Phosl_00110 Phosl_00110   Phosl_00130 Phosl_00130

Phosl_00120 : CDS information

close this sectionLocation

Organism
StrainHK803
Entry namePhoslactomycin
Contig
Start / Stop / Direction34,851 / 35,081 / + [in whole cluster]
34,851 / 35,081 / + [in contig]
Location34851..35081 [in whole cluster]
34851..35081 [in contig]
TypeCDS
Length231 bp (76 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)PlmT6
Gene
Gene (GenBank)plmT6
EC number
Keyword
Note
Note (GenBank)
  • ferredoxin
Reference
ACC
PmId
[12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003)
comment
Phoslactomycins生合成gene clusterのクローニング、配列解析報告。

fig2:
plmT6はpostpolyketide modificationに関連する酵素として図示されている。
Related Reference
ACC
P18325
PmId
[1846297] Ferredoxins from two sulfonylurea herbicide monooxygenase systems in Streptomyces griseolus. (Biochemistry. , 1991)
comment
7th, 55%, 1e-10
Streptomyces griseolus_subB
Ferredoxin-2
metal-ion binding site保存あり

3Fe-4S ferredoxin.
sulfonylurea herbicideが誘発されたStreptomyces griseolusの可溶性タンパク質分画からFd-1 と Fd-2の二種類のフェレドキシンを精製し、どちらも、aerobic mixture of NADPH、spinach ferredoxin:NADP oxidoreductase、精製したcytochrome P-450SU1とherbicide基質にsulfonylurea monooxygenase活性を復元できることが示されている。また、cytochromes P-450, P-450SU1 (CYP105A1) と P-450SU2 (CYP105B1)は、それぞれFd-1 と Fd-2の近傍に位置していることが示されている。

close this sectionSequence

selected fasta
>putative ferredoxin [PlmT6]
MAFAHVHLDESKCVTSGQCVLTAPEVFDQRETDGVGVVLTDSPAPELRDKVQEAAAGCPA
AAIRLLHRPPGRVAGG
selected fasta
>putative ferredoxin [PlmT6]
ATGGCGTTTGCGCATGTGCACCTCGACGAATCGAAGTGCGTCACCTCGGGGCAGTGCGTC
CTGACCGCCCCCGAGGTGTTCGATCAGCGGGAGACGGACGGTGTCGGCGTCGTGCTGACC
GACTCCCCCGCCCCGGAACTGCGCGACAAGGTGCAGGAGGCCGCGGCCGGCTGCCCCGCG
GCGGCGATCCGACTGCTGCACCGGCCCCCCGGACGGGTGGCCGGCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001080 3Fe-4S ferredoxin (Family)
[8-19] 2.00000075217457e-06 PR00352 [19-29] 2.00000075217457e-06 PR00352 [51-63] 2.00000075217457e-06 PR00352
PR00352   3FE4SFRDOXIN
SignalP No significant hit
TMHMM No significant hit
Phosl_00110 Phosl_00110   Phosl_00130 Phosl_00130
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