Oligo_00120 Oligo_00120   Oligo_00140 Oligo_00140

Oligo_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameOligomycin
Contig
Start / Stop / Direction83,059 / 81,845 / - [in whole cluster]
83,059 / 81,845 / - [in contig]
Locationcomplement(81845..83059) [in whole cluster]
complement(81845..83059) [in contig]
TypeCDS
Length1,215 bp (404 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)cytochrome P450
Gene
Gene (GenBank)olmB
EC number
Keyword
  • C-12 hydroxylate
Note
Note (GenBank)
Reference
ACC
PmId
[11572948] Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. (Proc Natl Acad Sci U S A. , 2001)
[25849761] Functional characterization of CYP107W1 from Streptomyces avermitilis and biosynthesis of macrolide oligomycin A. (Arch Biochem Biophys. , 2015)
[26883908] Structural Analysis of the Streptomyces avermitilis CYP107W1-Oligomycin A Complex and Role of the Tryptophan 178 Residue. (Mol Cells. , 2016)
comment
[PMID: 11572948](2001)
S. avermitilisのゲノムの99%をカバーしたシークエンス解析の報告。
そこで見つかった二次代謝に関するclusterについて述べている。

このORFについての記載は特にされていない。
PKS genesの間にある。

---
[PMID: 25849761](2015) 本文未確認
CYP107W1の生化学的機能調査と構造解析。
CYP107W1はoligomycin CのC12を位置選択的にhydroxylateしてoligomycin Aを産生する。

この論文はQ93HJ0に登録されており、CYP107W1はolmBに相当する模様。
Related Reference
ACC
Q59523
PmId
[7808395] Characterization and expression of a P-450-like mycinamicin biosynthesis gene using a novel Micromonospora-Escherichia coli shuttle cosmid vector. (Mol Gen Genet. , 1994)
comment
BLAST id43%
Micromonospora griseorubida_mycG
P-450-like protein

abstract
塩基配列と変異の解析から、single P-450-like proteinがhydroxylation and epoxidation 両方の反応を触媒すると示唆される。
ACC
Q54302
NITE
Rapam_00170
PmId
[11372782] Deletion of rapQONML from the rapamycin gene cluster of Streptomyces hygroscopicus gives production of the 16-O-desmethyl-27-desmethoxy analog. (J Antibiot (Tokyo). , 2001)
[16990929] Rapamycin biosynthesis: Elucidation of gene product function. (Org Biomol Chem. , 2006)
comment
BLAST id41%
Streptomyces hygroscopicus_rapN
Cytochrome P450

--
[PMID: 11372782](2001)
rapQONMLのdeletion mutantはpipecolateを添加すると、rapamycinの代わりにanalog 16-O-desmethyl-27-desmethoxyrapamycinを産生した。これは、rapNによってコードされるP450 hydroxylaseがC-27に特異的で、rapQとrapM によってコードされるO-methyltransferasesがC-16 and C-27でのhydroxyl基をメチル化することを示す。

--
[PMID: 16990929](2006)
rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10の相補実験で、rapNを含んだ株では特異的にC-27がhydroxylationされた産物が得られる。

close this sectionSequence

selected fasta
>cytochrome P450 [cytochrome P450]
MAEAPSEPIAFPFPDPPSVCELPPELAEIRDGQSVVEVKFPDGISGWMVTKHADVRKVLV
DSRFSSKVMATAAAAMSETETGKLMNESLVGMDAPEHTRLRKLVTKAFTARRVETLRPRI
TELVGQLLDELETLPRPVDLVKNFSVPLPVRVICELLGVPAGDQDTFHAWSNALLGDWQQ
VVEKEAATVSLVNYFGELIAVKRENPADDLISELIAISDGDSTLTEREIIALSIGILSAG
HETTANQISMFLVTLLHNPEELDKLRDNREAIPKAVDELLRFVPLTTTGGIIPRLTTAEV
ELSGGQVLPAGAVVLPAVATANRDPEVFEDGERLNVTRENNPHLAFGAGIHHCLGAQLAR
IELQEALGAILDRMPQVRLAVPESELRLKSASIIRGLESLPITW
selected fasta
>cytochrome P450 [cytochrome P450]
ATGGCCGAAGCGCCTTCCGAGCCCATCGCGTTCCCGTTCCCCGACCCGCCGTCGGTCTGC
GAGCTGCCTCCCGAGCTGGCGGAGATCCGCGACGGCCAGTCCGTGGTGGAGGTGAAGTTC
CCCGACGGCATCAGCGGATGGATGGTGACCAAGCACGCCGATGTCCGCAAGGTGCTGGTC
GACTCCCGGTTCAGCAGCAAGGTGATGGCCACCGCGGCCGCCGCGATGTCGGAGACCGAG
ACCGGCAAGCTGATGAACGAGTCGCTCGTCGGCATGGACGCGCCGGAGCACACGCGGCTG
CGCAAGCTGGTGACCAAGGCGTTCACCGCGAGGCGGGTGGAGACGCTGCGGCCCCGCATC
ACGGAGTTGGTCGGCCAGCTGCTCGACGAGCTGGAGACGCTGCCCCGCCCGGTGGACCTG
GTCAAGAACTTCTCCGTGCCGCTGCCGGTCCGGGTGATCTGCGAACTCCTCGGCGTACCG
GCCGGGGACCAGGACACCTTCCACGCCTGGTCGAACGCGCTGCTCGGGGACTGGCAGCAG
GTGGTGGAGAAGGAGGCCGCGACGGTCTCGTTGGTGAACTACTTCGGCGAACTCATCGCG
GTGAAGCGGGAGAATCCCGCCGACGACCTGATCAGTGAGCTGATCGCGATCAGCGACGGG
GACAGCACGCTCACCGAGCGCGAGATCATCGCGCTGAGCATCGGCATTCTCAGCGCGGGC
CACGAGACGACCGCCAACCAGATCAGCATGTTCCTGGTGACGCTGCTGCACAATCCCGAG
GAGCTCGACAAGCTGCGGGACAACCGCGAGGCGATCCCGAAGGCCGTCGACGAGCTGCTC
CGCTTCGTTCCGCTCACCACGACCGGCGGCATCATTCCCCGGCTCACCACGGCGGAGGTG
GAGCTGAGCGGCGGCCAGGTGCTGCCCGCCGGCGCGGTGGTCCTGCCCGCGGTCGCCACC
GCGAACCGGGACCCCGAGGTGTTCGAGGACGGCGAGCGGCTGAACGTGACGCGGGAGAAC
AATCCGCATCTGGCCTTCGGCGCCGGTATCCACCACTGCCTCGGGGCGCAGCTCGCCCGC
ATCGAGCTCCAGGAGGCGCTCGGCGCGATCCTGGACCGGATGCCCCAGGTGCGGCTCGCG
GTGCCGGAGTCGGAGCTGCGGCTCAAGTCCGCGTCGATCATCCGCGGCCTCGAGAGCCTG
CCCATCACCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[239-256] 7.70000171387738e-09 PR00385 [274-285] 7.70000171387738e-09 PR00385 [344-353] 7.70000171387738e-09 PR00385 [353-364] 7.70000171387738e-09 PR00385
PR00385   P450
[5-404] 3.89999861795218e-98 SSF48264
SSF48264   Cytochrome_P450
[204-373] 5.59999999999999e-24 PF00067
PF00067   p450
[15-404] 4.2e-108 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
[92-103] 1.10000150671643e-45 PR00359 [139-155] 1.10000150671643e-45 PR00359 [156-171] 1.10000150671643e-45 PR00359 [193-215] 1.10000150671643e-45 PR00359 [274-285] 1.10000150671643e-45 PR00359 [323-338] 1.10000150671643e-45 PR00359 [344-353] 1.10000150671643e-45 PR00359 [353-364] 1.10000150671643e-45 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[346-355] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Oligo_00120 Oligo_00120   Oligo_00140 Oligo_00140
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