Phosl_00050 Phosl_00050   Phosl_00070 Phosl_00070

Phosl_00060 : CDS information

close this sectionLocation

Organism
StrainHK803
Entry namePhoslactomycin
Contig
Start / Stop / Direction10,164 / 14,963 / + [in whole cluster]
10,164 / 14,963 / + [in contig]
Location10164..14963 [in whole cluster]
10164..14963 [in contig]
TypeCDS
Length4,800 bp (1,599 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)Plm4
Gene
Gene (GenBank)plm4
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase type I
Reference
ACC
PmId
[12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003)
comment
Phoslactomycins生合成gene clusterのクローニング、配列解析報告。

plm4はPKSのModule 4(KS-AT-KR-ACP)をコードする。
ethylmalonyl-CoA extender unitを使用する。

close this sectionPKS/NRPS Module

4 ethylmalonyl-CoA
not conserved XAGHS
KS4..352
AT510..829
KR1157..1336
ACP1446..1516

close this sectionSequence

selected fasta
>polyketide synthase [Plm4]
MSAARVASGSFPVDRGWDLDALFSEAADGKAASYVREGGFLYDAGWFDASFFGISPREAV
TIDPQQRQLLEVAWEALERARISPQALRGSDVGVFAGAMNQDYAVRLHESIEDFEGFLTT
GNTGSVVSGRLSYTLGLVGPAVTVDTACSSSLVTMHLAARSLRSRECSLALAGGVTVMSM
PTTFTEFCRQRNLAPDGRAKSFAAAADGTAWAEGVGVLVLERLSDAERNGHPVLALIRGS
AVNQDGASNGLSAPNGPSQERVIWQALRDARLTADEVDAVEGHATGTRLGDPIEAQALLA
TYGQGRSADRPLWLGSLKSNIGHAQAAAGVGGVIKTVMALRHGVLPQTLHVDAPTPKVDW
SAGEVRLLTQRREWPRAGRPRRAGVSSFGVSGTNAHLILEEAPQAPPHDAARDRTDTGGG
ATLPLVLSARTAAAVREQARRLHSHLTDHPELVPAQVAGSLVTTRSTFDQRAVVLGTDRA
ELLDGLDSVAQGVPDARTVTGSAVGGREVVFVFPGQGGQWAGMALELMEEFPVFAETLHS
CADALADHVDWSLLDVLRQAEGAPGLDRVDVIQPVLFSVTVALADLWRSLGIEPSAVVGS
SLGEIAAAYTAGTLSLADATKVAVLRSRALLELSGRSGMVSVPLGRVQVEELIAAWPDRL
FVAGVNSPSISVVSGDNEAVDELLAVCAERGVRARRVATDCASHYPAVEALEERLLTDLA
DVAPTPARIAFLSTVSGESAETETVPDAAYWYRNTRRTVEFGPAVQRLAATGSKVYIEIS
PHPVLQVALSEIVEGESREAAVLSTLRRNTSDRRAFLTSLAKAYVSGVTVDWAALPDLAG
APPVDLPTYAFQRERYWPRPAAASNGSRGPGAGAPATVGQGTVDAHFWEAVENGDLGGLG
PDVRFDDETPLKDVLPELASWHRQGLEQARVDGWRYVEKWRPLDVPATGPHGRWLLVHPG
GTESDATTEWVRDGLLEAGCSVDDLLVDTTDTDTAAVTERLKQACAGGGPEPVGVVSLLA
FDGRHDTACPSVPRGTAATLALVRALGAAGITAPLWCLTQGAVTTGTGDRLGAVEQAQIW
GLGRVVALEHPDRWGGLVDLPSTLDPHVRTQLCAALGGAHTGEDQLALRPVGILGRRLVP
DGGDRPAARTAPWRPAGTVLITGGTGALGAHVARWAAARGVGHLLLASRRGADAPGVAEL
RADLEASGASVTVAACDVADRQAVQQLLDGIPDRHPLEAVFHAAGVLDDGMTADLDTDRL
ETVLAPKTEGARLLDELTRDTELSAFVLFSGFAATVGSSGQAGYAAANAHLDALAQRRRA
DGLPATSIAWGPWTGGGLVDEGIEERLLRRGLTAMEPRLAVLGLSALLSDGPEGPANVAL
VDVDWRRFLSSFTASRPSPLLRELHQPSHADPGATDGRDATPGGGAGPTLAERLPGLTGA
ERDRLLLTTVRAQVAGTLGYPGPDAVSPRQQFKELGIDSLTALELRNGLQAVAGVALPAT
LVFDHPTPEAVAGHLSRTILGDGAHTSSSLFTEIERLGSALGSADLDEAERNLVATRLRS
LASRWDVGPSSGAGDVAQTLADADRAEVLAFIDRELGLS
selected fasta
>polyketide synthase [Plm4]
GTGTCGGCAGCAAGGGTAGCGAGCGGCTCGTTTCCTGTGGACCGGGGCTGGGATCTGGAC
GCGCTCTTCAGCGAGGCGGCGGACGGAAAAGCAGCGTCGTATGTTCGCGAGGGCGGATTT
CTCTACGACGCCGGATGGTTCGATGCCTCGTTCTTCGGGATATCTCCGCGCGAAGCCGTG
ACAATCGATCCCCAGCAGCGACAGCTGCTGGAAGTCGCCTGGGAAGCCTTGGAAAGGGCC
CGGATTTCTCCACAGGCCTTGCGCGGCAGCGACGTCGGCGTCTTTGCCGGGGCCATGAAC
CAGGACTACGCCGTCCGACTGCACGAGTCCATCGAGGATTTCGAGGGTTTCCTGACAACT
GGCAACACCGGCAGTGTGGTCTCCGGTCGGCTGTCCTACACCCTCGGTCTGGTCGGCCCG
GCCGTAACCGTCGACACCGCCTGTTCCTCGTCGCTCGTGACCATGCACCTGGCGGCCCGG
TCGCTGCGCAGTCGGGAATGCTCGCTGGCCCTTGCCGGCGGGGTGACGGTGATGTCGATG
CCCACGACATTCACGGAGTTCTGCAGGCAACGCAATCTTGCCCCCGACGGCCGGGCCAAG
TCCTTCGCCGCCGCTGCGGACGGCACCGCGTGGGCCGAGGGCGTCGGCGTACTCGTGCTG
GAGCGGTTGTCCGACGCCGAACGCAACGGCCATCCCGTCCTCGCGTTGATACGTGGATCG
GCGGTGAACCAGGACGGGGCCTCCAACGGCCTGTCCGCCCCGAACGGGCCCTCCCAGGAG
CGAGTCATCTGGCAGGCCTTGCGCGACGCCCGGCTGACGGCCGATGAGGTCGACGCGGTG
GAGGGCCATGCGACGGGGACGCGGCTGGGTGATCCGATCGAGGCGCAGGCGTTGTTGGCG
ACGTACGGCCAGGGCCGGTCCGCCGATCGGCCTTTGTGGCTGGGGTCGTTGAAGTCGAAC
ATCGGACATGCACAGGCTGCCGCGGGTGTCGGTGGCGTGATCAAGACGGTGATGGCGTTG
CGGCACGGGGTGCTGCCGCAGACGCTGCATGTGGATGCGCCGACGCCGAAGGTGGACTGG
TCGGCGGGCGAGGTGCGGTTGCTGACACAGCGGCGGGAGTGGCCGCGTGCGGGCCGGCCG
CGCCGGGCCGGTGTGTCCTCCTTCGGGGTGAGCGGCACCAATGCCCACCTCATCCTCGAA
GAAGCCCCACAAGCTCCTCCACACGACGCTGCCAGGGACCGCACGGACACCGGCGGCGGC
GCAACGCTGCCGTTGGTCCTCTCGGCCCGTACCGCCGCCGCCGTCCGGGAGCAGGCCCGC
AGGCTGCACAGCCACCTGACCGACCACCCCGAGCTCGTACCCGCTCAGGTCGCCGGCTCG
CTGGTGACGACCCGGAGCACGTTCGACCAACGGGCCGTCGTCCTCGGCACGGACCGGGCC
GAACTGCTGGACGGACTGGACAGTGTCGCCCAAGGCGTTCCGGACGCACGGACGGTCACC
GGTTCCGCCGTCGGCGGCCGCGAAGTGGTCTTCGTCTTCCCCGGTCAAGGAGGCCAGTGG
GCAGGCATGGCCCTGGAGCTGATGGAGGAGTTCCCGGTCTTCGCCGAGACCCTGCACTCC
TGCGCCGACGCCCTCGCCGACCACGTCGACTGGTCCCTGCTGGACGTCCTGCGCCAGGCT
GAGGGGGCGCCGGGCCTGGACCGGGTCGATGTGATCCAGCCAGTGCTGTTCTCGGTCACG
GTCGCCCTCGCCGATCTGTGGCGTTCCCTGGGCATCGAGCCCTCGGCCGTGGTGGGCAGT
TCCCTGGGCGAGATCGCGGCCGCGTACACGGCCGGCACGCTGTCGCTCGCCGACGCGACC
AAGGTGGCGGTGCTGCGCAGCCGGGCCCTGCTGGAACTGTCCGGACGCAGCGGCATGGTG
TCCGTTCCTCTCGGGCGAGTGCAGGTCGAGGAACTGATCGCCGCCTGGCCGGACCGGCTG
TTCGTCGCCGGGGTCAACAGCCCGTCCATCAGCGTGGTCTCCGGCGACAACGAGGCGGTG
GACGAGCTGCTGGCGGTCTGTGCCGAGCGGGGCGTACGTGCCCGGCGCGTCGCCACCGAC
TGCGCCTCCCACTACCCCGCCGTCGAAGCCCTCGAAGAGCGGCTGCTGACGGACCTGGCG
GACGTGGCACCGACGCCGGCGCGCATCGCCTTCCTGTCGACCGTCTCCGGGGAATCAGCG
GAGACCGAAACGGTCCCGGACGCCGCGTACTGGTACCGCAACACCCGCCGGACCGTCGAG
TTCGGACCGGCCGTCCAGCGGCTCGCCGCCACCGGGAGCAAGGTCTACATCGAGATCAGC
CCCCACCCGGTGCTCCAGGTGGCGCTGTCCGAGATCGTGGAGGGAGAGAGCCGGGAGGCA
GCCGTTCTCAGCACCTTGCGACGCAATACGAGCGACCGGCGGGCCTTCCTCACCTCCCTG
GCCAAGGCATACGTCAGCGGCGTCACCGTCGACTGGGCGGCGCTGCCGGACCTGGCCGGC
GCTCCGCCCGTCGACCTGCCCACGTACGCCTTTCAGCGCGAGCGCTACTGGCCCCGTCCG
GCGGCCGCGTCGAACGGCAGCCGTGGACCGGGGGCGGGCGCCCCCGCGACCGTCGGCCAA
GGGACGGTGGACGCCCACTTCTGGGAGGCCGTGGAGAACGGGGACCTGGGCGGCCTCGGT
CCGGACGTACGGTTCGACGACGAGACCCCGCTGAAGGACGTCCTCCCGGAGCTGGCCTCC
TGGCACCGCCAGGGGCTCGAACAGGCCAGAGTGGACGGCTGGCGGTACGTCGAGAAGTGG
CGGCCGCTCGACGTACCCGCCACCGGGCCGCACGGCAGGTGGCTGCTGGTCCACCCGGGC
GGCACGGAGTCCGACGCCACCACGGAATGGGTGCGGGACGGCCTGCTGGAGGCGGGTTGC
TCGGTCGACGACCTCCTGGTCGACACGACGGACACCGACACCGCGGCGGTCACCGAGCGC
CTGAAGCAGGCCTGCGCGGGCGGCGGGCCGGAGCCGGTCGGAGTGGTGTCCCTGCTGGCC
TTCGACGGGCGGCACGACACCGCGTGTCCCTCGGTGCCCCGGGGCACCGCGGCGACCCTC
GCCCTCGTCCGGGCGCTCGGCGCCGCCGGGATCACGGCACCCCTGTGGTGCCTGACGCAG
GGCGCGGTCACCACCGGAACCGGCGACAGGCTCGGTGCTGTCGAACAGGCCCAGATCTGG
GGCCTGGGCAGAGTGGTCGCGCTGGAACACCCCGACCGCTGGGGCGGACTCGTCGACCTG
CCGAGCACCCTCGACCCGCACGTACGAACCCAGCTGTGCGCGGCCTTGGGCGGCGCGCAC
ACGGGCGAAGACCAACTGGCGCTGCGCCCGGTCGGCATCCTCGGACGCCGACTCGTCCCG
GACGGCGGCGATCGCCCGGCCGCTCGGACCGCCCCCTGGCGGCCGGCCGGAACCGTCCTC
ATCACCGGCGGTACCGGCGCTCTGGGCGCCCACGTCGCCCGCTGGGCCGCCGCCCGGGGA
GTCGGCCATCTGCTTCTGGCCAGCCGACGCGGGGCCGACGCGCCCGGCGTGGCCGAGCTC
AGGGCCGATCTGGAGGCATCGGGCGCCAGCGTGACCGTCGCCGCCTGCGATGTCGCCGAC
CGCCAGGCCGTACAACAACTACTCGACGGCATTCCGGACCGGCATCCGCTGGAGGCGGTT
TTCCACGCTGCCGGAGTACTCGACGACGGCATGACCGCCGACCTGGACACCGACCGCCTG
GAAACCGTCCTGGCGCCGAAGACCGAGGGCGCCCGGCTGCTGGACGAACTCACCCGCGAC
ACCGAACTGTCGGCGTTCGTGCTGTTCTCCGGCTTCGCCGCCACCGTGGGCAGCAGCGGC
CAGGCCGGTTACGCCGCCGCCAACGCTCACCTCGACGCCCTGGCCCAGCGGCGTCGGGCC
GACGGACTGCCCGCCACCTCGATCGCGTGGGGGCCATGGACCGGAGGCGGTCTCGTGGAC
GAGGGCATCGAGGAGCGCCTGCTGCGGCGCGGCCTCACCGCCATGGAACCCCGTCTGGCC
GTCCTGGGGCTGTCCGCGCTGCTTTCCGACGGCCCCGAAGGCCCCGCGAACGTGGCGCTC
GTCGATGTCGACTGGCGGCGGTTCCTGTCCTCGTTCACCGCATCCCGGCCGAGCCCCCTG
CTGAGGGAACTGCACCAGCCCTCGCATGCGGACCCCGGCGCGACCGACGGGCGCGACGCC
ACACCCGGCGGCGGGGCCGGCCCCACACTGGCCGAACGCCTGCCGGGCCTCACCGGCGCG
GAGCGGGACCGGCTCCTGCTCACGACGGTCCGTGCCCAGGTGGCCGGCACGCTCGGGTAT
CCCGGGCCCGACGCCGTGTCCCCGCGGCAGCAGTTCAAGGAACTCGGTATCGATTCCCTC
ACCGCCCTCGAACTGCGCAACGGGCTACAGGCCGTGGCCGGAGTCGCTCTCCCGGCCACG
CTGGTCTTCGACCATCCGACACCGGAGGCGGTCGCCGGCCATCTGAGCCGGACCATCCTC
GGTGACGGGGCACACACGTCGTCCTCCCTCTTCACCGAGATCGAACGCCTCGGCTCCGCG
CTCGGCTCCGCCGACCTGGACGAAGCGGAACGGAACCTCGTCGCGACCCGACTGCGCTCG
CTGGCCTCCCGATGGGACGTCGGCCCGTCGAGCGGCGCCGGAGACGTCGCACAGACGCTG
GCGGACGCCGACCGGGCCGAGGTCCTCGCCTTCATCGACCGAGAGCTCGGCCTCTCGTAG
[4] KS4..352
[4] AT510..829
[4] ethylmalonyl-CoA not conserved XAGHS701..705
[4] KR1157..1336
[4] ACP1446..1516
[4] KS10..1056
[4] AT1528..2487
[4] ethylmalonyl-CoA not conserved XAGHS2101..2115
[4] KR3469..4008
[4] ACP4336..4548

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[503-633] 7.99999999999993e-68 G3DSA:3.40.366.10 [701-819] 7.99999999999993e-68 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1474-1489] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1446-1519] 4.80000000000001e-20 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1446-1516] PS50075
PS50075   ACP_DOMAIN
[1449-1515] 2.7e-11 PF00550
PF00550   PP-binding
[1437-1523] 7.60000011115812e-20 SSF47336
SSF47336   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[1157-1335] 1.2e-62 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[4-226] 2.39999999999997e-72 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[234-352] 7.89999999999997e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[510-829] 1.20000000000001e-98 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[508-813] 8.00002659346736e-58 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[635-700] 3.00000067992871e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[12-238] 4.69999999999992e-74 G3DSA:3.40.47.10 [239-404] 3.5e-62 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[11-350] 1.3000049540733e-80 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1157-1337] 1.40000000000001e-35 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[139-155] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[512-810] 2.1000026783403e-103 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1447-1519] 3.49999466863949e-31 SM00823
SM00823   PKS_PP
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1157-1336] 3.59998852907783e-57 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Phosl_00050 Phosl_00050   Phosl_00070 Phosl_00070
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