Phosl_00090 Phosl_00090   Phosl_00110 Phosl_00110

Phosl_00100 : CDS information

close this sectionLocation

Organism
StrainHK803
Entry namePhoslactomycin
Contig
Start / Stop / Direction31,679 / 33,394 / + [in whole cluster]
31,679 / 33,394 / + [in contig]
Location31679..33394 [in whole cluster]
31679..33394 [in contig]
TypeCDS
Length1,716 bp (571 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative 3-hydroxyacyl-CoA dehydrogenase
putative beta-keto-reductase
Product (GenBank)PlmT8
Gene
Gene (GenBank)plmT8
EC number1.1.1.35
Keyword
  • ethylmalonyl-CoA
Note
Note (GenBank)
  • oxidoreductase
Reference
ACC
PmId
[12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003)
comment
Phoslactomycins生合成gene clusterのクローニング、配列解析報告。

oxidoreductase (plmT8) と aminotransferase (plmT1)は、C-8 hydroxyethyl側鎖のC-8 hydroxyl基をamineで置換するのに関係しそうだと述べている。
Related Reference
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
comment
45th, 2hsp: N末側33%, C末側43%
Clostridium acetobutylicum_hbd
3-hydroxybutyryl-CoA dehydrogenase(EC 1.1.1.157)

BCS (butyryl-CoA synthesis) operon (crt-bcd-etfB-etfA-hbd)のクローニングとシークエンス。
crt geneの上流にあるプロモーターによって制御される。transcriptional unitであることを確認。

BCS operonをE. coliで大量発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase activity確認。

BCS operonをC. acetobutylicum で発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase activity上昇。

close this sectionSequence

selected fasta
>putative 3-hydroxyacyl-CoA dehydrogenase [PlmT8]
MAKRFSTIAVIGLGTTGSVLASMAARSGRRVIAVDTDASALDLAGARLTDTGPGTIDLTT
RSADIVSADLVIEAVPERMKTKCELLSHAHNACAPGAVFATTTSGLAVTDIAFGSGRPCR
TVGLHLFPQGPMDPATAVEVVGTPLTDGSVLADVQALIRDLGQVPVSVPDRAGFVGGALT
MAYLNDAATMYEQRYASRDGIDTAMRLGCGLPRGPLAHLDAIGLDVARDTLDALHERTGD
RRFLPAPVLTHMVAAGLLGLKAGRGFYDYGAPDADGAADTGDELPTARPARRIGVVGSGT
MATGIAQACARAGYPTTLVARSEVRAKEALATVENSLNRAVQRGRLTPEQLTSSMESLTG
VSRLEAVAACDLVVEAVVEDIDVKRTVFRELDAVCGAQTVLATSTSSLPVIECAMATGRP
EAVVGMHFFNPAPVMKLVEVVRTALTSRETLGVAHATATALGKRPVGCLDRSGFIVNALL
FPYLNRAVALLDEGILTAEGIDTVMALGQGHPMGPLQLLDVIGLDVSVAILRTLHTTFRE
QRLLPSRPLEQLVAAGHLGRKRGKGLRNHEL
selected fasta
>putative 3-hydroxyacyl-CoA dehydrogenase [PlmT8]
ATGGCTAAGCGCTTCTCCACCATCGCGGTGATAGGTCTCGGGACCACCGGGTCGGTGCTC
GCGAGCATGGCCGCCCGCAGCGGCCGCCGGGTGATCGCCGTCGACACCGACGCCTCCGCT
CTGGACCTGGCCGGTGCCCGGCTGACCGACACCGGACCGGGCACGATCGACCTCACCACG
CGCTCGGCCGACATCGTGTCGGCGGACCTGGTGATCGAAGCGGTACCCGAGCGGATGAAG
ACGAAGTGCGAGCTGCTCAGTCACGCGCACAACGCGTGCGCCCCGGGCGCGGTGTTCGCG
ACGACGACCAGCGGACTGGCGGTGACCGACATCGCCTTCGGCTCGGGGCGGCCATGTCGC
ACGGTGGGGCTGCACCTGTTCCCACAGGGGCCGATGGACCCGGCGACCGCCGTGGAGGTC
GTGGGTACGCCCCTGACGGACGGGTCCGTACTCGCGGACGTCCAAGCACTGATTCGAGAC
CTCGGGCAGGTCCCGGTGTCGGTGCCCGATCGCGCGGGCTTCGTCGGCGGGGCCCTGACT
ATGGCCTATCTCAACGACGCCGCGACGATGTACGAACAGCGCTACGCCTCGCGCGACGGC
ATCGACACCGCCATGAGGCTGGGCTGCGGGCTTCCGCGAGGGCCGCTGGCTCACCTGGAC
GCGATCGGGCTGGATGTCGCGCGTGACACGTTGGACGCCCTGCACGAACGCACCGGTGAC
CGCCGGTTCCTGCCCGCCCCCGTACTGACGCACATGGTCGCCGCCGGCCTGCTCGGGCTG
AAGGCCGGGCGTGGCTTCTACGACTACGGCGCCCCGGACGCCGACGGAGCCGCGGACACC
GGCGACGAGCTGCCGACGGCCCGCCCCGCACGGCGTATCGGCGTAGTGGGTTCGGGGACG
ATGGCCACCGGCATCGCGCAGGCCTGCGCACGGGCGGGGTACCCGACGACTCTCGTGGCG
CGCAGCGAGGTCCGCGCCAAAGAGGCGCTCGCCACCGTGGAGAACTCGCTGAACCGGGCG
GTGCAGCGGGGCAGACTGACACCGGAGCAGCTCACCTCGTCGATGGAGTCGCTCACCGGT
GTGTCCCGCCTGGAGGCGGTCGCCGCATGCGATCTCGTGGTCGAGGCGGTCGTGGAGGAC
ATCGACGTCAAGCGGACGGTGTTCCGGGAACTCGACGCCGTATGCGGGGCGCAGACCGTG
CTGGCGACATCCACGTCGAGTCTGCCGGTCATCGAATGTGCCATGGCGACCGGTCGGCCC
GAGGCCGTGGTCGGGATGCACTTCTTCAATCCGGCACCGGTGATGAAACTCGTCGAAGTG
GTGCGGACCGCGCTGACCTCGCGCGAGACACTCGGCGTGGCGCACGCGACCGCCACCGCG
CTCGGCAAGCGTCCTGTGGGCTGTTTGGACCGGAGCGGGTTCATCGTGAACGCGTTGCTG
TTCCCCTATCTGAACAGAGCGGTGGCCCTGCTCGACGAGGGGATCCTCACCGCCGAGGGC
ATCGACACGGTGATGGCCCTGGGACAGGGCCATCCCATGGGCCCGCTCCAGCTGCTGGAC
GTGATCGGTCTGGATGTGTCCGTGGCCATCCTGCGCACGCTGCACACCACGTTCCGGGAG
CAACGCCTCCTGCCCTCGCGCCCTCTTGAGCAGCTCGTGGCGGCCGGCCACCTGGGCCGC
AAGCGGGGCAAGGGACTGCGGAACCACGAGCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
[173-269] 4.9e-24 PF00725 [473-566] 7.20000000000003e-27 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
[7-170] 6.19999999999994e-38 PF02737 [293-470] 1e-57 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[173-270] 4.00000300869883e-27 SSF48179 [473-570] 5.10001002358244e-27 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
[175-269] 1.80000000000002e-56 G3DSA:1.10.1040.10 [475-565] 1.80000000000002e-56 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
[3-174] 1.29999999999998e-83 G3DSA:3.40.50.720 [285-474] 1.29999999999998e-83 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-21] 0.999 Signal
Bacteria, Gram-negative   
[1-33] 0.998 Signal
Bacteria, Gram-positive   
[1-21] 0.95 Signal
Eukaryota   
TMHMM No significant hit
Phosl_00090 Phosl_00090   Phosl_00110 Phosl_00110
Page top