Phosl_00110 : CDS information

Location

Organism	Streptomyces sp.
Strain	HK803
Entry name	Phoslactomycin
Contig	AY354515
Start / Stop / Direction	33,459 / 34,805 / + [in whole cluster] 33,459 / 34,805 / + [in contig]
Location	33459..34805 [in whole cluster] 33459..34805 [in contig]
Type	CDS
Length	1,347 bp (448 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative crotonyl-CoA reductase
Product (GenBank)	PlmT7
Gene
Gene (GenBank)	plmT7
EC number	1.3.1.-
Keyword	ethylmalonyl-CoA
Note
Note (GenBank)	crotonyl CoA reductase
Reference	ACC Q6V1N5 PmId [12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003) comment Phoslactomycins生合成gene clusterのクローニング、配列解析報告。 crotonyl-CoA reductase (plmT7)は4番目と6番目の伸長ステップで使用するethylmalonyl-CoA extender unitを供給するためにおそらく必要である。
Related Reference	ACC Q53865 PmId [8521864] Purification of crotonyl-CoA reductase from Streptomyces collinus and cloning, sequencing and expression of the corresponding gene in Escherichia coli. (Eur J Biochem. , 1995) comment 20th, 71%, 0.0 Streptomyces collinus_ccr Crotonyl CoA reductase 分子量からおそらくhomodimerで機能していると考えられる。 E.coliでcloning､発現､精製し、活性をみている。crotonyl-CoAとenoyl-CoA（C4）には高い基質特異性を見せるが、enoyl-CoA thioester, acryloyl-CoAのような短鎖(C3)には測定可能な活性はみられなかった。
	ACC Q9RNU6 PmId [10542184] Role of crotonyl coenzyme A reductase in determining the ratio of polyketides monensin A and monensin B produced by Streptomyces cinnamonensis. (J Bacteriol. , 1999) comment 36th, 69%, 0.0 Streptomyces cinnamonensis_ccr Crotonyl-CoA reductase S. cinnamonensis_ccr、そのS. cinnamonensisで異種性発現させたS. collinus_ccr、ccr-disrupted mutantなどでCCR activity測定あり。 monensin A/monensin Bの比率は、CCR活性のレベルと、CCRの基質であるcrotonyl-CoAの両方に依存する。
	ACC B8XVS5 PmId [17548827] Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. (Proc Natl Acad Sci U S A. , 2007) [19458256] Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase. (Proc Natl Acad Sci U S A. , 2009) comment 78th, 42%, 5e-77 Rhodobacter sphaeroides_ccr Crotonyl-CoA carboxylase/reductase この酵素だけでcrotonyl-CoAのreductionとreductive carboxylationが触媒されることを確かめている。 crotonyl-CoA＋NADPH＋CO2 → ethylmalonyl-CoA-＋NADP+

Sequence

>putative crotonyl-CoA reductase [PlmT7]
MREIVSAALSDNAVGADFAALPVPQSYRGAVVLRDESEMFQGMATADKEPTKSLSVREVP
TPEPAPGEALIAVMASSINYNTVWTSLFEPMPTFGFLQRYARSNEPGAERHDQPYHVVGS
DLAGIVLRTGPGVHRWKPGDRVVAHCLSVDLQSPDGHDDTMLDPEQRSWGFETNFGGLAE
LSLVKSNQLMPMPKHLTWEEAAASGLVNSTAYRQLVSRNGGHMKQGDVVLVWGAAGGLGS
YATQLALNGGATPVCVVSGERKAQLVRSLGAELVIDRAKEGYRFWKDESTPDPKEWKRLG
SHIRELTGGEDSDIVFEHPGRETFAASVYVARRGGTVVTCASTSGFRHEYDNRYLWMSLK
RIVGSHFANYREAWAANRLIGRGMIHPTLSTVYPLSDVGIATQAVHRNQHAGKVGVLGLA
PAEGLGVQDEALRERHLAAINRFRSRGS

>putative crotonyl-CoA reductase [PlmT7]
ATGCGGGAGATCGTGAGCGCTGCCCTGTCGGACAATGCGGTCGGTGCGGATTTCGCCGCG
TTGCCGGTTCCGCAGTCCTACCGCGGTGCCGTAGTCCTGCGGGACGAGTCGGAGATGTTC
CAGGGTATGGCGACCGCCGACAAGGAACCCACGAAGTCCCTCAGCGTGCGCGAGGTGCCG
ACTCCCGAGCCCGCTCCGGGCGAGGCCCTCATCGCGGTGATGGCCAGCTCCATCAACTAC
AACACCGTGTGGACCTCCCTCTTCGAGCCGATGCCGACCTTCGGTTTCCTGCAGCGGTAC
GCGCGGTCCAACGAACCTGGCGCGGAGCGACACGATCAGCCCTACCACGTGGTGGGTTCC
GACCTGGCGGGCATCGTGCTGCGTACCGGGCCCGGCGTGCACCGCTGGAAGCCGGGCGAC
CGGGTCGTGGCGCACTGCCTGTCCGTCGACCTGCAGTCCCCGGACGGGCACGACGACACC
ATGCTCGACCCGGAGCAGCGCAGCTGGGGGTTCGAGACCAACTTCGGGGGGCTCGCCGAG
CTGTCCCTCGTCAAGTCCAACCAGCTCATGCCGATGCCCAAGCACCTCACGTGGGAGGAG
GCCGCTGCCTCCGGGCTGGTCAACTCCACCGCCTACCGGCAACTCGTCTCGCGCAACGGT
GGGCACATGAAGCAGGGCGACGTGGTTCTCGTCTGGGGCGCGGCGGGCGGCCTCGGCTCG
TACGCCACGCAGCTCGCCCTCAATGGCGGCGCGACTCCGGTGTGCGTCGTGTCCGGAGAG
CGCAAGGCCCAACTCGTCCGGTCCCTGGGCGCCGAGCTCGTGATCGACCGGGCGAAGGAG
GGGTACCGGTTCTGGAAGGACGAATCCACGCCCGACCCCAAGGAGTGGAAGCGCCTGGGC
TCCCACATCCGGGAGCTGACAGGGGGTGAGGACTCGGACATCGTCTTCGAGCACCCCGGC
CGGGAGACCTTCGCCGCCAGCGTGTACGTGGCGCGGCGCGGCGGCACCGTGGTCACCTGC
GCCTCGACCTCCGGATTCCGGCACGAGTACGACAACCGCTATCTCTGGATGTCGCTCAAG
CGCATCGTCGGCAGCCACTTCGCCAACTACCGGGAGGCCTGGGCGGCCAACCGTCTCATC
GGCAGGGGGATGATCCATCCGACCCTGTCCACGGTGTACCCGCTGTCCGACGTCGGCATC
GCCACGCAGGCCGTGCACCGCAATCAGCACGCCGGCAAGGTCGGAGTGCTGGGCCTGGCG
CCCGCGGAGGGGCTCGGCGTCCAGGACGAGGCGCTGCGGGAGCGCCACCTGGCAGCGATC
AACCGCTTCCGCAGCCGCGGCAGTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010085 Crotonyl-CoA reductase (Family) TIGR01751 Crot-CoA-red IPR011032 GroES-like (Domain) SSF50129 GroES_like IPR013149 Alcohol dehydrogenase, C-terminal (Domain) PF00107 ADH_zinc_N IPR013154 Alcohol dehydrogenase GroES-like (Domain) PF08240 ADH_N IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Phosl_00100 Phosl_00120