Phosl_00060 Phosl_00060   Phosl_00080 Phosl_00080

Phosl_00070 : CDS information

close this sectionLocation

Organism
StrainHK803
Entry namePhoslactomycin
Contig
Start / Stop / Direction15,006 / 20,585 / + [in whole cluster]
15,006 / 20,585 / + [in contig]
Location15006..20585 [in whole cluster]
15006..20585 [in contig]
TypeCDS
Length5,580 bp (1,859 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)Plm5
Gene
Gene (GenBank)plm5
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase type I
Reference
ACC
PmId
[12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003)
comment
Phoslactomycins生合成gene clusterのクローニング、配列解析報告。

plm5はPKSのModule 5(KS-AT-DH-KR-ACP)をコードする。

close this sectionPKS/NRPS Module

5 malonyl-CoA
KS34..410
AT565..886
DH931..1095
KR1423..1603
ACP1710..1780

close this sectionSequence

selected fasta
>polyketide synthase [Plm5]
MRSEEQLLDYLKRVTADLHDTRVRFQAAEEAAREPVAIVGMGCRYPGGVFSPEDLWQLVA
EGRDVIGDLPTDRSWNLDTLYDPDPTAPGTTYARGGGFVDTATHFDAGFFGISPREAAAM
DPQQRLVLETGWEALERAGIPPASLGGSDTGVYIGTGMQDHIIHLQKSRQEAEGFVGTGN
AISVVSGRLAYTLGLEGPAVSVDTACSSSLVALHLAVQALRAGECSLALAGGATVINTPV
MFVEFSRQRGLSPDGRCRSFAADADGTGWSEGVGVLVLERLSDARRHGHQVLAVVRGSAV
NQDGASNGLTAPNGPSQQRVIRRALTAARLTADEVDAVEAHGTGTRLGDPIEAQALLATY
GQGRPADRPLWLGSLKSNIGHAQAAAGVGGVIKTVMALRHGVLPQTLHVDAPTPEVDWSA
GEVRLLTQRREWPRAGRPRRAGVSAFGISGTNAHLILEEAPEEAPEDAADRAPHLRGMPW
VVSGRGRQALRAQAARLRDWAAGHPDVAPEKVGLALATRRSVFEHTAVITGADHDELLSG
LSALSEGRPAPGVRLETAAGGGLAFAFTGQGAQRPGMGRGLYETFPAYAEAFDEACAALD
PHLERPLASVVFADDPAAAEALKTTAYAQPALFAVETALFRLMQSIGVHPDLLIGHSVGE
LSAAHAAGVLSLQDAARLVAARGRLMQSLPEDGRMLAIQASEDEVLPLVAELSEKAGGLA
LAAVNGPESVVVSGRTEAVHELEKEFAGRGRRVRYLDVSHAFHSPLMDPVLDEFARIAAS
VTLRPATIPVVSNVTGDLAGDDRLADPAYWADHIRATVRFADGVRALAREQVDTVVELGP
DAALTALGGEILDQDTAAFVPTLSRKHDETSTFLTALARLHARGIPVRWPAATTPVESPD
LPTYAFQRERHWLDGVAETDVAGTGLTGIGHPLLPAETALPGTDGVVLTGSLSLRDHAWL
ADHAVLGVVLVPGAGLLDMALTAAEHAGCTQVEELTLESPLILPEQGARSVQVLVGAAQD
SGQRAITIHSRLQDGDPHAAWTRHATGLLATGPQEEPATSSEAWPPTGAVPVPVDDLYLR
LTEGGVDYGPSFRGLRAAWRLGEDFYADIDLPLQSDAERFMLHPALLDAALHSLALPGAI
LHTGQAHLPFSWSGVRLHASGADALRVRVRGTGPSSVSLELADGTGRPVATVGELALRPV
SQEQLRTPGADATSLYTVEWPVKELPEGADGSAARTWALIAAAESDGYEVKGVELSHCTS
LPALAAALDATDAPVPEAVLVPCFLPHSPGCPPDDSADLVERAHDYAATVLDLVRTWLGD
ARFEASRLLLLTRGAVTPDAGHHPLSDAVGSLAWGLVRTVQNEHPGRVLVADLDDDPASW
SVLPGVLAHEEPQVAVRRGVAHVPRLTPARAAAERPAPFDSMGTVLVTGGTGGLGSLLAR
HLVVEHGVRHLLLTSRRGPQAPGAAELAAELIELGAQVTVAACDMADSDAVEELIGSLPA
GHPLTAVIHTAGVLDDGLVEDLTRERLNTVLRPKADAAVVLDRLTRHLDLTAFVLYSSVG
GTFGGPGQGNYAAANAFLDAVAHRRRAEGLPGLSLGWGLWSDTTGMAAEIGTTHVDRLNR
SGLVTMSPAEGLALFDAAISGEYGPVVLPVRLDLPALKARATSGALPSVLADVVRTPAGP
ARAAKSGTVSGAALRTSLSELPEDERRRMLLDVVRENVAAVLGHHQDGTVDDEQPFKDLG
LDSLTAVELRNRLASATGLTLPATLLFDLPSPSALAHHLLTRMGVQESKSPVAEAVDHLT
ALLTTHDVGDLERGRVTARLRSLLWRLDDGGAESTEDAADQAETDDDIFALADRELGLA
selected fasta
>polyketide synthase [Plm5]
ATGCGATCCGAAGAACAATTGCTGGACTACCTGAAGCGCGTGACCGCGGACCTGCACGAC
ACCCGGGTGCGGTTTCAGGCGGCCGAGGAGGCTGCCCGCGAGCCGGTCGCGATCGTCGGG
ATGGGGTGCCGATACCCGGGCGGCGTGTTCTCGCCGGAGGATCTGTGGCAGTTGGTCGCC
GAGGGACGCGACGTCATCGGAGATCTGCCCACCGACCGTTCCTGGAACCTTGACACCCTC
TACGACCCCGATCCGACGGCACCCGGTACCACGTACGCCCGCGGCGGTGGGTTCGTCGAC
ACAGCCACCCATTTCGACGCGGGGTTCTTCGGGATTTCACCGCGGGAAGCCGCCGCGATG
GACCCGCAGCAGCGGCTGGTCCTGGAGACGGGCTGGGAGGCGTTGGAGCGCGCCGGAATT
CCCCCGGCGTCGCTCGGCGGGAGCGACACCGGTGTCTATATCGGTACGGGCATGCAGGAC
CACATCATCCACCTCCAGAAGTCCCGGCAGGAGGCCGAGGGATTCGTCGGCACCGGTAAC
GCCATCTCGGTGGTGTCCGGCCGGCTCGCCTACACCCTCGGTCTCGAAGGCCCCGCCGTC
AGCGTGGACACGGCCTGCTCGTCCTCGCTGGTGGCACTGCACCTCGCGGTCCAGGCACTG
CGCGCCGGAGAGTGCTCGCTGGCCCTGGCCGGAGGGGCGACGGTCATCAACACCCCTGTG
ATGTTCGTGGAGTTCAGCCGCCAGCGCGGACTGTCCCCGGACGGCAGGTGCCGGTCCTTC
GCGGCGGACGCGGACGGCACCGGCTGGTCGGAGGGCGTGGGTGTCCTCGTACTGGAGAGG
CTGTCGGACGCGCGGCGTCACGGGCACCAGGTGCTCGCCGTCGTCCGTGGGTCGGCGGTC
AACCAGGACGGCGCCTCGAACGGTCTGACGGCGCCGAACGGACCGTCGCAGCAACGGGTG
ATCCGGCGGGCACTGACCGCGGCCCGGCTGACGGCCGATGAGGTCGATGCGGTGGAGGCG
CACGGTACGGGGACGCGGCTGGGTGATCCGATCGAGGCGCAGGCGTTGTTGGCGACGTAC
GGCCAGGGCCGGCCCGCCGATCGGCCCTTGTGGCTGGGGTCGTTGAAGTCGAACATCGGA
CATGCACAGGCTGCCGCGGGTGTCGGTGGCGTGATCAAGACGGTGATGGCGTTGCGGCAC
GGGGTGTTGCCGCAGACGCTGCATGTGGATGCGCCGACGCCGGAGGTGGACTGGTCGGCG
GGCGAGGTGCGGTTGCTGACACAGCGGCGGGAGTGGCCGCGTGCGGGCCGGCCGCGCCGG
GCGGGCGTGTCCGCCTTCGGGATCAGCGGCACCAACGCCCACCTCATCCTCGAAGAAGCC
CCAGAAGAGGCTCCCGAAGATGCGGCTGACCGAGCGCCCCACCTCCGGGGCATGCCGTGG
GTGGTGTCCGGGCGGGGCAGGCAGGCACTGCGGGCTCAGGCGGCCCGACTGCGGGACTGG
GCCGCGGGACACCCGGACGTGGCACCGGAGAAGGTAGGCCTGGCCCTGGCCACCCGGCGG
TCGGTGTTCGAGCACACTGCGGTGATCACCGGCGCCGACCACGACGAACTGCTGAGCGGG
CTGTCGGCCCTGAGCGAGGGCCGGCCGGCGCCGGGCGTACGTCTGGAGACGGCGGCCGGG
GGCGGTCTCGCCTTCGCGTTCACCGGGCAGGGAGCCCAGCGCCCCGGCATGGGACGCGGC
CTGTACGAGACCTTTCCCGCGTACGCCGAGGCGTTCGACGAGGCGTGCGCGGCACTGGAC
CCGCACCTGGAGCGCCCGTTGGCGTCGGTGGTCTTCGCTGACGATCCCGCCGCCGCCGAG
GCGCTGAAGACGACGGCGTACGCACAGCCGGCCCTGTTCGCCGTGGAGACGGCGCTGTTC
CGTCTGATGCAGTCGATCGGCGTCCACCCGGATCTGCTGATCGGGCATTCAGTGGGCGAG
CTGTCCGCTGCGCACGCGGCCGGGGTGCTCTCCCTTCAGGACGCGGCACGGCTGGTGGCG
GCCCGTGGGCGGCTGATGCAATCCCTGCCCGAGGACGGACGGATGCTCGCGATCCAGGCG
TCGGAGGACGAGGTGCTGCCGTTGGTCGCCGAGCTGTCGGAGAAGGCCGGCGGCTTGGCG
CTGGCCGCGGTGAACGGGCCCGAGTCGGTGGTGGTCTCGGGCCGCACCGAGGCCGTGCAC
GAGCTGGAGAAGGAGTTCGCCGGGCGTGGCCGCCGCGTCCGGTACCTCGACGTGAGCCAC
GCGTTCCACTCCCCGCTCATGGATCCGGTTCTGGACGAGTTCGCCCGGATCGCGGCATCG
GTCACCTTGCGGCCGGCGACGATCCCCGTCGTCTCCAACGTGACCGGGGACCTGGCCGGC
GACGACCGGCTCGCGGACCCCGCCTATTGGGCGGACCACATCCGCGCCACGGTCCGGTTC
GCCGACGGAGTACGGGCGTTGGCCCGCGAGCAGGTGGACACCGTGGTCGAACTGGGCCCG
GACGCCGCACTCACGGCGCTCGGCGGCGAGATCCTCGACCAGGACACCGCCGCTTTCGTG
CCCACCCTCAGCCGCAAGCACGACGAGACGTCCACGTTCCTGACGGCGCTGGCCCGGCTG
CACGCCCGTGGCATCCCCGTCCGCTGGCCCGCCGCGACCACACCGGTCGAATCGCCGGAC
CTGCCCACCTACGCCTTCCAGCGGGAGCGCCACTGGCTCGACGGCGTGGCGGAGACCGAT
GTCGCGGGCACCGGCCTGACCGGCATCGGCCATCCGCTCCTGCCGGCGGAGACCGCGCTA
CCGGGCACGGACGGTGTCGTCCTCACCGGCAGCCTGTCGCTGCGGGACCATGCCTGGCTC
GCCGACCACGCCGTGCTGGGCGTGGTGCTGGTGCCCGGCGCGGGGCTGCTGGACATGGCT
CTGACCGCCGCCGAGCACGCGGGCTGCACCCAGGTCGAGGAGCTCACGCTGGAGAGCCCG
CTGATCCTCCCCGAACAGGGAGCCCGTTCCGTGCAGGTCCTGGTCGGCGCGGCCCAGGAC
TCCGGGCAGCGCGCGATCACGATCCACTCGCGGCTGCAGGACGGTGACCCGCACGCTGCT
TGGACGCGCCACGCCACCGGGCTGCTCGCCACAGGCCCGCAGGAGGAGCCGGCCACCTCT
TCGGAAGCCTGGCCGCCGACCGGGGCCGTCCCCGTCCCTGTCGACGACCTCTACCTCCGC
CTGACCGAGGGAGGCGTCGACTACGGACCGTCGTTTCGGGGACTGCGGGCCGCATGGCGG
CTCGGCGAGGACTTCTACGCCGACATCGATCTTCCCCTCCAGTCCGACGCGGAGCGGTTC
ATGCTGCACCCCGCGCTGCTGGACGCCGCCCTGCATTCCCTTGCCCTGCCCGGCGCGATC
CTGCACACGGGCCAGGCACACCTGCCGTTCTCCTGGAGCGGGGTACGGCTGCACGCCTCG
GGCGCCGACGCGCTGCGCGTACGGGTCCGGGGCACGGGACCGAGCAGCGTCAGCCTGGAG
CTGGCCGACGGTACGGGCCGGCCCGTGGCGACCGTCGGTGAACTCGCCCTCCGTCCGGTG
TCCCAGGAGCAGTTGCGGACCCCCGGCGCCGACGCCACCTCTCTGTACACGGTGGAGTGG
CCCGTGAAGGAGCTGCCCGAGGGCGCGGACGGCTCCGCGGCGCGGACCTGGGCGCTGATC
GCAGCAGCCGAGTCCGACGGGTACGAGGTCAAGGGCGTCGAGCTGAGCCACTGCACGAGT
CTCCCGGCGCTCGCCGCCGCCCTCGACGCCACGGATGCACCCGTGCCCGAAGCGGTCCTC
GTCCCCTGCTTCCTCCCCCACTCCCCCGGCTGCCCGCCGGACGACTCCGCCGACCTCGTC
GAGCGGGCGCACGACTACGCCGCGACCGTCCTCGACCTCGTGCGCACATGGCTGGGGGAT
GCGCGTTTCGAGGCTTCCCGACTCCTCCTGCTCACCCGAGGCGCGGTCACGCCCGATGCC
GGCCACCATCCCCTCAGCGACGCAGTGGGCAGTCTCGCCTGGGGGCTCGTCCGGACCGTG
CAGAACGAGCATCCCGGGCGGGTCCTGGTGGCAGACCTCGACGACGATCCGGCGTCCTGG
TCCGTGCTGCCCGGTGTACTGGCCCACGAGGAACCCCAGGTGGCCGTGCGCCGTGGTGTC
GCCCACGTGCCCCGCCTCACCCCGGCCCGCGCCGCCGCGGAGCGGCCCGCCCCCTTCGAC
AGCATGGGAACCGTGCTGGTCACCGGCGGTACGGGAGGACTGGGCAGTCTACTGGCCCGT
CATCTCGTGGTCGAGCACGGTGTACGGCATCTGCTGCTGACCAGCCGACGTGGTCCGCAG
GCGCCCGGAGCCGCTGAACTCGCCGCCGAACTGATCGAGTTGGGAGCGCAGGTGACGGTC
GCCGCGTGCGACATGGCCGACTCCGACGCCGTCGAGGAACTCATCGGCTCGCTCCCCGCC
GGCCATCCGCTCACCGCGGTGATCCACACGGCCGGCGTCCTGGACGACGGTCTCGTCGAG
GATCTGACACGGGAACGGCTGAATACGGTACTGCGTCCGAAGGCCGACGCCGCCGTCGTC
CTGGACCGGCTGACACGTCACCTGGATCTGACCGCGTTCGTGCTCTACTCCTCCGTCGGA
GGCACCTTCGGCGGTCCGGGCCAGGGCAACTACGCCGCCGCGAACGCCTTCCTGGACGCG
GTCGCCCATCGCCGCCGCGCCGAGGGACTCCCCGGGCTCTCGCTGGGCTGGGGGCTGTGG
AGCGACACCACCGGCATGGCCGCCGAGATCGGCACCACGCACGTCGACCGCCTGAACCGC
TCCGGACTGGTGACGATGTCCCCCGCCGAGGGGCTGGCGTTGTTCGACGCGGCGATCAGC
GGGGAATACGGCCCTGTGGTCCTGCCCGTCCGTCTCGACCTGCCGGCGCTCAAGGCCCGC
GCCACCTCAGGAGCGTTGCCGTCGGTGCTCGCCGACGTGGTCCGCACACCCGCGGGTCCG
GCCCGCGCGGCGAAGAGCGGCACGGTGTCCGGGGCCGCGCTGCGGACGAGCCTGTCGGAG
CTGCCGGAGGACGAGCGCCGCCGAATGCTGCTGGACGTCGTACGCGAGAACGTGGCAGCC
GTGCTGGGCCACCATCAGGACGGGACGGTCGACGACGAGCAGCCGTTCAAGGACCTCGGG
CTCGACTCCCTCACCGCTGTGGAGCTGCGCAACCGCCTCGCCTCCGCCACCGGTCTCACC
CTCCCGGCGACCCTCCTGTTCGATCTGCCCTCGCCGTCGGCGCTCGCCCACCATCTGCTC
ACCCGCATGGGGGTGCAGGAGAGCAAGTCCCCGGTGGCCGAGGCCGTCGATCACCTCACG
GCGCTGCTCACCACCCATGACGTGGGGGACCTGGAACGCGGCCGGGTCACCGCCCGACTG
CGCTCCCTGCTGTGGCGGCTCGACGACGGCGGGGCGGAGAGTACGGAGGACGCCGCGGAC
CAGGCGGAGACGGACGACGACATCTTCGCGCTCGCCGATCGGGAGCTGGGTCTGGCGTGA
[5] KS34..410
[5] AT565..886
[5] malonyl-CoA760..764
[5] DH931..1095
[5] KR1423..1603
[5] ACP1710..1780
[5] KS100..1230
[5] AT1693..2658
[5] malonyl-CoA2278..2292
[5] DH2791..3285
[5] KR4267..4809
[5] ACP5128..5340

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[558-689] 5.09999999999993e-80 G3DSA:3.40.366.10 [760-876] 5.09999999999993e-80 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1423-1590] 1.5e-62 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
[1738-1753] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1708-1782] 9.7e-23 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1701-1787] 4.80000830876748e-22 SSF47336
SSF47336   ACP_like
[1710-1780] PS50075
PS50075   ACP_DOMAIN
[1713-1779] 7.30000000000002e-14 PF00550
PF00550   PP-binding
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[34-284] 4.30000000000003e-98 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[292-410] 1.29999999999998e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[565-886] 6.40000000000004e-69 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-26] 8.40000000000001e-11 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[562-870] 6.10002656149838e-72 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[691-759] 2.70000183580794e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[36-296] 2.50000000000001e-91 G3DSA:3.40.47.10 [297-463] 2.20000000000002e-65 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[26-408] 1.19999063421924e-104 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1423-1640] 1.80000000000002e-34 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[197-213] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[566-867] 2.59998233910302e-124 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1711-1783] 3.49999466863949e-33 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[931-1095] 7.29994178862692e-79 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[36-462] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1423-1603] 2.90000354677981e-64 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Phosl_00060 Phosl_00060   Phosl_00080 Phosl_00080
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