSALEGSAVSYLVTVQI
ARGKYEDAARQLSRPMADSLPDTIHWLDYLRARGLYYMATRQYHSALRDFHEVGRLAERW
GTDWPALLPWRTDTAEALLRLGEREQGLRLATEQLAMVQDGAPRIKGISLRLQGLASEPA
ARLGLLNRAVVELSRADDRLELSRTLFDLADAHRETRQTRQAAMVLRRARQLAKSCGAQP
PRDRIGSPDAAGASGELFGEGPERNADWGHLSESERRVAGLAASGYTNREISVRLYITMS
TVEQHLTRVYRKLKINGREELLAEARAGVQV
selected fasta
>putative LuxR family transcriptional regulator [luxR-family transcriptional regulatory protein]
GTGCTGATTGGGCGAGGTCAAGAAATAGATCGACTGCGCGCGGCCTATTCTTCAGTTCGT
TCCACCGGTAGATCACGGGCCGTACTCGTCGAGTCGGCGGCCGGGTGCGGCAAGAGCGAG
ATCCTGGAGGATCTCGTACGGCATGTGGAATCGCTCGGCCTGATCGCTCTGCGCGCCACG
GCGATCGCCGAGGAACGAAACACCCCGCTGAGTGTGCTCCGGAGTCTGGTCAACGGGGCT
CCTTTCCCGGACGACCTCGTGCGGCGCTTTCACACGATGAGCGACGCGGCGGCCCGGATG
ATTTCCACGGAGGGCCGGGACAAGGCACGCCGCGACGCCGCCTGGTACGAACTGATGCGG
ACCTTCTGTACCGAGGTCCGCGCCCTCGCCCAGCGGGCGCCCCTGGTGGTGGCCGTCGAC
GACATCCACCACGGGGACGCCGACTCCCTTCGCCAGCTGCTCCATCTGGCCCGGCACTGC
CGCAACGTGCCGGTGCTGCTGACGTTCACGCAGTCGCTCGCCGGCGGTCCGGCGGACCGG
ACCTTCGGCGCGGAACTGCTGAGGCAGCCGAACCTCGAACACATACGGCTGCCGAGGTTC
TGTCGTCAGGACATCGCCGACATGCTGGCCGAGCAGGGACGCGCGGACGCCGGTCAGGGC
ACGGTGGAGCGCTACTTCGCGGTCAGCGGCGGCAATCCGCTGCTGCTGCGAGCCCTCCTG
GAGGACGCCCGCCTCGAGCCCGACGACACCGTCGACGCCCAGGACGTGGGGCCCCGGGCC
GGGCAGGCGTACGGCCGCGCGGTCCTCGCCTGTCTGGACCGCGGCGGAGAGAAGGCACAC
GAGGTCGCCGCCGGACTCGCCGTGCTGGGCGGGGCGTTCACCCATGAACTGCTCTCCCGC
TTACTCGACATCTCCGCCGCGGCGCTCACCGAGAACCTGCGCGCGCTGCGGGCCGCCGGC
ATCGTGGTCGGCACGTCCTTCCAGCACCCGGCGGCCAAGGCCGCGGTCCTGGACTCCCTG
GAGCCGTCGCACCGCCTCCGGCTGCACCAGCGGTCCGCGGCGTTGCTGCACGCCTCCGGC
GCACCGGCGGCCCTGGCGGCCGAACACCTCCACGAGGCCGGACAGGCCGACGGCGCCTGG
GCGGTGGGCGTGCTCCAGCAGGCCGCCGAACAGGCCCTGCTGGCGGACGAGGCACGGCGG
GCCGTGTCGTACCTCGAACTCGCCCACGCCGCGAGCGCGGACCCGCGGGGCCGTACCGAG
ATCAAGATCAGGCTCGGCTCCGTCACGCGCCGGGTCAGCGCGGCCGACGCCGAACGCCAT
GTGGACGACGCGCTGCGGGGGTTCGGGGCCGGCCTGCTCACCCCGTCGGACACGGGCGGC
CTCGCCGCGCTGCTGCTGGCCCACGGACGGCTGGAACAGGCGCGCTCGGTGCTGGAGCAG
GGACGGCGGCCGGGCGACGGCGACGCCGACCGCACAGCCGCGCGGCGACAGCTCGCCGAG
ATCCGCGACAGCGTGCTGAACCGGGGCGCGTGGTGCGCGGGCACCCGGGCCGCCTGGCAC
GAGAACCCCGCCGGGCGGCCGTCGACGGGCCGGGCGGGCACCGGCCTGCGCGCCTGGCTC
AGTGGGTCGCACGCCGCGCTGCTGGCCCAGCGCATCGCGGGCACCGAGGGCCTGCTGGAG
ATCACACACCTGACCGACGCCACGTTCGACCTGGTACTCAACGCGGTCTGGACGCTGCTC
GCGCTCGGCGCCGTCGAGCGGGCCCTGCACTGGTGCGACACGTTCCTGCGAGAGGCGAAG
CGGCGCGACGCGCCGGGCTGGGAGGCGGTCTTCGCGGCCGTACGCGCCGAGGGGGCCCTG
CGCGGCGGCAATCTGGCCGAGGCGGAGCGGGACGCCCGCCGCTGTTTCGCGGTCCTGCCG
GAACGGCACCGGTCGGCCCTGGAGGGCAGCGCGGTCTCGTACCTGGTGACGGTCCAGATC
GCGCGGGGCAAGTACGAGGACGCGGCACGGCAGTTGAGCCGGCCCATGGCGGACTCGCTG
CCCGACACCATCCACTGGCTCGACTATCTGCGGGCCCGCGGGCTCTACTACATGGCCACC
CGGCAGTACCACTCGGCGCTGCGTGACTTCCACGAGGTGGGCCGCCTCGCCGAGCGGTGG
GGCACGGACTGGCCCGCCCTGCTGCCCTGGCGCACGGACACGGCCGAGGCGCTGCTGCGC
CTCGGGGAGCGGGAGCAGGGGCTGCGGCTGGCCACCGAGCAGCTGGCCATGGTGCAGGAC
GGCGCCCCGCGCATCAAGGGCATCTCACTGCGCCTCCAGGGGCTCGCGTCGGAGCCCGCC
GCCCGGCTCGGGCTGCTCAACCGGGCCGTCGTGGAGCTGAGCCGCGCGGACGACCGGCTG
GAGCTCTCGCGCACCCTGTTCGACCTGGCCGACGCCCACCGGGAGACCCGGCAGACCCGG
CAGGCGGCGATGGTCCTGCGCCGGGCGCGGCAACTGGCGAAGAGCTGCGGGGCGCAGCCG
CCCCGGGACCGGATCGGTTCGCCCGACGCCGCGGGGGCGTCGGGGGAACTCTTCGGCGAG
GGACCCGAACGGAACGCCGACTGGGGACATCTCAGCGAGTCCGAACGCCGGGTCGCCGGC
CTGGCCGCCAGCGGATATACGAACCGTGAGATCTCGGTAAGACTCTACATAACCATGAGT
ACGGTGGAGCAACACCTTACCCGAGTTTACCGAAAGCTAAAGATAAACGGACGTGAGGAA
TTGCTGGCCGAAGCCCGCGCGGGTGTGCAGGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[871-885] 1.9000006847787e-08 PR00038 [885-901] 1.9000006847787e-08 PR00038 [901-913] 1.9000006847787e-08 PR00038
PR00038   HTHLUXR
[864-929] PS50043
PS50043   HTH_LUXR_2
[868-925] 2.49999811956465e-21 SM00421
SM00421   HTH_LUXR
[885-912] PS00622
PS00622   HTH_LUXR_1
[871-924] 1.7e-11 PF00196
PF00196   GerE
IPR011990 Tetratricopeptide-like helical (Domain)
[577-756] 1.8e-14 G3DSA:1.25.40.10 [792-879] 1.8e-14 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[880-926] 3.5e-17 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[849-927] 3.00000067992871e-14 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Oligo_00040 Oligo_00040   Oligo_00060 Oligo_00060
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